ABSTRACT
The disposal of spent nuclear fuel in deep subsurface repositories using multi-barrier systems is considered to be the most promising method for preventing radionuclide leakage. However, the stability of the barriers can be affected by the activities of diverse microbes in subsurface environments. Therefore, this study investigated groundwater geochemistry and microbial populations, activities, and community structures at three potential spent nuclear fuel repository construction sites. The microbial analysis involved a multi-approach including both culture-dependent, culture-independent, and sequence-based methods for a comprehensive understanding of groundwater biogeochemistry. The results from all three sites showed that geochemical properties were closely related to microbial population and activities. Total number of cells estimates were strongly correlated to high dissolved organic carbon; while the ratio of adenosine-triphosphate:total number of cells indicated substantial activities of sulfate reducing bacteria. The 16S rRNA gene sequencing revealed that the microbial communities differed across the three sites, with each featuring microbes performing distinctive functions. In addition, our multi-approach provided some intriguing findings: a site with a low relative abundance of sulfate reducing bacteria based on the 16S rRNA gene sequencing showed high populations during most probable number incubation, implying that despite their low abundance, sulfate reducing bacteria still played an important role in sulfate reduction within the groundwater. Moreover, a redundancy analysis indicated a significant correlation between uranium concentrations and microbial community compositions, which suggests a potential impact of uranium on microbial community. These findings together highlight the importance of multi-methodological assessments in better characterizing groundwater biogeochemical properties for the selection of potential spent nuclear fuel disposal sites.
Subject(s)
Desulfovibrio , Groundwater , Uranium , Bacteria , Uranium/analysis , RNA, Ribosomal, 16S/genetics , Prospective Studies , Groundwater/chemistry , Sulfates/analysisABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: Laetiporus sulphureus has long been used as an edible and medicinal mushroom in Asia, America, and Europe. Its fruiting bodies are widely used in folk medicine for treating cancer, gastric diseases, cough, and rheumatism. Polysaccharides are an important bioactive component of mushrooms. In nature, sulfated polysaccharides have never been reported in mushrooms. Furthermore, there is no information on differences in physicochemical properties and anti-breast cancer activities between polysaccharides (PS) and sulfated polysaccharides (SPS) of L. sulphureus. AIM OF THE STUDY: This study aimed to investigate the physicochemical properties of PS and SPS isolated from fruiting bodies of L. sulphureus and examine their anti-proliferative effects and mechanism(s) of action on MDA-MB-231 breast cancer cells. METHODS: Polysaccharides (PS) were isolated using hot water and ethanol precipitation methods. Sulfated polysaccharides (SPS) were isolated by the papain-assisted hydrolysis method. Physicochemical properties comprising sugar, protein, uronic acid, and sulfate contents, and molecular weight, monosaccharide composition, and structural conformation were analyzed on PS and SPS. In the anti-cancer study, a triple-negative breast cancer cell line (MDA-MB-231) and a normal human mammary epithelial cell line (H184B5F5/M10) were used to evaluate the anti-proliferative activity of PS and SPS, and their mechanism(s) of action. RESULTS: The results showed that SPS, which had higher sulfate and protein contents and diversified monosaccharide composition, exhibited more potent anti-proliferative activity against MDA-MB-231 cells than PS. Furthermore, it had a selective cytotoxic effect on breast cancer cells but not the normal cells. SPS induced cell cycle arrest at G0/G1 phase via down-regulating CDK4 and cyclin D1 and up-regulating p21 protein expression. Breast cancer cell apoptosis was not observed until 72 h after SPS treatment. In addition, SPS also markedly inhibited breast cancer cell migration. CONCLUSION: This study demonstrates that SPS exhibited selective cytotoxicity and was more potent than PS in inhibiting MDA-MB-231 cell proliferation. The contents of sulfate and protein, and monosaccharide composition could be the main factors affecting the anti-breast cancer activity of L. sulphureus SPS.
