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1.
J Nutr Biochem ; 99: 108856, 2022 01.
Artículo en Inglés | MEDLINE | ID: mdl-34517098

RESUMEN

High-fat, high-sugar diet (HFHS) induced leptin resistance and intestinal epithelial dysfunction is implicated in hyperphagia and metabolic disorders. Numerous studies have demonstrated the efficacy of dietary interventions for reducing appetite. This study aims to investigate whether triacylglycerol rich in DHA (DHA-TG) could regulate appetite in mice fed with a HFHS diet and the mechanism by which it achieves that. DHA-TG could reduce food intake and regulate neuropeptides (POMC, AgRP, and NPY) expression in HFHS diet-fed mice. Hypothalamic transcriptome analysis reveals that these effects might be attributed to the role of DHA-TG in modulating hormone secretion and digestive system process. According to ELISA and RT-qPCR analysis, DHA-TG ameliorated leptin secretion and attenuated central leptin resistance induced by HFHS diet feeding. Besides, DHA-TG prevented the damage of intestinal epithelial barrier in nutritive obese mice by improving leptin sensitivity. Based on jejunal transcriptome analysis, DHA-TG also protected intestinal endocrine function, especially the secretion of another anorectic hormone, cholecystokinin (CCK), in HFHS diet-fed mice. Furthermore, DHA-TG was ineffective in repressing appetite, and improving gut leakage in leptin-deficient mice (ob/ob mice). In conclusion, DHA-TG has a potential to regulate appetite with the action of leptin, and intestinal epithelial functions in HFHS diet-fed mice.


Asunto(s)
Apetito , Dieta de Carga de Carbohidratos , Dieta Alta en Grasa , Ácidos Docosahexaenoicos/metabolismo , Intestinos/metabolismo , Leptina/metabolismo , Triglicéridos/metabolismo , Animales , Carbohidratos de la Dieta/análisis , Carbohidratos de la Dieta/metabolismo , Grasas de la Dieta/análisis , Grasas de la Dieta/metabolismo , Ácidos Docosahexaenoicos/análisis , Ingestión de Alimentos , Células Epiteliales/metabolismo , Humanos , Hipotálamo/metabolismo , Intestinos/citología , Masculino , Ratones , Ratones Endogámicos C57BL , Neuropéptidos/genética , Neuropéptidos/metabolismo , Triglicéridos/análisis
2.
J Sci Food Agric ; 102(5): 1978-1986, 2022 Mar 30.
Artículo en Inglés | MEDLINE | ID: mdl-34519034

RESUMEN

BACKGROUND: Oyster's lipid degradation leads to a decrease in edible and nutritional value. Curcumin-mediated photodynamic treatment (PDT) is an innovative non-thermal technology, although evaluation of the oyster's lipid degradation has been scarce. In the present study, we investigated peroxide value, thiobarbituric acid reactive substance, triacylglycerol and free fatty acids to evaluate the effect of curcumin-mediated PDT on lipid degradation of oysters during refrigerated storage. RESULTS: The results showed that curcumin-mediated PDT could delay oyster's lipid degradation. Next, the activities of enzymes were detected to determine the mechanisms behind the effects of curcumin-mediated PDT. It was revealed that the activities of lipase, phospholipase A2 (PLA2 ), phospholipase C (PLC), phospholipase D (PLD) and lipoxygenase (LOX) were significantly inhibited after curcumin-mediated PDT (P < 0.05). Furthermore, 16 s rRNA analysis established that the relative abundances of Pseudoalteromonas and Psychrilyobacter were reduced by 51.58% and 43.82%, respectively, after curcumin-mediated PDT. CONCLUSION: Curcumin-mediated PDT could delay oyster's lipid degradation by inhibiting the activities of lipase, PLA2 , PLC, PLD and LOX, as well as by changing the oyster's microbial composition, reducing the relative abundance of Pseudoalteromonas and Psychrilyobacter. © 2021 Society of Chemical Industry.


