[Efficacy of antibiotics and proteolytic enzymes in the treatment of patients with chronic osteomyelitis]. / Effektivnost' lecheniia bol'nykh khronicheskim osteomielitom antibiotikami i proteoliticheskimi fermentami.

The character of the microflora, its antibiotic sensitivity, the titers of lysozyme and beta-lysines, the phagocytic activity of the leucocytes and the number of the T-cells were studied in 52 patients with chronic osteomyelitis before and 5-8 days after the treatment with antibiotics or their combinations with proteolytic enzymes. Pathogenic staphylococci alone or in association were isolated in 92.3 per cent of the patients. The microbiol flora had multiple drug resistance in 80.6 per cent of the cases. It was shown that phagocytosis in the patients was significantly lowered, the lysozyme titer was decreased and the titer of beta-lysines was not markedly changed. As a result of the treatment the lysozyme titer and phagocytosis increased and the number of the T-cells lowered especially in the patients subjected to combined therapy with antibiotics and proteolytic enzymes. The antimicrobial therapy with the use of the enzymes provided a decrease in the bacterial contamination and antibiotic resistance. The results of the study enable prediction of the disease course and therapy efficacy with regard to the immunogenesis, the character of the microflora and its antibiotic resistance.

[Effect of lincomycin, chymotrypsin and their combinations on the splenic plasmacytic reaction and the antibody titer in experimental staphylococcal infection]. / Vliianie linkomitsina i khimotripsina i ikh sochetanii na plazmotsitarnuiu reaktsiiu selezenki i titr antitel pri eksperimental'noi stafilokokkovoi infektsii.

The effect of lincomycin, chymotrypsin and their combinations on the plasmocytic reaction of the spleen and agglutinin titer in mice with experimental staphylococcal infection was studied. The infected mice were divided into 4 groups: the 1st group included untreated infected animals, the 2nd, 3rd and 4th groups consisted of the mice treated with lincomycin, chymotrypsin and their combinations respectively. Lincomycin and chymotrypsin were used in doses of 150 and 2 mg/kg respectively. By the 3rd, 7th, 14th and 21st day of the infection and treatment the animals were decapitated, th blood was collected for determination of the staphylococcal agglutinin titers in the serum, the spleens were removed for investigation of the plasmocytic reaction. It was shown that the treatment of the experimental staphylococcal infection with lincomycin resulted in decreased proliferation of the plasmatic cells and antibody formation. The use of chymotrypsin resulted in increased proliferation of the plasmatic cells and specific antibody titer. The use of chymotrypsin in conjunction with lincomycin lowered the suppressing effect of the latter on the above indices.

[Aspects of the therapeutic action of proteolytic enzymes and antibiotics in experimental staphylococcal infection. The effect of lincomycin, chymotrypsin and their combinations on leukocyte phagocytic activity]. / O nekotorykh aspektakh lechebnogo deistviia proteoliticheskikh fermentov i antibiotikov pri éksperimental'noi stafilokokkovoi infektsii. Vliianie linkomitsina, khimotripsina i ikh sochetanii na fagitsitarnuiu aktivnost' leikotsitov.

The effect of leucomycin, chimotrypsin and their combination on the leucocyte phagocytic activity was studied on mice with experimental staphylococcal infection. Lincomycin and chimotrypsin were administered in doses of 150 and 2 mg/kg respectively. In the study of the leucocyte phagocytic activity it was found on the 3rd, 7th, 14th and 21st days after the beginning of the animal infection and treatment that the experimental staphylococcal infection stimulated the non-specific phagocytosis. Lincomycin inhibited the leucocyte phagocytic activity. The use of chimotrypsin in the process of the staphylococcal infection treatment resulted in increased phagocytosis activity. The combined use of chimotrypsin and leucomycin decreased the inhibitory effect of the latter on phagocytosis.

[Action of lincomycin, chymotrypsin and their combinations on the course of experimental staphylococcal infection]. / Deistvie linkomitsina, khimotripsina i ikh sochetanii na techenie éksperimental'noi stabilokokkovoi infektsii.

The culture of Staphylococcus aureus was administered intraperitoneally in a dose of LD30 to albino mice. The animals of the 1st, 2nd and 3rd groups were treated with lincomycin, chymotripsin and combination of lincomycin with chymotripsin respectively. The animals of the 4th group were used as control and were not subjected to the treatment with the drugs. A part of the animals from every group was killed on the 3rd, 7th, 14th, 21st and subsequent days and their organs were investigated microscopically and bacteriologically. It was found that staphylococci was isolated from the control mice during a 50-day period after inoculation. Complete liberation of the organs from the causative agent within 25 days from the beginning of the experiment was registered in the animals treated with lincomycin. Isolation of the staphylococci was over by the 27th day in the animals treated with chymotrypsin. Liberation of the organs from the causative agent by the 17th day was observed in the albino mice treated with the combination of lincomycin with chymotrypsin. The combined use of lincomycin with chymotrypsin proved to be most effective: no death was registered among the albino mice, the levels of the pathogenicity and antibiotic resistance in the pathogenic staphylococci decreased.