Subject(s)
Agaricales , Breast Neoplasms , Triple Negative Breast Neoplasms , Humans , Female , Sulfates/analysis , Cell Cycle Checkpoints , Breast Neoplasms/drug therapy , Breast Neoplasms/metabolism , Polysaccharides/pharmacology , Polysaccharides/therapeutic use , Polysaccharides/analysis , Apoptosis , Triple Negative Breast Neoplasms/drug therapy , Fruiting Bodies, Fungal/chemistry , Cell Movement , Monosaccharides/analysis , Cell Line, Tumor , Cell CycleABSTRACT
Bile acids (BAs) are a complex class of metabolites that have been described as specific biomarkers of gut microbiota activity. The development of analytical methods allowing the quantification of an ample spectrum of BAs in different biological matrices is needed to enable a wider implementation of BAs as complementary measures in studies investigating the functional role of the gut microbiota. This work presents results from the validation of a targeted ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the determination of 28 BAs and six sulfated BAs, covering primary, secondary, and conjugated BAs. The analysis of 73 urine and 20 feces samples was used to test the applicability of the method. Concentrations of BAs in human urine and murine feces were reported, ranging from 0.5 to 50 nmol/g creatinine and from 0.012 to 332 nmol/g, respectively. Seventy-nine percent of BAs present in human urine samples corresponded to secondary conjugated BAs, while 69% of BAs present in murine feces corresponded to primary conjugated BAs. Glycocholic acid sulfate (GCA-S) was the most abundant BA in human urine samples, while taurolithocholic acid was the lowest concentrated compound detected. In murine feces, the most abundant BAs were α-murocholic, deoxycholic, dehydrocholic, and ß-murocholic acids, while GCA-S was the lowest concentrated BA. The presented method is a non-invasive approach for the simultaneous assessment of BAs and sulfated BAs in urine and feces samples, and the results will serve as a knowledge base for future translational studies focusing on the role of the microbiota in health.
Subject(s)
Bile Acids and Salts , Tandem Mass Spectrometry , Humans , Mice , Animals , Bile Acids and Salts/analysis , Tandem Mass Spectrometry/methods , Sulfates/analysis , Chromatography, High Pressure Liquid/methods , Feces/chemistryABSTRACT
The purpose of this study is to explore the effects of pine pollen polysaccharides and sulfated polysaccharides on mice with ulcerative colitis and whether they could protect mice from inflammation by regulating the tight junctions of colonic epithelial cells and regulating the RIPK3-dependent necroptosis pathways. Pine pollen polysaccharides were prepared by water boiling and ethanol precipitation. After deproteinedization with trichloroacetic acid, the UV spectrum showed that there were no proteins. One polysaccharide component (PPM60-III) was made by gel filtration chromatography, and then sulfated polysaccharide (SPPM60-III) was derived using the chlorosulfonic acid-pyridine method. After treatment with PPM60-III and SPPM60-III, the body weight of mice with ulcerative colitis induced by dextran sodium sulfate increased, the DAI score decreased, the levels of pro-inflammatory factors and inflammation-related enzymes decreased, and the level of anti-inflammatory factors increased. In addition, after treatment, the expressions levels of tight junction proteins increased, the expressions levels of key proteins of programmed necroptosis decreased, while the level of Caspase-8 increased. The results indicated that pine pollen polysaccharides and sulfated polysaccharides have a certain therapeutic effect on UC mice, and the therapeutic effect may be achieved by regulating the tight junction of colonic epithelial cells and regulating the RIPK3-dependent necroptosis pathways.
Subject(s)
Colitis, Ulcerative , Necroptosis , Mice , Animals , Tight Junctions , Sulfates/analysis , Dextran Sulfate/adverse effects , Colitis, Ulcerative/chemically induced , Colitis, Ulcerative/drug therapy , Polysaccharides/chemistry , Sulfur Oxides , Inflammation , Pollen/chemistry , Receptor-Interacting Protein Serine-Threonine KinasesABSTRACT
There is a previously neglected influence of geochemical conditions on plant phytochemistry. In particular, high concentrations of dissolved salts can affect their biosynthesis of natural products. Detoxification is most likely an important aspect for the plant, but additional natural products can also give it an expanded range of bioactivities. During the phytochemical analysis a Palicourea luxurians plant collected in a sulfate-rich environment (near the Río Sucio, Costa Rica) showed an interesting natural product in this regard. The structure of this compound was determined using spectroscopic and computational methods (NMR, MS, UV, IR, CD, optical rotation, quantum chemical calculations) and resulted in a megastigmane sulfate ester possessing a ß-ionone core structure, namely blumenol C sulfate (1, C13H22O5S). The levels of sulfur and sulfate ions in the leaves of the plant were determined using elemental analysis and compared to the corresponding levels in comparable plant leaves from a less sulfate-rich environments. The analyses show the leaves from which we isolated blumenol C sulfate (1) to contain 35% more sulfur and 80% more sulfate than the other samples. Antimicrobial and antioxidant activities of compound 1 were tested against Escherichia coli, E. coli ampR and Bacillus subtilis as well as measured using complementary in vitro FRAP and ATBS assays, respectively. These bioactivities are comparable to those determined for structurally related megastigmanes. The sulfur and sulfate content of the plant leaves from the sulfate-rich environment was significantly higher than that of the other plants. Against this background of salt stress, we discuss a possible biosynthesis of blumenol C sulfate (1). Furthermore, there appears to be no benefit for the plant in terms of extended bioactivities. Hence, the formation of blumenol C sulfate (1) probably primarily serves the plant detoxification process.
