Antioxidant, carbonic anhydrase inhibition and diuretic activity of Leptadenia pyrotechnica Forssk. Decne.

Background: Leptadenia pyrotechnica Forssk. Decne is a member of family Apocynaceae and locally known as 'Khipp'. It is found in dry, sandy habitat of Pakistan and in several other regions around the world including Asia, Tropical Africa, Western Gulf and Mediterranean countries. It has nutritional value, containing 4 % lipids, 23 % proteins, 28 % carbohydrates, 4 % fibers, vitamin E and several minerals. Traditionally, this plant has been used by several communities for pain, different inflammatory and kidney disorders. Ethno-botanical studies have reported the use of L. pyrotechnica in nephrolithiasis, kidney disorders and induction of diuresis, which requires a detailed pharmacological study to validate the folkloric use of L. pyrotechnica as diuretic. Methods: The 70 % methanolic L. pyrotechnica (Lp.Cr) extract was prepared and qualitatively checked for the presence of various phytochemicals. Phenolic, flavonoid, tannin and saponin contents were quantified. GC-MS analysis of Lp.Cr was also performed. Antioxidant potential of Lp.Cr was evaluated by DPPH, ABTS and nitrite radical scavenging assays. CUPRAC and FRAP assay described the reducing potential of Lp.Cr. Diuretic activity was performed in both acute and prolonged models at different doses followed by the estimation of electrolytes, urea and creatinine levels. The mechanism of diuresis was described by pre-treatment with atropine, l-NAME, indomethacin and carbonic anhydrase inhibition. Results: Lp.Cr. indicated high phenolic and flavonoid contents which correlated with good antioxidant activity. GC-MS analysis showed the presence of 104 compounds from different phytochemical classes. Diuretic activity was performed at 10-300 mg/kg concentrations where the dose of 100 and 300 mg/kg showed good diuretic and saluretic activity comparable to furosemide. Lp.Cr exhibited diuresis both in acute and prolonged study protocols which can be attributed to carbonic anhydrase inhibition, effect on prostaglandins and cholinergic pathways. Conclusion: L. pyrotechnica contained several phytochemicals and exhibited good antioxidant activity. It induced diuresis and saluretic activity which was comparable to furosemide at higher doses. Diuretic activity can be attributed to carbonic anhydrase inhibition, prostaglandin synthesis and cholinergic pathways.

Quercus floribunda Lindl. Ex A. Camus; a tremendous remedy against inflammation and associated symptoms.

Crude extracts prepared from aerial parts and nut galls of Quercus floribunda Lindl. Ex. A. Camus were evaluated for phytochemical screening, in vitro antioxidant, and in vivo analgesic, anti-inflammatory and antipyretic activities. Various solvents including methanol (M), acetone (A), distilled water (DW), distilled water + methanol (DWM) were used for extraction. Highest total phenolic (66.9 ± 0.05 µg GAE/mgE) and flavonoid content (38.4 ± 0.72 µg QE/mgE) were measured in QFAA extract by colorimetric methods. Cumulative maximum concentrations of polyphenols were quantified in QFMG, QFAA, and QFMA extracts i.e. 19.036, 15. 574 and 11.647 µg/mg of extract by RP-HPLC analysis. From aerial parts extracts, apentacyclic tritepenoid, glutinol was isolated using column chromatography techniques and structure was elucidated using spectroscopic techniques. QFDWMA (205.5 ± 0.56 µg AAE/mg of extract) showed highest total reducing power while highest total antioxidant capacity (207.1 ± 0.49 AAE/mg of extract) and free radical scavenging potential (96.1 ± 0.42%) were observed in QFAA extract. QFAA extract showed significant (p ≤ 0.001) analgesic potential in different pain models i.e. hot plate method, cold plate method, Haffner's tail clip method and acetic acid induced writhing assay having 50.20%, 62.07%, 57.26% and 70.49% analgesia respectively at 300 mg/kg. QFAA extract showed maximum anti-inflammatory activity in croton oil induced edema (68.83%) and in carrageenan induced paw edema models (72.32%) at 300 mg/kg concentration. QFAA extract markedly reduced the rectal temperature at 300 mg/kg concentration, in brewer's yeast induced pyrexia model. Detailed investigations can be executed in future to determine the molecular mechanisms of these pharmacological attributes.

Green synthesis of two-electron centre based ZnO/NiCo2S4 QDs-OVs using Punica granatum fruit peel extract for an exceptional visible light photocatalytic degradation of doxycycline and ciprofloxacin.

Biosynthesis of nanomaterials using plant extract makes them attractive in the field of photocatalysis as they are environmental friendly. The current study focused on the biosynthesis of ZnO/NiCo2S4 QDs (NCs) using Punica granatum fruit peel extract as the reducing agent. The nanomaterials were characterized with XRD, FTIR, Raman, SEM, TEM, UV-vis DRS, BET, PL, EIS, and ESR analysis and were used for photocatalytic degradation of doxycycline (DOX) and ciprofloxacin (CIP). The bandgap of ZnO is 3.2 eV, and the decoration of NiCo2S4 QDs aids in narrowing the bandgap (2.8 eV), making the NCs visible light active. The fabricated NCs achieved 99 and 89% degradation of DOX and CIP respectively. The photocatalytic efficiency of ZnO/NiCo2S4 QDs was much higher compared to individual ZnO and NiCo2S4 QDs. The half-life period of DOX and CIP were evaluated to be 58 and 152 min respectively. The percentage of TOC removal in the photodegraded product of DOX and CIP was estimated to be 99 and 89% respectively, indicating the mineralization of the compounds. The enhanced photocatalytic efficiency of the NCs was attributed to the narrowed visible light active bandgap, synergistic charge transfer across the interface, and lower charge recombination. The intermediates formed during the photocatalytic degradation of DOX and CIP were analyzed using GC-MS/MS analysis, and the photodegradation pathway was elucidated. Also, the toxicity of the intermediates was computationally analyzed using ECOSAR software. The fabricated ZnO/NiCo2S4 QDs have excellent stability and reusability, confirmed by XRD and XPS analysis. The reusable efficiency of the NCs for the photocatalytic degradation of DOX and CIP were 98.93, and 99.4% respectively. Thus, the biologically fabricated NCs are shown to be an excellent photocatalyst and have wide applications in environmental remediation.