RESUMEN
OBJECTIVE: To analyse a special kind of Schisandra chinensis with the white fruit using ITS2 barcode at molecular levels. METHOD: ITS2 regions were sequenced bidirectionally. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner, MEGA 5.0 software was used to align the sequences. The ITS2 secondary structure was predicted using ITS2 web server, BLAST 1 method was used to identify the S. chinensis with the white fruit. RESULT: The length of the ITS2 sequence was 231 bp. And the sample was identified as S. chinensis using the method of BLAST 1. Their mean interspecific genetic distance (K2P distance) among the populations of the S. chinensis with the white fruit and S. chinensis was far lower than the mean interspecific genetic distance between the S. chinensis and S. sphenanthera. CONCLUSION: By using ITS2 the S. chinensis with the white fruit was identified as S. chinensis, and the ITS2 barcode could be used to identify S. chinensis and S. sphenanthera.
Asunto(s)
ADN de Plantas/genética , ADN Espaciador Ribosómico/genética , Schisandra/química , Schisandra/genética , ADN de Plantas/química , ADN Espaciador Ribosómico/química , Frutas/química , Frutas/clasificación , Frutas/genética , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Schisandra/clasificación , Análisis de Secuencia de ADN , Programas InformáticosRESUMEN
The effect of plant growth regulator forchlorfenuron (CPPU) 1 x 10(-6), 0.67 x 10(-6), 0.5 x 10(-6) on fruit morphology and effective components lignans was studied. Those morphologies were the combination of four basic morphological changes. The result showed, diametre were increased and longitudinal diametre of fruits were inhibited by foliage fertilizers including CPPU. At the same time, 1 000-grain weight and yield showed the varying degrees increase under CPPU. The order of the degree was 0.5 x 10(-6) > 1 x 10(-6) > 0.67 x 10(-6). Six lignans content of Schisandra chinensis of different harvest time and different CPPU processing groups were determined, the results showed that lignans accumulation occurred mainly in periods of premature the half mature fruiting stages. Under the 0.67 x 10(-6) CPPU treatment, schisandrol B, schisandrin B, schisandrin C content of S. chinensis showed different increase.
Asunto(s)
Frutas/efectos de los fármacos , Lignanos/metabolismo , Compuestos de Fenilurea/farmacología , Piridinas/farmacología , Cromatografía Líquida de Alta Presión , Ciclooctanos/análisis , Ciclooctanos/metabolismo , Dioxoles/análisis , Dioxoles/metabolismo , Relación Dosis-Respuesta a Droga , Frutas/crecimiento & desarrollo , Frutas/metabolismo , Lignanos/análisis , Compuestos Policíclicos/análisis , Compuestos Policíclicos/metabolismoRESUMEN
OBJECTIVE: To compare the content of six lignans of different parts of Schisandra chinensis. METHOD: Agilent TC-C18 (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-water gradient system as mobile phase. Wave length was 250 nm. The flow rate was 1 mL x min(-1). Column temperature was 30 degrees C. RESULT: The total lignans content of wild Schisandra chinensis was higher than that of the cultivated varieties. The total lignans content of different parts varied significantly, wherein the root > main branch > side branches > leaf. CONCLUSION: This method is stable, reliable, can be used for the quality evaluation of different parts of Schisandra.