GEOMETRIC, VOLUMETRIC, COLOUR AND FRICTIONAL PROPERTIES OF SELECTED SALVIA SPECIES OF TURKEY.

BACKGROUND: Salvia seeds are promite to dietary and healthy oils because they contain essential fatty acids. Salvia seeds frequently produce mucilage on soaking, and this mucilage is used for the treatment of eye diseases in eastern countries. Salvia species studied for medicine, food and cosmetics, have the potential to be used in the various fields. In the present study, selected engineering (geometrical, volumetrical, colour and frictional) properties of 6 Salvia species seeds were determined and compared. MATERIALS AND METHODS: This study was performed on selected engineering properties of seeds of 6 Salvia species (Salvia viridis L., Salvia aethiopis L., Salvia cryptantha Montbert & Aucher ex Benthan., Salvia tomentosa Mill., Salvia sclarea L., Salvia virgata Jacq.,) cultivated in Turkey. Plants were collected during the vegetation in 2012-2013 (May-Agust). The seeds were cleaned from foreign matter, dirt and broken seeds manually. RESULTS: The average length, width and thickness were found in the range of 2.61 to 3.53 mm, 1.59 to 2.92 mm and 1.14 to 2.52 mm, respectively. Salvia viridis L. specie had the lowest geometric mean diameter and surface area, whereas Salvia cryptantha L. had the least values among these 6 Salvia species for these properties. The bulk density, true density and the porosity were between 296.83 and 702.80 kg m-3, 285.69 and 718.08 kg m-3, 10.27 and 44.05%, respectively. The volume of unit seed and sphericity ranged between 2.56 and 13.64 mm3, 62.90 and 90.40%, respectively. The coefficient of friction of salvia species were largely influenced by the friction surfaces studied, and highest values were found for polywood in the Salvia crytantha L.. In the study, the static friction coefficient and the angle of repose of salvia species changed from 0.477 to 0.955, and from 14.09 to 23.57°, respectively. CONCLUSION: Determination of geometric, volumetric, colour and frictional properties of Salvia spp. seeds may increase their economic value.

[Herbal textural and original plants research on medicines from Salvia in China].

On the basis of review and study on literatures of Chinese medicine, combining the history of medicinal plants of Salvia in China, and investigating the morphological description, geography distribution and therapeutic effects, herbal textural research were carried on the medicinal plants from Salvia. The results showed that the original plant of Danshen is S. miltiorrhiza and related species, the original plant of Dian Danshen is S. yunnanensis, the original plant of Li Zhi Cao is S. plebeia, the original plant of Shi Jian Chuan is Bidens pilosa. or S.chinensis, the original plant of Shu Wei Cao is S. japanica, the original plant of Ye Xia Hong is S. kiangsiensis. And the article aimed to point out the relationship of medicinal plants from Salvia and provided new insight and proof to explore the new natural medicine from medicinal plants of Salvia.

[Anatomical characteristics of laminae and petioles of 11 species of Salvia and their taxonomic significance].

Anatomical features of the lamina and petiole of 11 species of Salvia had been compared. The microstructures of 11 species of Salvia were observed by paraffin method, and indexes such as the epidermis, xylems and phloems were measured. The data set was analyzed by principal components analysis method and cluster analysis method. The results indicated that the laminae of 11 species of Salvia were bifacial leaf, and the surface of laminae and petioles were covered with villous. The upper epidermis and lower epidermis of the laminae of 11 species of Salvia were constituted by a dense cuticular layer without stratum corneum. Differences existed in the structure of cross sectio mn of laminae and petioles of 11 species of Salvia. Among them, upper epidermal thickness of nine different micromorphological characters could serve as one of the criteria to identify species of taxonomic Salvia. The identification of genetic relationship of these characters in Salvia were discussed in the paper to provide an evidence of anatomy in Salvia.

Vegetative anatomy and micromorphology of Salvia divinorum (Lamiaceae) from Mexico, combined with chromatographic analysis of salvinorin A.