Subject(s)
Biological Products , Rubiaceae , Rubiaceae/chemistry , Norisoprenoids/analysis , Sulfates/analysis , Escherichia coli , Plant Leaves/chemistry , Biological Products/analysis , Sulfur/analysisABSTRACT
Marine dissolved organic matter (DOM) is a major reservoir that links global carbon, nitrogen, and phosphorus. DOM is also important for marine sulfur biogeochemistry as the largest water column reservoir of organic sulfur. Dissolved organic sulfur (DOS) can originate from phytoplankton-derived biomolecules in the surface ocean or from abiotically "sulfurized" organic matter diffusing from sulfidic sediments. These sources differ in 34S/32S isotope ratios (δ34S values), with phytoplankton-produced DOS tracking marine sulfate (21) and sulfurized DOS mirroring sedimentary porewater sulfide (â¼0 to -10). We measured the δ34S values of solid-phase extracted (SPE) DOM from marine water columns and porewater from sulfidic sediments. Marine DOMSPE δ34S values ranged from 14.9 to 19.9 and C:S ratios from 153 to 303, with lower δ34S values corresponding to higher C:S ratios. Marine DOMSPE samples showed consistent trends with depth: δ34S values decreased, C:S ratios increased, and δ13C values were constant. Porewater DOMSPE was 34S-depleted (â¼-0.6) and sulfur-rich (C:S â¼37) compared with water column samples. We interpret these trends as reflecting at most 20% (and on average â¼8%) contribution of abiotic sulfurized sources to marine DOSSPE and conclude that sulfurized porewater is not a main component of oceanic DOS and DOM. We hypothesize that heterogeneity in δ34S values and C:S ratios reflects the combination of sulfurized porewater inputs and preferential microbial scavenging of sulfur relative to carbon without isotope fractionation. Our findings strengthen links between oceanic sulfur and carbon cycling, supporting a realization that organic sulfur, not just sulfate, is important to marine biogeochemistry.
Subject(s)
Dissolved Organic Matter , Sulfur , Carbon , Nitrogen/analysis , Phosphorus , Phytoplankton , Sulfates/analysis , Sulfides , Sulfur Isotopes , WaterABSTRACT
The mining of ionic rare earth elements in Ganzhou left large area of barren tailings with severe vegetation destruction in pressing needs of remediation. However, the remediating effects of soil additives combined with revegetation on the preservation of nutrients in the tailings and microbial communities were rarely studied. For this purpose, pilot experiments were implemented in a field, with the control group (CK) only cultivating plants without adding materials, and three treatments including peanut straw biochar composite (T1), phosphorusmagnesium composite (T2) and modified zeolite composite (T3) along with the cultivation of Medicago sativa L., Paspalum vaginatum Sw. and Lolium perenne L. Soil pH and organic matter in CK significantly decreased from 4.90 to 4.17 and from 6.62 g/kg to 3.87 g/kg after six months, respectively (p ≤ 0.05), while all the treatments could effectively buffer soil acidification (over 5.74) and delay the loss of soil organic matter. Soil cation exchange capacity was still below the detection limit in all the groups except T2. The results of rainfall runoff monitoring indicated that compared with CK, only T2 could significantly reduce the runoff loss of soil NO3- and SO42- by 45.61 %-75.78 % and 64.03 %-76.12 %, respectively (p ≤ 0.05). Compared with CK, the bacterial diversity in T2 and T3 significantly increased 21.18 % and 28.15 %, respectively (p ≤ 0.05), while T1 didn't change the bacterial or fungal diversity (p > 0.05). Co-occurrence network analysis showed that compared with CK, the whole microbial communities interacted more closely in the three treatments. Functional prediction of the microbial communities revealed all the treatments were dominated by carbon transforming bacteria and saprotrophic fungi except T2. This study demonstrated that the composite materials combined with revegetation couldn't retain soil nitrogen compounds and sulfate in rare earth tailings in the long term.
