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1.
Expert Rev Vaccines ; 16(2): 137-149, 2017 02.
Artículo en Inglés | MEDLINE | ID: mdl-27599605

RESUMEN

INTRODUCTION: Respiratory infections have an enormous, worldwide epidemiologic impact on humans and animals. Among the prophylactic measures, vaccination has the potential to neutralize this impact. New technologies for vaccine production and delivery are of importance in this field since they offer the potential to develop new immunization approaches overriding the current limitations that comprise high cost, safety issues, and limited efficacy. Areas covered: In the present review, the state of the art in developing plant-based vaccines against respiratory diseases is presented. The review was based on the analysis of current biomedical literature. Expert commentary: Preclinical and clinical evaluations of several vaccine candidates against influenza, tuberculosis, respiratory syncytial virus, pneumonia, anthrax and asthma are discussed and placed in perspective.


Asunto(s)
Vacunas Bacterianas/inmunología , Plantas Modificadas Genéticamente/metabolismo , Infecciones del Sistema Respiratorio/prevención & control , Vacunas Virales/inmunología , Animales , Vacunas Bacterianas/aislamiento & purificación , Ensayos Clínicos como Asunto , Modelos Animales de Enfermedad , Descubrimiento de Drogas/tendencias , Evaluación Preclínica de Medicamentos , Humanos , Plantas Modificadas Genéticamente/genética , Vacunas Sintéticas/inmunología , Vacunas Sintéticas/aislamiento & purificación , Vacunas Virales/aislamiento & purificación
2.
Avian Dis ; 60(4): 784-791, 2016 12.
Artículo en Inglés | MEDLINE | ID: mdl-27902910

RESUMEN

Globally, avian influenza (AI) is a serious problem in poultry farming. Despite vaccination, the prevalence of AI in México highlights the need for new approaches to control AI and to reduce the economic losses associated with its occurrence in susceptible birds. Recombinant proteins from avian influenza virus (AIV) have been expressed in different organisms, such as plants. The present study investigated the feasibility of designing and expressing the HA protein of AIV in the transplastomic microalga Chlamydomonas reinhardtii as a novel approach for AIV control and taking advantage of culture conditions, its reproductive range, and safe use in consideration of the generally regarded as safe food ingredient regulatory classification. The results showed that the HA protein of AIV in C. reinhardtii presents antigenic activity by western blot test and through its application in chickens, demonstrating its feasibility as a recombinant antigen against AIV.


Asunto(s)
Chlamydomonas reinhardtii/genética , Glicoproteínas Hemaglutininas del Virus de la Influenza/inmunología , Subtipo H5N2 del Virus de la Influenza A/inmunología , Vacunas contra la Influenza/inmunología , Gripe Aviar/inmunología , Enfermedades de las Aves de Corral/inmunología , Animales , Anticuerpos Antivirales/inmunología , Pollos , Chlamydomonas reinhardtii/metabolismo , Cloroplastos/genética , Cloroplastos/metabolismo , Evaluación Preclínica de Medicamentos , Expresión Génica , Glicoproteínas Hemaglutininas del Virus de la Influenza/administración & dosificación , Glicoproteínas Hemaglutininas del Virus de la Influenza/genética , Subtipo H5N2 del Virus de la Influenza A/genética , Vacunas contra la Influenza/administración & dosificación , Vacunas contra la Influenza/genética , Gripe Aviar/prevención & control , Gripe Aviar/virología , Enfermedades de las Aves de Corral/prevención & control , Enfermedades de las Aves de Corral/virología
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