Organic-inorganic biohybrid films from wool-keratin/jellyfish-collagen/silica/boron via sol-gel reactions for soft tissue engineering applications.

Therapeutic angiogenesis is pivotal in creating effective tissue-engineered constructs that deliver nutrients and oxygen to surrounding cells. Hence, biomaterials that promote angiogenesis can enhance the efficacy of various medical treatments, encompassing tissue engineering, wound healing, and drug delivery systems. Considering these, we propose a rapid method for producing composite silicon-boron-wool keratin/jellyfish collagen (Si-B-WK/JFC) inorganic-organic biohybrid films using sol-gel reactions. In this approach, reactive tetraethyl orthosilicate and boric acid (pKa ⩾ 9.24) were used as silicon and boron sources, respectively, and a solid-state gel was formed through the condensation reaction of these reactive groups with the keratin/collagen mixture. Once the resulting gel was thoroughly suspended in water, the films were prepared by a casting/solvent evaporation methodology. The fabricated hybrid films were characterized structurally and mechanically. In addition, angiogenic characteristics were determined by the in ovo chick chorioallantoic membrane assay, which revealed an increased vascular network within the Si-B-WK/JFC biohybrid films. In conclusion, it is believed that Si-B-WK/JFC biohybrid films with mechanical and pro-angiogenic properties have the potential to be possessed in soft tissue engineering applications, especially wound healing.

Enhancing wool dyeing with clove bud (Syzygium aromaticum) based natural dye via microwave treatment using a central composite design.

This research investigates the viability of using Syzygium aromaticum (clove) as a natural dye for wool yarn through the application of microwave treatment and optimization using central composite design (CCD). As concerns grow over the environmental impact of synthetic dyes and their detrimental disposal in water bodies, the search for eco-friendly alternatives becomes imperative to revolutionize the textile industry. Microwave-assisted extraction of the colorant from clove powder is explored as an efficient and sustainable method, minimizing solvent usage and energy consumption compared to conventional techniques. To enhance colorfastness properties while eliminating the need for toxic mordants, green alternatives such as Al, Fe, and tannic acid, combined with plant phenolics from red sumac, pomegranate rind, and weld, are employed. According to the analysis of CCD, the higher color strength value 18.1653 was achieved using pH = 3, time = 50 min, temperature = 70 °C, and salt concentration = 1.5 g/100 mL. The optimized dyeing conditions also showed a maximum level of colorfastness properties of 5 for light, 5 for wash, 5 for dry rubbing, and 4 for wet rubbing. The findings from Fourier-transform infrared spectroscopy and scanning electron microscopy analyses provide valuable insights into the chemical and morphological changes induced by microwave treatment and dyeing with clove extract. The results affirm the presence of eugenol as a potential active molecule responsible for the captivating color of clove flower buds, validating its suitability as a natural dye source for wool. This study highlights the promising potential of microwave-assisted extraction and plant-based biomolecules as innovative and environmentally friendly approaches in natural dyeing, paving the way for a more sustainable future in the textile industry. Embracing these eco-friendly practices allows the textile sector to reduce its ecological footprint and contribute to a cleaner and greener environment. Further research and implementation of these techniques can foster a more harmonious coexistence with nature, ensuring a healthier ecosystem for all.

Anar Phali (Opuntia ficus) juice extract as a novel pollution-free source of natural betalain dye for wool yarn.

Green products such as plant pigments in all filed are gaining fame globally due to their excellent ayurvedic and biological characteristics. In this study, microwave rays have been employed for the isolation of colorants from Anar Phali while bio-mordant have been included to get color-fast shades. The colorant was isolated in an acidic medium before and after microwave rays for 2 min. For getting darker shades with different tints, sustainable chemical and plant-based extracts as bio-mordant have been employed before and after bio coloration of wool yarn at given conditions. CIE Lab system computed in Colori-spectrophotometer (CS-410) was used to observe the change in color depth and tonal variation of dyed fabrics, and ISO standard methods have been employed to rate the colorfastness to light, washing, and rubbing at grey scale. It is concluded that microwave rays have an excellent sustainable efficacy to isolate colorant from Anar Phali powder for wool dyeing, whereas the addition of bio-mordants has made the process more sustainable and eco-friendly.