Subject(s)
Metals, Rare Earth , Soil Pollutants , Zeolites , Bacteria , Carbon , Magnesium/analysis , Metals, Rare Earth/analysis , Nitrogen Compounds/analysis , Nutrients/analysis , Phosphorus , Soil/chemistry , Soil Microbiology , Soil Pollutants/analysis , Sulfates/analysisABSTRACT
The groundwater resources of mining areas have been in a challenging condition in terms of metal pollution and human health. Therefore, this study investigated the concentration of cobalt (Co), molybdenum (Mo), selenium (Se), tin (Sn), and antimony (Sb) in groundwater samples (wells, qanats, and springs) in a heavily contaminated mining district, South Khorasan, Eastern Iran. Human health risk of the studied metals to target groups was assessed, and water quality of the studied groundwater was investigated in the study area. A total of 367 sampling sites (279 wells, 74 qanats, and 14 springs) in South Khorasan Province were selected to collect the groundwater samples from June to July 2020. Sampling was performed thrice for each sampling point, and hydrochemical parameters were evaluated using a portable multiparameter. Inductively coupled plasma mass spectrometry (ICP-MS) was used to detect the metal concentrations. Results showed an order of Se > Mo > Sn > Co > Sb, and hazard index (HI) demonstrated a warning condition for south of South Khorasan (drinking application), southwest of South Khorasan (Irrigation application), and east and center of South Khorasan (drinking-irrigation application). Hydrochemical parameters showed a classification of "Na + K type" and "Mixed Ca-Mg-Cl type" with an overall group of "Na-Cl-HCO3" for sampled waters. Ficklin-Caboi diagram depicted a classification of "near-neutral low metal," and Schoeller diagram classified studied groundwater as "good" for drinking and irrigation consumptions and "Na-Cl" type based on ion balance diagram. Based on the correlation analysis, positive relationships were recorded among EC, TDS, Cl-, Na+, sulfate, Ca2+, salt, total hardness, Mg2+, ammonia, and K+ measured in the water samples. In essence, arid regions of the world greatly rely upon groundwater resources for drinking and irrigation consumptions, and mining districts with a heavy load of active mines can be a serious threat to the groundwater quality and human health.
Subject(s)
Groundwater , Selenium , Trace Elements , Water Pollutants, Chemical , Humans , Trace Elements/analysis , Selenium/analysis , Antimony/analysis , Molybdenum/analysis , Ammonia/analysis , Iran , Tin/analysis , Environmental Monitoring/methods , Water Pollutants, Chemical/analysis , Groundwater/chemistry , Water Quality , Risk Assessment , Sulfates/analysis , Cobalt/analysisABSTRACT
The Yihe River is an important river in Shandong Province, China. It is a catchment river for the South-to-North Water Diversion Project (SNWDP-ER), providing a variety of benefits and ecosystem services, such as flood and drought regulation, fishery and aquaculture, drinking water sources, and biodiversity conservation. In order to objectively reflect the status and changing trend of water environmental quality of the Yihe River, reduce the cost of detection, and improve the efficiency of water quality evaluation, samples were collected at 8 sampling sites in the 220 km main stream of the Yihe River from 2009 to 2019. The spatiotemporal variations of 10 water quality indicators were analyzed, including pH, water temperature (WT), dissolved oxygen (DO), 5-day biochemical oxygen demand (BOD5), chemical oxygen demand (COD), total phosphorus (TP), ammonia nitrogen (NH3-N), nitrate (NO3-N), fluoride (F-), and sulphate (SO42-). The water quality index (WQI) was used to evaluate the spatiotemporal water quality changes, and the minimum WQI (WQImin) model consisting of five key indicators, i.e., NH3-N, BOD5, DO, SO42-, and WT, was built by using stepwise multiple linear regression analysis. The results indicated that the water quality indicators in the Yihe River showed significant spatiotemporal variations. With the exception of the COD and TP, the other water quality indicators conformed to the Class I or II standards of China, indicating that the water quality of the Yihe River was better than most natural water bodies. Seasonally, the WQI was better in the autumn and higher in the upstream area compared to the downstream. The water quality remained at the "good" level. The weighted WQImin model performed well in evaluating water quality, with coefficient of determination (R2), mean square error (MSE), and percentage error (PE) values of 0.903, 3.05, and 1.70%, respectively.
Subject(s)
Drinking Water , Water Pollutants, Chemical , Water Quality , Ecosystem , Drinking Water/analysis , Nitrates/analysis , Fluorides/analysis , Ammonia/analysis , Environmental Monitoring/methods , Water Pollutants, Chemical/analysis , Phosphorus/analysis , Nitrogen/analysis , China , Oxygen/analysis , Sulfates/analysisABSTRACT
Carlsbad Caverns National Park in southeastern New Mexico is adjacent to the Permian Basin, one of the most productive oil and gas regions in the country. The 2019 Carlsbad Caverns Air Quality Study (CarCavAQS) was designed to examine the influence of regional sources, including urban emissions, oil and gas development, wildfires, and soil dust on air quality in the park. Field measurements of aerosols, trace gases, and deposition were conducted from 25 July through 5 September 2019. Here, we focus on observations of fine particles and key trace gas precursors to understand the important contributing species and their sources and associated impacts on haze. Key gases measured included aerosol precursors, nitric acid and ammonia, and oil and gas tracer, methane. High-time resolution (6-min) PM2.5 mass ranged up to 31.8 µg m-3, with an average of 7.67 µg m-3. The main inorganic ion contributors were sulfate (avg 1.3 µg m-3), ammonium (0.30 µg m-3), calcium (Ca2+) (0.22 µg m-3), nitrate (0.16 µg m-3), and sodium (0.057 µg m-3). The WSOC concentration averaged 1.2 µg C m-3. Sharp spikes were observed in Ca2+, consistent with local dust generation and transport. Ion balance analysis and abundant nitric acid suggest PM2.5 nitrate often reflected reaction between nitric acid and sea salt, forming sodium nitrate, and between nitric acid and soil dust containing calcium carbonate, forming calcium nitrate. Sulfate and soil dust are the major contributors to modeled light extinction in the 24-hr average daily IMPROVE observations. Higher time resolution data revealed a maximum 1-hr extinction value of 90 Mm-1 (excluding coarse aerosol) and included periods of significant light extinction from BC as well as sulfate and soil dust. Residence time analysis indicated enrichment of sulfate, BC, and methane during periods of transport from the southeast, the direction of greatest abundance of oil and gas development.Implications: Rapid development of U.S. oil and gas resources raises concerns about potential impacts on air quality in National Parks. Measurements in Carlsbad Caverns National Park provide new insight into impacts of unconventional oil and gas development and other sources on visual air quality in the park. Major contributors to visibility impairment include sulfate, soil dust (often reacted with nitric acid), and black carbon. The worst periods of visibility and highest concentrations of many aerosol components were observed during transport from the southeast, a region of dense Permian Basin oil and gas development.
