ABSTRACT
BACKGROUND: Trypanosomiasis is one of the neglected tropical diseases of both humans and animals which decreases their productivity and causes death in the worst scenario. Unavailability of vaccines, the low therapeutic index of trypanocidal drugs, and the development of resistance lead to the need for research focused on developing alternative treatment options especially from medicinal plants. The present study was aimed to investigate antitrypanosomal activities of leaves of Cymbopogon citratus and seeds of Lepidium sativum in in-vivo mice model. METHODS: The plant extracts were prepared by maceration using 80% methanol and reconstituted with 10% dimethyl sulfoxide (DMSO) to have the desired concentration. The test doses were adjusted to 100, 200 and 400 mg/kg based on the toxicity profile. The plants extracts were administered to the respective groups of mice after the 12th day of field isolate T. congolense inoculation for seven consecutive days. The level of parasitemia, bodyweight, packed cell volume (PCV), and differential white blood cell counts were measured. RESULTS: The in -vivo test results revealed that both plant extracts had dose-dependent antitrypanosomal activity. Both crude extracts showed a significant reduction in parasite load (P < 0.05), increased or prevent the fall of PCV value (P < 0.05), decreased lymphocytosis and increased neutrophil counts (p < 0.05) and improved bodyweight but significant bodyweight increment (P < 0.05) was observed only in C. citratus treated mice compared to the negative and positive controls. CONCLUSION: The present study concluded that the crude extracts of leaves of C. citratus and seeds of L. sativum had antitrypanosomal effects. Both plants extracts reduced parasitemia level, prevented anemia and improved bodyweight of treated mice. Comparative results from all tested parameters showed that the best activities were observed with C. citratus treated groups of mice.
Subject(s)
Plant Extracts/pharmacology , Trypanocidal Agents/pharmacology , Trypanosoma congolense/drug effects , Animals , Cymbopogon , Ethiopia , Female , Lepidium sativum , Male , Mice , Parasitemia/drug therapy , Plant Leaves , SeedsABSTRACT
A chemical examination of an extract from the aerial part of Oxytropis lanata led to the isolation and identification of 36 compounds, including saponins, isoflavonoids, oxazoles, and glycosides. The three among them were previously unreported oleanane-type saponins. In trypanocidal screening, 5,7,4'-trihydroxyisoflavone showed inhibitory activity against Trypanosoma congolense (IC50 = 10.5 µM), the causative agent of African trypanosomosis in animals; this activity was similar to that of active compounds from the roots of this plant. O. lanata is known to be a traditional medicinal plant in Mongolia for the treatment of inflammatory diseases. The anti-hyaluronidase effect of saponins 3, 5, 8, and 9, (IC50 = 0.15-0.22 mM) was stronger than that of sodium cromoglicate, which was used as a reference drug (IC50 = 0.37 mM). The chemical structures of the new saponins were determined based on HRFABMS, 1H and 13C NMR, 1H-1H COSY, HMQC, HMBC, and ROESY spectroscopic data along with chemical procedures.
Subject(s)
Antiprotozoal Agents/pharmacology , Hyaluronoglucosaminidase/antagonists & inhibitors , Oxytropis/chemistry , Saponins/pharmacology , Antiprotozoal Agents/isolation & purification , Isoflavones/isolation & purification , Isoflavones/pharmacology , Molecular Structure , Mongolia , Oleanolic Acid/analogs & derivatives , Oleanolic Acid/isolation & purification , Oleanolic Acid/pharmacology , Phytochemicals/isolation & purification , Phytochemicals/pharmacology , Plant Components, Aerial/chemistry , Plants, Medicinal/chemistry , Saponins/isolation & purification , Trypanosoma congolense/drug effectsABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: Pastoralists in Nigeria mix barks of Anogeissus leiocarpus (AL) Khaya senegalensis (KS) and potash (Pt) to treat animal African trypanosomosis. AIM: To evaluate antitrypanosomal potential of A. leiocarpus, K. senegalensis and potash for insights into the traditional claim of antitrypanosomal combination therapy (ATCT). MATERIALS AND METHODS: Fifty microliter each of six different concentrations of AL, KS, Pt, AL + KS, AL + KS + Pt and diminazene aceturate (DA, positive control) was incubated with 50 µL of parasite-laden blood containing 108Trypanosoma congolense cells in a 96-well microtitre plate. Negative control wells were devoid of the extracts and drug but supplemented with phosphate-buffered saline (PBS). Efficacy of treatment was observed at 1 h interval for complete immobilisation or reduced motility of the parasites. Each incubated mixture was inoculated into mouse at the point of complete immobilisation of parasite motility or at the end of 6-h observation period for concentrations that did not immobilise the parasites completely. For in vivo assessment, thirty-five parasitaemic rats were randomly allocated into seven groups of 5 rats each. Each rat in groups I-V was treated with 500 mg/kg of AL, KS, Pt, AL + KS and AL + KS + Pt, respectively, for 7 days. Rats in groups VI and VII were treated with diminazene aceturate 3.5 mg/kg once and PBS 2 mL/kg (7 days), which served as positive and negative controls, respectively. Daily monitoring of parasitaemia through the tail vein, packed cell volume and malondialdehyde were used to assess efficacy of the treatments. RESULTS: The AL + KS + Pt group significantly (p < 0.05) and dose-dependently reduced parasite motility and completely immobilized the parasites at 10, 5 and 2.5 µg/µL with an IC50 of 9.1×10-4 µg/µL. All the mice with conditions that produced complete cessation of parasite motility did not develop parasitaemia within one month of observation. The AL + KS group significantly (p < 0.05) lowered the level of parasitaemia and MDA, and significantly (p < 0.05) maintained higher PCV than PBS group. CONCLUSION: The combination of A. leiocarpus and K. senegalensis showed better antitrypanosomal effects than single drug treatment and offers prospects for ATCT. Our findings support ethnopharmacological use of combined barks of A. leiocarpus and K. senegalensis by pastoralist in the treatment of animal African trypanosomosis in Nigeria.
