RESUMEN
OBJECTIVE: To develop a new method for simultaneous determination of oleanolic acid and llrolic acid in Chinese medicinal herbs at the same time. METHOD: HPLC was carried out on a Shim-pack CLC-ODS column using MeOH-H2O-HOAc-TEA (83:17:0.04:0.02). RESULT AND CONCLUSION: The average recovery of oleanolic acid and llrolic acid was 103.3 +/- 2.07% and 102.7% +/- 0.65% respectively.
Asunto(s)
Ácido Oleanólico/análisis , Plantas Medicinales/química , Triterpenos/análisis , Cromatografía Líquida de Alta Presión/métodos , Hedyotis/química , Ligustrum/química , Lycopus/química , Ácido UrsólicoRESUMEN
Two new diterpenes, named triptoditerpenic acid B(2) and hypodiolide A(3), respectively, were isolated from Tripterygium hypoglaucum (Lévl.) Hutch. Their structures were elucidated on the basis of spectra and X-ray analysis.
Asunto(s)
Diterpenos/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Fenantrenos , Diterpenos/química , Conformación Molecular , Estructura MolecularRESUMEN
A new diterpene, named Triptonoditerpenic acid. C21H28O4, mp 189-190 degrees C, has been isolated from Tripterygium hypoglaucum (Levl) Hutch. Its structure was elucidated by UV, IR, MS, 1HNMR, 13CNMR and 2D-NMR spectroscopic analyses.
Asunto(s)
Diterpenos/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Diterpenos/químicaRESUMEN
A novel algorithm of target factor analysis has been developed for detection and correction of unknown absorptive background in multicomponent analysis. The algorithm is based on the property that the estimated spectra can gradually approach the true ones by iterative refinements. Paracetamol and antipyrine contained in compound injection of paracetamol were determined by this method without any preliminary chemical separation. The average recoveries were both 100.0% and the coefficients of variation were 1.1% and 1.0% respectively. The results clearly indicate that the proposed method may also provide a new approach to the analysis of traditional Chinese medicine containing some unknown absorptive components.
Asunto(s)
Acetaminofén/análisis , Antipirina/análisis , Algoritmos , Combinación de Medicamentos , Espectrofotometría Ultravioleta/métodos , Comprimidos/análisisRESUMEN
Pyrolysis-high-resolution gas chromatography-pattern recognition (Py-HRGC-PaRe) was used to develop a potential technique for identifying the Chinese traditional medicine Mai Dong. About 1 mg of crude drug powder was pyrolysed in a furnace pyrolyser and the products were directly carried into a gas chromatograph with an FSOT capillary column (30 m x 0.265 mm I.D.) coated with DB-1701 (df 0.25 micron). The Py-HRGC data were analysed by non-linear mapping PaRe. The results showed that Mai Dong samples could be classified into two categories: Ophiopogon japonicus (L.f.) Ker-Gawl (included in the Chinese Pharmacopoeia) and Liriope spicata.
Asunto(s)
Medicamentos Herbarios Chinos/química , Cromatografía de Gases , Reconocimiento de Normas Patrones AutomatizadasRESUMEN
Thirty-eight samples of Moutan (Paeonia suffruticosa Andr.) Cortex obtained from three different regions (Southwest, East and Middle China) and two samples of unknown region were subjected to analysis with pyrolysis-high resolution gas chromatography (Py-HRGC). Each sample was thus characterized by the peak area of 41 peaks in each Py-HRGC profile. Discriminant analysis (DA), PRIMA and SIMCA pattern recognition were used to recognize the 40 x 41 data matrix. These data analysis gave satisfactory results (DA, 100% correct; PRIMA, 100% correct, 92.2% unique; SIMCA, 92.2% correct, 79% unique). The correct classification of Moutan Cortex for the unknown territory was obtained by three pattern recognition methods. The results showed that Py-HRGC/pattern recognition technique might be a potential tool for the identification of Chinese traditional medicine.
Asunto(s)
Medicamentos Herbarios Chinos/clasificación , Cromatografía de Gases/métodos , Reconocimiento de Normas Patrones AutomatizadasAsunto(s)
Medicamentos Herbarios Chinos , Ácido Glicirretínico/análogos & derivados , Ácido Glicirretínico/sangre , Zinc/administración & dosificación , Animales , Cromatografía Líquida de Alta Presión , Ácido Glicirretínico/administración & dosificación , Ácido Glicirrínico , Conejos , Espectrofotometría UltravioletaRESUMEN
Berberine (1) and palmatine (2) in cortex phellodendron and Chinese patent medicines were determined by HPLC. The optimal composition of mobile phase CH3COOEt-HCOOH-EtOH (15:3:2) for HPLC separation of berberine and palmatine was successfully determined by using window diagram technique. Detection wavelength was 346nm. Flow rate: 1.5 ml/min. Calibration graphs for 1 and 2 were rectilinear for 0.06-0.32 micrograms and 0.12-0.32 micrograms respectively. The average recoveries of the two corresponding components were found to be over 96% and their coefficients of variation were below 5%.
Asunto(s)
Alcaloides de Berberina/análisis , Berberina/análisis , Medicamentos Herbarios Chinos/análisis , Cromatografía Líquida de Alta PresiónRESUMEN
A normal phase liquid chromatographic method was developed for the determination of berberine-type alkaloids in rhizoma Coptidis and traditional Chinese medicine--Angong Niuhuang Wan. Experimental evidences indicate that a normal phase system of a silica column (3.9 x 250 mm) as stationary phase with an eluent of ethyl acetate-formic acid-ethanol (15:3:2) as mobile phase can separate excellently (R5 greater than 1.5) four berberine-type alkaloids in the samples tested. According to the results obtained with this method the percent content of berberine hydrochloride in some Angong Niuhuang Wan from different factories and different batches was 0.331-0.456% and the average recovery was 97.23%; coefficient of variation was 1.2%.