Subject(s)
Air Pollutants , Nitrates , Nitrates/analysis , Air Pollutants/analysis , Parks, Recreational , Environmental Monitoring , Caves , Nitric Acid/analysis , Aerosols/analysis , Dust/analysis , Gases/analysis , Nitrogen Oxides/analysis , Sulfates/analysis , Soil , Methane/analysis , Particulate Matter/analysisABSTRACT
The typical harm effect of algal bloom sedimentation is to increase sulfides level in surroundings, threatening aquatic organisms and human health; whereas, P inactivating materials containing sulfate are commonly attempted to be used to immobilize reactive P or to flocculate excessive algae in water columns for eutrophication control. In this study, variations in sulfate reduction during algal bloom sedimentation with the addition of sulfate based inactivating materials was comprehensively assessed based on using Al2(SO4)3 with comparison to AlCl3. The results showed that addition of Al2(SO4)3 had more substantial effect on overlying water and sediment properties compared to those of ACl3. Al2(SO4)3 can enhance sulfate reduction, resulting in temporary increase of sulfides (p < 0.01) and quick decrease of various Fe (p < 0.01) in overlying water and then promoting the formation of FeS and FeS2 (determined by EXAFS analysis) in sediments. Most importantly, the increased sulfides, as well as the physical barrier on sediment formed due to Al2(SO4)3 addition, enhanced the transformation of sulfides to odorous contaminants, increasing odorous contaminants (especially methyl thiols) production by approximately one order of magnitude in overlying water. Furthermore, the increased sulfides facilitated to the enrichment of microorganisms related to S cycles (Thiobacillu with relative abundance of 23.8%) and even promoted to enrich bacterial genus potentially with pathogenicity (Treponema) in sediments. The impacts of sulfate tended to be regulated by algae concentration; however, careful management was recommended for sulfate based inactivating materials application to control eutrophication with algal blooms.
Subject(s)
Sulfates , Water Pollutants, Chemical , China , Eutrophication , Geologic Sediments/analysis , Humans , Lakes/analysis , Phosphorus/analysis , Sulfates/analysis , Sulfides/analysis , Sulfur Oxides , Water/analysis , Water Pollutants, Chemical/analysisABSTRACT
Decoupling natural and anthropogenic impacts on the subsurface environment can be difficult, particularly when it has been subject to a wide range of influences over time and space. In this work we show how the use of hydrogeochemical plotting tools, time-series analysis of key contaminants of concern, and targeted isotopic analysis can be used to better understand the contamination sources/processes in a complex environment - a Wastewater Treatment Plant (WWTP) located on coastal acid sulfate soils (ASS). Analysis of soil profiles for potential oxidisable sulfur, acid neutralising capacity (ANC), and pHfox along with groundwater chemistry, revealed that oxidation of pyritic sediments, initially deposited during the mid-Holocene, have led to significant pH declines and the secondary mobilisation of metals into the groundwater environment. This is further complicated by historic anthropogenic inputs associated with the WWTP (e.g., effluent leakages) and the surrounding agricultural land uses. There is distinct separation between spatial and temporal trends in the nutrient and heavy metals data in groundwater, suggesting these reflect different contaminant sources and/or processes. Isotopic data indicate nutrients are largely derived from the WWTP, whereas time-series analysis of key contaminants of concern and hydrogeochemical plotting tools indicate metals are largely derived from the secondary mobilisation of ASS due to acidity generated during sulfide oxidation. This work highlights the importance of understanding the hydrogeological environment and need for careful planning and ongoing management of WWTP sites, particularly those constructed on potential acid sulfate soils (PASS), which, if disturbed or exposed, can lead to impacts beyond the area of ASS via groundwater discharge to nearby surface water bodies (in this case the site is adjacent to a Ramsar-listed wetland). The outcomes of this work have significant global application in the identification, assessment, and control of ASS, the practice of contaminant source attribution, and the siting and design of future WWTPs, which will continue to be sited in coastal areas to meet population needs.