Subject(s)
Combretaceae/chemistry , Complex Mixtures/chemistry , Meliaceae/chemistry , Trypanocidal Agents/administration & dosage , Trypanosomiasis, African/drug therapy , Animals , Disease Models, Animal , Dose-Response Relationship, Drug , Drug Therapy, Combination , Female , Male , Mice , Nigeria , Parasitemia/drug therapy , Parasitemia/parasitology , Plant Extracts/administration & dosage , Plant Extracts/isolation & purification , Plant Extracts/pharmacology , Rats , Rats, Wistar , Trypanocidal Agents/isolation & purification , Trypanocidal Agents/pharmacology , Trypanosoma congolense/drug effects , Trypanosomiasis, African/parasitologyABSTRACT
Vegetable oils are frequently used as solvents for lipophilic materials; accordingly, the effects of their components should be considered in animal experiments. In this study, the effects of various vegetable oils on the course of Trypanosoma congolense infection were examined in mice. C57BL/6J mice were orally administered four kinds of oils (i.e., coconut oil, olive oil, high oleic safflower oil, and high linoleic safflower oil) with different fatty acid compositions and infected with T. congolense IL-3000. Oil-treated mice infected with T. congolense showed significantly higher survival rates and lower parasitemia than those of control mice. Notably, coconut oil, which mainly consists of saturated fatty acids, delayed the development of parasitemia at the early stage of infection. These results indicated that vegetable oil intake could affect T. congolense infection in mice. These findings have important practical implications; for example, they suggest the potential effectiveness of vegetable oils as a part of the regular animal diet for controlling tropical diseases and indicate that vegetable oils are not suitable solvents for studies of the efficacy of lipophilic agents against T. congolense.
Subject(s)
Plant Oils/administration & dosage , Trypanosoma congolense/drug effects , Trypanosomiasis, African/diet therapy , Animals , Body Weight/drug effects , Coconut Oil/administration & dosage , Coconut Oil/chemistry , Coconut Oil/pharmacology , Energy Intake/drug effects , Linoleic Acid/analysis , Male , Mice , Mice, Inbred C57BL , Oleic Acid/analysis , Olive Oil/administration & dosage , Olive Oil/chemistry , Olive Oil/pharmacology , Parasitemia/prevention & control , Plant Oils/classification , Plant Oils/pharmacology , Plant Oils/therapeutic use , Safflower Oil/administration & dosage , Safflower Oil/chemistry , Safflower Oil/pharmacology , Trypanosomiasis, African/prevention & controlABSTRACT
Cell viability assays using multi-well cell culture plates are frequently used for in vitro drug screening. We herein describe an ATP-based luciferase viability assay for animal African trypanosomes using a 96-well plate. This assay could be further applied to the screening of novel compounds for the treatment of animal African trypanosomiasis.
