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1.
Mar Biotechnol (NY) ; 26(2): 276-287, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38441733

RESUMEN

The present study aims to characterize and to evaluate the biological effects of a skin dressing manufactured with the organic part of the Chondrilla caribensis marine sponge (called spongin-like collagen (SC)) associated or not to photobiomodulation (PBM) on the skin wound healing of rats. Skin dressings were manufactured with SC and it was characterized using scanning electron microscopy (SEM) and a tensile assay. In order to evaluate its biological effects, an experimental model of cutaneous wounds was surgically performed. Eighteen rats were randomly distributed into three experimental groups: control group (CG): animals with skin wounds but without any treatment; marine collagen dressing group (DG): animals with skin wounds treated with marine collagen dressing; and the marine collagen dressing + PBM group (DPG): animals with skin wounds treated with marine collagen dressing and PBM. Histopathological, histomorphometric, and immunohistochemical evaluations (qualitative and semiquantitative) of COX2, TGFß, FGF, and VEGF were done. SEM demonstrates that the marine collagen dressing presented pores and interconnected fibers and adequate mechanical strength. Furthermore, in the microscopic analysis, an incomplete reepithelialization and the presence of granulation tissue with inflammatory infiltrate were observed in all experimental groups. In addition, foreign body was identified in the DG and DPG. COX2, TGFß, FGF, and VEGF immunostaining was observed predominantly in the wound area of all experimental groups, with a statistically significant difference for FGF immunostaining score of DPG in relation to CG. The marine collagen dressing presented adequate physical characteristics and its association with PBM presented favorable biological effects to the skin repair process.


Asunto(s)
Vendajes , Colágeno , Poríferos , Piel , Cicatrización de Heridas , Animales , Cicatrización de Heridas/efectos de la radiación , Ratas , Colágeno/metabolismo , Piel/efectos de la radiación , Terapia por Luz de Baja Intensidad , Masculino , Factor A de Crecimiento Endotelial Vascular/metabolismo , Ciclooxigenasa 2/metabolismo , Modelos Animales de Enfermedad , Ratas Wistar , Factor de Crecimiento Transformador beta/metabolismo , Resistencia a la Tracción , Factores de Crecimiento de Fibroblastos/metabolismo , Microscopía Electrónica de Rastreo
2.
ABCS health sci ; 48: e023232, 14 fev. 2023. ilus, graf
Artículo en Inglés | LILACS | ID: biblio-1537358

RESUMEN

INTRODUCTION: Skeletal muscle atrophy leads to a reduction in muscle strength, functionality, and the quality of life of individuals. OBJECTIVE: To explore the effects of two different wavelengths (red and infrared) of laser PBMT on muscle atrophy and its active ingredients on skeletal muscle atrophy using an in vivo model of muscle atrophy. METHODS: Thirty-two Wistar rats were randomly divided into four experimental groups: control (CG) animals were not immobilized and did not receive any type of treatment; immobilized animals with no treatment (ImC); immobilized animals submitted to red laser with wavelength of 660 nm (ImR) and near-infrared laser with wavelength of 808 nm (ImIR) treatments. The treatments were applied daily, at 2 points in the right gastrocnemius muscle (cranial and caudal), through the punctual contact technique, for 9 sessions, with the first application immediately after removing the cast. RESULTS: The histological results demonstrated that in both treated groups (red and infrared wavelengths) a reduction of the inflammatory infiltrate and less connective tissue thickening when compared to the ImC. However, only infrared light was observed regenerating muscle fibers and an increase in the number of oxidative fibers (type I). CONCLUSION: These results suggest that red and infrared wavelength laser PBMT were able to promote changes in the morphology of the gastrocnemius muscle submitted to atrophy in an experimental immobilization model, reducing the inflammatory infiltrate and the formation of intramuscular connective tissue. However, infrared laser PBMT promoted more evident positive effects by increasing regenerating muscle fibers and the number of oxidative fibers.


Asunto(s)
Animales , Ratas , Fototerapia , Atrofia Muscular/radioterapia , Atrofia Muscular/terapia , Terapia por Luz de Baja Intensidad , Inmovilización
3.
Lasers Med Sci ; 38(1): 36, 2023 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-36626000

