Yeast cell wall and live yeast products and their combination in broiler diets formulated with weekly ingredient variations.

A 6-week broiler study was conducted to evaluate whether subjecting the intestinal microflora of broilers to the effect of weekly variations in feed ingredients could be ameliorated by the inclusion of yeast-derived feed additives: a yeast cell wall extract (YCW), live yeast culture (LY) or their combination (YCW + LY). Recent changes in ingredient prices have motivated producers to formulate diets not necessarily based primarily on corn and soya bean meal. Intestinal microflora in birds can vary significantly based on the ingredient composition of their diet, and the make-up of the flora can influence overall bird performance. Within the three nutrient phases of this study, birds were fed either a traditional corn-soya ingredient profile or a variable-ingredient regimen, which had weekly changes in the ingredient composition. There were consistent ameliorative effects of the yeast treatments in both the corn-soya and the variable-ingredient groups throughout all 6 weeks, with the YCW + LY combination showing a reduced effect when compared to either product fed alone. The effectiveness of YCW and LY on ameliorating the effects of weekly ingredient variations appeared most effective during the starter and grower phases, but was less significant during the sixth week.

Antiparasitic effect of wild rue (Peganum harmala L.) against experimentally induced coccidiosis in broiler chicks.

Organic farming of poultry has increased in recent years as the prophylactic use of antibiotics has come into disfavor. This study was conducted to explore the antiparasitic effect of a methanolic extract of Peganum harmala in broilers challenged with coccidiosis. For this purpose, 200 1-week-old broiler chicks were divided into five treatments: negative control (basal diet, Ph-0/NC), positive control (basal diet with coccidiosis challenge, Ph-0/C), and three groups challenged with coccidiosis and supplemented with P. harmala at the rate of 200 mg L(-1) (Ph-200), 250 mg L(-1) (Ph-250), and 300 mg L(-1) (Ph-300) drinking water. Each group had three replicates of ten chicks each. Challenge with standard dose of the larvae of coccidiosis and supplementation of P. harmala were initiated on day 14 until 35 days of age. As expected, the results revealed that weight gain, feed intake, and feed conversion ratio (FCR) were depressed significantly in Ph-0 group with significant mortality percentage. Weight gain, total body weight, and FCR increased linearly with increasing dose of P. harmala with the exception of feed intake. The growth and feed efficiency of Ph-0/NC was better in Ph-0/NC compared to that in Ph-0/C and comparable to that in P. harmala-treated birds. Similarly, mean ooccytes per gram (OPG) decreased linearly (P < 0.05) in supplemented groups compared to that in Ph-0/C. Histological evidences showed that cecal lesion and leucocyte infiltration decreased markedly in supplemented groups of P. harmala specifically the Ph-300 group compared to those in Ph-0/C. From the present experiment, we concluded the anticoccidial effect of P. harmala in broiler chicks.

Soybean, palm kernel, and animal-vegetable oils and vitamin E supplementation effect on lipid oxidation stability of sous vide chicken meat.

There is an increasing demand in precooked chicken meat products for restaurants and catering services. Because cooked chicken meat develops lipid oxidation relatively fast, sous vide chicken meat was studied to assess its shelf-life. Six hundred Cobb x Ross broilers were fed for 6 wk with a basal corn-soybean meal diet including soybean, palm kernel, or animal-vegetable oil, each supplemented with 33 or 200 mg/kg of dl-alpha-tocopheryl acetate. Broilers were randomly assigned into 6 treatments and 4 repetitions with 25 birds each. Boneless breast or thigh muscle pieces were dissected into 5 x 5 x 5 cm cubes, vacuum-packed, cooked in water bath (until 74 degrees C internal temperature), chilled, and stored at 4 degrees C for 1, 5, 10, 25, and 40 d. For each storage day, each pouch contained 3 pieces of meat, either breast or thigh. Thiobarbituric acid reactive substances analysis, to quantify malonaldehyde (MDA) values, was conducted to estimate the lipid oxidation development. Nonheme iron values of cooked meat were analyzed. Fatty acid methyl esters analysis was performed in chicken muscle to determine its fatty acid composition. There was no interaction between dietary fat and vitamin E level in all of the variables studied except in nonheme iron. Dietary fat significantly influenced the fatty acid composition of the muscle (P < 0.01), but it did not affect the MDA values, regardless of differences in the muscle fatty acid composition between treatments. Supplementation of the high level of vitamin E significantly reduced the MDA values in both breast and thigh meat (P < 0.01). The maximum MDA values were observed at d 40 of storage in thigh and breast meat in animal-vegetable and soybean oil treatments with the low levels of vitamin E, 0.91 and 0.70 mg/kg, respectively. Nonheme iron values in thigh meat differed between treatments at 1 or 25 d of storage but not in breast meat. In conclusion, refrigerated sous vide chicken meat has a prolonged shelf-life, which is enhanced by dietary supranutritional supplementation of vitamin E.

