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1.
Int J Mol Sci ; 24(11)2023 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-37298579

RESUMEN

Multiple abiotic stress is known as a type of environmental unfavourable condition maximizing the yield and growth gap of crops compared with the optimal condition in both natural and cultivated environments. Rice is the world's most important staple food, and its production is limited the most by environmental unfavourable conditions. In this study, we investigated the pre-treatment of abscisic acid (ABA) on the tolerance of the IAC1131 rice genotype to multiple abiotic stress after a 4-day exposure to combined drought, salt and extreme temperature treatments. A total of 3285 proteins were identified and quantified across the four treatment groups, consisting of control and stressed plants with and without pre-treatment with ABA, with 1633 of those proteins found to be differentially abundant between groups. Compared with the control condition, pre-treatment with the ABA hormone significantly mitigated the leaf damage against combined abiotic stress at the proteome level. Furthermore, the application of exogenous ABA did not affect the proteome profile of the control plants remarkably, while the results were different in stress-exposed plants by a greater number of proteins changed in abundance, especially those which were increased. Taken together, these results suggest that exogenous ABA has a potential priming effect for enhancing the rice seedlings' tolerance against combined abiotic stress, mainly by affecting stress-responsive mechanisms dependent on ABA signalling pathways in plants.


Asunto(s)
Ácido Abscísico , Oryza , Ácido Abscísico/farmacología , Ácido Abscísico/metabolismo , Oryza/genética , Proteoma/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Estrés Fisiológico/genética , Regulación de la Expresión Génica de las Plantas , Plantas Modificadas Genéticamente/genética , Sequías
2.
Proteomics ; 22(19-20): e2100247, 2022 10.
Artículo en Inglés | MEDLINE | ID: mdl-35866514

RESUMEN

Fingolimod (FTY720) is an oral drug approved by the Food and Drug Administration (FDA) for management of multiple sclerosis (MS) symptoms, which has also shown beneficial effects against Alzheimer's (AD) and Parkinson's (PD) diseases pathologies. Although an extensive effort has been made to identify mechanisms underpinning its therapeutic effects, much remains unknown. Here, we investigated Fingolimod induced proteome changes in the cerebellum (CB) and frontal cortex (FC) regions of the brain which are known to be severely affected in MS, using a tandem mass tag (TMT) isobaric labeling-based quantitative mass-spectrometric approach to investigate the mechanism of action of Fingolimod. This study identified 6749 and 6319 proteins in CB and FC, respectively, and returned 2609 and 3086 differentially expressed proteins in mouse CB and FC, respectively, between Fingolimod treated and control groups. Subsequent bioinformatics analyses indicated a metabolic reprogramming in both brain regions of the Fingolimod treated group, where oxidative phosphorylation was upregulated while glycolysis and pentose phosphate pathway were downregulated. In addition, modulation of neuroinflammation in the Fingolimod treated group was indicated by upregulation of retrograde endocannabinoid signaling and autophagy pathways, and downregulation of neuroinflammation related pathways including neutrophil degranulation and the IL-12 mediated signaling pathway. Our findings suggest that Fingolimod may exert its protective effects on the brain by inducing metabolic reprogramming and neuroinflammation pathway modulation.


Asunto(s)
Clorhidrato de Fingolimod , Esclerosis Múltiple , Animales , Ratones , Clorhidrato de Fingolimod/farmacología , Clorhidrato de Fingolimod/metabolismo , Clorhidrato de Fingolimod/uso terapéutico , Proteoma/metabolismo , Endocannabinoides/metabolismo , Encéfalo/metabolismo , Esclerosis Múltiple/metabolismo , Metabolismo Energético , Autofagia , Interleucina-12/metabolismo
3.
Biomolecules ; 11(10)2021 09 27.
Artículo en Inglés | MEDLINE | ID: mdl-34680044

RESUMEN

Cannabis (Cannabis sativa), popularly known as marijuana, is the most commonly used psychoactive substance and is considered illicit in most countries worldwide. However, a growing body of research has provided evidence of the therapeutic properties of chemical components of cannabis known as cannabinoids against several diseases including Alzheimer's disease (AD), multiple sclerosis (MS), Parkinson's disease, schizophrenia and glaucoma; these have prompted changes in medicinal cannabis legislation. The relaxation of legal restrictions and increased socio-cultural acceptance has led to its increase in both medicinal and recreational usage. Several biochemically active components of cannabis have a range of effects on the biological system. There is an urgent need for more research to better understand the molecular and biochemical effects of cannabis at a cellular level, to understand fully its implications as a pharmaceutical drug. Proteomics technology is an efficient tool to rigorously elucidate the mechanistic effects of cannabis on the human body in a cell and tissue-specific manner, drawing conclusions associated with its toxicity as well as therapeutic benefits, safety and efficacy profiles. This review provides a comprehensive overview of both in vitro and in vivo proteomic studies involving the cellular and molecular effects of cannabis and cannabis-derived compounds.


Asunto(s)
Cannabinoides/uso terapéutico , Cannabis/genética , Proteoma/genética , Proteómica , Enfermedad de Alzheimer/tratamiento farmacológico , Analgésicos/uso terapéutico , Agonistas de Receptores de Cannabinoides/uso terapéutico , Cannabinoides/genética , Glaucoma/tratamiento farmacológico , Humanos , Esclerosis Múltiple/tratamiento farmacológico , Enfermedad de Parkinson/tratamiento farmacológico , Proteoma/efectos de los fármacos , Esquizofrenia/tratamiento farmacológico
4.
Plant Cell Environ ; 44(7): 2150-2166, 2021 07.
Artículo en Inglés | MEDLINE | ID: mdl-33047317

RESUMEN

The development of gametes in plants is acutely susceptible to heatwaves as brief as a few days, adversely affecting pollen maturation and reproductive success. Pollen in cotton (Gossypium hirsutum) was differentially affected when tetrad and binucleate stages were exposed to heat, revealing new insights into the interaction between heat and pollen development. Squares were tagged and exposed to 36/25°C (day/night, moderate heat) or 40/30°C (day/night, extreme heat) for 5 days. Mature pollen grains and leaves were collected for physiological and proteomic responses. While photosynthetic competence was not compromised even at 40°C, leaf tissues became leakier. In contrast, pollen grains were markedly smaller after the tetrad stage was exposed to 40°C and boll production was reduced by 65%. Sugar levels in pollen grains were elevated after exposure to heat, eliminating carbohydrate deficits as a likely cause of poor reproductive capacity. Proteomic analysis of pure pollen samples revealed a particularly high abundance of 70-kDa heat shock (Hsp70s) and cytoskeletal proteins. While short-term bursts of heat had a minor impact on leaves, male gametophyte development was profoundly damaged. Cotton acclimates to maxima of 36°C at both the vegetative and reproductive stages but 5-days exposure to 40°C significantly impairs reproductive development.


Asunto(s)
Gossypium/crecimiento & desarrollo , Gossypium/metabolismo , Respuesta al Choque Térmico/fisiología , Proteínas de Plantas/metabolismo , Polen/crecimiento & desarrollo , Electrólitos/metabolismo , Proteínas de Choque Térmico/metabolismo , Fotosíntesis , Hojas de la Planta/metabolismo , Polen/metabolismo , Semillas/metabolismo , Almidón/metabolismo , Sacarosa/metabolismo , Azúcares/metabolismo , Termotolerancia/fisiología
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