RESUMEN

Epidermal growth factor (EGF) and light-emitting diode (LED) are currently deployed as promissory treatments for skin repair; however, the mechanisms of their association are not yet evidenced. Thus, the present study aimed to evaluate the effects of combined treatment with EGF and red LED on the wound healing processes in rats. Adult Wistar rats were randomized in control group (CG) wounds without treatment; wounds submitted to EGF treatment (EGF); wounds submitted to LED treatment (LED); wounds submitted to EGF associated with LED treatments (EGF/LED). Treatments were performed immediately after the surgical procedure and each 24 h, totaling 8 sessions. Moreover, LED was applied before EGF treatment at a single point in the center of the wound. Morphological characteristics and the immunoexpression of COX-2, VEGF, and TGF-ß were measured. The results demonstrated that EGF/LED group presented a higher wound healing index. Additionally, all experimental groups presented similar findings in the histological evaluation, the degree of inflammation, and the area of dermis-like tissue. However, for EGF-treated animals (with or without LED), neoepithelial length was higher. Furthermore, all the treated groups decreased COX-2 and increased VEGF immunoexpression, and only EGF/LED group enhanced the TGF-ß protein expression when compared to the untreated group. This research shows that EGF and LED modulate inflammatory process and increase the vascularity. In addition, treatment of EGF associated with LED promoted a more evident positive effect for increasing TGF-ß expression and may be promising resources in the clinical treatment of cutaneous wounds.


Asunto(s)
Factor de Crecimiento Epidérmico , Factor A de Crecimiento Endotelial Vascular , Ratas , Animales , Factor de Crecimiento Epidérmico/metabolismo , Ciclooxigenasa 2 , Ratas Wistar , Cicatrización de Heridas , Fototerapia
4.
J Cosmet Dermatol ; 22(4): 1266-1272, 2023 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-36718803

RESUMEN

BACKGROUND: Currently, even individuals who do physical activity regularly have some degree of dissatisfaction with their own bodies. The electromagnetic field for supramaximal muscle contraction has been the subject of research. High-intensity supramaximal muscle stimulation (HI-SMS) is a non-invasive technology used to strengthen, firm, and tone the abdominal muscles, arms, buttocks, and thighs and has been indicated for aesthetic purposes. AIMS: The present study aimed to examine the safety and efficacy of HI-SMS used in the abdominal muscles of patients through the analysis of clinical evaluation, biochemical serum profile, and patient satisfaction with the procedure. PATIENTS/METHODS: This is retrospective non-randomized and non-controlled study collected in a private clinic; all data from healthy participants (n = 25), aged between 18 and 55 years, were compiled and analyzed. All received eight 30 min sessions of electromagnetic field ONIX HI-SMS (intensity of the 90%-100%) located in abdominal, twice a week with intervals of 2-3 days. RESULTS: The results show that BMI, fat thickness, and waist circumference improved the body contour after the treatment. There was no statistical difference in the data referring to the values of AST, ALT, ALP, creatinine, cholesterol, LDL-C, VLDL-C, HDL-C, glycemia, LDH, CK, and IL-6. However, there was a reduction of "non-esterified" free fatty acids when compared to baseline. This treatment provided high levels of tolerance, comfort, and high level of satisfaction. CONCLUSIONS: Thus, it can be suggested that the treatment with HI-SMS in abdominal muscles proves to be a safe technology with potential for non-invasive therapy for aesthetic purposes.


Asunto(s)
Músculos Abdominales , Magnetoterapia , Contracción Muscular , Satisfacción del Paciente , Adolescente , Adulto , Humanos , Persona de Mediana Edad , Adulto Joven , Brasil , Campos Electromagnéticos , Estudios Retrospectivos , Resultado del Tratamiento , Contracción Muscular/fisiología , Magnetoterapia/métodos , Músculos Abdominales/fisiología , Estética
5.
Lasers Med Sci ; 37(3): 1677-1686, 2022 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-34554354

RESUMEN

The purpose of this study is to evaluate the effects of photobiomodulation (PBM) therapy in chondrocyte response by in vitro experiments and cartilage repair using an experimental model of osteoarthritis (OA) in the knee of rats. The in vitro experiment was performed with chondrocyte cells, and they were divided into two groups: non-irradiated and irradiated with PBM (808 nm; 0.8 J or 1.4 J). Then, cell proliferation was evaluated after 1, 3, and 5 days. The experimental model of osteoarthritis (OA) was performed in the knee of 64 Wistar rats, and they were assorted into control group (CG), PBM (808 nm; 1.4 J). The results of in vitro showed that PBM 1.4 J increased cell proliferation, on days 1 and 5. However, after 3 days was demonstrated a significant increase in cell proliferation in PBM 0.8 J. The in vivo experiment results demonstrated, on histological analysis, that PBM presented less intense signs of tissue degradation with an initial surface discontinuity at the superficial zone and disorganization of the chondrocytes in the cartilage region when compared to CG, after 4 and 8 weeks. These findings were confirmed by immunohistochemistry and qRT-PCR analysis which showed that PBM increased IL-4, IL-10, COL-2, Aggrecan, and TGF-ß which are anabolic factors and acts on extracellular matrix. Also, PBM reduces the IL1-ß, an inflammatory marker that operates as a catabolic factor on articular cartilage. In conclusion, these results suggest that PBM may have led to a return to tissue homeostasis, promoting chondroprotective effects and stimulating the components of the articular tissue.