Subject(s)
Cell Survival/drug effects , Trypanocidal Agents/pharmacology , Trypanosoma brucei brucei/drug effects , Trypanosoma congolense/drug effects , Trypanosomiasis, African/veterinary , Adenosine Triphosphate/analysis , Adenosine Triphosphate/metabolism , Animals , Diminazene/analogs & derivatives , Diminazene/pharmacology , Drug Evaluation, Preclinical/instrumentation , Drug Evaluation, Preclinical/methods , Humans , Inhibitory Concentration 50 , Luciferases/metabolism , Pentamidine/pharmacology , Trypanosomiasis, African/drug therapyABSTRACT
BACKGROUND: The search for novel antitrypanosomal agents had previously led to the isolation of ellagic acid as a bioactive antitrypanosomal compound using in vitro studies. However, it is not known whether this compound will elicit antitrypanosomal activity in in vivo condition which is usually the next step in the drug discovery process. PURPOSE: Herein, we investigated the in vivo activity of ellagic acid against bloodstream form of Trypanosoma congolense and its ameliorative effects on trypanosome-induced anemia and organ damage as well as inhibitory effects on trypanosomal sialidase. METHODS: Rats were infected with T. congolense and were treated with 100 and 200mg/kg body weight (BW) of ellagic acid for fourteen days. The levels of parasitemia, packed cell volume and biochemical parameters were measured. Subsequently, T. congolense sialidase was partially purified on DEAE cellulose column and the mode of inhibition of ellagic acid on the T. congolense sialidase determined. Molecular docking study was also conducted to determine the mode of interaction of the ellagic acid to the catalytic domain of T. rangeli sialidase. RESULTS: At a dose of 100 and 200mg/kg (BW), ellagic acid demonstrated significant (P < 0.05) trypanosuppressive effect for most of the 24 days experimental period. Further, the ellagic acid significantly (P < 0.05) ameliorated the trypanosome-induced anemia, hepatic and renal damages as well as hepatomegaly, splenomegaly and renal hypertrophy. The trypanosome-associated free serum sialic acid upsurge alongside the accompanied membrane bound sialic acid reduction were also significantly (P < 0.05) prevented by the ellagic acid treatment. The T. congolense sialidase was purified to a fold of 6.6 with a yield of 83.8%. The enzyme had a KM and Vmax of 70.12mg/ml and 0.04µmol/min respectively, and was inhibited in a non-competitive pattern by ellagic acid with an inhibition binding constant of 1986.75µM. However, in molecular docking study, ellagic acid formed hydrogen bonding interaction with major residues R39, R318, and W124 at the active site of T. rangeli sialidase with a predicted binding free energy of -25.584kcal/mol. CONCLUSION: We concluded that ellagic acid possesses trypanosuppressive effects and could ameliorate the trypanosome-induced pathological alterations.
Subject(s)
Ellagic Acid/pharmacology , Neuraminidase/antagonists & inhibitors , Trypanocidal Agents/pharmacology , Trypanosoma congolense/drug effects , Trypanosomiasis, African/drug therapy , Animals , Computer Simulation , Enzyme Inhibitors/pharmacology , Hematocrit , Hydrogen Bonding , Molecular Docking Simulation , Neuraminidase/chemistry , Neuraminidase/metabolism , Parasitemia/drug therapy , Rats, Wistar , Trypanocidal Agents/chemistry , Trypanosoma congolense/metabolismABSTRACT
BACKGROUND: The search for alternative trypanocidal compounds which can be available at affordable price is of paramount importance for control of trypanosomosis in human and animals. The current study evaluates the in vivo activity of ethanolic stem bark extracts on Trypanosoma congolense and selected immunological components in an inbred Swiss albino mouse model. METHODS: Groups of mice infected with T. congolense were treated with the stem bark extracts at a rate of 1000 mg/kg, 1500 mg/kg, and 2000 mg/kg, twice a day in one set and thrice a day in another setting for three days consecutively. Negative (infected and untreated) and positive (infected treated with diminazene diaceturate at 3.5 mg/kg) control groups were used. Levels of parasitaemia were monitored daily for the first 10 days and thereafter 2-3 times per week to the end of experiment. In the other setting, uninfected mice, randomized in groups were treated with the extract but categorized as: thorough mixed extract (TME) and supernatant extract (SE) each at 500 mg/kg and 1500 mg/kg, in 8 hourly intervals respectively for three days consecutively. Control group was administered with phosphate buffered saline with glucose at 0.1 ml/10 g in a similar manner as for the extract. Whole blood and spleen were taken 24 h after the last treatment for hematological and histopathological analysis. RESULTS: The groups that received the extracts at 8 hourly intervals drastically reduced the parasitaemia. The higher dose of SE significantly reduced the percentage of lymphocytes (P < 0.05). Both high and low dose of TME significantly reduced lymphocytes percent (P < 0.05) while percent of neutrophils and monocytes increased significantly (P < 0.05). Histopathological changes of the spleen in the mice treated with higher concentrations of the extract of C. swynnertonii were suggestive of lymphocytes toxicity. CONCLUSION: The current study has provided evidence that, in vivo trypanocidal activity of ethanolic bark extracts of C. swynnertonii is probably affected by its negative effect on humoral mediated immune response. Further studies are recommended to determine its potential as an alternative source of lead compounds for trypanocidal drug discovery.