Effects of guar meal by-product with and without beta-mannanase Hemicell on broiler performance.

Guar gum production yields a high protein guar meal that can be subdivided into germ and hull fractions. Feeding high concentrations of guar meal reduces body weight and feed efficiency in chickens due to the presence of a residual guar gum. Two experiments determined the upper feeding levels of guar meal and the hull and germ fractions in broiler chickens. An industrial source beta-mannanase (Hemicell) also was fed in combination with guar meals. Experiment 1 utilized a 3 x 4 factorial design to feed broiler chickens diets containing guar germ, guar hull, or guar meal at 4 levels (2.5, 5.0, 7.5, and 10.0%) compared with a negative control diet. Results indicated that any of the 3 guar meals could be fed at a 2.5% dietary inclusion rate without adversely affecting broiler chicken growth to 6 wk of age. In experiment 2, a 4 x 2 factorial design consisting of the 3 by-products meals at 5% inclusion and soybean meal control with and without enzyme tested whether Hemicell could increase inclusion rates without decreasing broiler growth or feed consumption to 6 wk of age. Addition of Hemicell to feed had no effect on measures of growth in chickens fed the control diet. Hemicell significantly improved feed:gain ratio of diets containing 5% of each fraction of guar meal versus the untreated diets. Feed:gain ratio for the Hemicell-treated 5% germ fraction diet was improved to control diet levels. Results indicated that the upper feeding level of guar meal and germ and hull fraction of guar meal is 2.5%, and addition of beta-mannanase (Hemicell) increases the upper feeding level for the germ fraction to 5%.

Tissue distribution of gossypol enantiomers in broilers fed various cottonseed meals.

Plasma and tissue concentrations of total, (+)- and (-)-gossypol were determined in broilers fed cottonseed meals (CSM) from eight oil mills (five expander solvent, two expeller, and one direct solvent). Free gossypol in the meals ranged from 0.033 to 0.180%, and total gossypol ranged from 0.974 to 1.459%. The (+)-enantiomer of gossypol varied from 53.8 to 61.3% of total gossypol. Eight CSM diets containing 28% CSM and a soybean meal control diet were fed to 162 1-d-old male broiler chicks during a 3-wk starter period. Concentrations of free gossypol in the CSM diets ranged from 92 to 504 microg/g, and total gossypol ranged from 2,626 to 4,085 microg/g. All diets were formulated with the same concentrations of digestible lysine and methionine and were fed ad libitum. At 21 d of age, there were no significant differences in body weights, feed conversions, or mortality of birds fed the CSM diets when compared to birds fed the soybean meal diet. Concentrations of (+)- and (-)-gossypol in plasma, liver, kidney, and muscle increased linearly as the level of free gossypol increased in the diets. Liver had the highest concentration of total gossypol (71.4 to 313.6 microg/g DM) followed by kidney (9.2 to 36.3 microg/g DM), plasma (3.0 to 14.6 microg/mL), and muscle (2.1 to 9.8 microg/g DM). The proportion of (-)-gossypol was higher in plasma (26.7%) and kidney (25.6%) than in muscle (19.1%) and liver (16.0%). Performance data from this study indicate CSM can be used successfully in chick starter diets at levels up to 28% when diets are formulated on a digestible amino acid basis.

Use of expander cottonseed meal in broiler diets formulated on a digestible amino acid basis.

