RESUMEN
The present study aimed to establish a pharmacodynamic method using the pySolo software to explore the influence of freeze-dried powders of Shuangxia Decoction (SXD) on the sleep of normal Drosophila melanogaster and the Drosophila melanogaster whose sleep was divested by light. The dose-effect and the time-effect relationships of SXD on sleep were examined. The effect-onset concentration of SXD was 0.25%, the plateau appeared at the concentration of 2.5% and the total sleep time showed a downtrend when the concentration was greater than 2.5%. The sleep time was the longest on the fourth day after SXD was given. The fruit fly sleep deprivation model was repeated by light stimulation at night. The middle dosage group (2.5%) had the best insomnia-curing effect. In conclusion, using the pySolo software, an approach for the pharmacodynamics study was established with Drosophila melanogaster as a model organism to determine the insomnia-curing effects of the traditional Chinese medicine (TCM). Our results demonstrated the reliability of this method. The freeze-dried powders of SXD could effectively improve the sleep quality of Drosophila melanogaster.
Asunto(s)
Animales , Femenino , Humanos , Masculino , Modelos Animales de Enfermedad , Drosophila melanogaster , Medicamentos Herbarios Chinos , Sueño , Trastornos del Inicio y del Mantenimiento del Sueño , QuimioterapiaRESUMEN
The method of monoclonal antibody-based immunoassay has a great importance in the study of quality control of traditional Chinese medicine (TCM) and detection of trace components in vivo animals. Synthesis of small molecule artificial antigen is the prerequisite for the establishment of this method. In present study, catalpol-BSA was synthesized by sodium periodate oxidation method. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry ( MALDI-TOF-MS) and molecular exclusion chromatography showed that catalpol was successfully conjugated with BSA. The mice could specifically produce anti-catalpol antibodies with titer up to 1:8000. The artificial antigen of catalpol was successfully synthesized.
Asunto(s)
Antígenos/inmunología , Glucósidos Iridoides/inmunología , Animales , Anticuerpos/inmunología , Antígenos/química , Inmunoensayo , Glucósidos Iridoides/química , Masculino , Medicina Tradicional China , Ratones , Ratones Endogámicos BALB C , Albúmina Sérica Bovina/química , Albúmina Sérica Bovina/inmunologíaRESUMEN
The present study was designed to isolate and evaluate the antibacterial activity of the compounds from the whole plant of Euphorbia helioscopia L.. Various chromatographic techniques were used to isolate and purify the compound. The structure of the compound was elucidated on basis of spectral data ((1)H NMR, (13)C NMR, (1)H-(1)H COSY, HSQC, HMBC, NOESY, IR, and HR-ESI-MS). A new jatrophone-type diterpenoid (14α,15β-diacetoxy-3β-benzoyloxy-7β-nicotinoyloxy-9-oxo-jatropha-5E,11E-diene), named euphoheliosnoid E (1), was isolated from the whole plant of E. helioscopia L. Compound 1 showed significant anti-microbial activity against oral pathogens.
Asunto(s)
Antiinfecciosos , Farmacología , Diterpenos , Química , Farmacología , Euphorbia , Química , Estructura Molecular , Enfermedades de la Boca , Microbiología , Niacina , Química , Farmacología , Extractos Vegetales , Química , FarmacologíaRESUMEN
AIM@#To investigate the chemical constituents of the aerial part of Euphorbia hirta Linn.@*METHODS@#The chemical constituents were isolated and purified by various chromatographic techniques, and their structures were elucidated on the basis of spectroscopic analysis.@*RESULTS@#Nine compounds were isolated and identified as scopoletin (1), scoparone (2), isoscopoletin (3), quercetin (4), isorhamnetin (5), pinocembrin (6), kaempferol (7), luteolin (8), gallic acid (9).@*CONCLUSION@#Among them compounds 1-3, 5-8 were found from this plant for the first time.