RESUMEN
In the present study, ZnO-Fe3O4 nanoparticles were synthesized using the leaves of Camellia sinensis and immobilized in crosslinked alginate-chitosan polymer beads and tested for their photocatalytic applications. The prepared nanocomposite was used for the simultaneous adsorption and photocatalytic degradation of acid violet 7 (AV7) dye. The optimization of reaction conditions ensured higher dye removal efficacy up to 94.21 ± 1.02% using the nanocomposite under UV-C irradiation of 365 nm. The kinetics of the adsorption study fitted well with the pseudo-first-order reaction. The Langmuir model fitted better to the adsorption isotherms compared to the Freundlich and Temkin models. The mechanism of degradation was studied by analyzing the treated AV7 solution. The removal efficiency in tap water, groundwater, and lake water was 83.23 ± 0.4%, 69.13 ± 1.6%, and 67.89 ± 0.3%, respectively. The residual toxicity of the degraded AV7 solution was tested on model organisms like freshwater algae, Scenedesmus sp., and plant model, Allium cepa, demonstrating the lower toxicity of the degraded AV7 product. Finally, a cost-benefit analysis of the experiments was also carried out.
Asunto(s)
Camellia sinensis , Quitosano , Nanocompuestos , Contaminantes Químicos del Agua , Óxido de Zinc , Adsorción , Alginatos , Compuestos Azo , Concentración de Iones de Hidrógeno , Cinética , Naftalenosulfonatos , Extractos Vegetales , AguaRESUMEN
Antimicrobial resistance is a major health concern worldwide. A narrowing of the antibiotic development pipeline and a resurgence in public opinion towards 'natural' therapies have renewed the interest in using essential oils as antimicrobial agents. The drawbacks of bulk dosing of essential oils can be mitigated by formulating them as micro- and nanoemulsions. These emulsions have an added advantage as they are in the nanometre size range whose thermodynamic properties enable them to be used as an effective drug delivery system. This review describes the current work on the antimicrobial activities of essential oil micro- and nanoemulsions and their role as drug delivery vehicles.
Asunto(s)
Antibacterianos/farmacología , Enfermedades Transmisibles/tratamiento farmacológico , Nanoestructuras , Aceites Volátiles/farmacología , Vehículos Farmacéuticos , Aceites de Plantas/farmacología , Antibacterianos/administración & dosificación , Antibacterianos/uso terapéutico , Antifúngicos/farmacología , Antifúngicos/uso terapéutico , Farmacorresistencia Bacteriana , Emulsiones , Enterobacteriaceae/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Humanos , Pruebas de Sensibilidad Microbiana , Aceites Volátiles/administración & dosificación , Aceites Volátiles/uso terapéutico , Aceites de Plantas/uso terapéuticoRESUMEN
The current study was aimed to explore the differential effects on Gram-positive and Gram-negative freshwater sediment bacterial isolates upon exposure to nano-particles and bulk particles of Al2O3 at low concentrations (0.25, 0.5, and 1 mg/L). The Gram-negative Pseudomonas aeruginosa was more susceptible to both the nano-forms and bulk forms than the Gram-positive Bacillus altitudinis. The generation of reactive oxygen species (ROS) and release of lipopolysaccharide due to membrane damage were dependent on the dose of nano-Al2O3. The Fourier transform infrared spectroscopy (FT-IR) studies confirmed the attachment of nano-Al2O3 on bacterial cells, which may lead to subsequent changes in the cell membrane composition and integrity. Internalization of nano-Al2O3 was estimated to be more for P. aeruginosa than for B. altitudinis cells. As a role of defense mechanism, the biofilm formation and production of extracellular polymeric substances (EPSs; polysaccharide and protein) were increased with respect to the concentration of toxicant. Nano-Al2O3 was estimated to cause more DNA damage than the bulk particles in both Gram-positive and Gram-negative bacterial strains.
