Effects of bamboo leaf extract on growth performance, meat quality, and meat oxidative stability in broiler chickens.

This study was conducted to investigate the effects of dietary bamboo leaf extract (BLE) on growth performance, meat quality, oxidative stability, and nuclear factor erythroid 2-related factor 2 (Nrf2) related gene expression of breast meat in broilers. A total of 576 one-day-old male Arbor Acres broilers were divided into 6 groups. The control group (CTR) was fed basal diet, while BLE1, BLE2, BLE3, BLE4, and BLE5 were fed basal diet supplemented with 1.0, 2.0, 3.0, 4.0, and 5.0 g BLE per kg feed, respectively. Compared with the CTR group, BLE2 and BLE5 increased average daily feed intake from 1 to 21 D and 22 to 42 D (P < 0.05), BLE1 and BLE2 improved average daily gain (ADG) and feed to gain ratio from 22 to 42 D (P < 0.05). Throughout the trial period, the highest body weight and favorable ADG and feed to gain ratio were observed in the BLE2 group. The drip loss at 24 h and pH at 45 min postmortem of breast meat were linearly improved by BLE supplementation (P < 0.05). Shear force was significantly lower in BLE2 and BLE3 than that in CTR group. Increasing supplementation of BLE linearly improved free radical scavenging capacity and decreased malondialdehyde content of breast meat during 12 D of storage (P < 0.05). Total antioxidant capacity and glutathione peroxidase activity were linearly increased by BLE supplementation (P < 0.05). Compared with the CTR group, the mRNA expression of Nrf2 and glutathione peroxidase in BLE3, BLE4, and BLE5 groups was significantly promoted, and glutathione S-transferase gene expression was increased in BLE2, BLE4, and BLE5 (P < 0.05). The highest (P < 0.05) heme oxygennase-1 gene expression was observed in BLE5. In conclusion, broiler supplemented with BLE improved growth performance and meat quality, BLE supplementation might activate Nrf2 pathway to alleviate lipid oxidation and increase antioxidant capacity of breast meat. The dosage of 2.0 to 3.0 g/kg BLE in broiler diet was recommanded.

Dietary mannan oligosaccharide ameliorates cyclic heat stress-induced damages on intestinal oxidative status and barrier integrity of broilers.

This study investigated protective effects of mannan oligosaccharide (MOS) inclusion on growth performance, intestinal oxidative status, and barrier integrity of cyclic heat-stressed broilers. A total of 240 one-day-old chicks were allocated into 3 treatments of 10 replicates each. Control broilers reared at a thermoneutral temperature were fed a basal diet, whereas broilers in heat stress and MOS groups raised at a cyclic high temperature (32 to 33°C for 8 h/d) were given the basal diet supplemented with 0 or 250 mg/kg MOS, respectively. Compared with control group, heat stress decreased (P < 0.05) average daily gain and feed conversion ratio during grower, finisher, and entire periods, average daily feed intake during finisher and entire periods, and ileal superoxide dismutase activity at 42 D, whereas increased (P < 0.05) rectal temperature at 21 and 42 D and jejunal malondialdehyde content at 42 D. Dietary MOS increased (P < 0.05) average daily gain, average daily feed intake, and feed conversion ratio during finisher and entire periods, but decreased (P < 0.05) jejunal malondialdehyde concentration of heat-stressed broilers at 42 D. Heat stress decreased (P < 0.05) jejunal villus height (VH) and claudin-3 gene expression at 21 D, and VH and VH: crypt depth (CD) ratio in jejunum and ileum as well as mRNA abundances of jejunal mucin 2 and occludin, and ileal mucin 2, zonula occludens-1, and occludin, and claudin-3 at 42 D, whereas increased (P < 0.05) serum D-lactate acid content at 21 and 42 D, and serum diamine oxidase activity and jejunal CD at 42 D. The MOS supplementation increased (P < 0.05) jejunal VH at 21 D, VH and VH: CD of jejunum and ileum at 42 D, mRNA abundances of jejunal occludin and ileal mucin 2, zonula occludens-1, and occludin at 42 D, whereas reduced (P < 0.05) ileal CD at 42 D. These results suggested that MOS improved growth performance, and oxidative status and barrier integrity in the intestine of broilers under cyclic heat stress.

Effects of dietary phytosterols on growth performance, antioxidant status, and meat quality in Partridge Shank chickens.