Laccase-assisted colouration of wool fabric using green tea extract for imparting antioxidant, antibacterial, and UV protection activities.

The demand for natural dyes for imparting sustainable dyeing effects to textiles is increasing. Metal mordants generate an unstainable impact in the natural dyeing of textiles. In order to avoid the toxic effect due to the use of metal mordants, the present work uses enzyme for sustainable natural dyeing of wool. The current study is aimed at preparing multifunctional wool fabric using natural dye green tea (Camellia sinensis). Laccase (an enzyme) was used to polymerise the phenolic compounds of Camellia sinensis in situ on wool. The in situ colouration of wool fabric was performed at various varying dyeing conditions (temperature, time, and concentrations) using laccase. Colouration properties (colour values and strength) were examined to estimate the appearance of dyed fabrics. The evaluation of dyed fabrics for functional properties such as antibacterial, antioxidant, and UV protection was done. The efficient functional properties viz, antibacterial activity (> 75%), antioxidant property (> 90%), and excellent UV protection, were obtained. FTIR analysis of separately prepared polymeric dye and the dyed fabric was also done to confirm the laccase-assisted polymerisation. Thus, a novel approach of enzymatic functional natural dyeing of wool was explored.

Environmental friendly pollution free bio-dyeing of wool with haar singhar (Coral Jasmine) flower extract.

The world's move towards revival of eco-labelled products has created a huge urge to explore new means which are healthier for the global community. Among such means, plant-based bio-pigments for coloration of matrix are gaining worldwide fame, particularly in the textile sector. For the purpose of appraising new source of eco-friendly dyes, using microwave irradiation techniques, Coral Jasmine flowers have been explored for the bio-dyeing of wool. The colorant was extracted in acidic medium owing to nature of fabric, and both stuffs have been exposed to microwave treatment up to 5 min. Bio-coloration of MW irradiated and unirradiated wool was done using MW irradiated and unirradiated extract for observing high yield. Central composite design (CCD) as statistical method was utilized to see the significance of dyeing parameters chosen for mordanting to develop colorfast shades. Different concentrations of sustainable chemicals and bio-mordants as per weight of fabric were employed to introduce new shades with improved colorfastness properties. International standard textile methods determining shade permanency (fastness) have been employed onto selected dyed-mordanted fabrics. Good yield of colorant was observed when MW irradiated wool fabric was dyed at 75 °C for 45 min with extract of 7 pH, having 1.5g/100 mL of salt solution; the promising color yield was observed. As per gray scale ratings observed after ISO standard methods, pine nut as bio-mordant and iron salt as chemical mordant have developed colorfast shades. Conclusively, it can be recommended that methods for the isolation of colorants from new dye yielding plants, MW heating method as suitable clean technology and medicinal-based bio-mordants should be employed for getting permanent gamutes.

Sustainable dyeing of wool yarns with renewable sources.

Medical and healthcare fabrics are one of the most important fields in the textile industries worldwide. They are essential in all medical fields, especially where hygiene is required. Also, it is imperative to keep the ecosystem safe up to a great extent and also produce natural-based goods. Natural dyeing with plant sources can be claimed to be an excellent environmentally friendly procedure for providing healthcare fabrics. In this study, date seeds and Zenian were used as an eco-friendly mordant and dye, respectively, for dyeing wool yarns. Date seeds pre-treated wool yarns were dyed with the methanolic extract of Zenian by the exhaustion process at the optimum dyeing conditions of dye concentration 40 g/L, pH = 4, time = 45 min, and temperature 90 °C. The pre-treating of wool yarns with date seeds not only improved the dyeability of the samples but also enhanced the colorfastness to washing and light exposure. The results also show that the pre-treated and dyed samples have effective antibacterial activity against microorganism strains. Moreover, the data confirmed the dye bath obtained from the extract of Zenian does not represent a high environmentally polluting effect, and the elaborated dyeing process enables to reduce the biological oxygen demand (BOD) and chemical oxygen demand (COD) removal values significantly. The knowledge obtained from this work presents an appropriate promising foundation for the high-added-value application of agricultural waste products.

Green miles in dyeing technology: metal-rich pumpkin extracts in aid of natural dyes.