Asunto(s)
Curcumina , Conservación de Alimentos , Lípidos , Ostreidae , Fármacos Fotosensibilizantes , Animales , Curcumina/química , Conservación de Alimentos/métodos , Lípidos/química , Ostreidae/química , Ostreidae/microbiología , Ostreidae/efectos de la radiación , Fosfolipasas A2/análisis , Fármacos Fotosensibilizantes/química , Refrigeración
3.
Food Funct ; 12(10): 4644-4653, 2021 May 21.
Artículo en Inglés | MEDLINE | ID: mdl-33912875

RESUMEN

Chronic diseases, such as obesity, cause great harm to human health. Conventional drugs have promising therapeutic effects but also cause significant side effects. Functional foods are an excellent therapeutic alternative to pharmaceuticals, as they have fewer side effects. However, screening for active ingredients in natural foods is difficult. In this study, a novel pancreatic lipase inhibitor screening strategy, guided by the drug molecule orlistat, was combined with experimental verification. Twenty compounds from natural foods were evaluated based on the characteristics of orlistat interaction with pancreatic lipase. The characteristics of 13 molecules were comparable to those of orlistat. The pancreatic lipase inhibition rates of curcumin and sinensetin were 82.42 ± 0.50% and 81.07 ± 2.05%, respectively, and their IC50 values were 0.971 mM and 0.526 mM, respectively; both the inhibition rates as well as IC50 values were similar to those of orlistat. Curcumin and sinensetin prevented weight gain in mice by 69.17% and 52.29%, respectively, compared to orlistat. Curcumin and sinensetin did not cause significant organ damage in vivo, but significantly reduced the contents of triglycerides and cholesterol in blood and lipids in the liver, protecting liver function. Furthermore, 57 328 molecules in the Chinese Natural Product Database library were screened, and 20 potentially active molecules, found to be highly efficient in our study, were selected. Thus, we successfully established an efficient and accurate strategy for screening active ingredients in natural foods under the guidance of a drug molecule, providing valuable insights for functional food development.


Asunto(s)
Inhibidores Enzimáticos/farmacología , Alimentos Funcionales , Lipasa/efectos de los fármacos , Páncreas/efectos de los fármacos , Animales , Fármacos Antiobesidad/farmacología , Colesterol/sangre , Evaluación Preclínica de Medicamentos , Flavonoides , Lípidos/sangre , Hígado/metabolismo , Hígado/patología , Masculino , Ratones , Ratones Endogámicos C57BL , Simulación de Dinámica Molecular , Obesidad/tratamiento farmacológico , Orlistat/uso terapéutico , Triglicéridos/sangre , Aumento de Peso
4.
Food Funct ; 12(10): 4654-4669, 2021 May 21.
Artículo en Inglés | MEDLINE | ID: mdl-33913445

RESUMEN

Sargassum fusiforme, a nutritious edible brown alga, has been widely suggested to play an important role in the development of functional food because of its multiple biological activities. The aim of this study was to explore the anti-obesity effect of the combination of Sargassum fusiforme with extracts of fruit and vegetable by comparing the effects of Sargassum fusiforme (S), Sargassum fusiforme together with pomegranate peel extract (SP), Sargassum fusiforme together with turmeric extract (ST) and Sargassum fusiforme together with turmeric extract and pomegranate peel extract (C) on diet-induced obese C57BL/6J mice. Long-term consumption of a high-fat diet can lead to high levels of blood lipid, increase adipocyte size, and cause lipid metabolism dysfunction and gut microbiota dysbiosis. According to the results of the experiments, SP and ST were more effective in reducing lipid levels and fat accumulation than S; and, C exhibited the strongest efficacy compared with the other three supplements. ST and C also regulated adipocytokines and had significant effects on the gene expression of lipid metabolism. We also found that C alleviated the imbalance of intestinal flora caused by a high-fat diet to a certain extent. In conclusion, SP, ST and C have anti-obesity potentials, which can be used as alternative ingredients in the formula of functional food for obese people.


Asunto(s)
Obesidad/tratamiento farmacológico , Extractos Vegetales/farmacología , Granada (Fruta)/química , Sargassum/química , Tejido Adiposo/metabolismo , Animales , Curcuma , Dieta Alta en Grasa/efectos adversos , Suplementos Dietéticos , Inhibidores Enzimáticos/farmacología , Frutas/química , Microbioma Gastrointestinal/efectos de los fármacos , Glucosa , Metabolismo de los Lípidos , Hígado/patología , Masculino , Ratones , Ratones Endogámicos C57BL , Páncreas/patología
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