RESUMEN
Application of substances from medicinal mushrooms is one of the interesting approaches to improve cancer therapy. In this study, we commenced a new attempt in the field of Heterobasidion annosum (Fr.) Bref. sensu lato to further extend our knowledge on this basidiomycete fungus. For this purpose, analysis of the active substances of Heterobasidion annosum methanolic extract and also its influence on colorectal cancer in terms of in vitro and in vivo experiments were performed. In vivo studies on mice were conducted to verify its acute toxicity and to further affirm its anticancer potential. Results indicated that all the most common substances of best known medicinal mushrooms that are also responsible for their biological activity are present in tested extracts. In vitro tests showed a high hemocompatibility and a significant decrease in viability and proliferation of DLD-1 cells in a concentration-dependent manner of Heterobasidion annosum extract. The studies performed on xenograft model of mice showed lower tendency of tumor growth in the group of mice receiving Heterobasidion annosum extract as well as mild or moderate toxicity. Obtained results suggest beneficial potential of Heterobasidion annosum against colon cancer as cytotoxic agent or as adjuvant anticancer therapy.
Asunto(s)
Basidiomycota/química , Proliferación Celular/efectos de los fármacos , Neoplasias del Colon/tratamiento farmacológico , Extractos Vegetales/farmacología , Animales , Apoptosis/efectos de los fármacos , Neoplasias del Colon/patología , Humanos , Ratones , Extractos Vegetales/química , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
Mushrooms have been widely used in traditional medicine for the treatment of various diseases. Today, their therapeutic value is scientifically studied and appreciated. Research indicates that polypores - a large group of fungi of the phylum Basdioinycota - exhibit antiviral, antimicrobial, anticancer, anti-allergic, anti-atherogenic, hypoglycemic, hepatoprotective and anti-inflammatory activities. Phellinus igniarius, a polypore mushroom, is one of the most used in traditional Asian medicine. Its potent anticancer activity has been repeatedly reported. In the past two decades, numerous pharmacologically active metabolites have been isolated and identified from P. igniarius. Among the large number of compounds, the most active group are polysaccharides. They modulate immune responses and inhibit tumor growth.
Asunto(s)
Agaricales/química , Factores Biológicos/química , Estructura Molecular , PoloniaRESUMEN
In this work, ether extracts of propolises from 11 countries of Europe and Asia together with extracts of the buds of their principal plant precursors were prepared and investigated by gas chromatography-mass spectrometry. For the first time, chemical compositions of the exudates of aspen, white birch and silver birch buds were determined. Basing on the data on the content of individual components and their groups, the method of rapid examination of plant precursors of propolis was developed. This new method is based on the use of a chromatogram for one extracted ion m/z=219, characteristic for a group of similar substances: phenylpropenoids. The I(T) retention indices of silylated derivatives of 449 substances registered in the buds of 6 species of trees and 37 samples of propolis extracts were calculated. These useful analytical parameters (majority of which are absent in the available databases) are attached in Supplementary information.