RESUMEN
Shrubs have developed various mechanisms for soil phosphorus utilization. Shrub encroachment caused by climate warming alters organic phosphorus mineralization capability by promoting available phosphorus absorption and mediating root exudates. However, few studies have explored how warming regulates the effects of dominant shrubs on soil organic phosphorus mineralization capability. We provide insights into warming, dominant shrub removal, and their interactive effects on the soil organic phosphorus mineralization potential in the Qinghai-Tibetan Plateau. Real-time polymerase chain reaction was used to quantify the soil microbial phosphatase genes (phoC and phoD), which can characterize the soil organic phosphate mineralization potential. We found that warming had no significant effect on the soil organic phosphate-mineralized components (total phosphate, organic phosphate, and available phosphate), genes (phoC and phoD), or enzymes (acid and alkaline phosphatases). Shrub removal negatively influenced the organic phosphate-mineralized components and genes. It significantly decreased soil organic phosphate mineralization gene copy numbers only under warming conditions. Warming increased fungal richness and buffered the effects of shrub removal on bacterial richness and gene copy numbers. However, the change in the microbial community was not the main factor affecting organic phosphate mineralization. We found only phoC copy number had significant correlation to AP. Structural equation modelling revealed that shrub removal and the interaction between warming and shrub removal had a negative direct effect on phoC copy numbers. We concluded that warming increases the negative effect of shrub removal on phosphorus mineralization potential, providing a theoretical basis for shrub encroachment on soil phosphate mineralization under warming conditions.
Asunto(s)
Bacterias , Fósforo , Fósforo/análisis , Suelo/química , Fosfatos/análisis , Organofosfatos , Microbiología del SueloRESUMEN
TIFY is a plant-specific gene family with four subfamilies: ZML, TIFY, PPD, and JAZ. Recently, this family was found to have regulatory functions in hormone stimulation, environmental response, and development. However, little is known about the roles of the TIFY family in Tartary buckwheat (Fagopyrum tataricum), a significant crop for both food and medicine. In this study, 18 TIFY family genes (FtTIFYs) in Tartary buckwheat were identified. The characteristics, motif compositions, and evolutionary relationships of the TIFY proteins, as well as the gene structures, cis-acting elements, and synteny of the TIFY genes, are discussed in detail. Moreover, we found that most FtTIFYs responded to various abiotic stresses (cold, heat, salt, or drought) and hormone treatments (ABA, MeJA, or SA). Through yeast two-hybrid assays, we revealed that two FtTIFYs, FtTIFY1 and FtJAZ7, interacted with FtABI5, a homolog protein of AtABI5 involved in ABA-mediated germination and stress responses, implying crosstalk between ABA and JA signaling in Tartary buckwheat. Furthermore, the overexpression of FtJAZ10 and FtJAZ12 enhanced the heat stress tolerance of tobacco. Consequently, our study suggests that the FtTIFY family plays important roles in responses to abiotic stress and provides two candidate genes (FtJAZ10 and FtJAZ12) for the cultivation of stress-resistant crops.
Asunto(s)
Fagopyrum , Fagopyrum/metabolismo , Filogenia , Proteínas de Plantas/metabolismo , Estrés Fisiológico/genética , Hormonas/metabolismo , Regulación de la Expresión Génica de las PlantasRESUMEN
Histone deacetylases (HDACs), widely found in various types of eukaryotic cells, play crucial roles in biological process, including the biotic and abiotic stress responses in plants. However, no research on the HDACs of Fagopyrum tataricum has been reported. Here, 14 putative FtHDAC genes were identified and annotated in Fagopyrum tataricum. Their gene structure, motif composition, cis-acting elements, phylogenetic relationships, protein structure, alternative splicing events, subcellular localization and gene expression pattern were investigated. The gene structure showed FtHDACs were classified into three subfamilies. The promoter analysis revealed the presence of various cis-acting elements responsible for hormone, abiotic stress and developmental regulation for the specific induction of FtHDACs. Two duplication events were identified in FtHDA6-1, FtHDA6-2, and FtHDA19. The expression patterns of FtHDACs showed their correlation with the flavonoid synthesis pathway genes. In addition, alternative splicing, mRNA enrichment profiles and transgenic analysis showed the potential role of FtHDACs in cold responses. Our study characterized FtHDACs, providing a candidate gene family for agricultural breeding and crop improvement.