Asunto(s)
Cartílago Articular , Terapia por Luz de Baja Intensidad , Osteoartritis de la Rodilla , Osteoartritis , Animales , Cartílago Articular/patología , Condrocitos/patología , Modelos Animales de Enfermedad , Osteoartritis/genética , Osteoartritis/metabolismo , Osteoartritis/radioterapia , Osteoartritis de la Rodilla/genética , Osteoartritis de la Rodilla/radioterapia , Ratas , Ratas Wistar
6.
Lasers Med Sci ; 37(3): 1921-1929, 2022 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-34694503

RESUMEN

We are currently facing a pandemic that continuously causes high death rates and has negative economic and psychosocial impacts. Therefore, this period requires a quick search for viable procedures that can allow us to use safe and non-invasive clinical tools as prophylactic or even adjuvant methods in the treatment of COVID-19. Some evidence shows that photobiomodulation therapy (PBMT) can attenuate the inflammatory response and reduce respiratory disorders similar to acute lung injury (ALI), complications associated with infections, such as the one caused by the new Coronavirus (SARS-CoV-2). Hence, the aim of the present study was to evaluate the influence of PBMT (infrared low-level laser therapy) on the treatment of ALI, one of the main critical complications of COVID-19 infection, in an experimental model in rats. Twenty-four male Wistar rats were randomly allocated to three experimental groups (n = 8): control group (CG), controlled ALI (ALI), and acute lung injury and PBM (ALIP). For treatment, a laser equipment was used (808 nm; 30 mw; 1.68 J) applied at three sites (anterior region of the trachea and in the ventral regions of the thorax, bilaterally) in the period of 1 and 24 h after induction of ALI. For treatment evaluation, descriptive histopathological analysis, lung injury score, analysis of the number of inflammatory cells, and expression of interleukin 1 ß (IL-1ß) were performed. In the results, it was possible to observe that the treatment with PBMT reduced inflammatory infiltrates, thickening of the alveolar septum, and lung injury score when compared to the ALI group. In addition, PBMT showed lower immunoexpression of IL-1ß. Therefore, based on the results observed in the present study, it can be concluded that treatment with PBMT (infrared low-level laser therapy) was able to induce an adequate tissue response capable of modulating the signs of inflammatory process in ALI, one of the main complications of COVID-19.


Asunto(s)
COVID-19 , Terapia por Luz de Baja Intensidad , Animales , COVID-19/radioterapia , Terapia por Luz de Baja Intensidad/métodos , Pulmón/patología , Masculino , Ratas , Ratas Wistar , SARS-CoV-2
7.
Lasers Med Sci ; 37(2): 971-981, 2022 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-34041619

RESUMEN

The present study aimed to evaluate the new heterologous fibrin biopolymer associated, or not, with photobiomodulation therapy for application in tendon injuries, considered a serious and common orthopedic problem. Thus, 84 Rattus norvegicus had partial transection of the calcaneus tendon (PTCT) and were randomly divided into: control (CG); heterologous fibrin biopolymer (HFB); photobiomodulation (PBM); heterologous fibrin biopolymer + photobiomodulation (HFB + PBM). The animals received HFB immediately after PTCT, while PBM (660 nm, 40 mW, 0.23 J) started 24 h post injury and followed every 24 h for 7, 14, and 21 days. The results of the edema volume showed that after 24 h of PTCT, there was no statistical difference among the groups. After 7, 14, and 21 days, it was observed that the treatment groups were effective in reducing edema when compared to the control. The HFB had the highest edema volume reduction after 21 days of treatment. The treatment groups did not induce tissue necrosis or infections on the histopathological analysis. Tenocyte proliferation, granulation tissue, and collagen formation were observed in the PTCT area in the HFB and HFB + PBM groups, which culminated a better repair process when compared to the CG in the 3 experimental periods. Interestingly, the PBM group revealed, in histological analysis, major tendon injury after 7 days; however, in the periods of 14 and 21 days, the PBM had a better repair process compared to the CG. In the quantification of collagen, there was no statistical difference between the groups in the 3 experimental periods. The findings suggest that the HFB and PBM treatments, isolated or associated, were effective in reducing the volume of the edema, stimulating the repair process. However, the use of HFB alone was more effective in promoting the tendon repair process. Thus, the present study consolidates previous studies of tendon repair with this new HFB. Future clinical trials will be needed to validate this proposal.


