RESUMEN
Sugar cane extract (SCE) has been shown to have an immunostimulating effect in chickens. This study evaluated the effect of SCE on Salmonella Abortusequi lipopolysaccharide (LPS)-induced lethal shock in d-galactosamine (GalN)-sensitized mice. Mice were administered intraperitoneally SCE (500 mg/kg) or phosphate buffered saline before or after injection of LPS and GalN. All the mice injected with LPS and GalN (control group) died of histopathologically congestive and hemorrhagic hepatic insufficiency within 24 h, showing significantly increased activities of plasma aspartate aminotransferase (AST; 380 IU/mL) and alanine aminotransferase (ALT; 130 IU/mL). Pretreatment of mice with SCE at 3 h before challenge with LPS and GalN (SCE treated group) resulted in significantly improved survival rates (92.3%) and a decrease in liver injury. These surviving mice in the SCE treated group showed no changes in the mean levels of plasma AST (60 IU/mL) and ALT (18 IU/mL). However, the level of tumor necrosis factor-alpha in the SCE treated group was not significantly different when compared with that in the control group challenged with LPS and GalN. These results suggest that SCE has protective effects on LPS-induced mortality in this mouse model.
Asunto(s)
Antibacterianos/farmacología , Fitoterapia , Extractos Vegetales/farmacología , Saccharum , Choque Séptico/tratamiento farmacológico , Animales , Antibacterianos/administración & dosificación , Antibacterianos/uso terapéutico , Modelos Animales de Enfermedad , Eritrocitos/efectos de los fármacos , Femenino , Galactosamina/farmacología , Hemorragia/inducido químicamente , Inyecciones Intraperitoneales , Lipopolisacáridos , Hígado/enzimología , Hígado/patología , Ratones , Ratones Endogámicos BALB C , Extractos Vegetales/administración & dosificación , Extractos Vegetales/uso terapéutico , Salmonella , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
To evaluate the radioprotective effect of sugar cane extract (SCE), SCE was orally administered into the crop of 3-week-old chickens for 3 consecutive days before or after x-ray radiation at a dose of 500 mg/kg/day. The survival rate of SCE administered chickens before x-ray radiation at a dose of 920 rad increased to 68.8% when compared with that of the irradiated control (50%). Histopathological examination revealed the intestine of SCE administered chickens to have mild to moderate pathological changes, when compared with that of the irradiated control animals.
Asunto(s)
Fitoterapia , Extractos Vegetales/farmacología , Traumatismos Experimentales por Radiación/prevención & control , Protectores contra Radiación/farmacología , Saccharum , Administración Oral , Animales , Pollos , Yeyuno/patología , Extractos Vegetales/administración & dosificación , Extractos Vegetales/uso terapéutico , Dosis de Radiación , Protectores contra Radiación/administración & dosificación , Protectores contra Radiación/uso terapéutico , Rayos XRESUMEN
The present study was undertaken to evaluate the effect of sugar cane (Saccharum officinarum L.) extract (SCE) on the immune system of X-ray immunosuppressed chickens. SCE (500 mg/kg/day) was administrated into the crop of 3-week-old chickens for three consecutive days before or after irradiation. The results indicated that administration of SCE before or after whole body X-ray irradiation enhanced both primary and secondary immune responses in chickens immunized with sheep red blood cells and Brucella abortus (BA) as well as cell-mediated immunity measured by delayed type hypersensitivity to human gamma-globulin.