Inclusion of chia seeds (Salvia hispanica L.) and pumpkin seeds (Cucurbita moschata) in dairy sheep diets.

Chia (Salvia hispanica L.) seed (CS) and Pumpkin (Cucurbita moschata) seed (PS) are used in ruminant diets as energy sources. The current experiment studied the impact of dietary inclusion of CS and PS on nutrient intake and digestibility, milk yield, and milk composition of dairy sheep. Twelve primiparous Texel × Suffolk ewes [70 ± 5 days in milk (DIM); 0.320 ± 0.029 kg milk yield] were distributed in a 4 × 3 Latin square design and fed either a butter-based control diet [CON; 13 g/kg dry matter] or two diets with 61 g/kg DM of either CS or PS. Dietary inclusion of CS and PS did not alter live weight (p >0.1) and DM intake (p >0.1). However, compared to the CON, dietary inclusion of both CS and PS increased the digestibility of neutral detergent fiber (p <0.001) and acid detergent lignin (p < 0.001). Milk production (p = 0.001), fat-corrected milk (p < 0.001), and feed efficiency (p < 0.001) were enhanced with PS, while the highest milk protein yield (p < 0.05) and lactose yield (p < 0.001) were for CS-fed ewes. Compared to the CON diet, the ingestion of either CS and/or PS decreased (p < 0.001) the C16:0 in milk. Moreover, both CS and PS tended to enhance the content of C18:3n6 (p > 0.05) and C18:3n3 (p > 0.05). Overall short-term feeding of CS and/or PS (up to 6.1% DM of diet) not only maintains the production performance and digestibility of nutrients but also positively modifies the milk FA composition.

Effect of Adding Extra Virgin Olive Oil to Hair Sheep Lambs' Diets on Productive Performance, Ruminal Fermentation Kinetics and Rumen Ciliate Protozoa.

This study determined productive performance, ruminal fermentation kinetics and rumen ciliate protozoa in hair sheep lambs fed different levels of olive oil. Twenty-four growing lambs were used, with an initial live weight of 10.5 ± 2.9 kg, and randomly assigned into four treatments (six animals per treatment) containing increasing levels of extra virgin olive oil (0, 2, 4 and 6% of dry matter). Animals were fed for 80 days, and sampling was carried out weekly. Intake of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and metabolizable energy (ME) differed between treatments (p < 0.05), with a linear and cubic tendency to decrease when oil concentrations were increased. Digestibility coefficients of OM, CP and NDF were not affected; however, the relationship between total intake and nutrient digestibility (DM, OM, NDF, ADF) increased with 2% DM olive oil. Compared with all treatments, the concentration of propionic acid increased by 16% with 4% olive oil. The intake of olive oil did not affect the protozoa population and live weight gain. Overall, the inclusion of olive oil in low concentrations (2% of DM) positively influences feed intake and nutrient digestibility in hair sheep lambs.

Herbal Vitamin C Prevents DNA Oxidation and Modifies the Metabolomic Water Profile of Tilapia (Oreochromis spp.).

This experiment aimed to evaluate the effects of herbal vitamin C at different levels on tilapia (Oreochromis spp.) growth, potential DNA damage, and the metabolomic profile of water effluent. Forty-five tilapias were housed in separate plastic tanks (80 L), and these were randomly assigned to three treatments: (a) a commercial diet (CD) only; (Nutripec Purina®); (b) the commercial diet plus 250 mg of herbal vitamin C (HVC)/kg (CD250); and (c) the commercial diet plus 500 mg of HVC/kg (CD500). Biometric measurements were taken each week, blood samples were collected from the caudal vein on the final day, and water effluent was taken each week and immediately frozen (-80 °C) until further analysis (gas chromatography/mass spectrometry (GC/MS) systems). Data were completely randomized with a 2 × 2 factorial arrangement of treatments. Upon including herbal vitamin C, the final BW (p = 0.05) and BWG (p = 0.06) increased linearly. Herbal vitamin C decreases DNA damage (p ≥ 0.05). PLS-DA showed a 41.6% variation between treatments in the water samples. Fifteen metabolites had the best association between treatments, with a stronger correlation with CD500. Herbal vitamin C could improve fish performance, prevent DNA damage, and influence changes in the metabolomic profile of the water.