This experiment was designed to evaluate the use of expander cottonseed meal (CSM) in broiler diets formulated on a digestible amino acid basis and to determine the tissue distribution of gossypol enantiomers and their relationship to gossypol consumption. Gossypol is an antinutritional polyphenolic pigment found in cottonseed. A total of 800 1-d-old broilers (Arbor Acres) was randomly divided into 40 groups of 20 birds each and placed in a broiler house with wood shavings litter. The CSM was included in starter and grower diets at 0, 7, 14, 21, and 28% of the diet. Diets were formulated to have similar levels of digestible methionine and lysine as the 0% CSM control diet. At 21 d of age, five birds per pen were randomly selected for the determination of gossypol enantiomers in plasma, liver, heart, and breast muscle. Tissues were again collected at 42 d of age. Results of this experiment indicated that at 21 d of age cumulative body weight and feed-to-BW ratios of birds fed CSM diets were not significantly different from the control. By 35 d of age, feed-to-BW ratios of experimental birds fed the 28% CSM diet were significantly greater than the control birds, and by 42 d, BW were lower than those of the control group. Tissue concentrations of total, (+) and (-) gossypol increased linearly as the level of CSM increased. At 42 d, liver had the most gossypol with a ratio of 87% (+) gossypol to 13% (-) gossypol. Plasma contained 73% (+) gossypol and 27% (-) gossypol. Heart contained 45% (+) gossypol and 55% (-) gossypol. Breast muscle had the least gossypol. Results of this experiment indicated that expander solvent CSM could be fed to broilers at up to 21% of the total diet if amino acid digestibility was considered.

Cottonseed with a high (+)- to (-)-gossypol enantiomer ratio favorable to broiler production.

This study was designed to evaluate the relative toxicity of (+)- and (-)-gossypol enantiomers in 0-3-week-old broilers. Treatments consisted of broiler starter diets formulated with either a glandless, which did not contain gossypol, a commercial glanded [62. 2% (+)-gossypol], or a glanded moco [83.2% (+)-gossypol] crushed cottonseed (CCS) (six replicates/treatment) plus a soybean meal negative control. Glandless cottonseed was mixed with the moco cottonseed (2.4% free gossypol) so that both the commercial glanded and moco glanded cottonseeds contained equivalent concentrations of free gossypol (2.0%). The cottonseed treatments were added at 5 and 10% of the diet. Body weights and feed conversions were determined weekly. Body weights and feed-to-gain ratios of broilers fed 5 and 10% glandless CCS and 5% moco CCS were not significantly different. Broilers receiving 10% commercial glanded CCS weighed significantly less than those subjected to all other treatments. Feed-to-gain ratios were significantly higher for broilers receiving 10% commercial glanded and 10% moco CCS as compared to 5% moco and glandless CCS, 10% glandless CCS, and control. Relative liver weights of birds receiving 10% moco CCS were significantly less than those of birds receiving 10% commercial CCS. The data clearly showed that broilers fed moco CCS containing a relatively high (+)- to (-)-gossypol enantiomer ratio performed better than broilers receiving commercial CCS with a lower (+)- to (-)-gossypol enantiomer ratio.

Effect of beta-carotene, canthaxanthin, lutein, and vitamin E on neonatal immunity of chicks when supplemented in the broiler breeder diets.

The study was designed to assess neonatal immunity of chicks hatched from breeders fed diets supplemented with beta-carotene, canthaxanthin, lutein, or vitamin E. Broiler breeder birds were fed experimental diets consisting of control, 0.04% beta-carotene, 0.04% canthaxanthin, 0.04% lutein, 0.03% alpha-tocopherol acetate, or 0.04% beta-carotene plus 0.03% alpha-tocopherol acetate. Three weeks after initiation of experimental feeding, birds were vaccinated against Newcastle disease virus. Chicks hatched from the eggs of these breeders were used to determine the neonatal immune responses. There were no significant differences in weight gain and antibody titers of 3-wk-old chicks. 3H-Thymidine uptake by bursal lymphocytes when stimulated with tetrahydrofuran was significantly higher for the chicks hatched from breeders fed diets supplemented with vitamin E, or vitamin E plus beta-carotene, than in controls. 3H-Thymidine uptake by splenic lymphocytes when stimulated with concanavalin A and phorbol 12-myristate 13 acetate was significantly higher for the chicks hatched from breeders fed diets supplemented with vitamin E or beta-carotene alone, or vitamin E plus beta-carotene, than for the control chicks. Chicks hatched from hens supplemented with vitamin E had significantly higher antibody titers at 1 and 7 d of age than chicks from the control group. Vitamin E supplementation of breeder birds increased the immune response of their progeny.

Neonatal immune response and growth performance of chicks hatched from single comb White Leghorn breeders fed diets supplemented with beta-carotene, canthaxanthin, or lutein.

