RESUMEN
Five new flavonoid C-glycosides named desmodinosides A-E (1-5) and one known compound, apigenin 6-C-ß-d-xylopyranosyl-2''-O-ß-D-glucopyranoside (6) have been isolated from the methanol extract of the aerial parts of Desmodium heterocarpon var. stigosum. These compounds were determined by 1D and 2D-NMR and HR-MS spectroscopies. The methanol extract of this plant, in particular, demonstrated hepatoprotection and antifungal inhibition. This extract has a remarkable hepatoprotection and activity-dose response with an EC50 of 43.07 µg/mL. The hepatoprotective effect on human liver hepatoma cells (HepG2) of the isolated flavonoid C-glycosides 1-6 was observed. Desmodinosides A-C (1-3) were found to exhibit moderate hepatoprotective activity on HepG2 cells. Of these, compound 2 showed the best hepatoprotective activity with an EC50 value of 74.12 µg/mL. While compounds 1 and 3 displayed EC50 values of 271.21 and 211.99 µg/mL, respectively. Quercetin, a positive control, also caused an EC50 value of 36.42 µg/mL. In addition to having hepatoprotective effect, the methanol extract had an inhibitory effect on the growth of oomycete; it inhibited Phytophthora infestans with IC50 of 13.3 µg/mL and IC90 of 78.7 µg/mL. The oomycete inhibition was directly attributed to compounds 5 and 6, which significantly inhibited P. infestans with IC50 values of 27.4 and 24.7 µg/mL, respectively. Both 5 and 6 and methanol extract were active against P. infestanse in a dose-dependent manner. Our study demonstrated for the first time the new flavonoid C-glycosides from D. heterocarpon var. stigosum and their novel pharmacological properties. The study findings also suggest the plant extract and its metabolites could be used as a new botanical source of bioactive compounds.
Asunto(s)
Antifúngicos , Flavonoides , Humanos , Antifúngicos/farmacología , Metanol , Estructura Molecular , Glicósidos , Extractos Vegetales/químicaRESUMEN
Alternaria porri (Ellis) Clf. causes purple blotch disease on Allium plants which results in the reduction of crop yields and quality. In this study, to efficiently find natural antifungal compounds against A. porri, we optimized the culture condition for the spore production of A. porri and the disease development condition for an in vivo antifungal assay. From tested plant materials, the methanol extracts derived from ten plant species belonging to the families Cupressaceae, Fabaceae, Dipterocarpaceae, Apocynaceae, Lauraceae, and Melastomataceae were selected as potent antifungal agents against A. porri. In particular, the methanol extract of Caryodaphnopsis baviensis (Lec.) A.-Shaw completely inhibited the growth of A. porri at a concentration of 111 µg/ml. Based on chromatographic and spectroscopic analyses, a neolignan compound magnolol was identified as the antifungal compound of the C. baviensis methanol extract. Magnolol showed a significant inhibitory activity against the spore germination and mycelial growth of A. porri with IC50 values of 4.5 and 5.4 µg/ml, respectively. Furthermore, when magnolol was sprayed onto onion plants at a concentration of 500 µg/ml, it showed more than an 80% disease control efficacy for the purple blotch diseases. In terms of the antifungal mechanism of magnolol, we explored the in vitro inhibitory activity on individual oxidative phosphorylation complexes I-V, and the results showed that magnolol acts as multiple inhibitors of complexes I-V. Taken together, our results provide new insight into the potential of magnolol as an active ingredient with antifungal inhibitory action to control purple blotch on onions.
Asunto(s)
Alternaria/efectos de los fármacos , Antifúngicos/farmacología , Compuestos de Bifenilo/farmacología , Lauraceae/química , Lignanos/farmacología , Cebollas/microbiología , Enfermedades de las Plantas/microbiología , Extractos Vegetales/farmacología , Metanol/química , Micelio/efectos de los fármacos , Micelio/crecimiento & desarrolloRESUMEN
In the search for new natural resources showing plant disease control effects, we found that the methanol extract of Polyalthia longifolia suppressed fungal disease development in plants. To identify the bioactive substances, the methanol extract of P. longifolia was extracted by organic solvents, and consequently, four new 2-oxo-clerodane diterpenes (1-4), a new 4(3 â 2)-abeo-clerodane diterpene (5), together with ten known compounds (6-16) were isolated and identified from the extracts. Of the new compounds, compound 2 showed a broad spectrum of antifungal activity with moderated minimum inhibitory concentration (MIC) values in a range of 50-100 µg/mL against tested fungal pathogens. Considering with the known compounds, compound 6 showed the most potent antifungal activity with an MIC value in the range of 6.3-12.5 µg/mL. When compound 6 was evaluated for an in vivo antifungal activity against rice blast, tomato late blight, and pepper anthracnose, compound 6 reduced the plant disease by at least 60% compared to the untreated control at concentrations of 250 and 500 µg/mL. Together, our results suggested that the methanol extract of twigs and leaves of P. longifolia and its major compound 6 could be used as a source for the development of eco-friendly plant protection agents.