To reduce environmental pollution, it is essential to use green processes in dyeing and meet its requirements. Most natural dyes have a low affinity to be used in the dyeing process. To refine this limitation, the mordanting flow is necessary for many dyeing cases. Pumpkin extract as a natural, metal-rich source can be used as a bio-mordant in green dyeing of natural yarns such as wool. Two natural dyes native to Iran, Reseda luteola and madder, were employed in this study. The effectiveness of bio-mordant presence on yarns was evaluated by FTIR-ATR test from mordanted and mordanted-dyed wool samples. The study of K/S (color strength) content of dyed samples showed that increasing the dye concentration increases the amount of K/S. Fastness performance of wool dyed with pumpkin alternated from good to excellent depending on natural dye type and concentration, due to the formation of complex structures.

Wool keratin - A novel dietary protein source: Nutritional value and toxicological assessment.

Keratin derived protein (KDP) was extracted from sheep wool using high pressure microwave technology and food acids and investigated for its potential as a novel dietary protein. The proximate composition, amino acid profile, element profile, in vitro cytotoxicity and digestibility of KDP were evaluated. Nutritive effects of KDP at 50% dietary supplementation were compared with a casein-based diet in a growing rat model for 95 days. Results indicate KDP to be rich in protein (86%), amino acid cysteine (8.8 g/100 g) and element selenium (0.29 µg/g). KDP was non-cytotoxic in vitro at ≤ 2 mg/mL concentration. There were no differences in the rat's weight gain compared to the control group (P > 0.05). Overall, the inclusion of the KDP in the diet was an effective substitute for casein protein at 50% and KDP has the potential to be used in the food industry as a novel dietary protein, free of fat and carbohydrate.

Effects of the Seleno-Chitosan on Daily Gain, Wool Yield, and Blood Parameter in the Chinese Merino Sheep.

To study the effects of the seleno-chitosan on daily gain, wool yield, and blood parameters in the Chinese merino sheep in the selenium (Se)-deficient pastures. The samples of soils, forages, and tissues had been collected in the Southern Xinjiang of Northwest China. Our findings indicated that the Se contents in soils and forages from affected pastures were remarkably lower than those unaffected by the pastures (P < 0.01). The Se contents in the blood and the wool from affected Chinese merino sheep were extremely lower than those from healthy sheep. Meanwhile, the values of Hb, PCV, and PLT in affected sheep were significantly decreased (P < 0.01). The yield of wool and the growth rate in affected sheep were also remarkably reduced (P < 0.01). The affected Chinese merino sheep were orally treated by seleno-chitosan for 150 days; the Se contents in blood were remarkably increased and reached the healthy range on day 5. The blood parameters soon recovered to a healthy range on day 10. The growth rate of sheep in the treated group was remarkably higher than that in the control animals. The yield of the wool was also significantly increased in the treated group. Consequently, the Se-deprived environment caused a threat to daily gain, wool yield, and blood parameters in the Chinese merino sheep. The seleno-chitosan could not only markedly increase the Se contents blood contents, but also significantly increase the production performance (daily gain and wool yield), and release the symptoms of anemia in the Se-deprived animal.

Copaifera langsdorffii Desf. bark extract: optimisation of dyeing conditions to wool and colour fastness properties.

The ability to add value to waste materials from industrial operations has come to the attention of the wood processing industry, with reports, for example, of extracts from the bark tree conveying colour and UV protection to textile fibres. The objective of the present work was to expand our developments in this arena by using Copaifera langsdorffii Desf. bark extract as a natural dye for textile dyeing. A complete 2³-statistical experimental design and the central point was elaborated. The results showed that the optimal dyeing conditions were 98 °C, for 60 min, using undiluted bark extract. The dyed fabric was analysed by a spectrophotometer using the CIELAB system for evaluation of the colour strength. The results showed a K/S value of 5.78, and the dyed fabric had good colour fastness to rubbing and washing.

Effects of Se-Enriched Malt on the Immune and Antioxidant Function in the Se-Deprived Reclamation Merino Sheep in Southern Xinjiang.

