Chemoprofiling and antimicrobial activity of medicinal herbs used in the treatment of inflammatory bowel disease.

Inflammatory bowel disease (IBD) is a term utilized to illustrate two different chronic disorders of the gastro-intestinal tract i.e., Crohn's disease and ulcerative colitis. The symptoms of IBD are mainly characterized by inflammation, including abdominal pain, chronic diarrhoea, weight loss, shortening of the colon and rectal bleeding. The objective of this study was to evaluate the antimicrobial activity and Gas Chromatography-Mass Spectrometry (GC-MS) analysis of herbs used in the treatment of IBD in Saudi Arabia. Ethanolic extracts of five different herbs from Saudi Arabia namely Pimpinella anisum (Anise), Foeniculum vulgare (Fennel), Matricaria chamomilla (Chamomile), Linum usitatissimum (Linseed), and Punica granatum (Pomegranate) were prepared by Soxhlet extraction. The systemic chemical composition of the extracts was identified by GC-MS with their relative concentrations. The ethanolic extract of P. anisum, F. vulgare, M. chamomilla, L. usitatissimum, and P. granatum showed the presence of 35, 42, 34, 37, and 47 chemical components in these extracts, respectively. The five extracts and an equal mixture of them were examined for their antimicrobial activity by broth dilution method against different organisms. These included Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis and Pseudomonas aeruginosa) bacteria and one yeast (Candida albicans). P. anisum, F. vulgare, M. chamomilla, L. usitatissimum, P. granatum and the mixture of all five extracts had good activity against E. coli (MIC=3.125, 0.050, 6.25, 0.050 and 0.100 mg/ml, respectively). P. granatum also had a MIC of 3.125 mg/ml against S. aureus. In conclusion. the plants' extracts and an equal mixture of them showed a narrow spectrum of antimicrobial activity against S. aureus, K. pneumoniae, P. mirabilis, P. aeruginosa and C. albicans.

Pasteurella multocida toxin activates human monocyte-derived and murine bone marrow-derived dendritic cells in vitro but suppresses antibody production in vivo.

Pasteurella multocida toxin (PMT) is a potent mitogen for fibroblasts and osteoblastic cells. PMT activates phospholipase C-beta through G(q)alpha, and the activation of this pathway is responsible for its mitogenic activity. Here, we investigated the effects of PMT on human monocyte-derived dendritic cells (MDDC) in vitro and show a novel activity for PMT. In this regard, PMT activates MDDC to mature in a dose-dependent manner through the activation of phospholipase C and subsequent mobilization of calcium. This activation was accompanied by enhanced stimulation of naive alloreactive T cells and dominant inhibition of interleukin-12 production in the presence of saturating concentrations of lipopolysaccharide. Surprisingly, although PMT mimics the activating effects of cholera toxin on human MDDC and mouse bone marrow-derived dendritic cells, we found that PMT is not a mucosal adjuvant and that it suppresses the adjuvant effects of cholera toxin in mice. Together, these results indicate discordant effects for PMT in vitro compared to those in vivo.