Asunto(s)
Tendón Calcáneo , Calcáneo , Terapia por Luz de Baja Intensidad , Animales , Ratas , Biopolímeros , Fibrina , Ratas Wistar
8.
Lasers Med Sci ; 36(6): 1235-1240, 2021 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-33083912

RESUMEN

Acne vulgaris is the most recurring skin condition in the world, causing great harm to the physical and psychological well-being of many patients. Antimicrobial photodynamic therapy (aPDT) has broad therapeutic applicability. The purpose was to evaluate in vitro the photodynamic inactivation against Propionibacterium acnes (P. acnes) biofilms by using different concentrations of hypericin (Hypericum perforatum) photosensitizer associated with different energies of low-level laser. The biofilms were placed in 96-well microplates with a 6.4-mm diameter surface, by using standard suspensions (2 × 107 CFU/mL) and grown in brain heart infusion broth (BHI) for 48 h in anaerobic chamber. Subsequently, the control group received application of 0.9% sterile saline solution for 3 min; the photosensitising groups received hypericin at concentrations of 5 and 15 µg/mL for 3 min; the laser groups received irradiation of energies of 3 and 5 J (660 nm, continuous output, 100 mW, 30 and 50 s and 100 J/cm2 and 166 J/cm2, respectively); the aPDT groups received 5 and 15 µg/mL concentrations of hypericin associated with energies of 3 and 5 J of low-level laser irradiation. After the biofilms were broken up and seeded for CFU counting. The results showed a reduction in P. acnes biofilms after aPDT emphasising that 15 µg/mL hypericin associated with 3 and 5 J laser irradiation reduced biofilms by 14.1 and 27.9%, respectively. In addition, all groups of aPDT demostrated statistically significant reductions. In vitro photodynamic inactivation against P. acnes biofilms using different concentration of hypericin photosensitizer associated with different energies of low-level laser promoted effective antimicrobial action.


Asunto(s)
Fotoquimioterapia , Acné Vulgar/tratamiento farmacológico , Antracenos , Antibacterianos/uso terapéutico , Antiinfecciosos/uso terapéutico , Biopelículas/efectos de la radiación , Humanos , Hypericum , Rayos Láser , Luz , Perileno/análogos & derivados , Fotoquimioterapia/métodos , Fármacos Fotosensibilizantes/uso terapéutico , Propionibacterium acnes
9.
Am J Phys Med Rehabil ; 99(8): 725-732, 2020 08.
Artículo en Inglés | MEDLINE | ID: mdl-32167952

RESUMEN

OBJECTIVE: The present study aimed to evaluate the effectiveness of photobiomodulation therapy by light-emitting diode on osteoarthritis treatment in the knees of rats. DESIGN: Twenty male Wistar rats were randomly assigned into two experimental groups: OAC: animals subjected to induction of osteoarthritis, without therapeutic intervention and the group OAL: animals subjected to induction of osteoarthritis treated with light-emitting diode photobiomodulation therapy (850 nm, 200 mW, 6 J). RESULTS: The results of gait analysis showed no statistical difference between the groups. The histological findings showed that the OAL group presented abnormal chondrocyte orientation, yet with less irregularities along fibrillation and the joint tissue. Thus, it presented a lower degenerative process when evaluated by the Osteoarthritis Research Society International. Likewise, in the immunohistochemical analysis, the OAL group showed higher collagen 2 and transforming growth factor ß immunoexpression when compared with the OAC group. CONCLUSIONS: Given the above, it is possible to suggest that the photobiomodulation therapy by light-emitting diode had positive effects on the expression of extracellular matrix proteins responsible for synthesis of articular tissue.


Asunto(s)
Terapia por Luz de Baja Intensidad , Osteoartritis de la Rodilla/terapia , Animales , Condrocitos/patología , Colágeno Tipo II/metabolismo , Modelos Animales de Enfermedad , Análisis de la Marcha , Inmunohistoquímica , Osteoartritis de la Rodilla/patología , Ratas Wistar , Rodilla de Cuadrúpedos/metabolismo , Rodilla de Cuadrúpedos/patología , Factor de Crecimiento Transformador beta/metabolismo
10.
Lasers Med Sci ; 35(4): 939-947, 2020 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-31833005

RESUMEN

Skin graft is one of the most common techniques used in plastic surgery and repair. However, there are some complications that can lead to loss of the skin graft. Thus, several features have been studied with the aim of promoting the integration of skin grafts. Among these resources, the use of laser photobiomodulation (laser PBM) has been highlighted. The present study aimed to investigate the effects of laser PBM on the viability and integration of skin grafts in rats. Twenty male Wistar rats (± 250 g) were randomly assigned into two experimental groups with 10 animals each: control group, animals submitted to skin graft and simulation of laser PBM; laser PBM group, submitted to the skin graft and submitted to laser PBM at 660 nm, 40 mW, 60 s, 2.4 J. The animals were submitted to laser photobiomodulation immediately after the surgical procedure and each 24 h. Animal euthanasia occurred on the 7th day after surgery, 24 h after the last treatment session. The histopathological analysis revealed that the laser PBM showed better adhesion of the graft when compared to the control group. Likewise, the morphometric analysis of mast cells, blood vessels, and collagen showed a statistically significant increase in the animals irradiated with the laser PBM when compared to the control group. In addition, immunohistochemical analysis demonstrated that the laser PBM showed statistically higher immunoexpression of FGF when compared to the CG. However, IL-4 immunoexpression did not show statistical difference between the experimental groups. From the results obtained in the present study, it can be suggested that laser photobiomodulation was effective in promoting the integration and viability of total skin grafts in rats.


