RESUMEN
Optimization of the quality and quantity of medicinal materials during cultivation could improve the value of medicinal plants. Light intensity and water availability affect physiological processes and growth of plants. Tulbaghia violacea L. (Alliaceae) bulbs and leaves are widely used traditionally in southern Africa for treatments of many ailments. The interactive effects of light intensity and watering regime on plant growth, nutrient uptake and antifungal activity of T. violacea were evaluated in the current study. Seedlings of T. violacea were grown hydroponically under two shading levels (0% and 40%) while being exposed simultaneously to one of three watering intervals: 5-day, 14-day and 21-day. Different growth parameters (plant height, plant fresh and dry weights) and tissue nutrient contents were evaluated at the end of the experiment. The antifungal activity of acetone extracts on Fusarium oxysporum were evaluated in a microdilution bioassay. Generally, significantly higher concentrations of macronutrients were recorded in the tissue of plants exposed to shorter watering interval. The results showed that different watering frequencies and light intensities significantly (p < 0.05) influenced plant growth parameters (height, and dry and fresh weights). Moreover, there were strong interactive effects of watering frequency and light intensity on most of the plant growth parameters. Remarkably, plants that were simultaneously exposed to the extended watering interval (21-day) and low light intensity showed the best anti-F. oxysporum activity. Key findings of this study revealed that shading alleviated the negative effects of water-deficit stress on plant growth and improved antifungal activity.