RESUMEN
BACKGROUND: Cassia angustifolia, or senna, is a plant belonging to the Fabaceae family, widely used as a laxative and as a colouring agent in hair dyes. Senna is rarely reported as an occupational allergic sensitizer in the current literature. AIMS: To describe the case and diagnostic approach of a suspected occupational senna allergy. CASE REPORT: A male phytopharmaceutical warehouse worker reported bronchial, conjunctival and nasal symptoms immediately upon exposure to senna. We were able to document in vitro sensitization, finding IgE-binding proteins in senna, and in vivo sensitization through positive skin tests and conjunctival provocation test. CONCLUSION: Our study confirms that senna may cause occupational rhinoconjunctivitis symptoms with an IgE-dependent mechanism and is the first to confirm it through specific conjunctival provocation test.
Asunto(s)
Exposición Profesional/efectos adversos , Extracto de Senna/efectos adversos , Pruebas de Provocación Bronquial/métodos , Conjuntivitis Alérgica/etiología , Humanos , Hipersensibilidad/complicaciones , Inmunoglobulina E/análisis , Inmunoglobulina E/sangre , Masculino , Persona de Mediana Edad , Senna/efectos adversosRESUMEN
No disponible
Asunto(s)
Humanos , Masculino , Lactante , Hipersensibilidad a los Alimentos/diagnóstico , Ajo/efectos adversos , Lectinas de Plantas/efectos adversos , Eritema/etiología , Pruebas Intradérmicas , Factores de RiesgoAsunto(s)
Alérgenos/inmunología , Antígenos de Plantas/inmunología , Proteínas Portadoras/inmunología , Morus/inmunología , Proteínas de Plantas/inmunología , Polen/inmunología , Rinitis Alérgica Estacional/inmunología , Adulto , Electroforesis en Gel de Poliacrilamida , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
No disponible
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Humanos , Masculino , Femenino , Adolescente , Adulto Joven , Adulto , Persona de Mediana Edad , Anciano , Polen/efectos adversos , Polen/inmunología , Rinitis Alérgica Estacional/diagnóstico , Rinitis Alérgica Estacional/inmunología , Proteínas Ligadas a Lípidos/análisis , Profilinas/análisis , Profilinas/inmunología , Morus/efectos adversos , Alérgenos/análisis , Desensibilización Inmunológica/métodos , Inmunoglobulina E/análisisAsunto(s)
Anafilaxia/diagnóstico , Cocos/inmunología , Hipersensibilidad/diagnóstico , Inmunoterapia , Insuficiencia Respiratoria/diagnóstico , Adolescente , Anafilaxia/inmunología , Antígenos de Plantas/inmunología , Reacciones Cruzadas , Conducta Alimentaria , Frutas , Humanos , Hipersensibilidad/inmunología , Hipersensibilidad/terapia , Inmunoglobulina E/sangre , Extractos Vegetales , Insuficiencia Respiratoria/inmunología , Pruebas CutáneasAsunto(s)
Alérgenos/inmunología , Cannabis/inmunología , Proteínas Portadoras/inmunología , Dermatitis Alérgica por Contacto/diagnóstico , Urticaria/diagnóstico , Adulto , Dermatitis Alérgica por Contacto/complicaciones , Humanos , Inmunoglobulina E/sangre , Masculino , Fumar Marihuana , Exposición Profesional/efectos adversos , Extractos Vegetales/inmunología , Estabilidad Proteica , Pruebas Cutáneas , Urticaria/etiologíaAsunto(s)
Humanos , Masculino , Femenino , Profilinas/efectos adversos , Profilinas , Profilinas/inmunología , Alergia e Inmunología/instrumentación , Hipersensibilidad/diagnóstico , Hipersensibilidad/tratamiento farmacológico , Polen/efectos adversos , Polen/inmunología , Western Blotting/métodos , Western Blotting/normas , Western Blotting , Pruebas Cutáneas/instrumentación , Pruebas Cutáneas/métodos , Pruebas CutáneasRESUMEN
AIMS: To investigate the effect of seven wine phenolic compounds and six oenological phenolic extracts on the growth of pathogenic bacteria associated with respiratory diseases (Pseudomonas aeruginosa, Staphylococcus aureus, Moraxella catarrhalis, Enterococcus faecalis, Streptococcus sp Group F, Streptococcus agalactiae and Streptococcus pneumoniae). METHODS AND RESULTS: Antimicrobial activity was determined using a microdilution method and quantified as IC(50) . Mor. catarrhalis was the most susceptible specie to phenolic compounds and extracts. Gallic acid and ethyl gallate were the compounds that showed the greatest antimicrobial activity. Regarding phenolic extracts, GSE (grape seed extract) and GSE-O (oligomeric-rich fraction from GSE) were the ones that displayed the strongest antimicrobial effects. CONCLUSIONS: Results highlight the antimicrobial properties of wine phenolic compounds and oenological extracts against potential respiratory pathogens. The antimicrobial activity of wine phenolic compounds was influenced by the type of phenolic compounds. Gram-negative bacteria were more susceptible than Gram-positive bacteria to the action of phenolic compounds and extracts; however, the effect was species-dependent. SIGNIFICANCE AND IMPACT OF STUDY: The ability to inhibit the growth of respiratory pathogenic bacteria as shown by several wine phenolic compounds and oenological extracts warrants further investigations to explore the use of grape and wine preparations in oral hygiene.