Asunto(s)
Óxido de Aluminio/toxicidad , Bacillus/efectos de los fármacos , Nanopartículas/toxicidad , Pseudomonas aeruginosa/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidad , Óxido de Aluminio/química , Bacillus/genética , Bacillus/aislamiento & purificación , Bacillus/metabolismo , Biopolímeros/metabolismo , Membrana Celular/química , Membrana Celular/efectos de los fármacos , Daño del ADN , Agua Dulce/microbiología , Sedimentos Geológicos/microbiología , Nanopartículas/química , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/aislamiento & purificación , Pseudomonas aeruginosa/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Espectroscopía Infrarroja por Transformada de Fourier , Contaminantes Químicos del Agua/químicaRESUMEN
Despite the extensive use of nanoparticles (NPs) in various fields, adequate knowledge of human health risk and potential toxicity is still lacking. The human lymphocytes play a major role in the immune system, and it can alter the antioxidant level when exposed to NPs. Identification of the hazardous NPs was done using in vitro toxicity tests and this study mainly focuses on the comparative in vitro cytotoxicity and genotoxicity of four different NPs including cobalt (II, III) oxide (Co3O4), iron (III) oxide (Fe2O3), silicon dioxide (SiO2), and aluminum oxide (Al2O3) on human lymphocytes. The Co3O4 NPs showed decrease in cellular viability and increase in cell membrane damage followed by Fe2O3, SiO2, and Al2O3 NPs in a dose-dependent manner after 24 h of exposure to human lymphocytes. The oxidative stress was evidenced in human lymphocytes by the induction of reactive oxygen species, lipid peroxidation, and depletion of catalase, reduced glutathione, and superoxide dismutase. The Al2O3 NPs showed the least DNA damage when compared with all the other NPs. Chromosomal aberration was observed at 100 µg/ml when exposed to Co3O4 NPs and Fe2O3 NPs. The alteration in the level of antioxidant caused DNA damage and chromosomal aberration in human lymphocytes.
Asunto(s)
Óxido de Aluminio/toxicidad , Cobalto/toxicidad , Compuestos Férricos/toxicidad , Linfocitos/efectos de los fármacos , Nanopartículas/toxicidad , Óxidos/toxicidad , Dióxido de Silicio/toxicidad , Adulto , Antioxidantes/metabolismo , Membrana Celular/patología , Aberraciones Cromosómicas/inducido químicamente , Daño del ADN , Relación Dosis-Respuesta a Droga , Humanos , Técnicas In Vitro , Peroxidación de Lípido/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo , Adulto JovenRESUMEN
Crystalline structure of nanoparticles may influence their physicochemical behaviour as well as their toxicological impact on biota. The differences in orientation of the atoms result in the variations in chemical stability. Thus, toxicological impacts of different crystalline phases of aluminium oxide nanoparticles are expected to vary. The present study brings out a comparative toxicity analysis of γ-phase and α-phase aluminium oxide nanoparticles of comparable hydrodynamic size range towards a freshwater bacterial isolate Bacillus licheniformis at low exposure concentrations (5, 1, 0.5 and 0.05 µg/mL). Upon 2-h exposure, the α-aluminium oxide particles showed lower toxicity than the γ-phase aluminium oxide. The lower level of oxidative stress generation and cell membrane damage in case of the α-phase aluminium oxide nanoparticles substantiated the toxicity results. The involvement of protein, lipopolysaccharides in nanoparticle-cell surface interaction, was noted in both the cases. To conclude, the crystallinity of aluminium oxide nanoparticles played an important role in the interaction and the toxicity response.