We have found that the Reclamation merino sheep in Southern Xinjiang, China, showed emaciation, stiff limbs, instability, and sudden death, which is related to the impairment of immune function and antioxidant capacity caused by selenium (Se) deficiency. The experiments were to study the effects of Se-enriched malt on the immune and antioxidant function in Se-deprived Reclamation merino sheep in Southern Xinjiang, China. The samples of soil and forage had been collected from tested pastures, and animal tissues were also collected in tested animals. The mineral content of soil, forage, and animal tissues was measured in the collected samples. Hematological indexes and biochemical values were also examined. The findings showed that the Se contents were extremely lower in affected soil and forage than those from healthy soil and forage (P < 0.01). The Se contents in affected blood and wool were also extremely lower than those from healthy blood and wool (P < 0.01). The values in glutathione peroxidase and total antioxidant capacity in affected serum samples were also extremely lower than those from healthy serum samples, and levels of malondialdehyde, total nitric oxide synthase, and lipid peroxide were extremely higher in affected serum samples than those from healthy serum samples (P < 0.01). Meanwhile, the values of hemoglobin, packed cell volume, and platelet count from affected blood were extremely lower than those from healthy blood (P < 0.01). The levels of interleukin (IL)-1ß, IL-2, tumor necrosis factor-alpha, immunoglobulin A, and immunoglobulin G in serum were extremely decreased in the affected Reclamation merino sheep (P < 0.01). The levels of IL-6 and immunoglobulin M in serum were extremely reduced in the affected Reclamation merino sheep compared to healthy animals (P < 0.01). The animals in affected pastures were orally treated with Se-enriched malt, and the Se contents in blood were extremely increased (P < 0.01). The immune function and antioxidant indicator returned to within the healthy range. Consequently, our findings were indicated that the disorder of the Reclamation merino sheep was mainly caused by the Se deficiency in soil and forage. The Se-enriched malt could not only markedly increase the Se content in blood but also much improve the immune function and the antioxidant capacity in the Se-deprived Reclamation merino sheep.

Effects of Se-Yeast on Immune and Antioxidant in the Se-Deprived Pishan Red Sheep.

The experiments were to study the effects of Se-yeast on immune and antioxidant in Selenium(Se)-deprived Pishan red sheep in Southern Xinjiang, China. The samples of soil, forage, and animal tissues were collected, and used for measuring mineral content, physiological parameter, and biochemical values. These findings showed that the Se contents in affected soil and forage were markedly lower than those from unaffected soil and forage (P < 0.01). Se in affected blood and wool were also extremely lower than those from healthy Pishan red sheep (P < 0.01). The hemoglobin, packed cell volume, platelet count, Glutathione peroxidase, and total antioxidant capacity in the affected Pishan red sheep were markedly lower than those from healthy ones too (P < 0.01). The levels of malondialdehyde, total nitric oxide synthase, and lipid peroxide in Pishan red sheep from affected pastures were extremely higher than those from healthy ones (P < 0.01). The levels of interleukin (IL)-1ß, Interleukin-2, tumor necrosis factor-α and interleukin-6 from serum were markedly decreased in affected Pishan red sheep(P < 0.01). The Pishan red sheep in Se-deprived pasture were treated by orally with Se-yeast, the amount of Se in the blood markedly increased in treated animals. Meanwhile, the immune and antioxidant indicator was returned to the healthy values. Consequently, our findings were indicated that Se-deprived forage caused oxidative damage, and a serious threat to the immune function in animals. The Se-yeast is more effective in the Se-deficient Pishan red sheep including blood Se content, immune function and the antioxidant capacity.

Sheep Wool Humidity under Electron Irradiation Affects Wool Sorptivity towards Co(II) Ions.

