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Nat Prod Res ; 35(10): 1690-1695, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-31198054

RESUMEN

This work aimed to obtain and characterize protease inhibitors from A. colubrina leaves and evaluate their potential as inflammatory mediator and cell viability. The protein extract was analyzed and characterized by SDS-PAGE, RP-HPLC-PDA, MALDI-TOF/MS and Zeta potential. Bioassays were conducted in order to evaluate the cell viability in RAW 264.7, in vitro (NO and TNF-α production inhibition) and in vivo anti-inflammatory potential, inhibition rate of trypsin and hemagglutination activity from protein extract. The results revealed the presence of bands at 14, 21 and 30 kDa in SDS-PAGE, the RP-HPLC-PDA analysis showed peaks at 12, 13, 28 and 40 minutes and MALDI-TOF/MS showed peaks with 3.4, 4.7, 5.6, 9.4 and 11.2 kDa. The protein extracts presented enzymatic activity inhibition of trypsin (IC50 59.2 µgmL-1), did not show any cytotoxicity to RAW264.7 cells, hemagglutination 8HU and insignificant reduction in NO and TNF-α production and reduced anti-inflammatory potential in vivo compared to dexamethasone.


Asunto(s)
Antiinflamatorios/farmacología , Fabaceae/química , Inhibidores de Proteasas/farmacología , Animales , Linaje de la Célula/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Cromatografía Líquida de Alta Presión , Macrófagos/efectos de los fármacos , Macrófagos/patología , Ratones , Tamaño de la Partícula , Extractos Vegetales/farmacología , Hojas de la Planta/química , Células RAW 264.7 , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Electricidad Estática , Tripsina/metabolismo
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