RESUMEN
BACKGROUND: This study evaluated the effects of C. verbenacea essential oil topically administered in a rat periodontitis model. METHODS: Periodontitis was induced on rats in one of the mandibular first molars assigned to receive a ligature. Animals were randomly divided into two groups: a) non-treatment group (NT) (n = 18): animals received 1mL of vehicle; b) C. verbenacea group (C.v.) (n = 18): animals received 5mg/Kg of essential oils isolated from C. verbenacea. The therapies were administered topically 3 times daily for 11 days. Then, the specimens were processed for morphometric analysis of bone loss. The ligatures were used for microbiological assessment of the presence of Aggregatibacter actinomycetemcomitans, Tannerella forsythia and Porphyromonas gingivalis using PCR. The gingival tissue was collected to Elisa assay of interleukin (IL)-1α and IL-10 levels. RESULTS: Bone loss was inhibited by C. verbenacea when compared to the NT group (p < 0.05). A decrease in the levels of IL-1α and increase in the IL-10 amounts was observed in the C.v. group as compared to NT group (p < 0.05). A lower frequency of P. gingivalis was found in C.v. group (p < 0.05). CONCLUSION: C. verbenacea essential oil topically administered diminished alveolar bone resorption, promoting a positive local imbalance in the pro/anti-inflammatory system and reducing the frequency of detection of P. gingivalis.
Asunto(s)
Antibacterianos/uso terapéutico , Antiinflamatorios/uso terapéutico , Resorción Ósea/prevención & control , Cordia , Periodontitis/tratamiento farmacológico , Periodoncio/efectos de los fármacos , Fitoterapia , Administración Tópica , Animales , Antibacterianos/farmacología , Antiinflamatorios/farmacología , Resorción Ósea/etiología , Resorción Ósea/metabolismo , Resorción Ósea/microbiología , Huesos/efectos de los fármacos , Modelos Animales de Enfermedad , Encía/efectos de los fármacos , Encía/metabolismo , Encía/microbiología , Interleucina-10/metabolismo , Interleucina-1alfa/metabolismo , Masculino , Diente Molar , Aceites Volátiles/farmacología , Aceites Volátiles/uso terapéutico , Periodontitis/complicaciones , Periodontitis/metabolismo , Periodontitis/microbiología , Periodoncio/microbiología , Extractos Vegetales/farmacología , Extractos Vegetales/uso terapéutico , Porphyromonas gingivalis/efectos de los fármacos , Ratas , Ratas WistarRESUMEN
The aim of this study was to evaluate the effect of an organic extract obtained from Ipomoea alba L. (Convolvulaceae or OE 1493), on experimental periodontal disease in rats. Periodontitis was induced in thirty six Wistar rats: a first mandibular molar was randomly assigned to receive a ligature, whereas the contralateral molar was left unligated. Animals were randomly assigned to two groups and treated topically, three times a day, for 11 days, as follows: Control Group - vehicle-treated (n = 18), and Test Group - OE 1493-treated (n = 18). The rats were sacrificed on the 12th day. Morphometrical measurements from the cementoenamel junction to the bone crest were performed to determine alveolar bone loss, using standardized photographs. Single- and multi-dose acute toxicity assays were carried out after OE 1493 treatment. Morphometrical analysis demonstrated that topically-administered OE 1493 showed no effect on reducing bone loss when compared with the control group (p > 0.05). In addition, OE 1493 did not present toxicity. Within the limits of this investigation, it may be concluded that OE 1493 did not show any positive influence on the progression of ligature-induced periodontitis in rats, when administered according to the regimen used in the present study.
Asunto(s)
Ipomoea/química , Periodontitis/tratamiento farmacológico , Fitoterapia , Extractos Vegetales/uso terapéutico , Pérdida de Hueso Alveolar/tratamiento farmacológico , Animales , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Masculino , Distribución Aleatoria , Ratas , Ratas Wistar , Factores de TiempoRESUMEN
The aim of this study was to evaluate the effect of an organic extract obtained from Ipomoea alba L. (Convolvulaceae or OE 1493), on experimental periodontal disease in rats. Periodontitis was induced in thirty six Wistar rats: a first mandibular molar was randomly assigned to receive a ligature, whereas the contralateral molar was left unligated. Animals were randomly assigned to two groups and treated topically, three times a day, for 11 days, as follows: Control Group - vehicle-treated (n = 18), and Test Group - OE 1493-treated (n = 18). The rats were sacrificed on the 12th day. Morphometrical measurements from the cementoenamel junction to the bone crest were performed to determine alveolar bone loss, using standardized photographs. Single- and multi-dose acute toxicity assays were carried out after OE 1493 treatment. Morphometrical analysis demonstrated that topically-administered OE 1493 showed no effect on reducing bone loss when compared with the control group (p > 0.05). In addition, OE 1493 did not present toxicity. Within the limits of this investigation, it may be concluded that OE 1493 did not show any positive influence on the progression of ligature-induced periodontitis in rats, when administered according to the regimen used in the present study.