Asunto(s)
Óxido de Aluminio/toxicidad , Bacillus/efectos de los fármacos , Nanopartículas del Metal/toxicidad , Contaminantes Químicos del Agua/toxicidad , Membrana Celular/metabolismo , Relación Dosis-Respuesta a Droga , Agua Dulce , Iones , L-Lactato Deshidrogenasa/metabolismo , Luz , Nanotecnología , Estrés Oxidativo , Tamaño de la Partícula , Especies Reactivas de Oxígeno/metabolismo , Dispersión de Radiación , Espectroscopía Infrarroja por Transformada de Fourier , Pruebas de Toxicidad , Difracción de Rayos XRESUMEN
The reactivity and toxicity of the soluble toxicants in the presence of the engineered nanomaterials is not well explored. In this study, the probable effects of TiO2 and Al2O3 nanoparticles (n-TiO2, n-Al2O3) on the toxicity of Cr(VI) were assessed with the dominant freshwater algae, Scenedesmus obliquus, in a low range of exposure concentrations (0.05, 0.5 and 1µg/mL). In the presence of 0.05µg/mL n-TiO2, the toxicity of Cr(VI) decreased considerably, which was presumably due to the Cr(VI) adsorption on the nanoparticle surface leading to its aggregation and precipitation. The elevated n-TiO2 concentrations (0.5 and 1µg/mL) did not significantly influence Cr(VI) bio-availability, and a dose dependent toxicity of Cr(VI) was observed. On the other hand, n-Al2O3 did not have any significant effect on the Cr(VI) toxicity. The microscopic observations presented additional information on the morphological changes of the algal cells in the presence of the binary toxicants. The generation of reactive oxygen species (ROS) suggested contribution of oxidative stress on toxicity and LDH release confirmed membrane permeability of algal cells upon stress.
Asunto(s)
Óxido de Aluminio/toxicidad , Cromo/toxicidad , Nanopartículas del Metal/toxicidad , Scenedesmus/efectos de los fármacos , Titanio/toxicidad , Contaminantes Químicos del Agua/toxicidad , Interacciones Farmacológicas , Agua Dulce , Estrés Oxidativo/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismoRESUMEN
Candida albicans is a common fungal pathogen that causes systemic and superficial infections in most immunocompromised patients. Fluconazole, a synthetic triazole antifungal agent, is the most prescribed drug used in treating this pathogen. But because of its poor solubilization in water and the emergence of resistant strains against this antimycotic drug, we aimed at devising a unique microemulsion drug delivery system for fluconazole against candidiasis. A clear oil-in-water microemulsion system, consisting of clove oil as oil phase, Tween 20 as surfactant, and water as aqueous phase was developed using a ternary phase diagram. Physicochemical characterization was done to understand the internal physicochemical state. The bulk drug, fluconazole, that measured several microns in length was reduced to a 10-65 nm range with no means of high-energy methods as confirmed by transmission electron microscopy. The very small and uniform spherical structure of the drug-loaded microemulsion system could be of high impact to the biological system as the efficacy of fluconazole is greatly improved when compared with its conventional bulk form. The optimized microemulsion exhibited significantly higher antifungal activity at a minimum concentration (8 µg/ml) of fluconazole as examined by fluorescence and scanning electron microscopy. Thus, our report discloses an excellent oral drug delivery system.
Asunto(s)
Antifúngicos/química , Antifúngicos/farmacología , Candida albicans/efectos de los fármacos , Sistemas de Liberación de Medicamentos/métodos , Fluconazol/química , Fluconazol/farmacología , Naranja de Acridina/metabolismo , Administración Oral , Candida albicans/citología , Candida albicans/metabolismo , Candida albicans/ultraestructura , Candidiasis/tratamiento farmacológico , Candidiasis/microbiología , Membrana Celular/efectos de los fármacos , Membrana Celular/metabolismo , Supervivencia Celular/efectos de los fármacos , Química Farmacéutica , Aceite de Clavo/química , Emulsiones , Etidio/metabolismo , Concentración de Iones de Hidrógeno , Interacciones Hidrofóbicas e Hidrofílicas , Solubilidad , Tensoactivos/química , Termodinámica , Viscosidad , Agua/químicaRESUMEN