Forty Single Comb White Leghorn (SCWL) hens and 8 SCWL cocks were randomly divided into four treatment groups. Each group was fed a diet containing .02% beta-carotene, canthaxanthin, lutein, or basal control. After 20 d of feeding, eggs were collected daily from each experimental group for incubation. Two different hatches were set and chicks from each hatch were used for one of two different experiments. In both experiments, 24 chicks per treatment were vaccinated against Newcastle disease virus at 1 d of age and raised for 5 wk on a basal diet. In the second experiment, birds were revaccinated at 3 wk of age. In both experiments, at the end of 5 wk birds were killed and bursa of Fabricius, liver, and spleen were collected. For both experiments, there were no differences in antibody titers, weight gain, feed conversion ratio, and relative bursa weights of chicks. However in the second experiment, birds hatched from breeders fed lutein had significantly lower relative liver weights than chicks of the other treatments, whereas birds hatched from the breeders fed beta-carotene and canthaxanthin had significantly lower spleen weights than the control. These experiments suggest that carotenoids may not be effective in increasing neonatal immune response when they supplement practical breeder diets.

alpha-tocopherol, beta-carotene, and retinol enrichment of chicken eggs.

Due to the numerous health benefits associated with consumption of antioxidants such as alpha-tocopherol and beta-carotene, an experiment was conducted to determine dietary levels that would significantly enhance their concentration in chicken egg yolks. In the experiment, 127 Single Comb White Leghorn laying hens were divided into treatment groups (n = 40 per treatment group) and fed diets containing 0, 50, 100, 200, or 400 mg/kg beta-carotene, dl-alpha-tocopheryl acetate, or their combination. Yolk alpha-tocopherol increased (P < .05) from the control level of 144 micrograms/g of yolk to 477 micrograms/g of yolk when 400 mg dl-alpha-tocopheryl acetate/kg of diet was supplemented. Yolk retinol levels increased (P < .05) from 11.6 micrograms/g of yolk in controls to 13.9 micrograms/g of yolk at 200 mg beta-carotene/kg of diet. beta-Carotene content in the yolk also increased (P < .05) from .14 micrograms/g of yolk in controls to 5.19 micrograms/g of yolk at 200 mg beta-carotene/kg of diet. Supplemental beta-carotene markedly decreased the yolk deposition of alpha-tocopherol when the two compounds were fed together. Egg production, egg weight, and egg yield were not affected by dietary supplementations. Although the data indicated that it is possible to significantly increase the concentration of all three compounds in chicken eggs, because of the relative expense involved it may not be commercially viable to increase egg yolk concentrations of beta-carotene or retinol by supplementing beta-carotene in the diet.

An approach to training and retaining primary care physicians in rural Appalachia.

The West Virginia School of Osteopathic Medicine (WVSOM) educated and retained more primary care physicians for practice in rural Appalachia than did any other U.S. medical school from 1978 through 1990. This article describes the most important methods used at WVSOM to place physicians in rural areas: (1) The school has a focused, achievable mission (to provide primary care physicians who are trained to meet the medical needs of rural Appalachia and to improve the health care of the rural Appalachian population) that is agreed upon by the administration, faculty, and students; (2) it participates in a multistate educational exchange program with a similar mission; (3) it emphasizes personalized and interactive recruiting, admission, and placement processes aimed to attract nontraditional, rural students; (4) it provides early and long-term clinical training in rural sites (both hospitals and physicians' offices); (5) it is dedicated primarily to the education of medical students rather than to research or other goals; and (6) it is a freestanding school in a rural environment. The authors state that although WVSOM is unusual in some respects, at least some of its methods may be useful to other medical schools as they seek to produce more primary care physicians for rural and other underserved areas.

Impact of L-phenylalanine supplementation on the performance of three-week-old broilers fed diets containing ochratoxin A. 1. Effects on body weight, feed conversion, relative organ weight, and mortality.