To investigate the effects of dietary phytosterols (PS) on growth performance, antioxidant status, and meat quality of Partridge Shank chickens, a total of 256 1-day-old male Partridge Shank chicks were randomly assigned into 4 dietary treatments, and each of them replicated 8 times with 8 chicks per replicate. Birds in the 4 treatments were fed a basal diet supplemented with 0 (Control group), 20, 40, and 80 mg/kg PS for 50 D, respectively. Dietary supplementation of PS quadratically increased average daily gain of chickens during the grower and overall periods, whereas linearly decreased the feed-to-gain ratio during the starter period. Compared with control group, a significant increase in average daily gain during the grower and overall periods was observed in chickens fed the basal diet supplemented with 40 mg/kg PS. Increasing PS addition linearly increased serum glutathione peroxidase (GSH-Px) activity at 21 and 50 D and hepatic GSH-Px and superoxide dismutase activities at 21 D, whereas linearly decreased malondialdehyde concentration of breast muscle at 50 D. Meanwhile, 40 mg/kg PS supplementation significantly increased serum GSH-Px activity, and hepatic superoxide dismutase and GSH-Px activities at 21 D as compared with the control. PS supplementation linearly and quadratically decreased drip loss (24 and 48 h postmortem) and luminance value (24 h postmortem) of breast muscle in broilers at 50 D, and there was statistical difference between the control and PS-supplemented group. In conclusion, PS supplementation can improve growth performance, antioxidant status, and meat quality of Partridge Shank chickens, with its optimum level in Partridge Shank chickens' diet being 40 mg/kg.

Betaine improves the growth performance and muscle growth of partridge shank broiler chickens via altering myogenic gene expression and insulin-like growth factor-1 signaling pathway.

This study was conducted to investigate the effect of betaine on growth performance, carcass characteristics, myogenic gene expression, and insulin-like growth factor-1 (IGF-1) signaling pathway in partridge shank broiler chickens. A total of 192 one-day-old partridge shank broiler chickens were randomly divided into 4 groups with 6 replicates of 8 chickens for a 52-d feeding trial. Broilers were fed a basal diet supplemented with 0 (control), 250 (B250), 500 (B500), or 1,000 (B1000) mg/kg betaine. Compared with the control group, the B500 and B1000 groups had higher (P < 0.05) body weight gain (BWG), and the B500 group had a lower (P < 0.05) feed/gain ratio (F:G) during the whole trial period. Moreover, the B1000 group increased (P < 0.05) the breast muscle yield and decreased (P < 0.05) relative abdominal fat weight. The mRNA expression of myocyte enhancer factor 2B (MEF2B) and mechanistic target of rapamycin (mTOR) and mTOR phosporylation were higher (P < 0.05) in both breast and thigh muscles in the B500 and B1000 groups than those in the control group. The higher (P < 0.05) concentration and mRNA expression of IGF-1 were also observed in breast muscle in the B500 and B1000 groups. Additionally, the B1000 group up-regulated (P < 0.05) the mRNA level of myogenic differentiation factor 1 (MyoD1) in breast muscle and myogenin (MyoG) in thigh muscle. In conclusion, diets supplemented with 500 or 1,000 mg/kg betaine improved the growth performance of partridge shank broiler chickens during the whole trial period, and the B1000 group significantly improved the breast muscle growth. These improvements might result from increased mRNA expression of MyoD1 and MEF2B in breast muscle and MyoG and MEF2B in thigh muscle, and through alterations in IGF-1/mTOR signaling pathway.

Effects of Modified Palygorskite Supplementation on Egg Quality and Mineral Element Content, and Intestinal Integrity and Barrier Function of Laying Hens.

This study was conducted to investigate effects of modified palygorskite (MPal) supplementation on the laying performance, egg quality and mineral element content, immunity, oxidative status, and intestinal integrity and barrier function of laying hens. A total of 360 52-week-old Hyline Brown hens were randomly assigned into four dietary treatments for a 7-week feeding trial. The birds were fed a basal diet supplemented with 0 (control group), 0.25, 0.5, and 1 g/kg MPal, respectively. The supplementation of MPal did not alter laying performance and egg quality among groups. Compared with the control group, MPal inclusion decreased lead (Pb) content in yolks at 49 days, and either 0.5- or 1-g/kg MPal supplementation decreased Pb accumulation in yolks at 25 days and manganese (Mn) accumulation in yolks at 25 and 49 days. The contents of jejunal secretory immunoglobulin A (SIgA), ileal SIgA, and immunoglobulin G were decreased by the dietary 0.5-g/kg MPal supplementation. The supplementation of MPal also decreased malondialdehyde content in jejunum and ileum, and decreased serum diamine oxidase activity of the laying hens at 25 and 49 days. The inclusion of 0.5 and 1 g/kg MPal enhanced villus height in jejunum and ileum, and also increased the ratio of villus height to crypt depth in ileum. In conclusion, MPal supplementation decreased Pb and Mn contents in yolks, and exhibited beneficial effects on the intestinal immunity, oxidative status, and intestinal integrity and barrier function of laying hens and its optimal dosage was 0.5 g/kg.