The growing commercial applications had brought aluminium oxide nanoparticles under toxicologists' purview. In the present study, the cytotoxicity of two different sized aluminium oxide nanoparticles (ANP(1), mean hydrodynamic diameter 82.6±22nm and ANP(2), mean hydrodynamic diameter 246.9±39nm) towards freshwater algal isolate Chlorella ellipsoids at low exposure levels (≤1µg/mL) using sterile lake water as the test medium was assessed. The dissolution of alumina nanoparticles and consequent contribution towards toxicity remained largely unexplored owing to its presumed insoluble nature. Herein, the leached Al(3+) ion mediated toxicity has been studied along with direct particulate toxicity to bring out the dynamics of toxicity through colloidal stability, biochemical, spectroscopic and microscopic analyses. The mean hydrodynamic diameter increased with time both for ANP(1) [82.6±22nm (0h) to 246.3±59nm (24h), to 1204±140nm (72h)] and ANP(2) [246.9±39nm (0h) to 368.28±48nm (24h), to 1225.96±186nm (72h)] signifying decreased relative abundance of submicron sized particles (<1000nm). The detailed cytotoxicity assays showed a significant reduction in the viability dependent on dose and exposure. A significant increase in ROS and LDH levels were noted for both ANPs at 1µg/mL concentration. The zeta potential and FT-IR analyses suggested surface chemical interaction between nanoparticles and algal cells. The substantial morphological changes and cell wall damage were confirmed through microscopic analyses (SEM, TEM, and CLSM). At 72h, significant Al(3+) ion release in the test medium [0.092µg/mL for ANP(1), and 0.19µg/mL for ANP(2)] was noted, and the resulting suspension containing leached ions caused significant cytotoxicity, revealing a substantial ionic contribution. This study indicates that both the nano-size and ionic dissolution play a significant role in the cytotoxicity of ANPs towards freshwater algae, and the exposure period largely determines the prevalent mode of nano-toxicity.
Asunto(s)
Óxido de Aluminio/toxicidad , Chlorella/efectos de los fármacos , Nanopartículas del Metal/toxicidad , Contaminantes Químicos del Agua/toxicidad , Relación Dosis-Respuesta a Droga , Tamaño de la Partícula , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Pesticides are an essential tool in integrated pest management. Nanopermethrin was prepared by solvent evaporation from an oil-in-water volatile microemulsion. The efficacy of the formulated nanopermethrin was tested against Aedes aegypti and the results compared to those of regular, microparticular permethrin. The 24 h LC50 for nanopermethrin and permethrin was found to be 0.0063 and 0.0199 mg/L, respectively. The formulated nanopermethrin was tested for toxicity against non-target organisms. Nanopermethrin did not show antibacterial activity against Escherichia coli (ATCC 13534 and 25922) or against Bacillus subtilis. Phytotoxicity studies of nanopermethrin to the seeds of Lycopersicum esculentum, Cucumis sativus, and Zea mays showed no restraint in root length and germination percentage. In the Allium cepa test, regular microparticular permethrin treatment of 0.13 mg/L showed a mitotic index (MI) of 46.8% and chromosomal aberration of 0.6%, which was statistically significant (p < 0.05) compared to control. No significant differences were observed in 0.13 mg/L nanopermethrin exposure as compared to control (MI of 52.0 and 55.03 % and chromosomal aberration of 0.2 and 0%, respectively). It was concluded that formulated nanopermethrin can be used as a safe and effectual alternative to commercially available permethrin formulation in agricultural practices.
Asunto(s)
Insecticidas/toxicidad , Nanopartículas/toxicidad , Permetrina/toxicidad , Aedes , Animales , Bacillus subtilis/efectos de los fármacos , Cucumis sativus/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Insecticidas/química , Larva , Dosificación Letal Mediana , Solanum lycopersicum/efectos de los fármacos , Microscopía Electrónica de Transmisión , Índice Mitótico , Pruebas de Mutagenicidad , Nanopartículas/química , Cebollas/efectos de los fármacos , Permetrina/química , Raíces de Plantas/efectos de los fármacos , Semillas/efectos de los fármacos , Zea mays/efectos de los fármacosRESUMEN
This study on a microcosm, brings out the temporal changes in physico-chemical behavior of aluminum oxide nanoparticles (for a period of 210 days), at environmentally relevant concentrations (1000 µg/L and below). The dynamics of particle behavior in terms of mean hydrodynamic diameter, specific surface area and dissolution of soluble aluminum and, their possible ecological implications have been presented in this study. A thorough statistical analysis brings out nanoparticle behavior, where a rapid aggregation of particles (79±13 nm at 0 h to 1464±80 nm at 48 h), with a decrease in specific surface area (32 m2/g at 0 h to 1.7 m2/g at 48 h) was observed. Ion release profile indicated a significant increase in soluble aluminum concentration only after 36 h (277±15 µg/L at 0 h to 462±3 µg/L at 36 h) which reduced over a period of 60 days (279±20 µg/L). A differential response at 1000 µg/L concentration was observed, short term exposure (5 days) showed an immediate effect on the resident algal population (â¼25% decreased viability) and the long term (7 months/210 days) exposure showed a gradual recovery. Thus, nanomaterials may not have the stipulated toxic response, at low concentration and longer standing period, presumably owing to the complexity of the natural systems.