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Antibacterianos/farmacología , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Extracto de Semillas de Uva/farmacología , Fenoles/farmacología , Vino , Ácido Gálico/análogos & derivados , Ácido Gálico/farmacología , Humanos , Concentración 50 Inhibidora , Pruebas de Sensibilidad Microbiana , Boca/microbiologíaAsunto(s)
Anafilaxia/etiología , Desensibilización Inmunológica , Hipersensibilidad a los Alimentos , Frutas , Olea , Polen/inmunología , Adolescente , Alérgenos/inmunología , Anafilaxia/terapia , Antígenos de Plantas/inmunología , Frutas/inmunología , Humanos , Inmunoglobulina E/inmunología , Pruebas Inmunológicas/métodos , Masculino , Olea/inmunología , Pruebas CutáneasRESUMEN
Background: Bee pollen has been proposed as a food supplement, but it can be a dangerous food for people with allergy. We study an allergic reaction after ingestion of bee pollen in a 4-year-old boy who had developed rhinitis in the last spring and autumn. Methods: We performed a prick-by-prick test with bee pollen and skin prick tests with the most important local pollens, house dust mites, common fungi, and animal danders. The levels of serum tryptase, serum total IgE and specific IgE against bee venom and local pollen extracts were determined. The composition of the bee pollen was analysed and SDS-PAGE immunoblotting and blotting-inhibition were carried out. Results: Prick tests were positive to bee pollen and all local pollens extracts and negative to any other allergen sources. The bee pollen sample contained pollens from Quercus genus, and Asteraceae (Compositae) and Rosaceae families. Total IgE was 435kU/l. Serum specific IgE to bee pollen was 6kU/l and greater than 0.35kU/L against pollens from Artemisia vulgaris, Taraxacum officinalis, Cupressus arizonica, Olea europaea, Platanus acerifolia and Lolium perenne as well as to n Art v 1 and other pollen marker allergens. Tryptase level was 3.5mcg/mL. SDS-PAGE immunoblotting-inhibition points to Asteraceae pollen as the possible cause of the allergic reaction. Conclusion: Foods derived from bees can be dangerous to people with allergy to pollen
No disponible
Asunto(s)
Humanos , Polen/efectos adversos , Rinitis Alérgica Estacional/complicaciones , Suplementos Dietéticos/efectos adversos , Hipersensibilidad a los Alimentos/complicaciones , Miel/efectos adversosRESUMEN
Background: Allergen cross-reactivity between tobacco and other species of Solanaceae family (tomato, potato, aubergine and eggplant) have been reported. We have recently studied IgE response to tobacco in asthmatic patients sensitised to Lolium perenne (Perennial rye grass pollen) and have found that 30% of the tobacco responsive patients also have latex sensitisation. Objective: The aim of our study was to investigate the possibility of cross-reactivity between tobacco and latex in asthmatic patients with IgE response to latex. Methods: A study was performed on tobacco and latex exposure in 15 patients who suffered from asthma and latex sensitisation and who were randomly chosen from our database of latex-sensitive patients. To identify tobacco and latex as possible allergens that might cause clinical specific responses, all these patients were tested with prick-tests, specific IgE to tobacco, latex and related allergens, bronchial challenge, and patch tests with tobacco, latex and nicotine. Immunological response was evaluated with immunoblotting, immunoblotting-inhibition and EAST-inhibition tests. Results: Positive prick and bronchial challenge with specific IgE>0.35kU/L to tobacco was demonstrated in 11 asthmatics who were also sensitised to rye grass. Tobacco IgE level was related with sensitisation to latex (p<0.002), but not to other vegetables belonging to the Solanaceae family. EAST-inhibition and immunoblotting-inhibition showed the existence of cross-reactivity between tobacco and latex. Conclusions: Cross-reactivity exists between latex and tobacco allergens. Smoker patients with IgE response to tobacco may be a risk population for latex sensitisation
No disponible
Asunto(s)
Humanos , Masculino , Femenino , Asma/inducido químicamente , Asma/complicaciones , Nicotiana/efectos adversos , Látex/efectos adversos , Reactividad-Estabilidad , Polen/efectos adversos , Pruebas del Parche/métodos , Pruebas del Parche , Hipersensibilidad Inmediata/diagnóstico , Inmunoglobulina E/análisis , Alérgenos/efectos adversos , Alérgenos , Alérgenos/aislamiento & purificación , 28599 , Hipersensibilidad al Látex/inducido químicamente , Hipersensibilidad al Látex/complicaciones , Hipersensibilidad al Látex/diagnósticoRESUMEN
No disponible
Asunto(s)