Asunto(s)
Terapia por Luz de Baja Intensidad , Trasplante de Piel , Animales , Recuento de Células , Colágeno/metabolismo , Interleucina-4/metabolismo , Masculino , Mastocitos/metabolismo , Ratas Wistar , Piel/irrigación sanguínea , Piel/patología
11.
Lasers Med Sci ; 35(1): 157-164, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31168678

RESUMEN

The aim of the present study was to evaluate the in vivo response of different wavelengths (red and near-infrared) of light-emitting diode (LED) on full-thickness skin grafts (FTSG) in rats. Thirty rats were randomly allocated into three experimental groups: control group (C); red LED treated group (R); and near-infrared LED group (NIR). Skin grafts were irradiated daily for ten consecutive days, starting immediately after the surgery using a red (630 nm) or near-infrared (850 nm) LED. The results showed that the red wavelength LED significantly enhanced the skin graft score in relation to the NIR group and increased transforming growth factor beta (TGF-ß) protein expression and density of collagen fibers compared with the other experimental groups. These results suggest that the red wavelength LED was efficient to improve the dermo-epidermal junction and modulate the expression proteins related to tissue repair.


Asunto(s)
Rayos Infrarrojos/uso terapéutico , Fototerapia/métodos , Trasplante de Piel , Animales , Colágeno/metabolismo , Epidermis/metabolismo , Epidermis/efectos de la radiación , Regulación de la Expresión Génica/efectos de la radiación , Masculino , Ratas , Factor de Crecimiento Transformador beta/metabolismo , Cicatrización de Heridas/fisiología , Cicatrización de Heridas/efectos de la radiación
12.
Braz. arch. biol. technol ; 63: e20180668, 2020. graf
Artículo en Inglés | LILACS | ID: biblio-1132159

RESUMEN

Abstract The aim of this study was to evaluate the in vivo response of red light-emitting diode (LED) on acute lung injury (ALI) in a sepsis model in rats. Twenty rats were randomly allocated into two experimental groups (n=10): Control Sepsis Group (CS); sepsis and red LED group (SRL). The anterior region of the trachea and ventral regions of the chest (below the ribs), bilaterally were irradiated daily for two consecutive days, starting immediately after the surgery using red (630 nm) LED. The histological results showed that in red LED treated group presented a modulation of the lung inflammatory process, less intense alveolar septum thickening and decrease of the inflammatory cells. Moreover, LED significantly reduced the lung injury score and increased interleukin type 10 (IL-10) protein expression compared SG. These results suggest that LED was efficient in attenuating ALI in a sepsis model in rats by reducing inflammatory cells into lung tissue and enhancing the anti-inflammatory cytokine production.


Asunto(s)
Animales , Masculino , Ratas , Sepsis/terapia , Terapia por Luz de Baja Intensidad , Láseres de Semiconductores , Lesión Pulmonar Aguda/terapia , Biomarcadores , Ratas Wistar , Modelos Animales de Enfermedad
13.
Braz. arch. biol. technol ; 63: e20180453, 2020. graf
Artículo en Inglés | LILACS | ID: biblio-1132256

RESUMEN

Abstract The aim of this study was to evaluate the effectiveness of 3 different fluences of low-level laser therapy (LLLT) in intermediate period of spinal cord repair using an experimental model in rats. Thirty two rats were randomly divided into four experimental groups: Control Group (CG); Laser treated group 500 J/cm² (L-500); Laser treated group 750 J/cm² (L-750) and Laser treated group 1000 J/cm² (L-1000). Spinal cord injury (SCI) was performed by an impactor equipment (between 9th and 10th thoracic vertebrae), with a pressure of 150 kdyn. Afterwards, the injured region was irradiated daily for 14 consecutive sessions, using an 808 nm laser, at the respective fluence of each experimental groups. Locomotor function and tactile sensitivity were performed on days 1 and 15 post-surgery. Animals were euthanized 15 days post-surgery and samples were retrieved for histological and immunohistochemistry analysis. Functional behavior and tactile sensitivity were improved after laser irradiation. Moreover, higher fluencies of LLLT reduced the volume of injury. Additionally, LLLT produced a decreased CD-68 expression. These results demonstrated that, for an intermediate period of SCI repair, LLLT at higher fluences, was effective in promoting functional recovery and modulating the inflammatory process in the spinal cord of rats after traumatic SCI.