Salvia divinorum--a species traditionally cultivated in Oaxaca, Mexico--possesses hallucinogenic properties. It is legally recognized as a controlled substance and prohibited in many countries. The proper identification of the plant, both in fresh and dried forms, is an important issue in crime-prevention campaigns. This paper provides a thorough anatomical description of leaves, petioles, and stems of S. divinorum. Detailed investigation of foliar trichomes was performed and illustrated. In addition, chromatographic analyses, including TLC and HPLC, were applied to fresh and dried plant material, together with the standard reference salvinorin A. A comprehensive identification method for S. divinorum based on a thorough anatomical examination is proposed, combined with chemical analysis for proper plant recognition.

Morphoanatomical and phytochemical studies of Salvia lachnostachys (Lamiaceae).

Salvia lachnostachys Benth., Lamiaceae, is a endemic species from southern Brazil. The essential oil of its leaves and flowers is mainly constituted by aliphatic compounds, such as dodecanoic acid, with sesquiterpenes as minor constituents. This work evaluated the morphology, anatomy, microchemistry, and phytochemistry of S. lachnostachys to provide advanced knowledge of Brazilian plants with medicinal potential. Light and scanning electron microscopy techniques were used in the anatomical and microchemical studies. Compounds were isolated by chromatographic techniques, identified by analysis of their NMR spectra and compared with published data. S. lachnostachys can be distinguished from other related species mainly by its petiolate leaves, terminal inflorescence, persistent bracts, and villous-glandular corolla. The stem and leaves of S. lachnostachys display anatomical characteristics common to the family Lamiaceae. However, this species can be distinguished from other family members by the morphology and the presence of eglandular and glandular trichomes, as well as the organization of the vascular bundles of the petiole. The phytochemical results revealed that S. lacnostachys produces oleanolic and ursolic acids in addition to the diterpene fruticuline A, which is a rare compound, previously found only in Salvia fruticulosa Benth. and S. corrugata Vahl. Ursolic and oleanolic acids are bioactive triterpenes that exhibit antiatherosclerotic, anticancer, antihypertensive, antinflammatory, antileukemic, antimutagenic, antioxidant, antiproliferative, and antiviral activities, and fruticuline A has antibacterial activity.

Morphological characterisation and agronomical parameters of different species of Salvia sp. (Lamiaceae).

The aim of this work is to assess the morphological characteristics and parameters of biomass production, such as fresh and dry matter weight (FMW and DMW, g/plant), yield of dry matter (YDM) in terms of ton/ha, essential oil content (EOC, mL/100 g) and yield of essential oils (YEO) expressed as L/ha of the following plants Salvia verbenaca, Salvia argentea, Salvia lavandulifolia, Salvia pratensis, Salvia sclarea, Salvia triloba and Salvia officinalis. Except for Salvia argentea (S2) all other species have adapted to the south Brazilian climate conditions, with morphological differences among the species evaluated. In terms of DMW and YDM, S. officinalis was found to be the most productive species with 445.83 g/plant and 11.14 ton/ha. The higher essential oil content and yield was observed for S. officinalis, affording 1.99 mL/100 g and 221.74 L/ha, respectively. Chemical characterisation of the essential oils obtained from hydrodistillation was performed through GC and GC/MSD analyses, which revealed for most of the species studied, α e ß-thujone, camphor and 1,8-cineole as major compounds, apart from S. sclarea, for which linalool, linalyl acetate and α-terpineol were the major components.

Hairy root cultures for secondary metabolites production.

Hairy roots (HRs) are differentiated cultures of transformed roots generated by the infection of wounded higher plants with Agrobacterium rhizogenes. This pathogen causes the HR disease leading to the neoplastic growth of roots that are characterized by high growth rate in hormone free media and genetic stability. HRs produce the same phytochemicals pattern of the corresponding wild type organ. High stability and productivity features allow the exploitation of HRs as valuable biotechnological tool for the production of plant secondary metabolites. In addition, several elicitation methods can be used to further enhance their accumulation in both small and large scale production. However, in the latter case, cultivation in bioreactors should be still optimized. HRs can be also utilised as biological farm for the production of recombinant proteins, hence holding additional potential for industrial use. HR technology has been strongly improved by increased knowledge of molecular mechanisms underlying their development. The present review summarizes updated aspects of the hairy root induction, genetics and metabolite production.