Humanos , Masculino , Niño , Cynara/efectos adversos , Hipersensibilidad/complicaciones , Hipersensibilidad/diagnóstico , Conjuntivitis/complicaciones , Conjuntivitis/diagnóstico , Conjuntivitis Alérgica/complicaciones , Conjuntivitis Alérgica/diagnóstico , Conjuntivitis Alérgica/terapia , Western Blotting , Polen/efectos adversos , Rinitis Alérgica EstacionalRESUMEN
BACKGROUND: Bee pollen has been proposed as a food supplement, but it can be a dangerous food for people with allergy. We study an allergic reaction after ingestion of bee pollen in a 4-year-old boy who had developed rhinitis in the last spring and autumn. METHODS: We performed a prick-by-prick test with bee pollen and skin prick tests with the most important local pollens, house dust mites, common fungi, and animal danders. The levels of serum tryptase, serum total IgE and specific IgE against bee venom and local pollen extracts were determined. The composition of the bee pollen was analysed and SDS-PAGE immunoblotting and blotting-inhibition were carried out. RESULTS: Prick tests were positive to bee pollen and all local pollens extracts and negative to any other allergen sources. The bee pollen sample contained pollens from Quercus genus, and Asteraceae (Compositae) and Rosaceae families. Total IgE was 435 kU/l. Serum specific IgE to bee pollen was 6 kU/l and greater than 0.35 kU/L against pollens from Artemisia vulgaris, Taraxacum officinalis, Cupressus arizonica, Olea europaea, Platanus acerifolia and Lolium perenne as well as to n Art v 1 and other pollen marker allergens. Tryptase level was 3.5 mcg/mL. SDS-PAGE immunoblotting-inhibition points to Asteraceae pollen as the possible cause of the allergic reaction. CONCLUSION: Foods derived from bees can be dangerous to people with allergy to pollen.
Asunto(s)
Alérgenos/inmunología , Venenos de Abeja/inmunología , Suplementos Dietéticos/efectos adversos , Hipersensibilidad/diagnóstico , Polen/inmunología , Animales , Artemisia/inmunología , Venenos de Abeja/efectos adversos , Abejas/inmunología , Preescolar , Humanos , Hipersensibilidad/inmunología , Hipersensibilidad/fisiopatología , Inmunoglobulina E/sangre , Masculino , Polen/efectos adversos , Rinitis , Estaciones del Año , Pruebas CutáneasAsunto(s)
Asma/diagnóstico , Hipersensibilidad a los Alimentos/diagnóstico , Rinitis Alérgica Estacional/diagnóstico , Antígenos de Plantas/inmunología , Asma/sangre , Asma/etiología , Asma/fisiopatología , Western Blotting , Niño , Reacciones Cruzadas , Cynara/inmunología , Hipersensibilidad a los Alimentos/sangre , Hipersensibilidad a los Alimentos/etiología , Hipersensibilidad a los Alimentos/fisiopatología , Calor , Humanos , Inmunización , Inmunoglobulina E/sangre , Masculino , Polen/efectos adversos , Polen/inmunología , Prurito , Rinitis Alérgica Estacional/sangre , Rinitis Alérgica Estacional/complicaciones , Rinitis Alérgica Estacional/fisiopatología , Estaciones del Año , Pruebas Cutáneas , VerdurasAsunto(s)
Anafilaxia/etiología , Artemisia/inmunología , Hipersensibilidad a los Alimentos/etiología , Mangifera/inmunología , Adulto , Anafilaxia/inmunología , Reacciones Cruzadas , Femenino , Hipersensibilidad a los Alimentos/inmunología , Humanos , Immunoblotting , Polen/inmunología , Pruebas CutáneasRESUMEN
BACKGROUND: The seeds of Plantago ovata (psyllium, ispaghula) used in the manufacture of bulk laxatives are known to be the cause of occupational allergy (rhinitis, asthma) in health care and pharmaceutical workers. OBJECTIVE: We studied the prevalence of P ovata seed allergy among health care workers in geriatric care homes and compared it with a group of health care professionals not exposed to P ovata seed. Cross reactivity with Plantago lanceolata pollen was also studied. METHODS: Two groups of health professionals were recruited: 58 health care workers from geriatric care homes who were exposed daily to laxatives containing P ovata and 63 nonexposed health care professionals. The prevalence of allergy and sensitization to P ovata seed was determined based on clinical history, skin prick test, and analysis of specific immunoglobulin (Ig) E. IgE immunoblotting was performed to calculate the molecular weights of the P ovata seed allergens. Cross reactivity to P lanceolata pollen was studied by enzyme allergosorbent test (EAST) and immunoblot inhibition techniques. RESULTS: The prevalence of sensitization and clinical allergy to P ovata seed in the exposed group was 13.8% and 8.6%, respectively. No sensitization was observed in the nonexposed group. IgE-binding proteins of 17, 20, 25, 32-34, 54, 73-77, and > 97 kDa were identified. EAST inhibition and immunoblot inhibition demonstrated the existence of cross reactivity between P ovata seed and P lanceolata pollen extracts. CONCLUSIONS: The rate of sensitization to P ovata seed is high among health care workers in geriatric care homes (13.8%). A mild cross reactivity between P ovata seed and P lanceolata pollen was observed.