Semi-Automatic Spectral Image Stitching for a Compact Hybrid Linescan Hyperspectral Camera towards Near Field Remote Monitoring of Potato Crop Leaves.

The miniaturization of hyperspectral cameras has opened a new path to capture spectral information. One such camera, called the hybrid linescan camera, requires accurate control of its movement. Contrary to classical linescan cameras, where one line is available for every band in one shot, the latter asks for multiple shots to fill a line with multiple bands. Unfortunately, the reconstruction is corrupted by a parallax effect, which affects each band differently. In this article, we propose a two-step procedure, which first reconstructs an approximate datacube in two different ways, and second, performs a corrective warping on each band based on a multiple homography framework. The second step combines different stitching methods to perform this reconstruction. A complete synthetic and experimental comparison is performed by using geometric indicators of reference points. It appears throughout the course of our experimentation that misalignment is significantly reduced but remains non-negligible at the potato leaf scale.

Comparative study of membrane potential-sensitive fluorescent probes and their use in ion channel screening assays.

In this study, the authors compared and evaluated 4 membrane potential probes in the same cellular assay: the oxonol dye DiBAC(4)(3), the FLIPR membrane potential (FMP) dye (Molecular Devices), and 2 novel fluorescence resonance energy transfer (FRET) dye systems from PanVera [CC2-DMPE/DiSBAC(2)(3)] and Axiom [DiSBAC(1)(3)/DiSBAC(1)(5)]. The kinetic parameters of each membrane probe were investigated in RBL-2H3 cells expressing an endogenous inward rectifier potassium channel (IRK1). The FMP dye presented the highest signal over background ratio whereas the FRET dyes from PanVera gave the fastest response. The determination of IC(50) values for 8 different channel modulators indicated a good correlation between the 4 membrane probe systems. The compound-dye interaction was evaluated in the presence of compounds at 10 muM and clearly indicated no effect on the FMP or the PanVera donor dye, whereas some major interference with the oxonol probes was observed. Using a cell permeabilization assay in the presence of gramicidin, the authors concluded that the FRET dyes from PanVera and the FMP dye are unable to measure the gramicidin-induced cell membrane hyperpolarizations. The 4 dye systems were investigated under high-throughput screening (HTS) conditions, and their respective Z' parameter was determined. The characteristics of each dye system and its potential use in HTS assays is discussed.