[Use of vitamins for correction of the functional state of cytochrome P450 systems in experimental allergic encephalomyelitis].

It is known that inflammatory cytokines, which level is significantly increased in the pathogenesis of multiple sclerosis (MS), as well as interferon-beta, which is used to treat autoimmune diseases, can inhibit cytochrome P450-dependent processes of detoxification and biotransformation. The uncontrolled decrease of the activity of these processes may have a negative affect on the state of patients, so it is urgent to study the functional state of the cytochrome P450 system and to develop effective means for its regulation in these conditions. The effect of vitamin D3 and efficiency of its composition with vitamins B1, B2, B6, PP, E, alpha-lipoic, alpha-linolenoic acid and mineral substances (Mg, Zn, Se) in prevention of a functional state changes of cytochrome P450- and b5-dependent systems of the rat brain and liver endoplasmic reticulum at EAE are investigated. It has been shown that the essential decrease of the level of these cytochromes is observed both in the brain and liver. In addition the level of activity of NADH- and NADPH-oxidoreductases, which are part of microsomal electron transport chain components and coupled with monooxigenases, was reduced. These changes confirm the disturbances of a redox state and functional activity of detoxication and biotransformation systems in the studied animal tissues. Supplement of vitamin D3 as well as the composition of biologically active substances, which we developed earlier, effectively eliminated the decrease of the level of cytochromes and activities of NADH-oxidoreductase in immunised rat tissues. Normalization of these disturbances can be explained by antioxidant and membrane-stabilizing properties of applied substances, and also by the ability to reduce the activity of inflammatory reactions by regulation of the level of inflammatory cytokines in rat organism at EAE. Thus the studied vitamin-mineral composition appeared to be more effective to normalize the found disturbances and it can be useful for prevention of exacerbations and for improvement of a status of patients with multiple sclerosis and other diseases, which are accompanied with hyperactivation of immune system.

[Theoretical and practical aspects of vitaminology in development of animal husbandry in Ukraine]. / Teoretychni ta praktychni aspekty vitaminologii u rozvytku tvarynnytstva v Ukraïni.

The paper deals with fundamental and applied aspects ofvitaminology development in Ukraine. Its foundations have been laid by A. V. Palladin and R. V. Chagovets, leading scientists of the country, and continued by their followers. Joint efforts of scientists in the field of veterinary, medicine, food branch will permit creating new up-to-date technologies for production of vitamin drugs of a new generation with the corresponding financial support of the state. That is rather necessary for health of the future generation of people.

[Rostyslav Vsevolodovych Chahovets'. Creative activity and scientific school (on the 100th anniversary of birthday)]. / Rostyslav Vsevolodovych Chahovets'. Tvorchyi shliakh ta naukova shkola (do 100-richchia vid dnia narodzhennia).

Herein we review the main stages of life and scientific work of Rostislav Vsevolodovich Chagovets, prominent biochemist and vitaminologist, Member of the Academy of Sciences of Ukrainian SSR, professor and talanted pedagogue. As a founder of scientific school of vitaminologists and researcher in the field of muscle biochemistry, nutrition and vitamins he made a valuable contribution to development of the national and world biochemistry. This work reflects the main trends of fundamental scientific investigations and developments of vitaminological school founded by R. V. Chagovets which underlied the development of contemporary practical vitaminology.

[Study of the effect of a short-chain vitamin E analog on the structural and functional state of the plasma membrane of cardiomyocytes in vitro]. / Vyvchennia vplyvu korotkolantsiuhovoho analoha vitaminu E na strukturno-funktsional'nyi stan membran kardiomiotsytiv in vitro.

The results of study of influence of C6-analog of alpha-tocopherol on the structural and functional state of cardiomyocytes plasma membranes (CPM) in vitro are given. Under condition of incubation of CPM of intact animals the level of phospholipids was decreased, the level of fatty acids was increased, intensity of processes of free-radical oxidation (FRO) was significantly increased and activity of Na+, K(+)-ATPase was decreased in control samples of CPM. The analog of vitamin E supplementation at a concentration of 10 mg/ml of the medium significantly prevented the development of these changes. All of these can be evidence of effective membranes protective action of C6-analog of vitamin E on CPM.