Asunto(s)
Fagopyrum , Fagopyrum/genética , Fagopyrum/metabolismo , Regulación de la Expresión Génica de las Plantas , Histona Desacetilasas/metabolismo , Filogenia , Fitomejoramiento , Proteínas de Plantas/metabolismo , TemperaturaRESUMEN
Understanding the response of soil properties and bacterial communities in rhizosphere soil to aridity and dune types is fundamental to desertification control. This study investigated soil properties and bacterial communities of both rhizosphere and bulk soils of Caragana microphylla from four sites with different aridity indices, and one site with three different types of dunes. All sites were located in the desert regions of northern China. The results indicated that compared with the bulk soil, the soil nutrient content of rhizosphere, especially the content of total phosphorus, was generally significantly improved in different desertification environments. The bacterial richness and diversity were also higher than those of bulk soil, especially in arid regions and fixed dunes. Firmicutes, Actinobacteria, Proteobacteria, and Acidobacteria were the most dominant phyla in all samples. The regression analyses showed that at different sites, soil total organic C, total N, Na+, and total P played key roles in determining the bacterial community structure while total organic carbon, electronic conductivity, pH and total phosphorus were the dominant factors at the different dunes. The results further revealed that the dominant phyla strongly affected by environmental factors at different sites were Acidobacteria, Gemmatimonadetes, and Actinobacteria among which, Acidobacteria and Gemmatimonadetes were negatively correlated with Na+ content. At different types of dunes, Actinobacteria, Planctomycetes, and Gemmatimonadetes were particularly affected by environmental factors. The increased abundance of Actinobacteria in the rhizosphere soil was mainly caused by the decreased soil pH.
Asunto(s)
Bacterias/aislamiento & purificación , Caragana/microbiología , Microbiología del Suelo , Acidobacteria/genética , Acidobacteria/aislamiento & purificación , Actinobacteria/genética , Actinobacteria/aislamiento & purificación , Bacterias/genética , Biodiversidad , Caragana/crecimiento & desarrollo , Conservación de los Recursos Naturales , Clima Desértico , Conductividad Eléctrica , Concentración de Iones de Hidrógeno , Fósforo/química , Raíces de Plantas/microbiología , Proteobacteria/genética , Proteobacteria/aislamiento & purificación , ARN Ribosómico 16S/química , ARN Ribosómico 16S/genética , ARN Ribosómico 16S/metabolismo , Rizosfera , Suelo/químicaRESUMEN
A strain of Nocardia isolated from crude oil-contaminated soils in the Qinghai-Tibetan Plateau degrades nearly all components of crude oil. This strain was identified as Nocardia soli Y48, and its growth conditions were determined. Complete genome sequencing showed that N. soli Y48 has a 7.3â¯Mb genome and many genes responsible for hydrocarbon degradation, biosurfactant synthesis, emulsification and other hydrocarbon degradation-related metabolisms. Analysis of the clusters of orthologous groups (COGs) and genomic islands (GIs) revealed that Y48 has undergone significant gene transfer events to adapt to changing environmental conditions (crude oil contamination). The structural features of the genome might provide a competitive edge for the survival of N. soli Y48 in oil-polluted environments and reflect the adaptation of coexisting bacteria to distinct nutritional niches.
Asunto(s)
Genes Bacterianos , Nocardia/genética , Petróleo/metabolismo , Biodegradación Ambiental , Islas Genómicas , Hidrocarburos/metabolismo , Nocardia/metabolismo , Microbiología del Suelo , Tensoactivos/metabolismoRESUMEN
A Gram-stain-positive, aerobic, non-motile and mycolic-acid-containing strain, designated Y48T, was isolated from soil contaminated by crude oil located in the northern margin of the Qaidam Basin. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y48T belongs to the genus Nocardia and is closely related to N. cummidelens DSM 44490T (99.0â% similarity), N. soli DSM 44488T (99.0â%), N. lasii 3C-HV12T (98.9â%), N. salmonicida NBRC 13393T (98.6â%), N. ignorata NBRC 108230T (98.6â%) and N. coubleae NBRC 108252T (98.6â%). The average nucleotide identity and DNA-DNA hybridization values between strain Y48T and the reference strains were 75.9-84.5 and 27.5-29.0â%, respectively, values that were below the thresholds for species delineation. Chemotaxonomic analysis indicated that the major fatty acids of strain Y48T were C16â:â0, summed feature 3 (C16â:â1ω6c/C16â:â1ω7c), C18â:â1ω9c and C18â:â0 10-methyl (TBSA). The respiratory quinone was MK-8(H4, ω-cycl). The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, two glycolipids and three unidentified lipids. The cell-wall hydrolysates contained meso-diaminopimelic acid, with ribose, arabinose, glucose and galactose as whole-cell sugars. A combination of 16S rRNA gene sequence analysis, and phenotypic and chemotaxonomic characterizations demonstrated that strain Y48T represents a novel species of the genus Nocardia, for which the name Nocardia mangyaensis sp. nov. is proposed. The type strain is Y48T (=JCM 32795T=CGMCC 4.7494T).