Subject(s)
Groundwater , Water Pollutants, Chemical , Water Purification , Environmental Monitoring , Soil , Sulfates/analysis , Water Pollutants, Chemical/analysisABSTRACT
3D porous hydroxyapatite (HA) has been reinforced by zirconia (ZrO2) coating and impregnation with a combination of platelet rich plasma (PRP) as a source of growth factors (GFs) and Heparin sulfate (HS) to sustain the release of GFs. Adipose mesenchymal stem cells (ADMSCs) were characterized by flow cytometry for CD (cluster of differentiation) 44, CD105, CD106, CD34 and CD144, along with checking the multipotency by differentiation into the adipocytes and osteoblasts. Then, they were cultured on the scaffold treated with and without osteogenic media on days 7, 14 and 21. Electron micrograph and PKH staining show that the ADMSCs have a fusiform phenotype in the absence of osteogenic induction. Cell viability assay shows a higher number of the viable cells on the PRP-containing scaffolds than PRP-free scaffolds on day 7. Colorimetric evaluation, quantitative RT-PCR and immunocytochemistry demonstrate that PRP and HS significantly elevate the alkaline phosphatase enzyme activity and also accelerate the production of both early and mid-osteogenic markers, including collagen I and osteopontin expression with and without osteogenic conditions. The PRP-HS also accelerates the expression of the late osteogenic marker, osteocalcin, in both mRNA and protein level expression with a peak on day 21. In conclusion, supplementation of HA/ZrO2 with PRP/HS has a synergistic impact on the ADMSCs, even in the absence of chemical induction. It seems that HA/ZrO2/PRP/HS scaffold provides a higher osteoconductive microenvironment for stem cell differentiation to osteoblasts.
Subject(s)
Mesenchymal Stem Cells , Platelet-Rich Plasma , Durapatite/pharmacology , Durapatite/analysis , Durapatite/chemistry , Heparin , Sulfates/analysis , Sulfates/metabolism , Osteogenesis , Platelet-Rich Plasma/metabolism , Osteoblasts , Cell Differentiation , Cells, CulturedABSTRACT
Reservoir souring, which is the production of H2S mainly by sulfate-reducing microorganisms (SRM) in oil reservoirs, has been a long-standing issue for the oil industry. While biocides have been frequently applied to control biogenic souring, the effects of biocide treatment are usually temporary, and biocides eventually fail. The reasons for biocide failure and the long-term response of the microbial community remain poorly understood. In this study, one-time biocide treatments with glutaraldehyde (GA) and an aldehyde-releasing biocide (ARB) at low (100 ppm) and high (750 ppm) doses were individually applied to a complex SRM community, followed by 1 year of monitoring of the chemical responses and the microbial community succession. The chemical results showed that souring control failed after 7 days at a dose of 100 ppm regardless of the biocide type and lasting souring control for the entire 1-year period was achieved only with ARB at 750 ppm. Microbial community analyses suggested that the high-dose biocide treatments resulted in 1 order of magnitude lower average total microbial abundance and average SRM abundance, compared to the low-dose treatments. The recurrence of souring was associated with reduction of alpha diversity and with long-term microbial community structure changes; therefore, monitoring changes in microbial community metrics may provide early warnings of the failure of a biocide-based souring control program in the field. Furthermore, spore-forming sulfate reducers (Desulfotomaculum and Desulfurispora) were enriched and became dominant in both GA-treated groups, which could cause challenges for the design of long-lasting remedial souring control strategies. IMPORTANCE Reservoir souring is a problem for the oil and gas industry, because H2S corrodes the steel infrastructure, downgrades oil quality, and poses substantial risks to field personnel and the environment. Biocides have been widely applied to remedy souring, but the long-term performance of biocide treatments is hard to predict or to optimize due to limited understanding of the microbial ecology affected by biocide treatment. This study investigates the long-term biocide performance and associated changes in the abundance, diversity, and structure of the souring microbial community, thus advancing the knowledge toward a deeper understanding of the microbial ecology of biocide-treated systems and contributing to the improvement of current biocide-based souring control practices. The study showcases the potential application of incorporating microbial community analyses to forecast souring, and it highlights the long-term consequences of biocide treatment in the microbial communities, with relevance to both operators and regulators.
