Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Más filtros

Bases de datos
Tipo del documento
País de afiliación
Intervalo de año de publicación
1.
Nucleic Acids Res ; 26(2): 566-75, 1998 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-9421517

RESUMEN

In an attempt to improve physico-chemical and biological properties of peptide nucleic acids (PNAs), particularly water solubility and cellular uptake, the synthesis of chimeric oligomers consisted of PNA and phosphono-PNA analogues (pPNAs) bearing the four natural nucleobases has been accomplished. To produce these chimeras, pPNA monomers of two types containing N-(2-hydroxyethyl)phosphonoglycine, or N-(2-aminoethyl)phosphonoglycine backbone, were used in conjunction with PNA monomers representing derivatives of N-(2-aminoethyl)glycine, or N-(2-hydroxyethyl)glycine. The oligomers obtained were composed of either PNA and pPNA stretches or alternating PNA and pPNA monomers. The examination of hybridization properties of PNA-pPNA chimeras to DNA and RNA complementary strands in comparison with pure PNAs, and pPNAs as well as DNA-pPNA hybrids and DNA fragments confirmed that these chimeras form stable complexes with complementary DNA and RNA fragments. They were found to be resistant to degradation by nucleases. All these properties together with good solubility in water make PNA-pPNA hybrids promising for further evaluation as potential therapeutic agents.


Asunto(s)
Ácidos Nucleicos/síntesis química , Oligonucleótidos/síntesis química , Péptidos/síntesis química , Fenómenos Químicos , Química Física , ADN Complementario/química , Dimerización , Estabilidad de Medicamentos , Glicina/análogos & derivados , Glicina/química , Calor , Hibridación de Ácido Nucleico , Ácidos Nucleicos/química , Ácidos Nucleicos/metabolismo , Nylons/química , Organofosfonatos/química , Péptidos/química , Péptidos/metabolismo , Fosfodiesterasa I , Hidrolasas Diéster Fosfóricas/metabolismo , ARN Complementario/química , Endonucleasas Específicas del ADN y ARN con un Solo Filamento/metabolismo , Solubilidad , Rayos Ultravioleta
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA