RESUMEN
T lymphocyte function is strongly depressed in vitro and in vivo under low-g conditions in space as well as simulated in clinostat. Here we describe the effect of a food supplement based on yeast plasmolysate on T cells activated in vitro with Concanavalin A and cultured in a random positioning machine. The mitotic index was measured by 3H-thymidine incorporation into DNA, the expression of activation markers CD25, CD69 and HLA-DR on the cell surface by cytofluorimetry and the secretion of the IL-2R by an enzyme immunoassay. Our data indicate that the food supplement used is capable to modulate T lymphocyte function. The addition of the food supplement increased the expression of activation markers in activated and non-activated cells. Cultivation under low-gravity conditions reduced the expression of the activation markers, but this expression was partly restored or even increased upon addition of yeast plasmolysate. On the other hand, cell proliferation and secretion of soluble IL-2 receptor was reduced after addition of the food supplement in all samples.
Asunto(s)
Suplementos Dietéticos , Activación de Linfocitos/fisiología , Rotación , Saccharomyces cerevisiae , Linfocitos T/metabolismo , Simulación de Ingravidez , Antígenos CD/análisis , Antígenos CD/metabolismo , Antígenos de Diferenciación de Linfocitos T/análisis , Antígenos de Diferenciación de Linfocitos T/metabolismo , División Celular/efectos de los fármacos , Células Cultivadas , Concanavalina A/farmacología , Medios de Cultivo , Citometría de Flujo , Antígenos HLA-DR/análisis , Antígenos HLA-DR/metabolismo , Humanos , Lectinas Tipo C , Índice Mitótico , Receptores de Interleucina-2/análisis , Receptores de Interleucina-2/metabolismo , Linfocitos T/efectos de los fármacos , Linfocitos T/fisiologíaRESUMEN
The effect of a food supplement on immunological parameters of 16 long-distance runners was tested in a randomized, double-blind and placebo-controlled trial. The supplement comprised plasmolysed herbal yeast, malt, honey, and orange juice. No statistically significant differences between the two groups regarding the following variables were detected at three sessions at rest and immediately after a 21 km run: total and differential white blood cell counts, numbers of B- and T-cells and T-subpopulations, concanavalin-A-induced lymphocyte proliferation, serum levels of immunoglobulins, neopterin, IL-2 receptors, beta 2-microglobulin, complement factor b, c4 and c3c, and c1-inactivator. These findings suggest that the effects of the tested food supplement on these parameters are negligible with respect to improvements in the immunological status of long-distance runners. The changes observed immediately after the run had a transient character. In both groups, however, low lymphocyte counts, IgG subclass 2 levels and c1-inactivator levels were noted at rest, which indicate that the immune status of endurance athletes may be affected by training.