Subject(s)
Bacteria/drug effects , Disinfectants/pharmacology , Microbiota/drug effects , Acids/analysis , Acids/metabolism , Bacteria/classification , Bacteria/isolation & purification , Bacteria/metabolism , Oil and Gas Fields/chemistry , Oil and Gas Fields/microbiology , Oxidation-Reduction , Sulfates/analysis , Sulfates/metabolism , Time FactorsABSTRACT
Ultrasound-assisted water extraction was optimized to recover gelling biopolymers and antioxidant compounds from Mastocarpus stellatus. A set of experiments following a Box-Behnken design was proposed to study the influence of extraction time, solid liquid ratio, and ultrasound amplitude on the yield, sulfate content, and thermo-rheological properties (viscoelasticity and gelling temperature) of the carrageenan fraction, as well as the composition (protein and phenolic content) and antiradical capacity of the soluble extracts. Operating at 80 °C and 80 kHz, the models predicted a compromise optimum extraction conditions at ~35 min, solid liquid ratio of ~2 g/100 g, and ultrasound amplitude of ~79%. Under these conditions, 40.3% carrageenan yield was attained and this product presented 46% sulfate and good mechanical properties, a viscoelastic modulus of 741.4 Pa, with the lowest gelling temperatures of 39.4 °C. The carrageenans also exhibited promising antiproliferative properties on selected human cancer cellular lines, A-549, A-2780, HeLa 229, and HT-29 with EC50 under 51.9 µg/mL. The dried soluble extract contained 20.4 mg protein/g, 11.3 mg gallic acid eq/g, and the antiradical potency was equivalent to 59 mg Trolox/g.
Subject(s)
Carrageenan/isolation & purification , Carrageenan/pharmacology , Cell Proliferation/drug effects , Plant Extracts/isolation & purification , Plant Extracts/pharmacology , Rhodophyta/chemistry , Antioxidants/chemistry , Carrageenan/analysis , Carrageenan/chemistry , Cell Line, Tumor , Gels , Humans , Mechanical Phenomena , Phenols/chemistry , Plant Extracts/analysis , Plant Extracts/chemistry , Rheology , Sulfates/analysis , Temperature , Ultrasonic Waves , Viscosity , Water/chemistryABSTRACT
There is still a lack of understanding of H2S formation in agricultural waste, which leads to poor odour prevention and control. Microbial sulfate reduction is a major process contributing to sulfide formation in natural and technogenic environments with high sulfate and low oxygen concentration. Agricultural waste can be considered a low-sulfate system with no obvious input of oxidised sulfur compounds. The purpose of this study was to characterise a microbial community participating in H2S production and estimate the microbial sulfate reduction rate (SRR) in manure slurry from a large-scale swine finishing facility in Western Siberia. In a series of manure slurry microcosms, we identified bacterial consortia by 16S rRNA gene profiling and metagenomic analysis and revealed that sulfate-reducing Desulfovibrio were key players responsible for H2S production. The SRR measured with radioactive sulfate in manure slurry was high and comprised 7.25 nmol S cm-3 day-1. Gypsum may be used as a solid-phase electron acceptor for sulfate reduction. Another plausible source of sulfate is a swine diet, which often contains supplements in the form of sulfates, including lysine sulfate. Low-sulfur diet, manure treatment with iron salts, and avoiding gypsum bedding are possible ways to mitigate H2S emissions from swine manure.
Subject(s)
Biotransformation , Desulfovibrio/metabolism , Hydrogen Sulfide/metabolism , Microbiota , Sulfates/metabolism , Animals , Bacteria/metabolism , Environmental Microbiology , Environmental Monitoring , Farms , Hydrogen Sulfide/analysis , Soil/chemistry , Soil Microbiology , Sulfates/analysis , SwineABSTRACT
The objective of this study was to evaluate the relative bioavailability (RB) of manganese (Mn) proteinate compared to Mn sulfate for broilers fed a diet based on corn and soybean meal for 20 d. The diets of 1,350 male Cobb broilers were supplemented with 0, 35, 70, 105, or 140 mg of Mn/kg of feed in the form of Mn sulfate or Mn proteinate. Weight gain, feed intake, feed conversion, bone strength, and Mn concentration in the tibia and liver, as well as the concentration of type I collagen in the tibia, were evaluated. No differences were observed for performance variables (P > 0.05) or for type I collage concentration in broiler tibia (P > 0.05), regardless of the source and level of supplementation used. Relative bioavailability was determined using bone strength values and Mn concentration in the tibia and liver, assuming Mn sulfate as the standard source (100%) by the slope-ratio method. The RB of Mn proteinate based on bone strength was 111%, based on liver Mn concentration was 128%, and based on tibia Mn concentration was 105%. Manganese proteinate was more bioavailable than Mn sulfate; it can be an important source of supplementation to improve bone quality in broilers.
