RESUMEN
OBJECTIVES: To examine the effects of aging and/or diabetes mellitus on oxidative stress and the protective effect of vitamin E in the bladder. It was proposed that the balance between oxidant and antioxidant species is important regarding the aging process and prevention of diabetic complications. METHODS: Young and aged rats were randomly allotted into six experimental groups: aged control, aged diabetic, aged diabetic and vitamin E-treated, young control, young diabetic, young diabetic and vitamin E-treated. Diabetes was induced by streptozotocin. Vitamin E was administered to the treated groups. Malondialdehyde and reduced glutathione levels were measured in all rat bladders, and histological changes were examined by electron microscopy. RESULTS: We found increased malondialdehyde and decreased glutathione levels in the young and aged diabetic groups compared with the nondiabetic control groups. Elevated malondialdehyde and reduced glutathione levels were observed in the aged compared with the young control groups. There were no significant differences in the malondialdehyde and glutathione levels between young and aged diabetic vitamin E-treated groups compared with the related control groups. Degeneration was greatest in the aged diabetic group. The protective effects of vitamin E were seen in young and aged diabetic groups, especially in the young diabetic group. CONCLUSIONS: Our results suggest that vitamin E supplementation prevents free radical damage in bladders of young and aged diabetic rats.
Asunto(s)
Antioxidantes/uso terapéutico , Diabetes Mellitus Experimental/metabolismo , Estrés Oxidativo/efectos de los fármacos , Vejiga Urinaria/metabolismo , Vitamina E/uso terapéutico , Factores de Edad , Animales , Masculino , Ratas , Ratas WistarRESUMEN
OBJECTIVE: To investigate the effects of aluminium sulphate on the microscopic morphology of the liver and on vitamin E amelioration of aluminium-induced liver damage. METHODS: Rats were injected intraperitoneally with aluminium sulphate alone or aluminium sulphate together with vitamin E, with saline injected rats used as the control group. The study took place in Pamukkale University Faculty of Medicine in 2005. RESULTS: The rats exposed to aluminium showed morphological changes in addition to previously reported biochemical changes in the liver. The anti-oxidant vitamin E significantly diminished the liver damage seen due to aluminium. CONCLUSION: There is an apparent protective effect of vitamin E on parenteral aluminium exposure.
Asunto(s)
Compuestos de Alumbre/toxicidad , Hepatopatías/prevención & control , Hígado/patología , Vitamina E/farmacología , Compuestos de Alumbre/farmacología , Animales , Biopsia con Aguja , Enfermedad Hepática Inducida por Sustancias y Drogas , Suplementos Dietéticos , Modelos Animales de Enfermedad , Interacciones Farmacológicas , Inmunohistoquímica , Inyecciones Intraperitoneales , Hígado/efectos de los fármacos , Hepatopatías/patología , Masculino , Estrés Oxidativo/efectos de los fármacos , Distribución Aleatoria , Ratas , Ratas Wistar , Valores de Referencia , Sensibilidad y EspecificidadRESUMEN
Thirty adult male mice were divided into three groups. The animals in group I were used as controls and drank only water during the entire period of experimentation. Group II animals drank water containing 1.5 g/100 mL zinc as ZnSO4, and group III animals received 2.5 g/100 mL zinc. After 3 wk supplementation with high doses of zinc, the animals were killed and the livers were removed and examined by electron microscopic techniques. After the supplementation period, the animals in groups II and II showed various degrees of degenerative changes in the hepatocytes, such as increased size and the presence of spaces and an abundance of lipid globules in the cytoplasm. The mitochondria showed a crystalline appearance, a diluted matrix, and dense aggregations. Some smooth endoplasmic reticulum tubules showed dilation and were filled with a dense substance. None of these changes were present in the group I control animals.
Asunto(s)
Hígado/efectos de los fármacos , Hígado/ultraestructura , Zinc/envenenamiento , Animales , Enfermedad Hepática Inducida por Sustancias y Drogas , Sobredosis de Droga , Hepatocitos/efectos de los fármacos , Hepatocitos/patología , Hepatocitos/ultraestructura , Hígado/citología , Hígado/patología , Hepatopatías/patología , Masculino , Ratones , Ratones Endogámicos BALB C , Microscopía Electrónica , Orgánulos/efectos de los fármacos , Orgánulos/patología , Orgánulos/ultraestructuraRESUMEN
The purpose of this study was to investigate the effects of excessive zinc intake on the testes and on sperm count and motility in mice. Thirty Balb c mice were divided randomly into 3 groups of 10 animals in each. Group I acted as controls; group II was supplied with drinking water containing 1.5 g/100 mL Zn, and group III was supplied with drinking water containing 2.5 g/100 mL Zn. The animals were sacrificed after 3 wk supplementation and the epididymis and testis were quickly excised. A negative correlation between Zn dose and sperm count and motility was found. The sperm count in group III was significantly lower than in groups II and I (p<0.05). The sperm motility in group III was significantly lower than in the controls (p<0.05). Degenerative changes, including spermatic arrest, degeneration of seminiferous tubules, and fibrosis in interstitial tissue, were observed in group III animals. These results show that high doses of zinc significantly alter sperm motility.