Asunto(s)
Nocardia/clasificación , Contaminación por Petróleo , Filogenia , Microbiología del Suelo , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácido Diaminopimélico/química , Ácidos Grasos/química , Nocardia/aislamiento & purificación , Hibridación de Ácido Nucleico , Petróleo , Fosfolípidos/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Contaminantes del Suelo , Tibet , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMEN
Desert plants are thought to rely more heavily on nutrient resorption due to the infertile soil. However, little is known regarding the phylogenetic effects on this traits, specifically for halophytes. Here we determined contents of nitrogen (N), phosphorus (P), potassium (K), sodium (Na), calcium (Ca) and magnesium (Mg) in 36 desert plants in a hyper-arid environment. The patterns of resorption or accumulation of the six elements were compared among plant groups with diverse leaf Na regulation strategies: i.e., euhalophytes (Eu), secretohalophytes (Se), pseudohalophytes (Ps) and glycophytes (Gl). Overall, N, P, K presented strict resorption across all groups, but no more efficient than global estimations. Ca and Mg tended to be resorbed less or accumulated during leaf senescence. Significant phylogenetic signal of both leaf Na content and plant group implies the pivotal role of Na regulation in the adaptation of plants to desert environment. Resorption proficiency, rather than resorption efficiency, is more phylogenetically conservative and more relevant to leaf functional traits.
Asunto(s)
Nutrientes/metabolismo , Hojas de la Planta/metabolismo , Plantas Tolerantes a la Sal/metabolismo , Sodio/metabolismo , Ecosistema , Fósforo/metabolismo , Filogenia , Hojas de la Planta/química , Potasio/metabolismo , Análisis de Componente Principal , Plantas Tolerantes a la Sal/química , Plantas Tolerantes a la Sal/clasificaciónRESUMEN
Pseudarthrobacter sulfonivorans strain Ar51, a psychotrophic bacterium isolated from the Tibet permafrost of China, can degrade crude oil and multi benzene compounds efficiently in low temperature. Here we report the complete genome sequence of this bacterium. The complete genome sequence of Pseudarthrobacter sulfonivorans strain Ar51, consisting of a cycle chromosome with a size of 5.04Mbp and a cycle plasmid with a size of 12.39kbp. The availability of this genome sequence allows us to investigate the genetic basis of crude oil degradation and adaptation to growth in a nutrient-poor permafrost environment.
Asunto(s)
Derivados del Benceno/metabolismo , Micrococcaceae/genética , Micrococcaceae/metabolismo , Petróleo/metabolismo , Biotecnología , Hielos Perennes/microbiologíaRESUMEN
The villin/gelsolin/fragmin superfamily is a major group of Ca2+-dependent actin-binding proteins (ABPs) involved in various cellular processes. Members of this superfamily typically possess three or six tandem gelsolin-like (G) domains, and each domain plays a distinct role in actin filament dynamics. Although the activities of most G domains have been characterized, the biochemical function of the G3 domain remains poorly understood. In this study, we carefully compared the detailed biochemical activities of ABP29 (a new member of this family that contains the G1-G2 domains of lily ABP135) and ABP135G1-G3 (which contains the G1-G3 domains of lily ABP135). In the presence of high Ca2+ levels in vitro (200 and 10 µM), ABP135G1-G3 exhibited greater actin severing and/or depolymerization and nucleating activities than ABP29, and these proteins had similar actin capping activities. However, in the presence of low levels of Ca2+ (41 nM), ABP135G1-G3 had a weaker capping activity than ABP29. In addition, ABP29 inhibited F-actin depolymerization, as shown by dilution-mediated depolymerization assay, differing from the typical superfamily proteins. In contrast, ABP135G1-G3 accelerated F-actin depolymerization. All of these results demonstrate that the G3 domain plays specific roles in regulating the activities of the lily villin/gelsolin/fragmin superfamily proteins.
