RESUMEN
Objective: To establish an HPLC characteristic fingerprint of substance benchmark (standard decoction) of classical famous prescription of Jichuan Decoction (JD), and provide reference for the quality study of substance benchmark of JD. Methods: JD standard decoction was prepared according to the ancient method, 15 batches JD standard decoction were determined by HPLC. The similarity analysis and characteristic peak analysis of 15 batches JD were carried out by the "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2012 version". Results: A total of 18 common characteristic peaks were screened by automatic matching method, peaks 1 and 3 were from Angelicae Sinensis Radix and Cimicifugae Rhizoma, peaks 2, 5, 6, 7, 9, 11 and 13 were from Cistanches Herba, peaks 4, 12, 14, 15 and 17 from were Cimicifugae Rhizoma, peaks 8, 10 and 18 from Aurantii Fructus, and peak 16 was from Angelicae Sinensis Radix. Seven characteristic components were identified by the reference substance, including caffeic acid (peak 1), echinacoside (peak 2), ferulic acid (peak 3), isoferulic acid (peak 4), mullein glycoside (peak 6), naringin (peak 8) and neohesperidin (peak 10). The similarities of 15 batches substance benchmark of JD were greater than 0.9. Conclusion: The HPLC method established for substance benchmark of JD is simple, accurate, stable and sensitive. It can be used for the quality study for JD substance benchmark, and provides a reference for the transformation and development of JD for modern preparations.
RESUMEN
Objective HPLC characteristic fingerprint and chemometrics method were used to compare the chemical characteristics difference between 1-6 years old Cyathula offinalis. Methods The peak area data matrix of 6 × 54 and the difference of characteristic peak of 1-6 years old C. offinalis were analyzed by HPLC. Similarity analysis the samples was investigated with “Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004A”. Hierarchical clustering analysis (HCA) and principal component analysis (PCA) were carried out with SPSS 22.0 software. Results According to the analysis of characteristic peak difference, 1 and 6 years old C. offinalis showed exclusive difference in four chromatographic peaks and five chromatographic peaks, respectively. The peak area of 1 year old sample was significantly higher than other years in four chromatographic peaks, which showed obvious differences in components cumulant. The similarity degree analysis showed that the similarity degree of the 3, 4, and 5 years old C. offinalis were more than 0.98, but the 1, 2 and 6 years old were less than 0.85. Dendrogram of HCA and 3D scatter plot of PCA showed that the 3, 4, and 5 years old C. offinalis were clustered into one class. Conclusion There are certain differences in the chemical components among different growth years of C. offinalis. The chemical components of the 3, 4, and 5 years old C. offinalis tend to be uniform, which provides a scientific reference for the harvest time of C. offinalis.