Subject(s)
Commiphora/chemistry , Plant Extracts/administration & dosage , Trypanocidal Agents/administration & dosage , Trypanosoma congolense/drug effects , Trypanosomiasis, African/drug therapy , Animals , Disease Models, Animal , Humans , Male , Mice , Phytotherapy , Plant Bark/chemistry , Plant Extracts/isolation & purification , Treatment Outcome , Trypanocidal Agents/isolation & purification , Trypanosoma congolense/physiology , Trypanosomiasis, African/parasitologyABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: Leaves from the plant species studied herein are traditionally used in northern Nigeria against various protozoan infections. However, none of these herbal preparations have been standardized, nor have their toxicity to mammalian cells been investigated. In search of improved and non-toxic active antiprotozoal principles that are not cross-resistant with current anti-parasitics, we here report the results of the in vitro screening of extracts from seven selected medicinal plant species (Centrosema pubescens, Moringa oleifera, Tridax procumbens, Polyalthia longifolia, Newbouldia laevis, Eucalyptus maculate, Jathropha tanjorensis), used traditionally to treat kinetoplastid infections in Nigeria, and the isolation of their bioactive principles. AIM OF THE STUDY: To investigate the efficacies of medicinal plant extracts, and of compounds isolated therefrom, against kinetoplastid parasites, assess cross-resistance to existing chemotherapy, and assay their toxicity against mammalian cells in vitro. MATERIAL AND METHODS: Plants were extracted with hexane, ethyl acetate and methanol. Active principles were isolated by bioassay-led fractionation, testing for trypanocidal activity, and identified using NMR and mass spectrometry. EC50 values for their activity against wild-type and multi-drug resistant Trypanosoma brucei were obtained using the viability indicator dye resazurin. RESULTS: Seven medicinal plants were evaluated for activity against selected kinetoplastid parasites. The result shows that crude extracts and isolated active compounds from Polyalthia longifolia and Eucalyptus maculata, in particular, display promising activity against drug-sensitive and multi-drug resistant Trypanosoma brucei. The EC50 value of a clerodane (16α-hydroxy-cleroda-3,13(14)-Z-dien-15,16-olide) isolated from Polyalthia longifolia was as low as 0.38µg/mL, while a triterpenoid (3ß,13ß-dihydroxy-urs-11-en-28-oic acid) isolated from Eucalyptus maculata displayed an EC50 of 1.58µg/mL. None of the isolated compounds displayed toxicity towards Human Embryonic Kidney cells at concentrations up to 400µg/mL. In addition, the isolated compounds were active against Leishmania mexicana, as well as against T. congolense. CONCLUSION: We have isolated a clerodane compound from Polyalthia longifolia that shows low toxicity, no cross-resistance with current treatments, and promising activity against both human-infective and veterinary Trypanosoma species.
Subject(s)
Amidines/pharmacology , Arsenicals/pharmacology , Biological Assay/methods , Trypanocidal Agents/pharmacology , Trypanocidal Agents/toxicity , Cell Line , Diterpenes, Clerodane/pharmacology , Diterpenes, Clerodane/toxicity , Drug Resistance , HEK293 Cells , Humans , Leishmania mexicana/drug effects , Medicine, African Traditional , Nigeria , Plant Leaves/chemistry , Trypanosoma brucei brucei/drug effects , Trypanosoma congolense/drug effectsABSTRACT
CONTEXT: Control of African trypanosomiasis relies on chemotherapy, but the development of resistance and the problem of drug residues require research for alternatives. Triterpenes and phenolics, the major constituents of Pleurotus sajor-caju (Fr.) Singer (Pleurotaceae), are reported to be effective against trypanosomiasis. OBJECTIVE: Trypanocidal effect of whole Pleurotus sajor-caju aqueous extract was investigated in vivo against Trypanosoma congolense. MATERIALS AND METHODS: Mice (25-32 g) were divided into seven groups of six animals. Mice in groups A-F received 2.5 × 104 trypanosomes, while group G was uninfected. Extracts (100-250 mg/kg) were administered intraperitoneally for 5 days to groups A-D while diminazine aceturate (group E) and normal saline (group F) served as positive and negative controls, respectively. Parasitemia, survival time, body weight and haematological parameters were monitored for 60 days post-treatment. RESULTS: Parasitemia decreased significantly (p < 0.01) post-treatment with 200 and 250 mg/kg of the extract and became undetectable by day 16 and 12 post-infection, respectively; the ED50 was 221.5 mg/kg. The packed cell volume (PCV) and the weight of mice treated with 250 mg/kg extract were 46.20 ± 2.6% and 32.05 ± 3.63 g, respectively, which is higher than the group treated with diminazine aceturate. The mean survival time of animals in groups D and E was >60 days, while that of group F was <4 days. Differential leucocyte count on day 68 post-infection in groups C, D and E were not significantly different. CONCLUSION: Pleurotus sajor-caju therefore could be a potential source of new trypanocidal drugs.