Asunto(s)
Diterpenos de Tipo Clerodano , Polyalthia , Antifúngicos/farmacología , Diterpenos de Tipo Clerodano/farmacología , Pruebas de Sensibilidad Microbiana , Extractos Vegetales/farmacología , Hojas de la PlantaRESUMEN
While searching for new antifungal compounds, we revealed that a methanol extract of plant species Maesa japonica has a potent antifungal activity in vivo against rice blast fungus Magnaporthe oryzae. To identify the antifungal substances, the methanol extract of M. japonica was extracted by organic solvents, and consequently, six active compounds were isolated from the n-butanol layer. The isolated compounds were five new acylated triterpenoid saponins including maejaposide I (1), maejaposides C-1, C-2, and C-3 (2-4), and maejaposide A-1 (5), along with a known one, maejaposide A (6). These chemical structures were determined by NMR and a comparison of their NMR and MS data with those reported in the literature. Based on the in vitro antifungal bioassay, the five compounds (2-6) exhibited strong antifungal activity against M. oryzae with MIC values ranging from 4 to 32 µg/mL, except for maejaposide I (1) (MIC > 250 µg/mL). When the compounds were evaluated at concentrations of 125, 250, and 500 µg/mL for an in vivo antifungal activity against rice blast, compounds 2-6 strongly reduced the development of blast by at least 85% to 98% compared to the untreated control. However, compound 1 did not show any in vivo antifungal activity up to a concentration of 500 µg/mL. Taken together, our results suggest that the methanol extract of M. japonica and the new acylated triterpenoid saponins can be used as a source for the development of natural fungicides.
Asunto(s)
Fungicidas Industriales , Maesa/química , Magnaporthe/efectos de los fármacos , Oryza/microbiología , Extractos Vegetales/farmacología , Saponinas/farmacología , Acilación , Espectroscopía de Resonancia Magnética , Estructura Molecular , Extractos Vegetales/química , Saponinas/química , Saponinas/aislamiento & purificación , Triterpenos/aislamiento & purificación , Triterpenos/farmacologíaRESUMEN
Plants contain a number of bioactive compounds that exhibit antimicrobial activity, which can be recognized as an important source of agrochemicals for plant disease control. As part of our search for new antimicrobial agents from natural sources, we found that a crude methanol extract of Trevesia palmata exhibited a promising antifungal activity against phytopathogenic fungi, such as Magnaporthe oryzae and Botrytis cinerea. Furthermore, based on activity-guided fractionation, we isolated five antifungal compounds from the methanol extract of T. palmata: two new triterpene glycosides (TPGs), TPG1 (hederagenin-3-O-ß-D-glucopyranosyl-(1 â 3)-α-L-rhamnopyranosyl-(1 â 2)-α-L-rhamnopyranosyl-(1 â 2)-α-L-arabinopyranoside) and TPG5 (3-O-α-L-rhamnopyranosyl asiatic acid), along with three known TPGs (TPG2 [macranthoside A], TPG3 [α-hederin], and TPG4 [ilekudinoside D]). The chemical structures of the TPGs were determined by spectroscopic analyses and by comparison with literature data. An in vitro antifungal bioassay revealed that except for TPG4 (ilekudinoside D; IC50 >256 µg/ml), the other TPGs exhibited strong antifungal activities against the rice blast pathogen M. oryzae with IC50 values ranging from 2-5 µg/ml. In particular, when the plants were treated with compound TPG1 (500 µg/ml), disease control values against rice blast, tomato grey mold, tomato late blight, and wheat leaf rust were 84, 82, 88, and 70%, respectively, compared to the non-treatment control. Considering the in vitro and in vivo antifungal activities of the TPGs and the T. palmata methanol extracts, our results suggest that T. palmata can be a useful source to develop new natural fungicides.
