Blood orange (Citrus sinensis) as a rich source of nutraceuticals: investigation of bioactive compounds in different parts of the fruit by HPLC-PDA/MS.

This study is part of a wider investigation aimed to sustain the economical value of the by-products generated by the Citrus industry. In particular, the protected geographic indication (PGI) red orange of sicily (known as blood orange) has been analysed by HPLC and by the enzymatic AOAC method. All the by-products contain significant amounts of biologically active compounds (limonoids and flavonoids). The decanted pulps were the most abundant, with the highest amount of flavonoids (130 g/kg) and high amount of limonoids (5.5 g/kg). Seeds were the best source of limonoids with about 10 g/kg. Low amount of anthocyanins were found only in coarse pulps and waste water. The total, the insoluble and the soluble dietary fibre (TDF, IDF and SDF respectively) were also determined. The pulps resulted to be the best source of dietary fibre, based on the amount and on the best insoluble/soluble ratio.

Distribution of bioactives in entire mill chain from the drupe to the oil and wastes.

Industrial olive oil production generates large amounts of by-products, mainly pomaces and wastewaters. The latter, in particular, represents serious environmental problems requiring special treatments prior to disposal. While olive pomace finds use as alternative energy source, wastewaters still remain a task since it is not reusable, representing an additional cost on olive oil for its treatments. This study is a "comprehensive" overview on the distribution of bioactives in entire mill chain from the drupe to the oil and wastes. Identification was achieved through liquid chromatography coupled with mass spectrometry, spectrofotometric and fluorimetric detection. Phenols resulted the most abundant class of substances, with the highest hydroxytyrosol amounts in wastewater (214 mg/kg). Pomace contained a total of 304 mg/kg in terms of bioactives, thus representing a potential food supplement ingredient for functional foods with high nutritional values.