Subject(s)
Pleurotus/chemistry , Trypanocidal Agents/pharmacology , Trypanosoma congolense/drug effects , Trypanosomiasis, African/drug therapy , Animals , Biomarkers/blood , Body Weight/drug effects , Diminazene/analogs & derivatives , Diminazene/pharmacology , Disease Models, Animal , Dose-Response Relationship, Drug , Mice, Inbred BALB C , Parasitemia/drug therapy , Parasitemia/parasitology , Time Factors , Trypanocidal Agents/isolation & purification , Trypanosomiasis, African/blood , Trypanosomiasis, African/parasitologyABSTRACT
BACKGROUND: African Trypanosomiasis is a neglected tropical disease with a large impact on the livelihood of the rural poor in Sub-Saharan Africa. The available drugs for managing this disease are old, expensive and are facing the problem of drug resistance. Thus, the aim of this study was to evaluate in vivo antitrypanosomal efficacy of aqueous and absolute methanol leaf extracts of Verbascum sinaiticum Benth. against Trypanosoma congolense field isolate. METHODS: Verbascum sinaiticum (Local name 'qetetina') is a biennial plant, and 60-150 cm tall. It is traditionally used to treat wound, stomachache, viral infection, cancer, sunstroke, fever, abdominal colic, diarrhea, hemorrhage, anthrax, and hepatitis. The efficacy of aqueous and absolute methanol leaf extracts of V. sinaiticum was evaluated in a randomized experiment with Swiss albino mice infected with T. congolense field isolate. The extracts were administered at doses of 100, 200 and 400 mg/kg by intraperitoneal injection for seven days at 12 Days Post-Infection (DPI) when the peak parasitaemia level was approximately 10(8) trypanosomes/ml. Parasitaemia, Packed Cell Volume (PCV), mean survival time and change in body weight were used as indices for monitoring the efficacy of the extracts. Diminazene (28 mg/kg) was used as a positive control while 2 % Tween was used as the negative control. Phytochemicals screening were conducted following standard methods. RESULTS: The extracts showed no toxicity effect in Swiss albino mice and had LD50 above 2000 mg/kg. The phytochemicals screened in V. sinaiticum were alkaloids, flavonoids, glycoside, saponins, steroids, phenolic compounds, and tannins. The mice treated with absolute methanol leaf extract of V. sinaiticum at 400 mg/kg dose had significantly lower mean parasitaemia (7.20 ± 0.16) (p < 0.001) as compared to the negative control group (8.82 ± 0.12) on day 14 of treatment. Animals treated with the same dose had significant (p < 0.001) higher PCV value and body weight and as well as the highest mean survival time of 40.20 ± 0.31 days as compared to the negative control at the end of the observation period. CONCLUSION: This study established that Verbascum sinaiticum had trypanocidal activity. The crude extracts have partially eliminated trypanosomes in a dose-dependent manner. The study can be a basis for future in-depth analysis of the biologically active chemicals.
Subject(s)
Plant Extracts/pharmacology , Trypanocidal Agents/pharmacology , Trypanosoma congolense/drug effects , Trypanosomiasis, African/parasitology , Verbascum/chemistry , Animals , Body Weight/drug effects , Disease Models, Animal , Female , Mice , Plant Extracts/chemistry , Plant Extracts/therapeutic use , Plant Extracts/toxicity , Trypanocidal Agents/chemistry , Trypanocidal Agents/therapeutic use , Trypanocidal Agents/toxicity , Trypanosomiasis, African/drug therapyABSTRACT
BACKGROUND: African trypanosomosis is the disease caused by extracellular protozoan parasites of the genus Trypanosoma transmitted by tsetse flies. The current study has evaluated the trypanocidal activity of Commiphora swynnertonii extracts on Trypanosoma congolense. METHODS: The effect of ethanolic stem bark and resinous extracts on motility of T. congolense was evaluated by in vitro study at concentrations of 2 mg/ml and 4 mg/ml. Then, trypanocidal activity was evaluated by drug incubation infectivity test using mice at concentrations of 0.4 mg/ml and 2 mg/ml. In both studies negative (without drug) and positive (diminazene diaceturate) controls were used. RESULTS: The in vitro study showed that, ethanolic stem bark extract of C. swynnertonii at concentration of 4 mg/ml caused complete cessation of motility for T. congolense in 30 min. However, resinous ethanolic extract had delayed effect on cessation of motility of T. congolense observed at 90 and 100 min post-incubation at concentrations of 4 mg/ml and 2 mg/ml respectively. The drug incubation infectivity test study depicted that ethanolic stem bark extract at concentration of 2 mg/ml significantly (p = 0.000) reduced the infectivity of T. congolense in mice. However, it did not vary significantly (P =0.897) with group treated with diminazene diaceturate incubated mixture. CONCLUSION: The current study has provided evidence that, ethanolic stem bark extract of C. swynnertonii possess trypanocidal activity against T. congolense. Based on these findings, further studies are recommended to determine its potential as a lead to trypanocidal drug discovery.