Asunto(s)
Araliaceae/química , Fungicidas Industriales/aislamiento & purificación , Enfermedades de las Plantas/prevención & control , Triterpenos/aislamiento & purificación , 1-Butanol , Acetatos , Evaluación Preclínica de Medicamentos , Fungicidas Industriales/química , Fungicidas Industriales/farmacología , Glicósidos , Metanol , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/química , Extractos Vegetales/química , Solventes , Triterpenos/química , Triterpenos/farmacologíaRESUMEN
As an alternative to synthetic pesticides, natural materials such as plant extracts and microbes have been considered to control plant diseases. In this study, methanol extracts of 120 plants were explored for in vivo antifungal activity against Rhizoctonia solani, Botrytis cinerea, Phytophthora infestans, Puccinia triticina, and Blumeria graminis f. sp. hordei. Of the 120 plant extracts, eight plant extracts exhibited a disease control efficacy of more than 90% against at least one of five plant diseases. In particular, a methanol extract of Curcuma zedoaria rhizomes exhibited strong activity against wheat leaf rust caused by P. triticina. When the C. zedoaria methanol extracts were partitioned with various solvents, the layers of n-hexane, methylene chloride, and ethyl acetate showed disease control values of 100, 80, and 43%, respectively, against wheat leaf rust. From the C. zedoaria rhizome extracts, an antifungal substance was isolated and identified as a sesquiterpene ketolactone based on the mass and nuclear magnetic resonance spectral data. The active compound controlled the development of rice sheath blight, wheat leaf rust, and tomato late blight. Considering the in vivo antifungal activities of the sesquiterpene ketolactone and the C. zedoaria extracts, these results suggest that C. zedoaria can be used as a potent fungicide in organic agriculture.
Asunto(s)
Curcuma/química , Fungicidas Industriales/farmacología , Lactonas/farmacología , Enfermedades de las Plantas/prevención & control , Extractos Vegetales/farmacología , Sesquiterpenos/farmacología , Acetatos/química , Basidiomycota/efectos de los fármacos , Basidiomycota/patogenicidad , Fungicidas Industriales/química , Hexanos , Lactonas/química , Lactonas/aislamiento & purificación , Solanum lycopersicum/microbiología , Metanol/química , Estructura Molecular , Agricultura Orgánica/métodos , Oryza/microbiología , Extractos Vegetales/química , Rhizoctonia/efectos de los fármacos , Rhizoctonia/patogenicidad , Rizoma/química , Sesquiterpenos/química , Sesquiterpenos/aislamiento & purificación , Solventes/química , Triticum/microbiologíaRESUMEN
We previously isolated Nicotiana benthamiana matrix metalloprotease 1 (NMMP1) from tobacco leaves. The NMMP1 gene encodes a highly conserved, Zn-containing catalytic protease domain that functions as a factor in the plant's defense against bacterial pathogens. Expression of NMMP1 was strongly induced during interactions between tobacco and one of its pathogens, Phytophthora infestans. To elucidate the role of the NMMP1 in defense of N. benthamiana against fungal pathogens, we performed gain-of-function and loss-of-function studies. NMMP1-overexpressing plants had stronger resistance responses against P. infestans infections than control plants, while silencing of NMMP1 resulted in greater susceptibility of the plants to the pathogen. This greater susceptibility correlated with fewer NMMP1 transcripts than the non-silenced control. We also examined cell death as a measure of disease. The amount of cell death induced by the necrosis-inducing P. infestans protein 1, PiNPP1, was dependent on NMMP1 in N. benthamiana. Potato plants overexpressing NMMP1 also had enhanced disease resistance against P. infestans. RT-PCR analysis of these transgenic potato plants revealed constitutive up-regulation of the potato defense gene NbPR5. NMMP1-overexpressing potato plants were taller and produced heavier tubers than control plants. We suggest a role for NMMP1in pathogen defense and development.