The effect of humidity on sheep wool during irradiation by an accelerated electron beam was examined. Each of the samples with 10%, 53%, and 97% relative humidity (RH) absorbed a dose of 0, 109, and 257 kGy, respectively. After being freely kept in common laboratory conditions, the samples were subjected to batch Co(II) sorption experiments monitored with VIS spectrometry for different lapses from electron beam exposure. Along with the sorption, FTIR spectral analysis of the wool samples was conducted for cysteic acid and cystine monoxide, and later, the examination was completed, with pH measuring 0.05 molar KCl extract from the wool samples. Besides a relationship to the absorbed dose and lapse, the sorptivity results showed considerable dependence on wool humidity under exposure. When humidity was deficient (10% RH), the sorptivity was lower due to limited transformation of cystine monoxide to cysteic acid. The wool pre-conditioned at 53% RH, which is the humidity close to common environmental conditions, demonstrated the best Co(II) sorptivity in any case. This finding enables the elimination of pre-exposure wool conditioning in practice. Under excessive humidity of 97% RH and enough high dose of 257 kGy, radiolysis of water occurred, deteriorating the sorptivity. Each wool humidity, dose, and lapse showed a particular scenario. The time and humidity variations in the sorptivity for the non-irradiated sample were a little surprising; despite the absence of electron irradiation, relevant results indicated a strong sensitivity to pre-condition humidity and lapse from the start of the monitoring.

Microextraction of Reseda luteola-Dyed Wool and Qualitative Analysis of Its Flavones by UHPLC-UV, NMR and MS.

Detailed knowledge on natural dyes is important for agronomy and quality control as well as the fastness, stability, and analysis of dyed textiles. Weld (Reseda luteola L.), which is a source of flavone-based yellow dye, is the focus of this study. One aim was to reduce the required amount of dyed textile to ≤50 µg for a successful chromatographic analysis. The second aim was to unambiguously confirm the identity of all weld flavones. By carrying out the extraction of 50 µg dyed wool with 25 µL of solvent and analysis by reversed-phase UHPLC at 345 nm, reproducible chromatographic fingerprints could be obtained with good signal to noise ratios. Ten baseline separated peaks with relative areas ≥1% were separated in 6 min. Through repeated polyamide column chromatography and prepHPLC, the compounds corresponding with the fingerprint peaks were purified from dried weld. Each was unequivocally identified, including the position and configuration of attached sugars, by means of 1D and 2D NMR and high-resolution MS. Apigenin-4'-O-glucoside and luteolin-4'-O-glucoside were additionally identified as two trace flavones co-eluting with other flavone glucosides, the former for the first time in weld. The microextraction might be extended to other used dye plants, thus reducing the required amount of precious historical textiles.

Experience of the implementation of felt therapy classes (using of non-spun wool in the art therapy process) while working with the emotional state of patients of the FSBSI "National Medical Research Center for Rehabilitation and Balneology".

ABSTRACT: The article describes some psychological peculiarities of the work with the emotional state of patients and one of the possible ways to correct it using art therapy - felt therapy, (the use of non-spun wool in an art therapy process). In the article are described the possibilities of this material and the effects of its use. The main tasks of the work are the formation of a conscious attitude to the disease, the recognition of the "secondary benefits" of having a "symptom", the actualization, awareness and replenishment of the spectrum of internal resources, the evolution of the ability to gain the access to them, as well as the integration, acceptance of the "symptom" and the suffering part of the personality, the acceptance of the diversity of one's own "I". The art therapeutic exercise described in the article also aims to reinforce a new positively colored creative experience, the formation of positive thinking. All of these helps the patient to feel a holistic personality, more harmonious and happy.

Effects of Nano-selenium Poisoning on Immune Function in the Wumeng Semi-fine Wool Sheep.

The Wumeng semi-fine wool sheep is vital to the production system in Southwest China. To study the effects of nano-selenium (Nano-Se) poisoning in the Wumeng semi-fine wool sheep, poisoning model was established. A total of 20 animals with an average initial body weight (BW) of 35.57 ± 3.31 kg were used in this study. After 1-week-long acclimatization period, all animals were randomly divided into two groups: (1) control, with no any treatment; (2) Nano-Se group, orally administered 5 mg Nano-Se (BW/kg/day). There were 10 replications/group and one sheep/replication. The experiment lasted for 30 days. Compared with the control, hemoglobin, erythrocyte count, and packed cell volume in Nano-Se group markedly decreased (P < 0.01), and the activities of serum lactate dehydrogenase, glutamic oxaloacetic transaminase, cereal third transaminase, and alkaline phosphatase in Nano-Se group significantly increased (P < 0.01). T3 and FT3 in serum in Nano-Se group were greatly lower than those in the control (P < 0.01). Contents of T4, FT4, and TSH in serum in Nano-Se group were extremely higher than those in the control (P < 0.01). Levels of IgG, IgM, IgA, interleukin 2 (IL-2), and tumor necrosis factor-alpha (TNF-α) in Nano-Se group were much lower than those of the control (P < 0.01), but levels of interleukin 6 (IL-6) and interleukin-1ß (IL-1ß) were greatly higher than those in the control (P < 0.01). Compared with the control, serum superoxide dismutase, glutathione peroxide, total antioxidant capacity, and catalase from Nano-Se group extremely decreased (P < 0.01), and content of serum malondialdehyde in Nano-Se group markedly increased (P < 0.01). The current results indicated that the blood parameters in the Wumeng semi-fine wool sheep were affected by Nano-Se poisoning, and the immune function and antioxidant capacity were greatly reduced too.