Subject(s)
Commiphora/chemistry , Plant Extracts/pharmacology , Trypanocidal Agents/pharmacology , Trypanosoma congolense/drug effects , Animals , Ethanol , Female , Male , Mice , Plant Extracts/chemistry , Trypanocidal Agents/chemistry , Trypanosomiasis, African/parasitologyABSTRACT
BACKGROUND: African trypanosomiasis affects both humans and livestock in sub-Saharan countries including Ethiopia. Due to limitations to current chemotherapy, there is an urgent need for the development of new, safe, cheap and effective drugs. In the present study, the leaf of Dovyalis abyssinica was tested for its in vivo antitrypanosomal activity against Trypanosoma congolense field isolate on mice. METHODS: The leaf of D. abyssinica was macerated using dichloromethane and methanol. The extracts at doses of 250, 200, 150 and 100 mg/kg body weight were administered intraperitonealy daily for 7 days to mice infected with T. congolense. Following administration, parasitemia, packed cell volume, rectal temperature, body weight and survival time were monitored. RESULTS: Administration of dichloromethane and methanol extracts at 250 and 200 mg/kg reduced (p<0.05) parasitemia and rectal temperature, and improved (p<0.05) PCV, mean body weight, and mean survival time compared to dimethylsulfoxide treatment. CONCLUSION: Crude dichloromethane and methanol leaf extracts of D. abyssinica displayed anti-trypanosomal activity that may serve as lead for the development of effective alternative antitrypanosomal drugs.
Subject(s)
Phytotherapy , Plant Extracts/therapeutic use , Salicaceae , Trypanosoma congolense/drug effects , Trypanosomiasis, African/drug therapy , Animals , Female , Humans , Male , Mice , Parasitemia/parasitology , Parasitemia/prevention & control , Plant Extracts/pharmacology , Plant Leaves , Trypanosomiasis, African/parasitologyABSTRACT
BACKGROUND: Treatment of trypanosomosis is currently facing a number of problems including toxicity of trypanocidal drugs and development of resistance by the parasites. These limitations have prompted the search for alternative active substances (such as of natural origin). The purpose of the study was to investigate the effect of extracts of Moringa stenopetala and Artemisia absinthium on Trypanosoma congolense in mice. METHODS: Swiss white male mice aged 8-12 weeks were divided into six experimental groups of six animals. Water and methanol extracts of the two plants were prepared. T. congolense was isolated from cattle at Ghibe valley (Ethiopia). All experimental mice received approximately 1 x 10(5) trypanosomes in 0.2 ml of blood. Plant extracts were given orally to four groups (2 plant species and two extraction methods) at 400 mg/kg body weight for seven consecutive days. One group remained as distilled water treated control and the other as diminzene aceturate treated control. The effect of the extracts on levels of parasitaemia, body weight, packed cell volume (PCV) and mice survival was monitored for 25 days. RESULTS: All treatments have significantly reduced parasitaemia and helped improve body weight, PCV and survival of mice compared to the water-treated control (P < 0.01 in all cases). These effects were comparable to that with diminazene aceturate. No significant difference was observed in the reduction of parasitaemia between plant extract treatment groups. However, mice with extracts of A. absinthium had significantly higher body weight than those with extracts of M. stenopetala (P < 0.05). CONCLUSIONS: The two plants have antitrypanosomal potential against T. congolense by reducing the levels of parasitaemia, maintaining good PCV and body weight, and prolonging the lives of infected animals.
Subject(s)
Artemisia absinthium/chemistry , Moringa/chemistry , Parasitemia/prevention & control , Plant Extracts/pharmacology , Trypanosoma congolense/drug effects , Animals , Body Weight/drug effects , Cattle , Cattle Diseases/blood , Cattle Diseases/parasitology , Hematocrit , Host-Parasite Interactions/drug effects , Male , Methanol/chemistry , Mice , Parasitemia/parasitology , Phytotherapy , Plant Extracts/administration & dosage , Plant Extracts/chemistry , Survival Analysis , Treatment Outcome , Trypanocidal Agents/administration & dosage , Trypanocidal Agents/chemistry , Trypanocidal Agents/pharmacology , Trypanosoma congolense/physiology , Water/chemistryABSTRACT
The antitrypanosomal activity of the methanol extract of Buchholzia coriacea seed against a field strain of Trypanosoma congolense was investigated using experimentally infected mice of both sexes. Monitoring of parasitaemia was by the rapid matching technique. When parasitaemia was approximately log 7.8 (63 × 10(6) parasites/ml), treatment with graded doses of the extract (250, 500 and 1000 mg/kg) was instituted for 5 consecutive days. Diminazene diaceturate (Dimivet SKM Pharma Pvt. Ltd.) was given at 3.5 mg/kg i.p. to the positive control mice. No significant differences in body weights were observed. The rectal temperatures of infected mice showed fluctuations. The PCV of infected mice were significantly (p < 0.05) lower than those of the uninfected controls. There was no significant difference between the PCV of the extract-treated and untreated animals. Parasitaemia increased steadily in the extract-treated and untreated mice groups till all the animals died. Three days post-treatment with diminazene diaceturate parasitaemia was cleared. Six days later, there was a relapse of infection. By the end of the experiment, a 50 % relapse rate was recorded in the diminazene diaceturate-treated group. The methanol extract of Buchholzia coriacea seeds did not show any antitrypanosomal activity in mice infected with Trypanosoma congolense at the doses tested.