Asunto(s)
Resistencia a la Enfermedad , Metaloproteinasa 1 de la Matriz/genética , Nicotiana/genética , Phytophthora infestans/fisiología , Enfermedades de las Plantas/genética , Proteínas de Plantas/genética , Solanum tuberosum/inmunología , Metaloproteinasa 1 de la Matriz/inmunología , Enfermedades de las Plantas/inmunología , Enfermedades de las Plantas/microbiología , Proteínas de Plantas/inmunología , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/inmunología , Plantas Modificadas Genéticamente/microbiología , Solanum tuberosum/genética , Solanum tuberosum/microbiología , Nicotiana/inmunología , Nicotiana/microbiología , Regulación hacia ArribaRESUMEN
In the search for new antibacterial agents from natural sources, we revealed that a crude methanol extract of Sapium baccatum was highly active against Ralstonia solanacearum, a causal agent of a serious disease called bacterial wilt of tomato. The bioassay-guided fractionation of this extract resulted in the isolation of seven known active compounds, including gallic acid, methyl gallate, corilagin, tercatain, chebulagic acid, chebulinic acid, and quercetin 3-O-α-L-arabinopyranoside. Their chemical structures were determined by electrospray ionization mass spectrometry and nuclear magnetic resonance spectroscopy. An in vitro antibacterial bioassay using a broth microdilution method revealed that, except for quercetin 3-O-α-L-arabinopyranoside (MIC = 250 µg/mL), the isolated compounds exhibited strong antibacterial activity against R. solanacearum (MIC = 26-52 µg/mL). Among the seven compounds, methyl gallate exhibited the strongest broad-spectrum activity against most of the plant pathogenic bacteria tested (MIC = 26-250 µg/mL). In the in vivo experiments, the crude extract of S. baccatum at 2000 and 1000 µg/mL reduced the development of tomato bacterial wilt by 83 and 63%, respectively, under greenhouse conditions after 14 days of infection. The results suggested that the extracts of S. baccatum or isolated tannins could be used as natural bactericides for the control of bacterial wilt of tomato.
Asunto(s)
Antibacterianos/farmacología , Enfermedades de las Plantas/microbiología , Enfermedades de las Plantas/prevención & control , Ralstonia solanacearum/efectos de los fármacos , Sapium/química , Solanum lycopersicum/microbiología , Taninos/farmacología , Antibacterianos/química , Antibacterianos/aislamiento & purificación , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/farmacología , Ralstonia solanacearum/fisiología , Taninos/química , Taninos/aislamiento & purificaciónRESUMEN
BACKGROUND: Medicinal plants are widely used for the treatment of different infectious diseases. Infectious diseases caused by bacteria have a large impact on public health. This study aimed to determine the in vitro antibacterial activity of the medicinal plants traditionally used in Vietnam against the bacterial strains associated with infectious diseases. METHODS: Methanol extracts of twelve Vietnamese medicinal plants were tested for their antibacterial activity against five bacterial species including Gram-positive bacteria (Bacillus cereus, Bacillus subtilis, and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) using the broth microdilution method. RESULTS: All the plant extracts showed antibacterial activity, especially against Gram-positive bacteria (Bacillus cereus, Bacillus subtilis, and Staphylococcus aureus). Baeckea frutescens extract revealed a potent activity against the Gram-positive bacteria with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 62.5 µg/ml. High activity against all the three Gram-positive bacteria was also observed for the extracts of Cratoxylum formosum ssp. pruniflorum, Pogostemon cablin, and Pedilanthus tithymaloides with MICs of 125, 125 and 250 µg/ml and MBCs of 125-250, 125-250 and 250-500 µg/ml, respectively. The extracts of C. formosum ssp. pruniflorum and P. tithymaloides showed a broad-spectrum antibacterial activity against all the bacteria tested with the MICs of 125-2,000 µg/ml. CONCLUSION: This study indicates clear evidence supporting the traditional use of the plants in treating infectious diseases related to bacteria. In particular, these plant species showed moderate to high antibacterial activity against the Gram-positive bacteria tested.
Asunto(s)
Antibacterianos/farmacología , Extractos Vegetales/farmacología , Plantas Medicinales/química , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Humanos , Pruebas de Sensibilidad Microbiana , VietnamRESUMEN
The methanol extract of the aerial part of Triumfetta grandidens (Tiliaceae) was highly active against Meloidogyne incognita, with second-stage juveniles (J2s) mortality of 100% at 500 µg/mL at 48 h post-exposure. Two 4-quinolone alkaloids, waltherione E (1), a new alkaloid, and waltherione A (2), were isolated and identified as nematicidal compounds through bioassay-guided fractionation and instrumental analysis. The nematicidal activities of the isolated compounds against M. incognita were evaluated on the basis of mortality and effect on egg hatching. Compounds 1 and 2 exhibited high mortalities against J2s of M. incognita, with EC50 values of 0.09 and 0.27 µg/mL at 48 h, respectively. Compounds 1 and 2 also exhibited a considerable inhibitory effect on egg hatching, which inhibited 91.9 and 87.4% of egg hatching, respectively, after 7 days of exposure at a concentration of 1.25 µg/mL. The biological activities of the two 4-quinolone alkaloids were comparable to those of abamectin. In addition, pot experiments using the crude extract of the aerial part of T. grandidens showed that it completely suppressed the formation of gall on roots of plants at a concentration of 1000 µg/mL. These results suggest that T. grandidens and its bioactive 4-quinolone alkaloids can be used as a potent botanical nematicide in organic agriculture.
