Miniaturised marine tests as indicators of aromatic hydrocarbon toxicity: Potential applicability to oil spill assessment.

Assessing oil spill toxicity in real time is challenging due to dynamic field exposures and lack of simple, rapid, and sensitive tests. We investigated the relative sensitivity of two commercially available marine toxicity tests to aromatic hydrocarbons using the target lipid model (TLM). State of the art passive dosing in sealed vials was used to assess the sensitivity of brine shrimp (Artemia franciscana) and rotifer (Brachionus plicatilis). Organisms were exposed to toluene, 1-methylnaphthalene and phenanthrene for 24 h. Toxicity results were analysed using the TLM to estimate the critical target lipid body burden and support comparison to empirical data for 79 other aquatic organisms. Our findings demonstrate the applicability of passive dosing to test small volumes and indicate that the two rapid cyst-based assays are insensitive in detecting hydrocarbon exposures compared to other aquatic species. Our results highlight the limitations of applying these tests for oil pollution monitoring and decision-making.

Current issues confounding the rapid toxicological assessment of oil spills.

Oil spills of varying magnitude occur every year, each presenting a unique challenge to the local ecosystem. The complex, changeable nature of oil makes standardised risk assessment difficult. Our review of the state of science regarding oil's unique complexity; biological impact of oil spills and use of rapid assessment tools, including commercial toxicity kits and bioassays, allows us to explore the current issues preventing effective, rapid risk assessment of oils. We found that despite the advantages to monitoring programmes of using well validated standardised tests, which investigate impacts across trophic levels at environmentally relevant concentrations, only a small percentage of the available tests are specialised for use within the marine environment, or validated for the assessment of crude oil toxicity. We discuss the use of rapid tests at low trophic levels in addition to relevant sublethal toxicity assays to allow the characterisation of oil, dispersant and oil and dispersant mixture toxicity. We identify novel, passive dosing techniques as a practical and reproducible means of improving the accuracy and maintenance of nominal concentrations. Future work should explore the possibility of linking this tiered testing system with ecosystem models to allow the prediction and risk assessment of the entire ecosystem.