Subject(s)
Capparaceae/chemistry , Diminazene/analogs & derivatives , Plant Extracts/pharmacology , Seeds/chemistry , Trypanocidal Agents/therapeutic use , Trypanosoma congolense/drug effects , Administration, Oral , Animals , Diminazene/pharmacology , Diminazene/therapeutic use , Dose-Response Relationship, Drug , Female , Hematocrit , Male , Methanol , Mice , Parasitemia/drug therapy , Parasitemia/veterinary , Phytotherapy , Plant Extracts/isolation & purification , Trypanocidal Agents/pharmacology , Trypanosoma congolense/isolation & purification , Trypanosomiasis, African/drug therapy , Trypanosomiasis, African/parasitologyABSTRACT
The effects of aqueous extract of Hibiscus sabdariffa calyces on haematology and pathological changes in some selected organs during experimental Trypanosoma congolense infection of rats were investigated. Three groups of rats were intraperitoneally infected with T. congolense (Karu stock). One group was administered with the aqueous extract and another given a solution of vitamin C in drinking water; the remaining infected group was left untreated. Data from these groups were compared with those of two groups of healthy rats, one of which was similarly treated with the aqueous extract. The experiment was terminated three weeks, post-infection (pi). The uninfected and infected rats administered the extract consumed the equivalent of 9.94 mg - and 9.61 mg ascorbic acid / 100g / day during the experiment. Consumption of the extract significantly (p<0.01) retarded the rate of weight gain in both healthy and infected rats; even though the feed-intake was not significantly affected. After two weeks of infection the extract and vitamin C kept the parasitaemia significantly (p<0.01) lower than the untreated infected group. The anaemia in the untreated infected group was significantly (p<0.01) more severe than that of the corresponding extract- or vitamin-treated groups. Trypanosoma congolense infection caused significant (p<0.01) decreases in serum total proteins and albumin; serum and organ ascorbic acid as well as significant (p<0.01) elevation of serum alanine amino transferase levels in untreated rats. Consumption of the extract or vitamin C, however, prevented these disease-induced anomalies in the treated infected rats. Serum creatinine and urea levels were not affected by infection but the extract elevated these parameters significantly (p<0.01) above infection levels. It was concluded that consumption of the extract ameliorated the pathological changes in blood and organs of T. congolense-infected rats.
Subject(s)
Ascorbic Acid/administration & dosage , Hibiscus/chemistry , Plant Extracts/administration & dosage , Trypanosomiasis, African/physiopathology , Anemia/drug therapy , Anemia/etiology , Animals , Ascorbic Acid/metabolism , Creatinine/blood , Hematocrit , Injections, Intraperitoneal , Kidney/pathology , Liver/pathology , Male , Parasitemia/drug therapy , Rats , Rats, Wistar , Trypanosoma congolense/drug effects , Trypanosoma congolense/isolation & purification , Trypanosomiasis, African/drug therapy , Trypanosomiasis, African/microbiology , Trypanosomiasis, African/veterinary , WaterABSTRACT
Only a few drugs are available for chemotherapy of African trypanosomiasis and there is an urgent need for the development of new anti-trypanosomal agents. In this study, the anti-helminthic drug niclosamide was tested for its trypanocidal activity in vitro using culture-adapted bloodstream forms of Trypanosoma brucei brucei and Trypanosoma congolense. The concentrations of niclosamide to reduce the growth rate by 50% and to kill all cells were in the low- and mid micromolar ranges for T. b. brucei and T. congolense, respectively. The very low toxicity of niclosamide for mammals makes the compound interesting for drug development for African trypanosomiasis.