Asunto(s)
Animales , Femenino , Ratas , Traumatismos de la Médula Espinal/terapia , Terapia por Luz de Baja Intensidad/métodos , Plasticidad Neuronal , Traumatismos de la Médula Espinal/patología , Inmunohistoquímica , Ratas Wistar , Modelos Animales de Enfermedad
14.
Lasers Med Sci ; 33(9): 1875-1882, 2018 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-29797102

RESUMEN

The aim of this study was to compare the effects of photobiomodulation (PBM) associated with an aerobic and an aquatic exercise training on the degenerative process related to osteoarthritis (OA) in the articular cartilage in rats. Fifty male Wistar rats were randomly divided into 5 groups: OA control group (CG), OA plus aerobic training group (AET), OA plus aquatic training group (AQT), OA plus aerobic training associated with PBM group (AETL), OA plus aquatic training associated with PBM group (AQTL). The aerobic training (treadmill; 16 m/min; 50 min/day) and the aquatic training (water jumping; 50-80% of their body mass) started 4 weeks after the surgery and they were performed 3 days/week for 8 weeks. Moreover, PBM was performed after the physical exercise trainings on the left joint. Morphological characteristics and immunoexpression of IL-10, TGF-ß, and collagen type I (Col I) and II (Col II) of the articular cartilage were evaluated. The results showed that all the treated groups (exercise and PBM) presented less intense signs of degradation (measured by histopathological analysis and OARSI grade system). Additionally, aerobic and aquatic exercise training rats (associated or not with PBM) showed increased IL-10 (AET p = 0.0452; AETL p = 0.03; AQTL p = 0.0193) and Col II (AET p = 0.012; AQT p = 0.0437; AETL p = 0.0001; AQTL p = 0.0001) protein expression compared to CG. Furthermore, a statistically higher TGF-ß expression was observed in AET (p = 0.0084) and AETL (p = 0.0076) compared to CG. These results suggest that PBM associated with aerobic and aquatic exercise training were effective in mediating chondroprotective effects and maintaining the integrity of the articular tissue in the knees of OA rats.


Asunto(s)
Colágeno Tipo II/metabolismo , Interleucina-10/metabolismo , Terapia por Luz de Baja Intensidad , Osteoartritis/metabolismo , Osteoartritis/radioterapia , Condicionamiento Físico Animal , Animales , Cartílago Articular/metabolismo , Cartílago Articular/patología , Cartílago Articular/efectos de la radiación , Colágeno Tipo I/metabolismo , Modelos Animales de Enfermedad , Humanos , Masculino , Osteoartritis/patología , Ratas Wistar , Factor de Crecimiento Transformador beta/metabolismo
15.
Lasers Med Sci ; 33(3): 549-557, 2018 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-29196833

RESUMEN

The aim of this study was to compare the effects of combined treatment with chondroitin sulfate and glucosamine sulfate (CS/Gl) and photobiomodulation (PBM) on the degenerative process related to osteoarthritis (OA) in the articular cartilage in rats. Forty male Wistar rats were randomly divided into four groups: OA control group (CG); OA animals submitted to PBM treatment (PBM); OA animals submitted to CS/Gl treatment (CS/Gl); OA submitted to CS/GS associated with PBM treatments (GS/Gl + PBM). The CS/Gl started 48 h after the surgery, and they were performed for 29 consecutive days. Moreover, PBM was performed after the CS/Gl administration on the left joint. Morphological characteristics and immunoexpression of interleukin 10 (IL-10) and 1 beta (IL-1ß) and collagen type II (Col II) of the articular cartilage were evaluated. The results showed that all treated groups (CS/Gl and PBM) presented attenuation signs of degenerative process (measured by histopathological analysis) and lower density chondrocytes [PBM (p = 0.0017); CS/Gl (p = 0.0153) and CS/Gl + PBM (p = 0.002)]. Additionally, CS/Gl [associated (p = 0.0089) or not with PBM (p = 0.0059)] showed significative lower values for OARSI grade evaluation. Furthermore, CS/GS + PBM decreased IL-1ß protein expression (p = 0.0359) and increased IL-10 (p = 0.028) and Col II imunoexpression (p = 0.0204) compared to CG. This study showed that CS/Gl associated with PBM was effective in modulating inflammatory process and preventing the articular tissue degradation in the knees OA rats.


Asunto(s)
Sulfatos de Condroitina/uso terapéutico , Glucosamina/uso terapéutico , Terapia por Luz de Baja Intensidad , Osteoartritis/tratamiento farmacológico , Osteoartritis/patología , Osteoartritis/radioterapia , Animales , Condrocitos/efectos de los fármacos , Condrocitos/patología , Condrocitos/efectos de la radiación , Sulfatos de Condroitina/farmacología , Colágeno Tipo II/metabolismo , Terapia Combinada , Modelos Animales de Enfermedad , Glucosamina/farmacología , Inmunohistoquímica , Interleucina-10/metabolismo , Interleucina-1beta/metabolismo , Masculino , Osteoartritis/metabolismo , Ratas Wistar
16.
Lasers Med Sci ; 32(2): 343-349, 2017 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-27909916