Asunto(s)
Actinas/química , Gelsolina/química , Proteínas de Microfilamentos/química , Citoesqueleto de Actina/química , Animales , Sitios de Unión , Calcio/química , ADN Complementario/metabolismo , Dalteparina/química , Humanos , Lilium/química , Microscopía Fluorescente , Familia de Multigenes , Músculo Esquelético/metabolismo , Dominios y Motivos de Interacción de Proteínas , Estructura Terciaria de Proteína , Conejos , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes/químicaRESUMEN
Salinization is an important and increasingly prevalent issue which has broad and profound effects on plant survival and distribution pattern. To understand the patterns and potential drivers of leaf traits in saline environments, we determined the soil properties, leaf morphological traits (specific leaf area, SLA, and leaf dry matter content, LDMC), leaf chemical traits (leaf carbon, C, nitrogen, N, and phosphorus, P, stoichiometry) based on 142 observations collected from 23 sites in an arid saline environment, which is a vulnerable ecosystem in northwest China. We also explored the relationships among leaf traits, the responses of leaf traits, and plant functional groups (herb, woody, and succulent woody) to various saline environments. The arid desert halophytes were characterized by lower leaf C and SLA levels, higher N, but stable P and N:P. The leaf morphological traits were correlated significantly with the C, N, and P contents across all observations, but they differed within each functional group. Succulent woody plants had the lowest leaf C and highest leaf N levels among the three functional groups. The growth of halophytes might be more limited by N rather than P in the study area. GLM analysis demonstrated that the soil available nutrients and plant functional groups, but not salinity, were potential drivers of leaf C:N:P stoichiometry in halophytes, whereas species differences accounted for the largest contributions to leaf morphological variations. Our study provides baseline information to facilitate the management and restoration of arid saline desert ecosystem.
Asunto(s)
Carbono/análisis , Nitrógeno/análisis , Fósforo/análisis , Hojas de la Planta/química , Poaceae/química , Salinidad , Plantas Tolerantes a la Sal/fisiología , Ecosistema , Ambiente , Poaceae/crecimiento & desarrollo , Suelo/químicaRESUMEN
We measured the influences of soil fertility and plant community composition on Glomeromycota, and tested the prediction of the functional equilibrium hypothesis that increased availability of soil resources will reduce the abundance of arbuscular mycorrhizal (AM) fungi. Communities of plants and AM fungi were measured in mixed roots and in Elymus nutans roots across an experimental fertilization gradient in an alpine meadow on the Tibetan Plateau. As predicted, fertilization reduced the abundance of Glomeromycota as well as the species richness of plants and AM fungi. The response of the glomeromycotan community was strongly linked to the plant community shift towards dominance by Elymus nutans. A reduction in the extraradical hyphae of AM fungi was associated with both the changes in soil factors and shifts in the plant community composition that were caused by fertilization. Our findings highlight the importance of soil fertility in regulating both plant and glomeromycotan communities, and emphasize that high fertilizer inputs can reduce the biodiversity of plants and AM fungi, and influence the sustainability of ecosystems.
Asunto(s)
Ecosistema , Fertilizantes , Glomeromycota/efectos de los fármacos , Glomeromycota/fisiología , Nitrógeno/farmacología , Fósforo/farmacología , Biodiversidad , Biomasa , Elymus/efectos de los fármacos , Elymus/microbiología , Hifa/efectos de los fármacos , Hifa/fisiología , Modelos Lineales , Micorrizas/efectos de los fármacos , Micorrizas/fisiología , Suelo/química , Especificidad de la Especie , Esporas Fúngicas/efectos de los fármacos , Esporas Fúngicas/fisiología , TibetRESUMEN
Plastic film mulching (PFM) is a widely used agricultural practice in the temperate semi-arid Loess Plateau of China. However, how beneficial soil microbes, arbuscular mycorrhizal (AM) fungi in particular, respond to the PFM practice is not known. Here, a field experiment was performed to study the effects of a 3-month short-term PFM practice on AM fungi in plots planted with spring wheat (Triticum aestivum L. cv. Dingxi-2) in the Loess Plateau. AM colonization, spore density, wheat spike weight, and grain phosphorus (P) content were significantly increased in the PFM treatments, and these changes were mainly attributable to changes in soil properties such as available P and soil moisture. Alkaline phosphatase activity was significantly higher in PFM soils, but levels of AM fungal-related glomalin were similar between treatments. A total of nine AM fungal phylotypes were detected in root samples based on AM fungal SSU rDNA analyses, with six and five phylotypes in PFM and no-PFM plots, respectively. Although AM fungal phylotype richness was not statistically different between treatments, the community compositions were different, with four and three specific phylotypes in the PFM and no-PFM plots, respectively. A significant and rapid change in AM fungal, wheat, and soil variables following PFM suggested that the functioning of the AM symbiosis had been changed in the wheat field under PFM. Future studies are needed to investigate whether PFM applied over a longer term has a similar effect on the AM fungal community and their functioning in an agricultural ecosystem.