Chemical composition and biological activities of essential oil from Salvia sclarea plants regenerated in vitro.

The essential oils obtained by hydrodistillation of dried aerial parts of Salvia sclarea L. plants, regenerated in vitro and reproduced from seeds, were analyzed by GC and GC-MS. The oils from in vitro and in vivo plants were compared in respect to their chemical composition as well as antimicrobial and cytotoxic activities. The chemical profiles of both oils were very similar, although the yield of essential oil from in vitro plants was lower (0.1%, v/w) than the oil yield isolated from in vivo S. sclarea plants (0.2%, v/w). Both oils showed antimicrobial and cytotoxic activity. The oil from in vitro regenerated plants of S. sclarea exhibited stronger cytotoxic action against NALM-6 cell lines in comparison with the essential oil from in vivo plants.

Chemical composition of essential oil and leaf anatomy of Salvia bertolonii Vis. and Salvia pratensis L. (Sect. Plethiosphace, Lamiaceae).

The taxonomical relationship between Salvia pratensis and S. bertolonii has been unclear for a long time. Salvia bertolonii has alternatively been considered a synonym, a subspecies, a problematic subspecies and a form of Salvia pratensis. However, both these two species are sometimes used in traditional medicine instead of sage (Salvia officinalis) or as an adulteration for the same drug. In order to confirm the status of S. bertolonii, together with the potential identification characteristics for differentiation from sage, both taxa were analyzed through the analysis of their essential oils, together with the micromorphological characteristics of the leaf surface and the anatomy and morphology of the leaves. The obtained results show that there are clear differences in the quantity of essential oil (0.073% for S. pratensis and 0.0016% for S. berolonii). The major compound in the essential oil of S. pratensis was E-caryophyllene (26.4%) while in S. berolonii essential oil caryophyllene oxide was the major component (35.1%). The micromorphological differences are also pronounced in the leaf indumentum (density and distribution of certain types of non-glandular and glandular trichomes). Clear distinction between the investigated Salvia species is also observed in the leaf anatomy (in S. pratensis leaves are thinner, palisade tissue is made of 1-2 layers of cells, and leaves of S. bertolonii are characterized by 2-3 layers of palisade tissue cells, and consequently thicker).

South African Salvia species: a review of biological activities and phytochemistry.

The genus Salvia (sage) belongs to the Lamiaceae and encompasses 900 species worldwide of which ca. 26 indigenous species are found in southern Africa. Salvia is the largest genus in this family and constitutes almost one quarter of the Lamiaceae. In South Africa, the majority of Salvia species are distributed predominantly in the Cape region. Salvia species are used in many parts of the world to treat various conditions. Many sages, if not all, form an integral part of traditional healing in South Africa particularly in regions where they occur in abundance. Several species are used to treat microbial infections, cancer, malaria, inflammation, loss of memory and to disinfect homes after sickness. Despite the extensive traditional use and the general interest in phytoconstituents of Salvia it remains ironic that research on the South African counterparts has until recently been neglected. The review aims to collate recent research results on the phytochemistry and pharmacological properties of indigenous species. Bio-active compounds with antiplasmodial and antibacterial activities have been isolated and structurally elucidated from Salvia chamelaegnea, Salvia radula and Salvia verbenaca. The essential oil composition of Salvia showed the dominance of monoterpene hydrocarbons, oxygen-containing monoterpenes and oxygen- containing sesquiterpenes. Salvia runcinata is identified as an alternative source of natural alpha-bisabolol. Many pharmacological activities are summarised (anti-oxidant, antimicrobial, antiplasmodial, analgesic, antipyretic, anticancer, anti-inflammatory and antinociceptive) as a first attempt to provide scientific support for past and present local traditional uses.