RESUMEN

The aim of this study was to evaluate the in vivo response of different fluences of low-level laser therapy (LLLT) on the area of the injury, inflammatory markers, and functional recovery using an experimental model of traumatic spinal cord injury (SCI). Thirty two rats were randomly divided into four experimental groups: control group (CG), laser-treated group 500 J/cm2 (L-500), laser-treated group 750 J/cm2 (L-750), and laser-treated group 1000 J/cm2 (L-1000). SCI was performed by an impactor equipment (between the ninth and tenth thoracic vertebrae), with a pressure of 150 kdyn. Afterwards, the injured region was irradiated daily for seven consecutive sessions, using an 808-nm laser, at the respective fluence of each experimental groups. Motor function and tactile sensitivity were performed on days 1 and 7 post-surgery. Animals were euthanized on the eighth day after injury, and the samples were retrieved for histological and immunohistochemistry analyses. Functional evaluation and tactile sensitivity were improved after LLLT, at the higher fluence. Additionally, LLLT, at 750 and 1000 J/cm2, reduces the lesion volume and modulates the inflammatory process with decrease of CD-68 protein expression. These results suggest that LLLT at higher doses was effective in promoting functional recovery and modulating inflammatory process in the spinal cord of rats after SCI.


Asunto(s)
Terapia por Luz de Baja Intensidad/métodos , Traumatismos de la Médula Espinal/radioterapia , Animales , Modelos Animales de Enfermedad , Femenino , Inmunohistoquímica , Actividad Motora , Ratas Wistar , Recuperación de la Función , Médula Espinal/patología , Traumatismos de la Médula Espinal/patología , Traumatismos de la Médula Espinal/fisiopatología , Tacto
17.
Lasers Med Sci ; 31(6): 1203-9, 2016 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-27250713

RESUMEN

The objective of the present study is to evaluate the acute effects of low-level laser therapy (LLLT) on functional capacity, perceived exertion, and blood lactate in hospitalized patients with heart failure (HF). Patients diagnosed with systolic HF (left ventricular ejection fraction <45 %) were randomized and allocated prospectively into two groups: placebo LLLT group (n = 10)-subjects who were submitted to placebo laser and active LLLT group (n = 10)-subjects who were submitted to active laser. The 6-min walk test (6MWT) was performed, and blood lactate was determined at rest (before LLLT application and 6MWT), immediately after the exercise test (time 0) and recovery (3, 6, and 30 min). A multi-diode LLLT cluster probe (DMC, São Carlos, Brazil) was used. Both groups increased 6MWT distance after active or placebo LLLT application compared to baseline values (p = 0.03 and p = 0.01, respectively); however, no difference was observed during intergroup comparison. The active LLLT group showed a significant reduction in the perceived exertion Borg (PEB) scale compared to the placebo LLLT group (p = 0.006). In addition, the group that received active LLLT showed no statistically significant difference for the blood lactate level through the times analyzed. The placebo LLLT group demonstrated a significant increase in blood lactate between the rest and recovery phase (p < 0.05). Acute effects of LLLT irradiation on skeletal musculature were not able to improve the functional capacity of hospitalized patients with HF, although it may favorably modulate blood lactate metabolism and reduce perceived muscle fatigue.


Asunto(s)
Lactatos/sangre , Terapia por Luz de Baja Intensidad/métodos , Fatiga Muscular/efectos de la radiación , Músculo Esquelético/efectos de la radiación , Adulto , Anciano , Método Doble Ciego , Prueba de Esfuerzo , Femenino , Insuficiencia Cardíaca/fisiopatología , Hospitalización , Humanos , Masculino , Persona de Mediana Edad , Proyectos Piloto
18.
Lasers Med Sci ; 31(6): 1219-29, 2016 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-27250715

RESUMEN

The aging process leads to a gradual loss of muscle mass and muscle performance, leading to a higher functional dependence. Within this context, many studies have demonstrated the benefits of a combination of physical exercise and low level laser therapy (LLLT) as an intervention that enhances muscle performance in young people and athletes. The aim of this study was to evaluate the effects of combination of LLLT and strength training on muscle performance in elderly women. For this, a hundred elderly women were screened, and 48 met all inclusion criteria to participate in this double-blind placebo-controlled trial. Volunteers were divided in three groups: control (CG = 15), strength training associated with placebo LLLT (TG = 17), and strength training associated with active LLLT (808 nm, 100 mW, 7 J) (TLG = 16). The strength training consisted of knee flexion-extension performed with 80 % of 1-repetition maximum (1-RM) during 8 weeks. Several outcomes related to muscle performance were analyzed through the 6-min walk test (6-MWT), isokinetic dynamometry, surface electromyography (SEMG), lactate concentration, and 1-RM. The results revealed that a higher work (p = 0.0162), peak torque (p = 0.0309), and power (p = 0.0223) were observed in TLG compared to CG. Furthermore, both trained groups increased the 1-RM load (TG vs CG: p = 0.0067 and TLG vs CG: p < 0.0001) and decreased the lactate concentration in the third minute after isokinetic protocol (CG vs TLG: p = 0.0289 and CG vs TG: p = 0.0085). No difference in 6-MWT and in fatigue levels were observed among the groups. The present findings suggested that LLLT in combination with strength training was able to improve muscle performance in elderly people.