Asunto(s)
Agricultura/métodos , Micorrizas/fisiología , Triticum/microbiología , Secuencia de Bases , China , ADN de Hongos/química , ADN de Hongos/genética , Grano Comestible/microbiología , Grano Comestible/fisiología , Proteínas Fúngicas/análisis , Hongos/genética , Hongos/aislamiento & purificación , Hongos/fisiología , Glicoproteínas/análisis , Datos de Secuencia Molecular , Micorrizas/genética , Micorrizas/aislamiento & purificación , Fósforo/análisis , Filogenia , Raíces de Plantas/microbiología , Raíces de Plantas/fisiología , Plásticos , ARN de Hongos/genética , ARN Ribosómico/genética , Lluvia , Análisis de Secuencia de ADN , Suelo/química , Microbiología del Suelo , Esporas Fúngicas/fisiología , Triticum/fisiologíaRESUMEN
With the combination of root staining and PCR-DGGE, the seasonal variation of arbuscular mycorrhizal fungi (AMF) in Caragana korshinskii roots was investigated; and by the methods of principal component analysis and canonical correspondence analysis, the relationships between this variation and soil factors were analyzed. It was found that the total infection rate of AMF and the infection rates of arbuscules and vesicles in C. korshinskii roots as well as the spore density of AMF in soil varied significantly among seasons. From spring to autumn, the total infection rate of AMF had a decreasing trend, whereas the other three indicators were in adverse. A total of nine AMF phylotypes were detected in spring, summer, and autumn, but the community composition and diversity indices of AMF had definite differences among the seasons. The infection rate of vesicles decreased with increasing soil total N, P, and organic C, while the spore density of AMF increased with decreasing soil moisture and available P. The seasonal variation of AMF community composition in C. korshinskii roots was mainly affected by soil total P, available P, and organic C.
Asunto(s)
Caragana/microbiología , Micorrizas/crecimiento & desarrollo , Fósforo/análisis , Raíces de Plantas/microbiología , Suelo/análisis , Carbono/análisis , Nitrógeno/análisis , Análisis de Componente Principal , Estaciones del AñoRESUMEN
The ability of iron to accept and donate electrons makes it important for plant growth, but it can also damage plants when they are under environmental stress. Ferritin, a protein encoded by the gene Fer, catalyzes the oxidation of Fe(2+) and subsequent storage of Fe(3+) within the mineral core. Ferritin may reduce the adverse effects of iron on Chorispora bungeana Fisch. & C.A. May during the course of cold stress. C. bungeana is a rare alpine subnival plant species that is highly resistant to a freezing environment. We have isolated and characterized the ferritin cDNA (CbFer) from C. bungeana. It is 975 bp in length with an open reading frame of 260 amino acids, corresponding to a protein of predicted molecular mass of 29.17 kDa and an isoelectric point of 5.44. Amino acid analysis of the polypeptides indicated that CbFer codes for a ferritin subunit plus a chloroplast-targeting transit peptide. Reverse transcription polymerase chain reaction analysis confirmed that CbFer was a tissue-specific gene since the expression could only be detected in leaves. The gene expression patterns were investigated in relation to cold stress (4 degrees C and -4 degrees C) and to various exogenous signals, including excessive iron, hydrogen peroxide (H(2)O(2)), and nitrogen monoxidum (NO). The amount of CbFer mRNA increased in response to low temperatures and gene expression at -4 degrees C was both more distinct and quicker than that at 4 degrees C. Two exogenous signals, excessive iron and H(2)O(2), upregulated the expression of the CbFer gene, but NO had no effect. The CbFer gene may play an important role in response to cold stress, while the expression of the gene during stress may be influenced by major and minor factors such as iron and H(2)O(2), respectively.