Suplementação gestacional na produção de lã de ovelhas e cordeiros / Gestacional supplementation in wool production of ewes and lambs

O objetivo deste estudo foi avaliar o efeito da suplementação durante diferentes períodos gestacionais sobre a produção de lã de ovelhas e cordeiros da raça Ideal. Foram utilizadas 53 ovelhas da raça Ideal, com escore corporal médio (3), inseminadas pela técnica de laparoscopia, com sêmen fresco de um único reprodutor; e seus cordeiros. Os tratamentos experimentais foram: sem suplementação (n=9); suplementação do início da gestação até 50 dias (n=11); suplementação dos 51 aos 100 dias de gestação (n=11); suplementação dos 101 aos 150 dias - final da gestação (n=11); e suplementação durante toda a gestação (n=11). A suplementação foi a 1,5% do peso corporal. Amostras de lã das ovelhas e dos cordeiros foram tomadas na região do costilhar esquerdo e enviadas ao laboratório para análises objetivas de finura de lã. As suplementações no terço final e durante toda a gestação proporcionavam os melhores resultados, com aumento de produção de lã e de peso das ovelhas. Ovelhas que receberam suplementação durante toda a gestação apresentaram maior diâmetro de fibra e peso corporal. O desempenho de lã dos cordeiros não foi influenciado pela alimentação de ovelhas durante a gestação.(AU)

The objective of this study was to evaluate the effect of supplementation during different gestational periods on the wool production of sheep and lambs of the Ideal breed. Fifty-three adult Ideal sheep were used, with a mean body score inseminated by the laparoscopy technique using fresh sperm from a single breeder and his lambs. Treatment groups: No supplementation (n=9); Supplementation in the beginning of gestation up to 50 days (n=11); Supplementation from 51 to 100 days of gestation (n=11); Supplementation from 101 to 150 days - end of gestation (n=11) ;and Supplementation throughout the gestation (n=11). The supplementation was at 1.5% of body weight. After birth, lambs were kept with their mothers in cultivated pasture of black oats and ryegrass. Wool samples from sheep and lambs were taken in the left-hand region and sent to the laboratory for objective analyzes of wool fineness. The supplementation performed in the final third and throughout the gestation provides the best results, with increased sheep weight. Ewes that received supplementation throughout pregnancy had greater fiber diameter and body weight. The wool performance of lambs was not influenced by feeding sheep during gestation.(AU)

Undernutrition combined with dietary mineral oil hastens depuration of stored dioxin and polychlorinated biphenyls in ewes. 2. Tissue distribution, mass balance and body burden.

Food safety crises involving persistent organic pollutants (POPs) lead to systematic slaughter of livestock to prevent contaminants from entering the food chain. Therefore, there is a need to develop strategies to depurate livestock moderately contaminated with POPs to reduce economic and social damage. This study aimed to test undernutrition (37% of energy requirements) combined with mineral oil (10% in total dry matter intake) in nine non-lactating ewes contaminated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and polychlorinated biphenyls (PCBs) 126 and 153 as a strategy to enhance the depuration of POPs through faecal excretion. To better understand the underlying mechanisms of the depuration process, lipophilic POPs and lipid fluxes were co-monitored in various body and excretion compartments. Body compartments (adipose tissues, muscle, liver and blood) and the total empty body were analyzed for lipids and POPs concentrations and burdens at slaughter, as well as excretion compartments (faeces and wool) collected during the depuration period. Decreases in empty body total and lipid weights were 6-fold higher in underfed and supplemented ewes compared to control ewes. In addition, over the depuration period undernutrition and supplementation treatment increased faecal TCDD, PCBs 126 and 153 excretions by 1.4- to 2.1-fold but tended to decrease wool PCB 153 excretion by 1.4-fold. This induced 2- to 3-fold higher decreases in the empty body POPs burdens for underfed and supplemented ewes. Nonetheless, when expressed relative to the calculated initial empty body burdens, burdens at slaughter decreased only slightly from 97%, 103% and 98% for control ewes to 92%, 97% and 94% for underfed and supplemented ones, for TCDD, PCBs 126 and 153, respectively. Fine descriptions at once of POPs kinetic (companion paper 1) and mass balance (companion paper 2), and of body lipid dynamics were very useful in improving our understanding of the fate of POPs in the ruminants.

