Alkali-catalyzed hydrothermal oxidation treatment of triclosan in soil: Mechanism, degradation pathway and toxicity evaluation.

The continuous accumulation of chlorinated organic pollutants in soil poses a potential threat to ecosystems and human health alike. Alkali-catalyzed hydrothermal oxidation (HTO) can successfully remove chlorinated organic pollutants from water, but it is rarely applied to soil remediation. In this work, we assessed this technique to degrade and detoxify triclosan (TCS) in soil and we determined the underlying mechanisms. The results showed a dechlorination efficiency of TCS (100 mg per kg soil) of 49.03 % after 120 min reaction (H2O2/soil ratio 25 mL·g-1, reaction temperature 180 °C in presence of 1 g·L-1 NaOH). It was found that soil organic constituents (humic acid, HA) and inorganic minerals (SiO2, Al2O3, and CaCO3) suppressed the dechlorination degradation of TCS, with HA having the strongest inhibitory effect. During alkali-catalyzed HTO, the TCS molecules were effectively destroyed and humic acid-like or fulvic acid-like organics with oxygen functional groups were generated. Fluorescence spectroscopy analysis showed that hydroxyl radicals (OH) were the dominant reactive species of TCS degradation in soil. On the basis of the Fukui function and the degradation intermediates, two degradation pathways were proposed. One started with cleavage of the ether bond between the benzene rings of TCS, followed by dechlorination and the opening of benzene via oxidation. The other pathway started with direct hydroxylation of the benzene rings of TCS, after which they were opened and dechlorinated through oxidation. Analysis of the soil structure before and after treatment revealed that the soil surface changed from rough to smooth without affecting soil surface elements. Finally, biotoxicity tests proved that alkali-catalyzed HTO effectively reduced the toxicity of TCS-contaminated soil. This study suggests that alkali-catalyzed hydrothermal oxidation provides an environmentally friendly approach for the treatment of soil contaminated with chlorinated organics such as TCS.

Anti-hyperglycemic effects and mechanism of traditional Chinese medicine Huanglian Wan in streptozocin-induced diabetic rats.

ETHNOPHARMACOLOGICAL RELEVANCE: Huanglian Wan (HLW) is a prescription of traditional Chinese medicine (TCM), which has been used to treat diabetes mellitus for thousands of years in China. In this study we provide experimental evidence for the clinical use of HLW in the treatment of diabetes mellitus. MATERIALS AND METHODS: HLW extract was prepared and the main components (namely berberine and catalpol) contained in the extract were assayed with high performance liquid chromatography (HPLC), and diabetic model rats were induced by intraperitoneal injection of streptozotocin (STZ). After grouped randomly, diabetic rats were administered low or high dose of HLW extract, acarbose and vehicle for 33 days, respectively. Body weight, food intake, urine volume, urine sugars, fasting plasma glucose and fasting plasma insulin were monitored to evaluate its antidiabetic effects in diabetic rats. Intestinal mucosa homogenate was prepared and the activities of intestinal disaccharidases were assayed. Moreover, oral sucrose tolerance test (OSTT) was performed and the inhibitory effect of HLW extract on the maltase and sucrase in vitro was evaluated. RESULTS: After the treatment of HLW extract, the body weight and the fasting plasma insulin level were found to be increased while food intake, urine volume, urine sugars and fasting plasma were decreased. OSTT showed that HLW extract could lower the postprandial plasma glucose level of diabetic rats. Furthermore, HLW extract could inhibit the activities of sucrase and maltase in vitro. CONCLUSIONS: According to our present findings, the TCM prescription HLW possessed potent anti-hyperglycemic effect on STZ-induced diabetic rats. And HLW extract exerted anti-hyperglycemic effect partly via inhibiting the increased activities of intestinal disaccharidases and elevating the level of plasma insulin in diabetic rats induced by streptozotocin.

Polysaccharide from Gynura divaricata modulates the activities of intestinal disaccharidases in streptozotocin-induced diabetic rats.

During diabetes, structural and functional changes in the alimentary tract are known to take place resulting in an increased absorption of intestinal glucose and alterations in the activities of brush-border disaccharidases. To elucidate the effect of administrating polysaccharide from Gynura divaricata (PGD) on disaccharidase activities, the specific activities of intestinal disaccharidases, namely sucrase, maltase and lactase, were measured in streptozotocin-induced diabetic rats. Normal control and diabetic rats were treated by oral administration with PGD. Specific activities of intestinal disaccharidases were increased significantly during diabetes, and amelioration of the activities of sucrase and maltase during diabetes was clearly visible by the treatment with PGD. However, the increased activity of lactase during diabetes mellitus was remarkably alleviated by the administration of PGD only in the duodenum. Meanwhile, oral sucrose tolerance tests demonstrated that PGD alleviated the hyperglycaemia during diabetes mellitus, resulting from the amelioration in the activities of intestinal disaccharidases. The present investigation suggests that PGD exerted an anti-diabetic effect partly via inhibiting the increased intestinal disaccharidase activities of diabetic rats. This beneficial influence of administration of PGD on intestinal disaccharidases clearly indicates their helpful role in the management of diabetes.