Asunto(s)
Brassicaceae/genética , Frío , Ferritinas/genética , Perfilación de la Expresión Génica , Proteínas de Plantas/genética , Adaptación Fisiológica , Secuencia de Aminoácidos , Secuencia de Bases , Brassicaceae/metabolismo , Clonación Molecular , ADN Complementario/química , ADN Complementario/genética , ADN Complementario/aislamiento & purificación , Ferritinas/clasificación , Ferrozina/farmacología , Congelación , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Peróxido de Hidrógeno/farmacología , Hierro/metabolismo , Hierro/farmacología , Modelos Biológicos , Datos de Secuencia Molecular , Nitroprusiato/farmacología , Filogenia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Factores de TiempoRESUMEN
The Liqian people in north China are well known because of the controversial hypothesis of an ancient Roman mercenary origin. To test this hypothesis, 227 male individuals representing four Chinese populations were analyzed at 12 short tandem repeat (STR) loci and 12 single nucleotide polymorphisms (SNP). At the haplogroup levels, 77% Liqian Y chromosomes were restricted to East Asia. Principal component (PC) and multidimensional scaling (MDS) analysis suggests that the Liqians are closely related to Chinese populations, especially Han Chinese populations, whereas they greatly deviate from Central Asian and Western Eurasian populations. Further phylogenetic and admixture analysis confirmed that the Han Chinese contributed greatly to the Liqian gene pool. The Liqian and the Yugur people, regarded as kindred populations with common origins, present an underlying genetic difference in a median-joining network. Overall, a Roman mercenary origin could not be accepted as true according to paternal genetic variation, and the current Liqian population is more likely to be a subgroup of the Chinese majority Han.
Asunto(s)
Cromosomas Humanos Y/genética , Emigración e Inmigración/historia , Personal Militar/historia , China , Haplotipos/genética , Historia Antigua , Humanos , Masculino , Repeticiones de Microsatélite/genética , Filogenia , Análisis de Componente Principal/historia , Ciudad de RomaRESUMEN
OBJECTIVE: To study the effects and the mechanisms of extract from a leguminous plant (Ammopiptanthus mongolicus cheng f.) (JA1) in northwest China on inducing apoptosis and inhibiting proliferation of HepG2 hepatocarcinoma cell in vitro. METHODS: The HepG2 cell line was used as target cells. The effect of JA1 on HepG2 cell growth was detected by microculture tetrazolium assay (MTT), flow cytometry assay, DNA agarose gel electrophoresis and transmission electronic microscopy. The expressive effect of the wt-p53 in HepG2 cells was analyzed with p53 protein test-reagent. RESULTS: JAI not only had significant anti-proliferative effects depending upon time and dosage, but also induced apoptosis of HepG2 cells. Apoptotic typical morphological changes were observed in JA1-treated HepG2 cells under transmission electronic microscope, "Sub-G1" phase peak occurred in flow cytometry and DNA "ladder" was found in DNA agarose gel electrophoresis. The expression of the wt-p53 increased in vitro, and JA1-treated HepG2 and the positive cell percentage of the wt-p53 protein also increased. CONCLUSIONS: JA1 could obviously induce apoptosis and inhibit proliferation of HepG2 cells in vitro, and these effects are closely related with the increase of wt-p53 expression. JA1 can be used as a good source of medicinal plant for the treatment of hepatocarcinoma.
Asunto(s)
Apoptosis/efectos de los fármacos , Fabaceae/química , Extractos Vegetales/farmacología , Proteína p53 Supresora de Tumor/efectos de los fármacos , Carcinoma Hepatocelular , Ciclo Celular/efectos de los fármacos , Línea Celular Tumoral , Humanos , Proteína p53 Supresora de Tumor/metabolismoRESUMEN
In order to determine the response of pollen to UV-B irradiation and the cumulative effects of UV-B exposure time on pollen germination and tube growth of plants, 19 species were investigated in vitro by exposing their pollen grains to two levels of enhanced ultravioet-B(UV-BBE) (280-320 nm, 350 and 500 mW/m2 biologically effective UV-B radiation), simulating 8% and 21% stratospheric ozone depletion in Lanzhou, China (36.04 degrees N, 1550 m), and to no UV-B (control group). Compared with the control, enhanced UV-B radiation significantly inhibited pollen germination and tube growth in most species. Higher UV-B flux rate caused greater inhibitory effect than lower UV-B radiation level. Several test plants exhibited insensitivity, even stimulation of pollen germination and tube growth to UV-B. The reduction in pollen germination rate and tube growth increased with exposure time, which indicated a cumulative effect of UV-B radiation. It is concluded that changes in pollen susceptibility to UV-B would have a serious ecological consequence under natural conditions.