Protein Profiling of Aedes aegypti Treated with Streptomyces sp. KSF103 Ethyl Acetate Extract Reveals Potential Insecticidal Targets and Metabolic Pathways.

The insecticidal activity of Streptomyces sp. KSF103 ethyl acetate (EA) extract against mosquitoes is known; however, the underlying mechanism behind this activity remains elusive. In this study, liquid chromatography with tandem mass spectrometry (LC-MS/MS) was employed to investigate changes in the protein profile of Aedes aegypti larvae and adults treated with lethal concentrations of 50 (LC50) EA extract. By comparing the treated and untreated mosquitoes, this study aimed to identify proteins or pathways that exhibit alterations, potentially serving as targets for future insecticide development. Treatment with a lethal concentration of EA extract upregulated 15 proteins in larvae, while in adults, 16 proteins were upregulated, and two proteins were downregulated. These proteins were associated with metabolism, protein regulation/degradation, energy production, cellular organization and structure, enzyme activity, and catalysis, as well as calcium ion transport and homeostasis. Notably, ATP synthase, fructose-bisphosphate aldolase (FBA), and ATP citrate synthase were significantly expressed in both groups. Gene ontology analysis indicated a focus on energy metabolic processes. Molecular docking revealed a strong interaction between dodemorph, selagine (compounds from the EA extract), and FBA, suggesting FBA as a potential protein target for insecticide development. Further studies such as Western blot and transcriptomic analyses are warranted to validate the findings.

Insecticidal activities of Streptomyces sp. KSF103 ethyl acetate extract against medically important mosquitoes and non-target organisms.

A potentially novel actinobacterium isolated from forest soil, Streptomyces sp. KSF103 was evaluated for its insecticidal effect against several mosquito species namely Aedes aegypti, Aedes albopictus, Anopheles cracens and Culex quinquefasciatus. Mosquito larvae and adults were exposed to various concentrations of the ethyl acetate (EA) extract for 24 h. Considerable mortality was evident after the EA extract treatment for all four important vector mosquitoes. Larvicidal activity of the EA extract resulted in LC50 at 0.045 mg/mL and LC90 at 0.080 mg/mL for Ae. aegypti; LC50 at 0.060 mg/mL and LC90 at 0.247 mg/mL for Ae. albopictus; LC50 at 2.141 mg/mL and LC90 at 6.345 mg/mL for An. cracens; and LC50 at 0.272 mg/mL and LC90 at 0.980 mg/mL for Cx. quinquefasciatus. In adulticidal tests, the EA extract was the most toxic to Ae. albopictus adults (LD50 = 2.445 mg/mL; LD90 = 20.004 mg/mL), followed by An. cracens (LD50 = 5.121 mg/mL; LD90 = 147.854 mg/mL) and then Ae. aegypti (LD50 = 28.873 mg/mL; LD90 = 274.823 mg/mL). Additionally, the EA extract exhibited ovicidal activity against Ae. aegypti (LC50 = 0.715 mg/mL; LC90 = 6.956 mg/mL), Ae. albopictus (LC50 = 0.715 mg/mL; LC90 = 6.956 mg/mL), and An. cracens (LC50 = 0.715 mg/mL; LC90 = 6.956 mg/mL), evaluated up to 168 h post-treatment. It displayed no toxicity on the freshwater microalga Chlorella sp. Beijerinck UMACC 313, marine microalga Chlorella sp. Beijerinck UMACC 258 and the ant Odontoponera denticulata. In conclusion, the EA extract showed promising larvicidal, adulticidal and ovicidal activity against Ae. aegypti, Ae. albopictus, An. cracens, and Cx. quinquefasciatus (larvae only). The results suggest that the EA extract of Streptomyces sp. KSF103 has the potential to be used as an environmental-friendly approach in mosquito control. The current study would serve as an initial step toward complementing microbe-based bioinsecticides for synthetic insecticides against medically important mosquitoes.

Streptomyces derivatives as an insecticide: Current perspectives, challenges and future research needs for mosquito control.

The pervasiveness of arboviruses in wreaking havoc on public health has lingered on international health agendas. A scarcity of mosquito-borne disease vaccines and therapies demands prompt attention, as billions of people worldwide are at risk of infections. It is widely known that vector control continues, and in some diseases, remains the only resort in suppressing disease transmissions we presently possess at its disposal. But the use of commercial insecticides is being crippled by the widespread insecticide resistance, which greatly menaces their efficacies, toxicological repercussions such as environmental pollution and human health risk. Rather, an environmentally benign technique of employing Streptomyces isolates from settings such as terrestrial soils, marine sediments, and mangrove soils for Culicidae management has recently received a lot of positive attention. Streptomyces' capacities to produce a wide range of bioactive secondary metabolites that contribute to pharmaceutical, agricultural and veterinarian, Streptomyces-derived bioactive compounds are increasingly being considered for use in vector control. Herein, we compiled all of the available datasets on the effectiveness of Streptomyces-derived compounds against major mosquito vectors of medical importance. Aedes, Anopheles, and Culex are used to assess the toxicity of crude extracts or fractions. This paper reviewed the promising ovicidal, larvicidal, and pupacidal effects of different Streptomyces strains. Notably, no research into the adulticidal effect of Streptomyces-derived compounds has yet been done. Aside from the genetic makeup, the production of secondary metabolites from Streptomyces depends on the growing conditions. And that, to optimise the maximum yield of highly potent bioactive compounds being extracted, solvents' choice is of paramount importance. Thus, both cultivation parameters and the choice of organic solvents for secondary metabolites extraction will be discussed. Furthermore, biases derived from different studies have implied the need for standardizing experimental procedures. While entomological data should be collected consistently across all studies to expedite evidence-based policymaking of bioinsecticides, the quality of data from vector control interventions - particularly the experimental design, execution, analysis, and presentation of results of vector control studies - will be thoroughly reviewed. Lastly, to promote consistency and reliability, these knowledge gaps are identified, along with a discussion of current perspectives on vector control, global bioinsecticide trends, challenges on commercializing bioinsecticides and future research needs.