Subject(s)
Chickens , Dietary Supplements , Manganese , Animal Feed/analysis , Animals , Biological Availability , Diet/veterinary , Liver/chemistry , Male , Manganese/pharmacokinetics , Manganese Compounds/analysis , Sulfates/analysis , Tibia/chemistryABSTRACT
Substrate amendments composed of crab shell (CS) waste materials have been shown to significantly improve the longevity and performance of acid mine drainage (AMD) treatment systems containing spent mushroom compost (SMC), yet the development of key microbial populations within these systems has not been investigated. To better understand the effects of CS on microbial dynamics in these systems, clone libraries and real-time quantitative PCR (qPCR) were performed on materials from a laboratory-scale AMD treatment system containing SMC and 0 to 100% CS substrate after receiving a continuous flow of AMD for 148 days (428 pore volumes). The proportion of CS in the substrate positively correlated with the diversity of sulfate-reducing bacteria (SRB) and archaeal clones, but negatively correlated with fungal diversity. CS also impacted microbial community structure, as revealed in Unifrac significance and principal coordinate analysis tests. The column containing 100% CS substrate supported 7 different genera of SRB-the most ever observed in an AMD treatment system. Moreover, the copy numbers of functional genes representing fermenters, sulfate reducers, and chitin degraders increased with increasing proportions of CS. These observations agree well with the chemical performance data, further validating that by supporting more abundant key microbial groups, chitinous substrates may provide benefits for improving both the longevity and performance of AMD treatment systems, and may provide similar benefits for the treatment of other environmental contaminants that are amenable to anaerobic bioremediation.Key points⢠Crab shell improves the longevity and performance of acid mine drainage treatment.⢠The diversity of sulfate-reducing bacteria is enhanced with crab shell amendments.⢠Crab shell supports more abundant key microbial groups than spent mushroom compost.
Subject(s)
Brachyura , Acids , Animals , Biodegradation, Environmental , Mining , Sulfates/analysisABSTRACT
Mountaintop removal coal mining is the predominant form of surface mining in the Appalachian Region of the United States and leads to elevated levels of chemical constituents in streams draining mined watersheds. This data set contains measurements of water chemistry in the mountaintop mined landscape of Central Appalachia. These data were collected to determine the accumulation and transport of mercury (Hg) and selenium (Se) across environmental compartments in mountaintop mining-impacted waters as well as the impact of mountaintop mining on the aquatic-terrestrial subsidy. Samples were collected in summer 2017 and spring/summer 2018. Data predominantly include Se, THg, and methylmercury (MeHg) in the following environmental compartments at sites impacted and unimpacted by mountaintop removal coal mining: water, sediment, biofilm, larval cranefly, adult aquatic insects, and spiders. Additional water parameters that have been found to vary across a gradient of mountaintop mining impact (total organic carbon [TOC], total nitrogen [TN], sulfate [SO4 ], nitrate [NO3 ], chloride [Cl], pH, sodium [Na], potassium [K], magnesium [Mg], calcium [Ca], manganese [Mn], sulfur [S], specific conductance) were also measured. The majority of sites represented are headwater streams, although some settling ponds below valley fills are included. This data set also includes taxonomic characterization of the adult aquatic insect community as well as measurements of spider density at a subset of sites. There are no copyright restrictions; please cite this data paper when the data are used in publications.
Subject(s)
Coal Mining , Selenium , Water Pollutants, Chemical , Animals , Appalachian Region , Environmental Monitoring , Rivers , Selenium/analysis , Sulfates/analysis , Water Pollutants, Chemical/analysisABSTRACT
The processing of sediment to accurately characterize the spatially-resolved depth profiles of geophysical and geochemical properties along with signatures of microbial density and activity remains a challenge especially in complex contaminated areas. This study processed cores from two sediment boreholes from background and contaminated core sediments and surrounding groundwater. Fresh core sediments were compared by depth to capture the changes in sediment structure, sediment minerals, biomass, and pore water geochemistry in terms of major and trace elements including pollutants, cations, anions, and organic acids. Soil porewater samples were matched to groundwater level, flow rate, and preferential flows and compared to homogenized groundwater-only samples from neighboring monitoring wells. Groundwater analysis of nearby wells only revealed high sulfate and nitrate concentrations while the same analysis using sediment pore water samples with depth was able to suggest areas high in sulfate- and nitrate-reducing bacteria based on their decreased concentration and production of reduced by-products that could not be seen in the groundwater samples. Positive correlations among porewater content, total organic carbon, trace metals and clay minerals revealed a more complicated relationship among contaminant, sediment texture, groundwater table, and biomass. The fluctuating capillary interface had high concentrations of Fe and Mn-oxides combined with trace elements including U, Th, Sr, Ba, Cu, and Co. This suggests the mobility of potentially hazardous elements, sediment structure, and biogeochemical factors are all linked together to impact microbial communities, emphasizing that solid interfaces play an important role in determining the abundance of bacteria in the sediments.