Evaluation of Dietary Palygorskite Supplementation on Growth Performance, Mineral Accumulations, Antioxidant Capacities, and Meat Quality of Broilers Fed Lead-Contaminated Diet.

The objective of this study was to evaluate influence of dietary palygorskite (Pal) supplementation on growth performance, mineral accumulations in the tissues (livers, kidneys, and muscles), antioxidant capacities, and meat quality of broilers fed lead (Pb)-contaminated diet. One-hundred forty-four male broiler chicks were randomly divided into three treatment groups, receiving a corn-soybean meal basal diet (the control group), the basal diet contaminated with 10 mg/kg Pb (the Pb group), and the basal diet with 10-g/kg Pal supplementation and 10-mg/kg Pb contamination (the Pal/Pb group) from 1 to 42 days of age, respectively. Treatments did not affect growth performance of broilers in the 42-day study (P > 0.05). Compared with the control group, Pb contamination increased Pb accumulation in the livers, kidneys, and muscles (P < 0.05); elevated malondialdehyde accumulation in the livers, kidneys, and breast muscles; glutathione peroxidase activity in the livers and superoxide dismutase activity in the kidneys (P < 0.05); exacerbated drip loss in the pectoralis muscles (P < 0.05); and reduced glutathione peroxidase activity in the pectoralis muscles (P < 0.05) of broilers at 42 days of age. The values of these parameters were reversed in the Pal/Pb group to levels comparable with those in the control group (P < 0.05). Additionally, Pal supplementation reduced redness value in the pectoralis muscles (P < 0.05), and decreased Cu concentration in the pectoralis muscles and livers at 42 days of age as well as its accumulation in the kidneys at both 21 and 42 days of age compared with the other two groups (P < 0.05). The results suggested that dietary Pal supplementation would decrease Pb residue in the tissues, alleviate oxidative stress, and affect meat quality of broilers exposed to Pb.

Effects of threonine supplementation on the growth performance, immunity, oxidative status, intestinal integrity, and barrier function of broilers at the early age.

The aim of this study was to investigate effects of L-threonine (L-Thr) supplementation on the growth performance, immunity, antioxidant status, and intestinal health of broilers at the early age. One hundred and forty-four 1-day-old male broiler chicks (Arbor Acres Plus) were allocated into 3 treatments with 6 replicates of 8 birds each, and fed a basal diet (analyzed Thr content, 7.87 g/kg) supplemented with 0 (control diet), 1 and 3 g/kg L-Thr for 21 d, respectively. Treatments did not alter growth performance of broilers. Compared with control, 1 g/kg Thr supplementation increased relative weight of spleen (P = 0.013). A higher level of Thr (3 g/kg) increased relative weight of thymus (P = 0.003). The supplementation of 3 g/kg Thr reduced Escherichia coli (P = 0.040) and Salmonella colonies (P = 0.015), whereas increased Lactobacillus colonies (P < 0.001) in the cecal contents. Thr supplementation increased intestinal villus height (P < 0.05), and the ratio of villus height to crypt depth (P < 0.001), and the values for these parameters were intermediate with 1 g/kg Thr. Goblet cell density was increased by Thr supplementation (P < 0.001). The jejunal immunoglobulin G content was increased by the inclusion of Thr (P = 0.002). Broilers fed diet supplemented with 1 g/kg Thr exhibited increased concentrations of jejunal immunoglobulin M (P = 0.037) and secretory immunoglobulin A (P = 0.018). Likewise, 3 g/kg Thr inclusion increased ileal secretory immunoglobulin A content (P = 0.023). The jejunal malondialdehyde accumulation was reduced by Thr inclusion (P = 0.012). A higher level of Thr inclusion also reduced malondialdehyde content in the serum (P = 0.029). The high level of Thr inclusion (3 g/kg) upregulated mucin-2 mRNA expression (P = 0.034), whereas downregulated the mRNA abundances of interferon-γ (P = 0.036) and interleukin-1ß (P = 0.031) in the ileum. In conclusion, L-Thr supplementation can improve immunity, antioxidant capacity, and intestinal health of broilers at an early age.

Effects of Palygorskite Inclusion on the Growth Performance, Meat Quality, Antioxidant Ability, and Mineral Element Content of Broilers.

