RESUMEN
Histochemical studies and SEM observations on the morphological changes in the head of the spermatozoa in general, and the acrosome in particular, in A. indica treated rats are reported. In the treated rats change in the shape and size of the sperm head, with a dorso-ventral constriction of the middle region of the sperm head i.e., between the anterior and posterior regions was observed. It was rather difficult to differentiate the outer acrosomal and outer plasma membranes. A decrease in the perforatorium or sub-acrosomal material, post nuclear cap and the nuclear material near the basal plate at the base of the sperm head were also observed. The results suggest that the effects are probably due to androgen deficiency and a general disturbance in carbohydrates or polysaccharides located in the sperm head, caused by the antiandrogenic property of the leaves of A. indica.
Asunto(s)
Plantas Tóxicas , Espermatozoides/efectos de los fármacos , Árboles , Acrosoma/efectos de los fármacos , Acrosoma/patología , Antagonistas de Andrógenos/toxicidad , Animales , Masculino , Microscopía Electrónica de Rastreo , Extractos Vegetales/toxicidad , Hojas de la Planta , Ratas , Ratas Wistar , Espermatozoides/patologíaRESUMEN
Histological and biochemical changes in the testis of rats treated with the leaf powder of A. indica are reported. The pattern of recovery is also studied at 8, 16 and 24 day after withdrawal of the treatment. In the treated rats, a general reduction in the diameters of seminiferous tubule, nuclei of the germinal elements and a mass atrophy of the spermatogenic elements has been observed. The Leydig cells are found to be atrophic. Biochemically, a decrease in the protein content and the activity of acid phosphatase and an increase in the total free sugar, glycogen, cholesterol contents and the activities of alkaline phosphatase and lactate dehydrogenase have been observed. A gradual recovery is observed in both the histological and biochemical parameters after 8.16 and 24 day of cessation of the treatment. The result suggest a possible reversible antiandrogenic property of the leaves of A. indica in male albino rats.
Asunto(s)
Plantas Tóxicas , Testículo/efectos de los fármacos , Antagonistas de Andrógenos/aislamiento & purificación , Antagonistas de Andrógenos/toxicidad , Animales , Masculino , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/toxicidad , Plantas Medicinales , Ratas , Ratas Wistar , Testículo/metabolismo , Testículo/patología , Factores de TiempoRESUMEN
The histological and biochemical changes in the caput and cauda epididymis of albino rat treated with 20, 40 and 60 mg dry powder of the leaves of A. indica per day for 24 days are reported. In the treated rats, the height of the epithelium and the diameter of the nucleus in both the regions were reduced. The lumen of the caput was packed with lymphocytes. Biochemically, a decrease in the protein content and acid phosphatase activity and an increase in the alkaline phosphatase and lactate dehydrogenase activities were observed in both the regions. The effect was dose dependent. Further, serum testosterone concentration in the higher dose treated animals decreased significantly. The results suggest a possible antiandrogenic property of the leaves of A. indica.