Chemical analysis of some Salvia species native to West Azarbaijan (Iran).

Present survey was performed on nine species of Salvia (Lamiaceae) growing wild in different regions of West Azarbaijan in Iran (S. limbata, S. vertisillata, S. macrochlamys, S. nemorosa, S. ceratophylla, S. candidissima, S. syriaca, S. multicaulis, S. sclarea). In this research, three of the most important biochemical characters such as total protein content, total oil content and fatty acids combination in seeds of these species were considered. Analysis of species average comparison variance conducted with ANOVA and Duncan's test (p < 0.05) revealed that there is a significant difference about protein content, oil content and fatty acid composition among species.

[Study on induction of polyploidy in Salvia bowleyana by colchicine treatment].

OBJECTIVE: To explore the technique of induction of polyploidy in Salvia bowleyana by colchicine treatment. METHOD: The three kinds of explant of bud, leaf and calli were induced by colchicine treatment. RESULT: The induction effects were better when the calli was treated by colchicines (15 mg x L(-1)) and the leaf was pre-cultured for one week. The doubling rate was 33.33%, while the majority were wholy doubled plants, and the leaves were thicker and broader, the color was darker, the root was thicker and the stoma size was obviously bigger than the diploid plants. The number of chromosome were 8 to 64. Isoenzyme analysis showed that the enzyme activities between the polyploid and the diploid plants were quite different. CONCLUSION: Induction of polyploidy by colchicine treatment is efficacious. The part of the doubled plants were identified as homologmous tetraploids.

Consequences of floral complexity for bumblebee-mediated geitonogamous self-pollination in Salvia nipponica Miq. (Labiatae).

I address how floral complexity influences geitonogamous self-pollination through manipulation of pollinator behavior in Salvia nipponica. The pivoting stamens of S. nipponica hinder nectar-collecting bumblebees from crawling into flowers, increasing the probing time per flower. I predicted that longer probing times would reduce the relative cost of moving between plants, causing bees to leave plants earlier. To test this prediction, I simplified S. nipponica flowers by removing the stamens from all open flowers within a 75-m2 quadrat. Bumblebees probed these flowers more quickly than intact flowers, but the stamen removal affected neither the frequency of flower revisitation nor the flight distance between plants. In response to the decrease in the probing time per flower, bees probed more flowers on these plants. Therefore, in S. nipponica, floral complexity reduces the opportunity for geitonogamous self-pollination. Stamen removal also increased bee visitation per flower, suggesting that this sort of complexity deters visitation. To keep complex flowers attractive, therefore, selection might increase floral rewards or longevity. Floral complexity might evolve in an integrative manner with the rest of the floral phenotype.

[Textural research on "Shidachuan" and "Shijianchuan" in "Ben Cao Gang Mu Shi Yi" (a supplement to the compendium of materia medica)].

OBJECTIVE: To investigate the historical origins of folk herbal medicines "Shidachuan" and "Shijianchuan" METHOD: The origins of "Shidachuan" and "Shijianchuan" were confirmed by textural research and investigation of therapeutic effects. RESULTS: In "Ben Cao Gang Mu" (the Compendium of Materia Medica) "Shidachuan" was not recorded, and "Shijianchuan" was listed only as a medicine of "nomination without use", with the habitat and morphology being blank. CONCLUSION: The "Shidachuan" which was originally recorded in "Ben Cao Gang Mu Shi Yi" (A Supplement to the Compendium of Materia Medica) should be "Longyacao" (Agrimonia pilosa) of Rosaceae family. "Shijianchuan" should be "Guizhencao" (Bidens bipinata) of Compositae family instead of "Huashuweicao" (Salvia chinensis) of Labiatae family. The herbal medicine recorded as "shijianchuan" in "Chinese Pharmacopoeia" is the very species of Salvia chinensis, which is considered as a new rising species of "Shijianchuan".