Effect of Qinglongyi Polysaccharides on Complex Mobility of Erythrocytes in S180 Mice / 中草药·英文版

Objective: To study the effect of Qinglongyi polysaccharides (QP) in the exocarp of Juglans mandshurica on the complex mobility of erythrocytes in S180 mice. Methods: Erythrocytes were collected and prepared into suspensions, and the complex mobility of cells was measured using high performance capillary electrophoresis (HPCE). Optimized experimental conditions were as follows: 50 cm × 75 μm capillary, buffer for electrophoresis; phosphate solution containing hydroxypropylmethyl cellulose (0.1 mol/L, pH 7.4), injection pressure 3.448 kPa, injection time 10 s, separation voltage 20 kV, and column temperature 25 °C. Results: The migration time of erythrocytes in S180 mice was longer than that in normal mice, which was 18.09 min for the model group and 12.11 min for the control group, and the complex mobility of erythrocytes in S180 mice was lower than that in normal mice, which was 0.92 × 10-4 cm2/(Vs) for the model group and 1.38 × 10-4 cm2/(Vs) for the control group. It was also found that S180 mice treated by QP could shorten the migration time and increase the complex mobility of erythrocytes. Conclusion: QP could improve the complex mobility of erythrocytes in S180 mice, and HPCE could be used as a powerful tool for determining the physiological state and functions of erythrocytes. © 2013 Tianjin Press of Chinese Herbal Medicines.

Study on the effects of two kinds of cactus polysaccharide on erythrocyte immune function of S180 mice / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effects of two kinds of cactus polysaccharide on erythrocyte immune function in S180 mice.</p><p><b>METHOD</b>Classical pharmaceutical method and test kit.</p><p><b>RESULT</b>The cactus polysaccharide increased the content of RBC-CaR, RFER, decreased the content of RFIR, raised the content of sialic acid. And the effect of median dose group of medical cactus polysaccharide and high dose group of edible cactus polysaccharide is very remarkable (P < 0.01) compared with model group.</p><p><b>CONCLUSION</b>The cactus polysaccharide improved the erythrocyte function of tumor-mice, which may be one of anti-tumor mechanisms.</p>

Study on the effects of cool and hot ethanol extracts from qinglongyi on the biochemical function of tumor membrane in H22 mice / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effects of cool and hot ethanol extracts from Qinglongyi on tumor membrane protein content, lipid fluidity, and membrane close capability in H22 mice.</p><p><b>METHOD</b>The membrane protein content, by, lipid fluidity and membrane close capability were measured by means of SDS-PAGE, skinitzky assay and Zamudio method respectively.</p><p><b>RESULT</b>The cool and hot ethanol extracts from Qinglongyi decreased the content of tumor membrane protein, the lipid fluidity and membrane close capability.</p><p><b>CONCLUSION</b>The cool and hot ethanol extracts from Qinglongyi can change the biochemical substance and biochemical function to cause disaggregation and death of the tumor cell, which may be one of the mechanisms underlying the anti-tumor of Qinglongyi.</p>

Study on the effects of two kinds of cactus polysaccharide on erythrocyte membrane protein and fluidity of the lipid in S180 mice / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effects of two kinds of cactus polysaccharide on Band 3 protein, cross-linking protein and lipid fluidity of erythrocyte membrane in S180 mice.</p><p><b>METHOD</b>The membrane protein content was analysed by SDS-PAGE. Lipid fluidity was measured by Skinitzky method.</p><p><b>RESULT</b>The two kinds of cactus polysaccharide increased the content of Band 3 protein and decreased the content of cross-linking protein, raised the lipid fluidity. While the effect of median dose group of medical cactus polysaccharide is very remarkable (P < 0.01), the effect of high dose group of edible cactus polysaccharide is very remarkable (P < 0.01).</p><p><b>CONCLUSION</b>By improving the erythrocyte membrane function of tumor-mice, they enhanced the immune function, which may be one of anti-tumor mechanisms.</p>