The present study was conducted to investigate different levels of palygorskite supplementation on the growth performance, meat quality, muscular oxidative status, and mineral element accumulation of broilers. One hundred ninety-two 1-day-old Arbor Acres broiler chicks were allocated to four dietary treatments with six replicates of eight chicks per replicate. Birds in the four treatments were given a basal diet supplemented with 0, 5, 10 and 20 g/kg palygorskite for 42 days, respectively. Compared with the control group, neither 5 g/kg nor 10 g/kg palygorskite inclusion affected growth performance of broilers during the 42-day study (P > 0.05). However, the highest level of palygorskite supplementation at 20 g/kg increased feed/gain ratio (F/G) of broilers (P < 0.001). Yellowness (P < 0.001) and redness (P = 0.003) of breast muscle and yellowness of leg muscle (P = 0.001) were decreased by palygorskite supplementation at the levels of 10 g/kg and especially 20 g/kg. In addition, redness of leg muscle was also reduced by the inclusion of 20 g/kg palygorskite (P = 0.009). In contrast, malonaldehyde (MDA) accumulation in the breast muscle was significantly increased by 20 g/kg palygorskite supplementation (P < 0.001). Supplementation of palygorskite at either 10 or 20 g/kg significantly decreased lead (Pb) accumulation in the breast (P = 0.001) or thigh (P = 0.045) and copper (Cu) accumulation in the breast (P = 0.022). In conclusion, growth performance, meat color, and antioxidant capacity of meat would reduce with the increasing level of palygorskite supplementation, whereas a higher level of palygorskite (10 or 20 g/kg) can alter mineral element accumulations in muscles as evidenced by reduced muscular Cu and Pb contents.

An evaluation of zinc bearing palygorskite inclusion on the growth performance, mineral content, meat quality, and antioxidant status of broilers.

The current study was conducted to investigate the effect of zinc (Zn) bearing palygorskite (ZnPal) inclusion on the growth performance, mineral content, meat quality, and antioxidant status of broilers. A total of 240 one-day-old Arbor Acres broiler chicks were randomly allocated into 5 dietary treatments with 6 replicates of 8 chicks. Broilers in the 5 treatments were fed a basal diet supplemented with 0, 20, 40, 60, and 80 mg/kg Zn diet in the form of ZnPal for 42 d, respectively. Birds exhibited similar average daily gain (ADG), average daily feed intake (ADFI), and feed/gain ratio (F:G) among groups during the 42-day study (P>0.05). ZnPal supplementation linearly increased iron (Fe) (P=0.031) and magnesium (Mg) (P=0.002) content in the pectoralis major muscle. Similarly, the inclusion of ZnPal tended to increase Zn content in the thigh (P=0.072) and linearly increase Zn content in the pectoralis major muscle (P=0.055). The concentration of copper (Cu) in the thigh was linearly decreased by ZnPal inclusion (P=0.011). Meanwhile, a quadratic trend for reduced Cu content was observed in the pectoralis major muscle (P=0.074) and thigh (P=0.082), respectively. The supplementation of ZnPal linearly reduced cooking loss in the pectoralis major muscle (P=0.013), and linearly (P=0.029) and quadratically (P=0.034) decreased cooking loss in the thigh. Malondialdehyde (MDA) concentration in the thigh was linearly (P=0.020) and quadratically (P=0.017) reduced by ZnPal inclusion. Additionally, ZnPal supplementation tended to linearly enhance total antioxidant capacity (T-AOC) activity of the pectoralis major muscle (P=0.083). The results obtained in the current study indicated that ZnPal inclusion could alter muscular mineral accumulation, improve meat quality, and enhance the muscular antioxidant capacity of broilers, and Zn supplementation in the form of ZnPal at the dosage of 20 mg/kg would be sufficient in improving meat quality and muscular oxidative status.

The value of angiography in diagnosis of Meckel's diverticulum: case report.

Meckel's diverticulum is the most common anomaly of the gastrointestinal tract and usually produces no symptoms, although the classic symptom is painless rectal bleeding in a child. Because most symptomatic diverticula in children contain ectopic gastric tissue, radionuclide imaging using a technetium 99m pertechnetate scan is considered to be the method of choice. However, a bleeding Meckel's diverticulum may not be detected by pertechnetate imaging despite the presence of ectopic gastric mucosa. When the scan is negative, visceral angiography is an alternative procedure for detecting Meckel's diverticulum. We encountered a case of pathology-proven Meckel's diverticulum whose angiography result was diagnostic, while upper gastrointestinal panendoscopy, colonofibroscopy, barium enema studies, and repeated radionuclide scans were all negative. We report on this case to emphasize the potential role for angiographic detection of Meckel's diverticulum.