Subject(s)
Niclosamide/pharmacology , Trypanocidal Agents/pharmacology , Trypanosoma brucei brucei/drug effects , Trypanosoma congolense/drug effects , Animals , Anthelmintics/chemistry , Anthelmintics/pharmacology , Anthelmintics/toxicity , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical , HL-60 Cells , Humans , Niclosamide/chemistry , Niclosamide/toxicity , Parasitic Sensitivity Tests , Trypanocidal Agents/toxicity , Trypanosoma brucei brucei/growth & development , Trypanosoma congolense/growth & development , Trypanosomiasis, African/drug therapyABSTRACT
The ethanolic extract of Butyrospermum paradoxum stem bark, commonly used in the traditional treatment of various diseases including animal and human trypanosomosis in north-eastern Nigeria, was tested for toxicity and anti-trypanosomal efficacy in rats infected with Trypanosoma congolense and Trypanosoma brucei. Following intra-peritoneal administration, the extract induced behavioural changes, morbidity and mortality in the rats. The symptoms observed included anorexia, dehydration, depression, prostration, coma and death. These symptoms were noted at high doses (> 800 mg/kg) only. At necropsy, the pathological lesions were mainly congestion and oedema of the lungs, bronchi, bronchioles and the kidney, hepatomegally and focal necrosis of the liver cells. The severity of the symptoms and lesions were dose related. The intra-peritoneal LD50 of the extract was 820 mg/kg. The extract produced anti-trypanosomal effect through the complete suppression or delay in parasite establishment with reduction in the level of parasitaemia and the severity of the attendant disease as well as enhanced survival of the rats infected with Trypanosoma congolense and Trypanosoma brucei. The results suggest that the folkloric medicinal application of the extracts of Butyrospermum paradoxum has a pharmacological basis.
Subject(s)
Plant Extracts/pharmacology , Sapotaceae , Trypanocidal Agents/pharmacology , Trypanosomiasis, African/drug therapy , Animals , Behavior, Animal/drug effects , Dose-Response Relationship, Drug , Female , Hematocrit , Injections, Intraperitoneal , Male , Medicine, African Traditional , Plant Bark , Plant Extracts/administration & dosage , Plant Extracts/adverse effects , Plants, Medicinal , Random Allocation , Rats , Survival Rate , Toxicity Tests, Acute , Trypanocidal Agents/administration & dosage , Trypanocidal Agents/adverse effects , Trypanosoma brucei brucei/drug effects , Trypanosoma congolense/drug effectsABSTRACT
Partially purified azanthraquinone (AQ) extract from Mitracarpus scaber was coupled to bovine transferrin (Tf) using azidophenyl glyoxal (APG). The AQ-APG-Tf conjugate was found to possess an enhanced in vitro trypanocidal activity against Trypanosoma congolense and T. brucei brucei. At low concentrations of 0.39-90 mg/ml, the conjugate diminished the growth of T. congolense and T. b. brucei dose dependently at the logarithmic phase. Both parasites were more sensitive to AQ-APG-Tf than to the free (AQ) extract. Growth inhibition on the parasites by the free extract was observed at 20-200 mg/ml. The total activity of the lysosomal enzyme a-mannosidase was reduced in the T. congolense cells treated with AQ-APG-Tf in a dose related pattern. However, the activity of the mannosidase in the T. b. brucei treated cells is less affected. The AQ-APG-Tf is more effective on a mannosidase than free AQ, eight and four fold for T. congolense and T. b. brucei respectively. The results are discussed as regards the potency of using transferrin as suitable drug carrier in the chemotherapy of Human sleeping sickness.
Subject(s)
Anthraquinones/chemistry , Anthraquinones/pharmacology , Aza Compounds/chemistry , Aza Compounds/pharmacology , Mannosidases/metabolism , Plant Extracts/pharmacology , Rubiaceae/chemistry , Transferrin/pharmacology , Trypanocidal Agents/pharmacology , Trypanosoma brucei brucei/drug effects , Trypanosoma congolense/drug effects , Animals , Dose-Response Relationship, Drug , Glyoxal/analogs & derivatives , Glyoxal/chemistry , In Vitro Techniques , Lysosomes/enzymology , Plant Leaves/chemistry , Transferrin/chemistry , Trypanosoma brucei brucei/enzymology , Trypanosoma brucei brucei/pathogenicity , Trypanosoma congolense/enzymology , Trypanosoma congolense/pathogenicityABSTRACT
The effect of 34 alkaloids of the piperidine, pyridine, tropane, isoquinoline, indole, quinolizidine, quinoline, purine, and steroidal types on the growth of Trypanosoma brucei, T. congolense, and human HL-60 cells was investigated in vitro. Berbamine, berberine, cinchonidine, cinchonine, emetine, ergotamine, quinidine, quinine, and sanguinarine showed trypanocidal activities with ED(50) (50% effective dose) values below 10 microM. Berberine, emetine, and quinidine were the most active compounds found; their ED(50) values and minimum inhibitory concentrations were comparable to those of the antitrypanosomal drugs suramin and diminazene aceturate. However, most of these compounds were also cytotoxic. In the case of emetine, the ratio of cytotoxic/trypanocidal activity was only 3 while for quinidine it was 300 indicating that this alkaloid could be a candidate for further drug development. DNA intercalation in combination with protein biosynthesis inhibition, which is the major mode of action of the active alkaloids, could be responsible for the observed trypanocidal and cytotoxic effects.