Traditional Yellow Dyes Used in the 21st Century in Central Iran: The Knowledge of Master Dyers Revealed by HPLC-DAD and UHPLC-HRMS/MS.

This work provides new knowledge on natural yellows used in Iran. Seven biological sources were selected based on interviews with dye masters in Isfahan workshops (Iran). Delphinium semibarbatum, Eremostachys laevigata, Prangos ferulacea, Morus alba, Pistacia vera, Punica granatum, and Vitis vinifera are currently used in these workshops. Aiming to study the dye composition of wool samples dyed with the extracts of the selected biological sources and the changes induced by the dyeing procedures in the original chemical composition of the plant extract, raw materials and dyed wool (by us and in the workshops) were analyzed by HPLC-DAD and UHPLC-HRMS/MS. The main yellows for E. laevigata are luteolin-O-glycosides. In the other plant sources, the main chromophores are based on 3-O-glycosides of kaempferol, quercetin, and isorhamnetin. In pistachio hulls, myricitin derivatives were detected and we propose their use as markers. Generally, the solutions extracted from the wool displayed a higher amount of more polar compounds, but also a higher amount of aglycones. Importantly, the chromatographic profiles of the samples we prepared compared well with 17th c. yellows in Persian carpets, and therefore can be considered highly characterized references for the study of Persian yellows.

Studies of Selenium Deficiency in the Wumeng Semi-Fine Wool Sheep.

Wumeng semi-fine wool sheep are affected by a disease, characterized by emaciation, stiffness and trembling of the limbs, weakness and inability to stand, and sudden death. The objective of the study was to determine possible relationships between the disease and mineral deficiencies. Samples of wool, blood, and liver were collected from affected and healthy sheep. Samples of soil and forage were collected from affected and unaffected areas. The samples were used for hematological and biochemical analyses and mineral nutrient measurements. Results showed that selenium concentrations in forage and soil samples from affected areas were significantly lower than those from unaffected areas (P < 0.01). Meanwhile, selenium concentrations of wool, blood, and liver from the affected sheep were also significantly lower than those from the healthy sheep (P < 0.01). The mean concentration of hemoglobin (Hb), packed cell volume (PCV), and mean corpuscular hemoglobin (MCH) from the affected sheep were significantly lower than those from the healthy sheep (P < 0.01). Serum glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activity in the affected sheep were significantly lower than those in the healthy sheep (P < 0.01). Serum creatine phosphokinase (CPK), lactate dehydrogenase (LDH), glutamate pyruvate transaminase (GPT), glutamic oxaloacetic transaminase (GOT), alkaline phosphatase (ALP), and malondialdehyde (MDA) values in the affected sheep were significantly higher than those in the healthy sheep (P < 0.01). Serum concentrations of free triiodothyronine (FT3) and triiodothyronine (TT3) in the affected sheep were significantly lower than those in the healthy sheep; serum concentrations of free tetraiodothyronine (FT4) and tetraiodothyronine (TT4) in the affected sheep were significantly higher than those in the healthy sheep (P < 0.01). But the administration of selenium and vitamin E by hypodermic injection prevented and cured the disease. The injection contains 0.1% and 5% of sodium selenite and vitamin E, respectively. A single dose is 6, 6, and 2 mL for mature ewe, mature ram, and lamb, respectively, repeated only once 15 days later. This study demonstrated that the disorder of Wumeng semi-fine wool sheep was mainly caused by the selenium deficiency in soil and forage.