Asunto(s)
Óxido de Aluminio/química , Monitoreo del Ambiente , Agua Dulce/química , Nanopartículas/química , Aluminio/análisis , Óxido de Aluminio/análisis , Óxido de Aluminio/metabolismo , Óxido de Aluminio/toxicidad , Chlorophyta/efectos de los fármacos , Hidrodinámica , Concentración de Iones de Hidrógeno , Nanopartículas/análisis , Nanopartículas/toxicidad , Nanopartículas/ultraestructura , Factores de Tiempo , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/toxicidadRESUMEN
The cytotoxicity of Al(2)O(3) nanoparticles (NP) at very low exposure levels (1 µg/mL and less) to a dominant bacterial isolate from freshwater (lake water), Bacillus licheniformis, was examined. Sterile lake water was directly used as a test medium or matrix to simulate the freshwater environment. Exposure to 1 µg/mL Al(2)O(3) NP for 2 h caused a 17% decrease in cell viability (as determined by plate count and MTT assay). During the test period, the particles were found to be stable against aggregation in the matrix and exerted a nano-size effect on the exposed test organisms. The decrease in cell viability was proven not to be due to the release of Al(3+) ions from the nanoparticles in the dispersion. The zeta potential and FT-IR analyses suggested that the surface charge based attachment of nanoparticles on to the bacterial cell wall was responsible for flocculation leading to toxicity. The cell wall damage confirmed through SEM and the lipid peroxidation assay also contributed toward toxicity. This study warns of possible ecotoxicity of nanoparticles even at environmentally relevant concentrations. However, detailed studies need to be carried out to establish probable mechanistic aspects of this low concentration toxicity phenomenon.
Asunto(s)
Óxido de Aluminio/toxicidad , Bacillus/efectos de los fármacos , Contaminación Ambiental/prevención & control , Agua Dulce/química , Nanopartículas del Metal/toxicidad , Adsorción , Óxido de Aluminio/efectos adversos , Óxido de Aluminio/metabolismo , Bacillus/crecimiento & desarrollo , Bacillus/metabolismo , Adhesión Bacteriana , Pared Celular/efectos de los fármacos , Pared Celular/metabolismo , Relación Dosis-Respuesta a Droga , Peroxidación de Lípido/efectos de los fármacos , Nanopartículas del Metal/química , Microscopía Electrónica de Rastreo , Tamaño de la Partícula , Espectroscopía Infrarroja por Transformada de Fourier , Propiedades de SuperficieRESUMEN
In the present study, silver nanoparticles were rapidly synthesized at room temperature by treating silver ions with the Citrus limon (lemon) extract. The effect of various process parameters like the reductant concentration, mixing ratio of the reactants and the concentration of silver nitrate were studied in detail. In the standardized process, 10(-2)M silver nitrate solution was interacted for 4h with lemon juice (2% citric acid concentration and 0.5% ascorbic acid concentration) in the ratio of 1:4 (vol:vol). The formation of silver nanoparticles was confirmed by Surface Plasmon Resonance as determined by UV-Visible spectra in the range of 400-500 nm. X-ray diffraction analysis revealed the distinctive facets (111, 200, 220, 222 and 311 planes) of silver nanoparticles. We found that citric acid was the principal reducing agent for the nanosynthesis process. FT-IR spectral studies demonstrated citric acid as the probable stabilizing agent. Silver nanoparticles below 50 nm with spherical and spheroidal shape were observed from transmission electron microscopy. The correlation between absorption maxima and particle sizes were derived for different UV-Visible absorption maxima (corresponding to different citric acid concentrations) employing "MiePlot v. 3.4". The theoretical particle size corresponding to 2% citric acid concentration was compared to those obtained by various experimental techniques like X-ray diffraction analysis, atomic force microscopy, and transmission electron microscopy.