Asunto(s)
Ejercicio Físico/fisiología , Terapia por Luz de Baja Intensidad/métodos , Músculo Esquelético/efectos de la radiación , Entrenamiento de Fuerza/métodos , Anciano , Método Doble Ciego , Electromiografía , Femenino , Humanos , Ácido Láctico/sangre , Persona de Mediana Edad , Músculo Esquelético/fisiología , Torque
19.
Connect Tissue Res ; 57(5): 398-407, 2016 09.
Artículo en Inglés | MEDLINE | ID: mdl-27220395

RESUMEN

OBJECTIVE: The aim of this study was to evaluate the effects of an aquatic exercise program and low-level laser therapy (LLLT) (associated or not) on degenerative modifications and inflammatory mediators on the articular cartilage using an experimental model of knee OA. METHOD: Forty male Wistar rats were divided into 4 groups: knee OA - without treatment (OA); OA plus exercise program group (OAE); OA plus LLLT (OAL); OA plus exercise program associated with LLLT (OAEL). Trained rats performed a water-jumping program carrying a load equivalent to 50-80 % of their body mass strapped to their chest. The laser irradiation was used either as the only method or after the exercise training had been performed, at 2 points contact mode (medial and lateral side of the left joint). The treatments started 4 weeks after the surgery, 3 days/week for 8 weeks. RESULTS: The results revealed that all treated groups (irradiated or not) exhibited a better pattern of tissue organization, with less fibrillation and irregularities along the articular surface and improved chondrocytes organization. Also, a lower cellular density and structural damage (OARSI score) and higher thickness values were observed in all treated groups. Additionally, OAE and OAEL showed a reduced expression in IL-1ß and caspase-3 as compared with OA. Furthermore, a statistically lower MMP-13 expression was only observed in OAEL as compared with OA. CONCLUSION: These results suggest that aquatic exercise program and LLLT were effective in preventing cartilage degeneration. Also, physical exercise program presented anti-inflammatory effects in the knees in OA rats.


Asunto(s)
Cartílago Articular/patología , Cartílago Articular/efectos de la radiación , Terapia por Luz de Baja Intensidad , Osteoartritis/radioterapia , Condicionamiento Físico Animal , Animales , Caspasa 3/metabolismo , Recuento de Células , Condrocitos/patología , Terapia Combinada , Modelos Animales de Enfermedad , Inmunohistoquímica , Masculino , Metaloproteinasa 3 de la Matriz/metabolismo , Osteoartritis/patología , Ratas Wistar , Resultado del Tratamiento
20.
Am J Phys Med Rehabil ; 95(7): 525-34, 2016 07.
Artículo en Inglés | MEDLINE | ID: mdl-26829073

RESUMEN

OBJECTIVE: The aim of this study was to evaluate the in vivo response of 2 different laser wavelengths (red and infrared) on skeletal muscle repair process in diabetic rats. DESIGN: Forty Wistar rats were randomly divided into 4 experimental groups: basal control-nondiabetic and muscle-injured animals without treatment (BC); diabetic muscle-injured without treatment (DC); diabetic muscle-injured, treated with red laser (DCR) and infrared laser (DCIR). The injured region was irradiated daily for 7 consecutive days, starting immediately after the injury using a red (660 nm) and an infrared (808 nm) laser. RESULTS: The histological results demonstrated in both treated groups (red and infrared wavelengths) a modulation of the inflammatory process and a better tissue organization located in the site of the injury. However, only infrared light significantly reduced the injured area and increased MyoD and myogenin protein expression. Moreover, both red and infrared light increased the expression of the proangiogenic vascular endothelial growth factor and reduced the cyclooxygenase 2 protein expression. CONCLUSION: These results suggest that low-level laser therapy was efficient in promoting skeletal muscle repair in diabetic rats. However, the effect of infrared wavelength was more pronounced by reducing the area of the injury and modulating the expression proteins related to the repair.


Asunto(s)
Rayos Infrarrojos/uso terapéutico , Terapia por Láser/métodos , Terapia por Luz de Baja Intensidad/métodos , Músculo Esquelético/lesiones , Músculo Esquelético/efectos de la radiación , Animales , Ciclooxigenasa 2/efectos de la radiación , Diabetes Mellitus Experimental/fisiopatología , Diabetes Mellitus Experimental/radioterapia , Proteína MioD/efectos de la radiación , Miogenina/efectos de la radiación , Ratas , Resultado del Tratamiento , Factor A de Crecimiento Endotelial Vascular/efectos de la radiación
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