RESUMEN
SUMMARY: Mormodica balsamina is a valuable medicinal plant that is used to treat wounds and inflammation; its leaves are also used as an antibiotic and in the treatment of stomach pain. This study was conducted to determine the anti-ulcer activity of methanolic leaf extract of Mormodica balsamina on ethanol-induced ulcer in albino rats. A total of 32 rats were used for the study. Groups I and II served as the baseline and negative controls respectively, while groups III-VII served as the test groups. Group I was untreated, while group II received 1ml/kg body weight of the vehicle (2 % DMSO). Three test groups (III - V) received methanol extracts (75 mg, 150 mg, 300 mg/kg body weight respectively) while the other three test groups (VI - VIII) received aqueous extracts (75 mg, 150mg, 300 mg/kg body weight respectively) via oral gavage for seven days prior to ulcer induction. The rats were sacrificed, stomachs excised and ulcers scored. Histological sections were produced and examined. Findings revealed that M. balsamina extracts protected the rats' gastric epithelia from ethanol induced ulceration to varying degree with the high dose (150 and 300 mg/kg) of both extracts offering the best preservation (42 % and 50 % ulcer protective index respectively) when compared to untreated animals. Histological findings correlated with calculated ulcer indices, with treated animals having less severe gastric mucosal lesions. In conclusion, extracts of M. balsamina may possess reasonable antiulcer activities in rats against ethanol induced gastric ulcer.
Mormodica balsamina es una valiosa planta medicinal que se utiliza para tratar heridas e inflamaciones; sus hojas también se utilizan como antibiótico y en el tratamiento del dolor de estómago. Este estudio se realizó para determinar la actividad antiulcerosa del extracto metanólico de hojas de Mormodica balsamina sobre la úlcera inducida por etanol en ratas albinas. Se utilizaron un total de 32 ratas para el estudio. Los grupos I y II sirvieron como referencia y controles negativos respectivamente, mientras que los grupos III-VII sirvieron como grupos de prueba. El grupo I no se trató, mientras que el grupo II recibió 1 ml/kg de peso corporal del vehículo (2% de DMSO). Tres grupos de prueba (III - V) recibieron extractos de metanol (75 mg, 150 mg, 300 mg/ kg de peso corporal respectivamente) mientras que los otros tres grupos de prueba (VI - VIII) recibieron extractos acuosos (75 mg, 150 mg, 300 mg/kg de peso corporal respectivamente) por sonda oral durante siete días antes de la inducción de la úlcera. Se sacrificaron las ratas, se extirparon los estómagos y se puntuaron las úlceras. Se realizaron y examinaron secciones histológicas. Los resultados revelaron que los extractos de M. balsamina protegieron el epitelio gástrico de las ratas de la ulceración inducida por etanol en diversos grados, y la dosis alta (150 y 300 mg/kg) de ambos extractos ofreció la mejor conservación (42 % y 50 % de índice de protección contra úlceras, respectivamente) en comparación con los animales no tratados. Los hallazgos histológicos se correlacionaron con los índices de úlcera calculados, y los animales tratados tenían lesiones de la mucosa gástrica menos graves. En extractos de M. balsamina puede poseer actividades antiulcerosas razonables en ratas contra la úlcera gástrica inducida por etanol.
Asunto(s)
Animales , Ratas , Úlcera Gástrica/tratamiento farmacológico , Extractos Vegetales/administración & dosificación , Momordica/química , Etanol/toxicidad , Antiulcerosos/administración & dosificación , Plantas Medicinales , Úlcera Gástrica/inducido químicamente , Extractos Vegetales/química , Momordica balsamica , Hojas de la Planta , Modelos Animales de Enfermedad , Mucosa Gástrica/efectos de los fármacos , Antiulcerosos/químicaRESUMEN
The objective of the present study was to explore on the possible protective effects of the saponins and alkaloid-rich fractions of Colocasia esculenta L. Schott leaves on high-fat diet-induced changes in rats. High-fat diet (HFD)-fed rats were treated orally with 10mg/kg b.wt of saponin-rich fraction [SPF], 10mg/kg bw of alkaloid-rich fraction [ALF] and 400mg/kg bw of Crude aqueous extract of C. esculenta [CEAE] for 28 days. The effects of the treatments on body weight, wet white adipose (WAT) tissue, liver marker enzymes and liver histomorphology were studied. High-fat diet induced body weight gain and increased the serum levels of liver enzymes, cholesterol and triglycerides in rats. Increased body weight changes were observed in HFD-control while the alkaloid and CEAE treatments significantly decreased the weight gain of treated rats. Oral treatment with CEAE and fractions significantly decreased all the biochemical parameters (p<0.05) except cholesterol levels. Microscopical examination of the WAT showed decreased adipocyte sizes upon treatments with CEAE and fractions while the liver histoarchitecture showed a better preservation upon treatments with SPF and CEAE. The outcomes from the present study suggest that C. esculenta leaves significantly reduced fat accumulation in adipose tissues and ameliorated HFD-induced liver damage. The alkaloids and saponins present in the plant product may be the phytoconstituents responsible for the weight attenuating and hepatoprotection activities respectively.