RESUMEN
Asymmetric mesoporous silica nanoparticles with a head-tail structure are potent immunoadjuvants for delivering a peptide antigen, generating a higher antibody immune response in mice compared to their symmetric counterparts.
Asunto(s)
Adyuvantes Inmunológicos/química , Anticuerpos/inmunología , Nanopartículas/química , Péptidos/administración & dosificación , Péptidos/inmunología , Dióxido de Silicio/química , Adyuvantes Inmunológicos/administración & dosificación , Adyuvantes Inmunológicos/farmacocinética , Animales , Reacciones Antígeno-Anticuerpo , Supervivencia Celular , Células HEK293 , Humanos , Ratones , Nanopartículas/administración & dosificación , Nanopartículas/metabolismo , Tamaño de la Partícula , Péptidos/química , Porosidad , Células RAW 264.7 , Dióxido de Silicio/inmunología , Dióxido de Silicio/farmacocinética , Propiedades de SuperficieRESUMEN
Mesoporous silica nanoparticles are reported as adjuvants in nanovaccines in generating robust antigen-specific immunity. However, the effect of surface chemistry in initiating and modulating the immune response remains largely unexplored. In this study, mesoporous silica nanorods (MSNRs) are modified with NH2 and C18 groups to investigate the influence of surface functional groups (OH, NH2 , and C18 ) on their adjuvant efficacy. It is found that compared to OH and NH2 groups, the hydrophobic C18 modification significantly enhances antigen uptake by antigen presenting cells and endosomal-lysosomal escape in vitro, dendritic cells, and macrophages maturation ex vivo, and elicits secretion of interferon-γ level and antibody response in immunized mice. Moreover, bare MSNR and MSNRNH2 exhibit T-helper 2 biased immune response, while MSNRC18 shows a T-helper 1 biased immune response. These findings suggest that the surface chemistry of nanostructured adjuvants has profound impact on the immune response, which provides useful guidance for the design of effective nanomaterial based vaccines.