Asunto(s)
4-Quinolonas/farmacología , Antinematodos/farmacología , Componentes Aéreos de las Plantas/química , Triumfetta/química , Tylenchoidea/efectos de los fármacos , 4-Quinolonas/química , 4-Quinolonas/aislamiento & purificación , Alcaloides/aislamiento & purificación , Alcaloides/farmacología , Animales , Antinematodos/química , Antinematodos/aislamiento & purificación , Estructura Molecular , Agricultura Orgánica , Enfermedades de las Plantas/parasitología , Enfermedades de las Plantas/prevención & control , Extractos Vegetales/farmacologíaRESUMEN
Two new pregnane glycosides, kidjoranine 3-O-ß-D-glucopyranosyl-(1 â 4)-ß-D-glucopyranosyl-(1 â 4)-α-L-cymaropyranosyl-(1 â 4)-ß-D-cymaropyranosyl-(1â4)-α-L-diginopyranosyl-(1 â 4)-ß-D-cymaropyranoside (5) and caudatin 3-O-ß-D-glucopyranosyl-(1 â 4)-ß-D-glucopyranosyl-(1 â 4)-α-L-cymaropyranosyl-(1 â 4)-ß-D-cymaropyranosyl-(1 â 4)-α-L-diginopyranosyl-(1 â 4)-ß-D-cymaropyranoside (6), were isolated from the roots of Cynanchum wilfordii along with four known compounds (1-4). The antifungal activities of the six compounds against barley powdery mildew caused by Blumeria graminis f. sp. hordei were compared to the antifungal activity of polyoxin B. The caudatin glycosides (1, 4, and 6) showed stronger antifungal activities than polyoxin B, whereas kidjoranine glycosides (2, 3, and 5) had weaker activities than polyoxin B. A wettable powder-type formulation (C. wilfordii-WP20) of the ethyl acetate extract from C. wilfordii roots prohibited the development of barley powdery mildew much more effectively than the commercial fungicide polyoxin B-WP10. In addition, C. wilfordii-WP20 effectively controlled strawberry powdery mildew caused by Sphaerotheca humuli under greenhouse conditions. Thus, the crude extract containing the pregnane glycosides can be used as a botanical fungicide for the environmentally benign control of powdery mildews.
Asunto(s)
Antifúngicos/farmacología , Ascomicetos/efectos de los fármacos , Cynanchum/química , Glicósidos/farmacología , Enfermedades de las Plantas/microbiología , Extractos Vegetales/farmacología , Pregnanos/farmacología , Antifúngicos/química , Antifúngicos/aislamiento & purificación , Ascomicetos/fisiología , Glicósidos/química , Glicósidos/aislamiento & purificación , Estructura Molecular , Extractos Vegetales/aislamiento & purificación , Raíces de Plantas/química , Pregnanos/química , Pregnanos/aislamiento & purificaciónRESUMEN
The methanol extract of Annona squamosa seeds was highly active against two phytoparasitic nematodes, Bursaphelenchus xylophilus and Meloidogyne incognita. It efficiently suppressed plant diseases, caused by Phytophthora infestans and Puccinia recondita. Ten annonaceous acetogenins (AAs) were isolated, and their chemical structures were identified by mass and nuclear magnetic resonance spectral data. Out of 10 substances, eight displayed strong in vitro nematicidal activity against B. xylophilus with LD(50) values ranging 0.006 to 0.048 µg/mL. Squamocin-G showed potent nematicidal activity against M. incognita. Squamocin, squamocin-G, and squamostatin-A also displayed potent in vitro and in vivo antifungal activities against P. infestans causing tomato late blight. In addition, squamostatin-A effectively controlled the development of wheat leaf rust caused by P. recondita. Our findings suggested that A. squamosa seeds and its bioactive AAs can be an alternative resource of a promising botanical nematicide and fungicide to control various plant diseases.
