Application of imidazolium based ionic liquids grafted on microcrystalline cellulose as demulsifiers for water in crude oil (W/O) emulsions.

Separation of water and oil from water-in-oil (W/O) emulsion before transportation and refining is very important and critical. Ionic liquids (ILs) and their derivatives have recently attracted much attention as efficient chemical agents for breaking emulsions. So, here, a series of microcrystalline cellulose (MCC) grafted with imidazolium-based ionic liquids (IM-ILs) were synthesized, named as MCC@IM-ILs, and evaluated as eco-friendly surface-active agents for the separation of water from the crude oil. Structure of the synthesized compounds was confirmed by different characterization techniques. Dehydration efficiency percent (DE%) of the synthesized demulsifiers was measured and compared with each other. Synthesized MCC@IM-ILs showed an acceptable DE% to demulsify three kinds of W/O emulsions with different water content after 5 min. Concentration, alkyl chain length, and counter-anion of the synthesized MCC@IM-ILs play a key role in separating water from crude oil. Demulsifier with C10 alkyl chain length showed better DE% than the corresponding demulsifier with C6 alkyl chain length in the W/O (30:70 v/v) emulsion. Also, demulsifier with Br counter anion showed lower DE% than the corresponding BF4 ion-exchanged compound with higher hydrophilicity. Synthesized demulsifiers immobilized on ILs have significant advantages compared to unsupported ILs due to the use of green and economical cellulosic substrate.

Biochemical and insecticidal effects of plant essential oils on insecticide resistant and susceptible populations of Musca domestica L. point to a potential cross-resistance risk.

Essential oils (EOs) can provide important alternatives to chemical insecticides in the control of pests. In this study, 12 EOs of native plant species from Iran were evaluated for their adulticidal activity against the house fly. In addition, we examined the insecticidal activity of Zataria multiflora and Rosmarinus officinalis EOs on adult female house flies from pyrethroid and organophosphate resistant and susceptible populations, using both fumigant and topical bioassays. The involvement of detoxification enzymes in susceptibility was investigated with synergism experiments in vivo, while the inhibitory effects of R. officinalis and Zataria multiflora EOs on the activities of cytochrome P450-dependent monooxygenases (P450s), carboxylesterases (CarEs) and glutathione S-transferases (GSTs) were determined by enzymatic inhibition assays in vitro. The EOs of Z. multiflora, Mentha pulegium, R. officinalis and Thymus vulgaris were the most effective against adults in contact topical assays, while oils extracted from Eucalyptus cinerea, Z. multiflora, Citrus sinensis, R. officinalis, Pinus eldarica and Lavandula angustifolia where the most effective in fumigant assays. Rosmarinus officinalis and Z. multiflora EOs were selected for further investigation and showed higher toxicity against a susceptible population, compared to two insecticide-resistant populations. Correlation analysis suggested cross-resistance between these EOs and pyrethroids in the resistant populations. The toxicity of both EOs on the resistant populations was synergized by three detoxification enzyme inhibitors. Further, in vitro inhibition studies showed that R. officinalis and Z. multiflora EOs more effectively inhibited the activities of the detoxification enzymes from flies of the susceptible population compared to those of the pyrethroid resistant populations. Synergistic and enzymatic assays further revealed that increased activities of P450s, GSTs, and CarEs are possibly involved in the cross-resistance between EOs and pyrethroids. Investigating the molecular mechanisms of P450s, GSTs, and CarEs in the resistance to EOs should be subject to further studies.

Antioxidants and glycine can improve the developmental competence of vitrified/warmed ovine immature oocytes.

Despite the numerous potential applications of oocyte cryopreservation, the poor success rate has limited its practical applications. In livestock, particularly in ovine, the oocytes have low developmental competence following vitrification/warming process. Considering the occurrence of osmotic and oxidative stresses during the vitrification/warming process, the application of antioxidants and osmolytes may improve the developmental competence of vitrified/warmed oocytes. In the present study, we aimed to evaluate the effects of the addition of ascorbic acid (AA) and N-acetyl cysteine (NAC) as antioxidants and glycine as an organic osmolyte either to the vitrification/warming solutions (VWS) or to the IVM medium on the developmental competence of vitrified/warmed ovine germinal vesicle stage oocytes. The survival rate in the vitrified groups was significantly lower than fresh ones. In vitrified/warmed oocytes, there was no significant difference in survival rate between supplemented and non-supplemented groups. The addition of AA and/or NAC to the VWS or IVM medium and adding glycine to the IVM medium reduced the proportion of apoptotic oocytes and fragmented embryos, which was reflected as an increase in the proportions of metaphase II stage oocytes and blastocyst production. The best result was achieved by supplementing the IVM medium with NAC. In our study condition, antioxidants and glycine could improve the developmental competence of vitrified/warmed ovine immature oocytes, especially when added during IVM.