An experiment with a completely randomized 2-by-3 factorial design was used to study the effects of ochratoxin A (OA; 0 and 4 mg of OA per kg) and supplemental L-phenylalanine (Phe; .0%, .8%, and 2.4% of Phe) in the diets of 3-wk-old broilers. Diets based on ground yellow corn and dehulled soybean meal were fed from Day 1 to 3 wk of age. A total of 240 male Hubbard-by-Hubbard broilers were randomly placed in battery brooders with 10 birds per pen. Each treatment was replicated 4 times. The parameters measured included mortality, BW, feed conversion, and relative organ weight. Broilers receiving OA weighed less and had poorer feed conversions than birds not receiving OA. For broilers receiving OA, the relative weights (grams of organ weight per 100 g of BW) of the liver, proventriculus, gizzard, and heart increased, while the relative weight of the bursa decreased. Supplemental Phe decreased the relative weight of the liver and increased the relative weight of the gizzard and heart. The regression slopes for Phe at 4 mg of OA per kg of diet were significantly different from 0 for BW, the relative weights of the kidney, spleen, and pancreas and approached significance for mortality (P = .065). In the absence of supplemental Phe, 42.5% of the birds died during the study when the dose level was 4 mg of OA per kg of diet. However, when Phe was supplemented at .8 and 2.4%, only 12.5 and 15.0% of the birds died, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Impact of L-phenylalanine supplementation on the performance of three-week-old broilers fed diets containing ochratoxin A. 2. Effects on hematology and clinical chemistry.

Phenylalanine was evaluated for its ability to protect broiler chickens from the toxic effects of ochratoxin A (OA). A completely randomized 2-by-3 factorial design was utilized consisting of 0, .8, and 2.4% supplemental L-phenylalanine (Phe) and of 0 and 4 mg of OA per kg of diet. The basal diet contained 14% protein. Broilers were raised in battery brooders to 3 wk of age, when blood was collected and various hematological parameters were determined. The health status of the broilers was evaluated by assaying serum for various enzyme activities and metabolites using an automated, clinical chemistry analyzer. Adding OA to the broiler diets resulted in an increased concentration of serum hemoglobin as well as increased activity for cholinesterase and gamma glutamyl transferase but in decreased activity for aspartate amino transferase, lactate dehydrogenase, and alkaline-phosphatase activity as well as decreased concentrations of total triglyceride and of inorganic phosphorus. Supplemental Phe decreased the concentrations of hemoglobin and serum glucose. The regression slopes for Phe at 4 mg of OA per kg of diet were significant for uric acid, creatinine, total protein, albumin, and cholesterol suggesting that supplemental Phe improved the health status of the broilers fed diets containing OA with respect to these parameters.

Effects of graded levels of dietary phosphorus on bone mineralization in the very young poult.

An experiment was conducted to determine the effects of graded levels of dicalcium phosphate on bone mineralization in 1 and 3-week-old poults. Corn-soybean meal diets calculated to contain 1.5% calcium and either .35, .55, .75, .95, or 1.15% available phosphorus were fed to battery brooder reared poults for 3 weeks. Body weights and percent tibia ash were determined at 1 and 3 weeks. Body weight was not significantly affected by dietary phosphorus at either 1 or 3 weeks of age. Percent tibia ash was significantly greater in 1-week-old poults receiving a minimum of .75% available phosphorus. In 3-week-old poults, maximum tibia ash was obtained with with a minimum of .55 available phosphorus. This study suggests the level of dietary phosphorus necessary to achieve maximum tibia ash in 3-week-old poults is not sufficient for maximum tibia ash in 1-week-old poults.

Effects of brooding temperature and molybdenum on the turkey poult.

Day-old White Broad Breastfed male turkeys were brooded for 10 days in a Honeywell environmental chamber. The chamber was configured to brood one-half of the birds at 29 C and the other half at 35 C. The poults were fed a practical corn-soy starter diet containing .8% phosphorus and 1.35% calcium. A 5 ppm molybdenum supplement was given to half the birds at each temperature. A significant (P less than or equal to .05) temperature X diet interaction occurred with both bone ash and serum phosphorus. Bone ash and serum phosphorus were significantly lower (P less than or equal to .05) in poults fed the diet supplemented with 5 ppm molybdenum at 29 C. Molybdenum supplementation did not significantly affect bone ash at 35 C but significantly increased (P less than or equal to .05) serum phosphorus at this brooding temperature. Both body weight and serum calcium were significantly higher (P less than or equal to .05) in poults brooded at 35 versus 29 C. Diets containing the molybdenum supplement did not significantly affect either body weight or serum calcium.

25-hydroxycholecalciferol-1-hydroxylase activity in heat stressed laying hens.

The activity of 25-hydroxycholecalciferol-1-hydroxylase was assayed in heat stressed laying hens fed a 3.5% calcium diet with either .625% or .925% phosphorus. The activity of this enzyme in the heat stressed birds was higher than in the control birds (P less than or equal to .07). The phosphorus content of the diets had no effect on enzyme activity.