Asunto(s)
Acetogeninas/farmacología , Annona/química , Antinematodos/farmacología , Fungicidas Industriales/farmacología , Semillas/química , Acetogeninas/química , Acetogeninas/aislamiento & purificación , Extractos Vegetales/químicaRESUMEN
To develop a natural fungicide against Botrytis cinerea and Colletotrichum gloeosporioides, a total of 25 essential oils were tested for their fumigant activity against post-harvest pathogens. The vaporous phases of oils were treated to each fungus on potato dextrose agar medium in half-plate separated Petri plates at 10 microg per plate. The essential oil of Illicium verum strongly inhibited the mycelial growth of both B. cinerea and C. gloeosporioides by over 90%. On the other hand, the essential oil of Schizonepeta tenuifolia showed inhibitory activity against mycelial growth of only B. cinerea by over 90%. Gas chromatography-mass spectrometry and bioassay indicated trans-anethole in I. verum and menthone in S. tenuifolia as a major antifungal constituent. The essential oils of I. verum and S. tenuifolia and their major constituents could be used to manage post-harvest diseases caused by B. cinerea and C. gloeosporioides.
Asunto(s)
Botrytis/efectos de los fármacos , Colletotrichum/efectos de los fármacos , Illicium/química , Lamiaceae/química , Aceites de Plantas/farmacología , Antifúngicos/metabolismo , Antifúngicos/farmacología , Botrytis/crecimiento & desarrollo , Botrytis/metabolismo , Colletotrichum/crecimiento & desarrollo , Colletotrichum/metabolismo , Cromatografía de Gases y Espectrometría de Masas , Aceites Volátiles/farmacologíaRESUMEN
BACKGROUND: In a search for plant extracts with potent in vivo antifungal activity against various plant diseases, we found that treatment with a methanol extract of Myristica fragrans Houttyn (nutmeg) seeds reduced the development of various plant diseases. The objectives of the present study were to isolate and determine antifungal substances from My. fragrans and to evaluate their antifungal activities. RESULTS: Three antifungal lignans were isolated from the methanol extract of My. fragrans seeds and identified as erythro-austrobailignan-6 (EA6), meso-dihydroguaiaretic acid (MDA) and nectandrin-B (NB). In vitro antimicrobial activity of the three lignans varied according to compound and target species. Alternaria alternata, Colletotrichum coccodes, C. gloeosporioides, Magnaporthe grisea, Agrobacterium tumefaciens, Acidovorax konjaci and Burkholderia glumae were relatively sensitive to the three lignans. In vivo, all three compounds effectively suppressed the development of rice blast and wheat leaf rust. In addition, EA6 and NB were highly active against the development of barley powdery mildew and tomato late blight, respectively. Both MDA and NB also moderately inhibited the development of rice sheath blight. CONCLUSION: This is the first study to demonstrate the in vitro and in vivo antifungal activities of the three lignans from My. fragrans against plant pathogenic fungi.
Asunto(s)
Antifúngicos/farmacología , Lignanos/farmacología , Myristica/química , Plantas/microbiología , Antifúngicos/química , Antifúngicos/aislamiento & purificación , Guayacol/análogos & derivados , Guayacol/química , Guayacol/aislamiento & purificación , Guayacol/farmacología , Lignanos/química , Lignanos/aislamiento & purificación , Espectrometría de Masas , Pruebas de Sensibilidad Microbiana , Semillas/químicaRESUMEN
Methanol extracts of fresh materials of 183 plants were screened for in vivo antifungal activity against Magnaporthe grisea, Corticium sasaki, Botrytis cinerea, Phytophthora infestans, Puccinia recondita and Erysiphe graminis f sp hordei. Among them, 33 plant extracts showed disease-control efficacy of more than 90% against at least one of six plant diseases. The methanol extracts of Achyranthes japonica (whole plant) and Rumex crispus (roots) at concentrations greater than 11 g fresh weight of plant tissue per litre of aqueous Tween 20 solution effectively controlled the development of barley powdery mildew caused by E graminis f sp hordei in an in vivo assay using plant seedlings. At a concentration of 300 g fresh weight of plant tissue per litre of Tween 20 solution, the two extracts were as efficient as the fungicide fenarimol (30 mg litre(-1)) and more active than the fungicide polyoxin B (100 and 33 mg litre(-1)) against Sphaerotheca fuliginea on cucumber plants in glasshouse trials.