Asunto(s)
Biomimética/métodos , Citrus/química , Nanopartículas del Metal/química , Modelos Químicos , Tamaño de la Partícula , Extractos Vegetales/metabolismo , Plata/química , Ácido Cítrico/química , Nanopartículas del Metal/ultraestructura , Microscopía de Fuerza Atómica , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de Fourier , Difracción de Rayos XRESUMEN
Potential health and environmental effects of nanoparticles need to be thoroughly assessed before their widespread commercialization. Though there are few studies on cytotoxicity of nanoparticles on mammalian and human cell lines, there are hardly any reports on genotoxic and cytotoxic behavior of nanoparticles in plant cells. This study aims to investigate cytotoxic and genotoxic impacts of silver nanoparticles using root tip cells of Allium cepa as an indicator organism. A.cepa root tip cells were treated with four different concentrations (25, 20, 75, and 100 ppm) of engineered silver nanoparticles (below 100 nm size) dispersion, to study endpoints like mitotic index, distribution of cells in mitotic phases, different types of chromosomal aberrations, disturbed metaphase, sticky chromosome, cell wall disintegration, and breaks. For each concentration five sets of microscopic observations were carried out. No chromosomal aberration was observed in the control (untreated onion root tips) and the mitotic index (MI) value was 60.3%. With increasing concentration of the nanoparticles decrease in the mitotic index was noticed (60.30% to 27.62%). The different cytological effects including the chromosomal aberrations were studied in detail for the treated cells as well as control. We infer from this study that silver nanoparticles could penetrate plant system and may impair stages of cell division causing chromatin bridge, stickiness, disturbed metaphase, multiple chromosomal breaks and cell disintegration. The findings also suggest that plants as an important component of the ecosystems need to be included when evaluating the overall toxicological impact of the nanoparticles in the environment.
Asunto(s)
Nanopartículas del Metal/toxicidad , Mutágenos/toxicidad , Cebollas/efectos de los fármacos , Plata/toxicidad , Aberraciones Cromosómicas/inducido químicamente , Índice Mitótico , Raíces de Plantas/efectos de los fármacos , Pruebas de ToxicidadRESUMEN
Silver nanoparticles are known to have bactericidal effects. A new generation of dressings incorporating antimicrobial agents like silver nanoparticles is being formulated to reduce or prevent infections. The particles can be incorporated in materials and cloth rendering them sterile. Recently, it was found that aqueous silver ions can be reduced by aqueous extract of plant parts to generate extremely stable silver nanoparticles in water. Apart from being environmentally friendly process, use of Neem leaves extract might add synergistic antibacterial effect of Neem leaves to the biosynthesized nanoparticles. With this hypothesis the biosynthetic production of silver nanoparticles by aqueous extract of Neem leaves and its bactericidal effect in cotton cloth against E. Coli were studied in this work. Silver nanoparticles were synthesized by short-term (1 day) and long-term (21 days) interaction of Neem extract (20% w/v) and 0.01 M AgNO3 solution in 1:4 mixing ratio. The synthesized particles were characterized by UV visible spectroscopy, transmission electron microscopy, and incorporated into cotton disks by (i) centrifuging the disks with liquid broth containing nanoparticles, (ii) in-situ coating process during synthesis, and (iii) coating with dried and purified nanoparticles. The antibacterial property of the nanoparticles coated cotton disks was studied by disk diffusion method. The effect of consecutive washing of the coated disks with distilled water on antibacterial property was also investigated. This work demonstrates the possible use of biologically synthesized silver nanoparticles by its incorporation in cloths leading them to sterilization.