RESUMEN
As extensively active compounds, coumarins are rarely reported on the phytochemistry of the genus Trigonostemon. We herein proposed a fast strategy for analysis and separation of antitumoral active coumarins from the twigs of T. lutescens. Rapid Resolution liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (RRLC-DAD-ESI-MS) analysis indicated the existence of coumarins in the twig extracts. Bioactivity guided phytochemical analysis assays revealed that the twig extract contained some active components that signiï¬cantly inhibited cancer cell viability. Accordingly, a series of coumarins including a new furanocoumarin have been isolated from the twigs of T. lutescens by semi-preparative chromatographic separation. All compounds, especially furan-type coumarins, were reported for the first time from the genus Trigonostemon. The proposed strategy, by combining RRLC-DAD-ESI-MS based and bioactivity guided phytochemical analysis, exemplify a fast method for screening and identifying active components from raw extracts of herbs.
Asunto(s)
Cumarinas/química , Euphorbiaceae/química , Fitoquímicos/química , Extractos Vegetales/química , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Cromatografía Líquida de Alta Presión/métodos , Cumarinas/farmacología , Células HCT116 , Células HeLa , Células Hep G2 , Humanos , Fitoquímicos/farmacología , Extractos Vegetales/farmacología , Espectrometría de Masa por Ionización de Electrospray/métodosRESUMEN
Two new ellagitannins, lutescins A and B (1-2), along with eight known compounds (3-10), were isolated from the twigs of Trigonostemon lutescens. Their structures were elucidated by extensive spectroscopic analyses as well as by comparison with literature data. Compounds 1 and 2 are the first examples of ellagitannins reported in Trigonostemon Genus, and their structures featured a hexahydroxydiphenoyl (HHDP) moiety less often as R configurations in natural products. In addition, all isolated compounds were tested for their inhibitory effects against HeLa, HCT116 and HepG2 cancer cell lines. Compounds 1, 2, 5 showed potent antiproliferative activity, compared with the positive control Cisplatin.
Asunto(s)
Antineoplásicos Fitogénicos/aislamiento & purificación , Euphorbiaceae/química , Taninos Hidrolizables/aislamiento & purificación , Fitoquímicos/aislamiento & purificación , Antineoplásicos Fitogénicos/farmacología , Línea Celular Tumoral , China , Humanos , Taninos Hidrolizables/farmacología , Estructura Molecular , Fitoquímicos/farmacologíaRESUMEN
Three new biphenanthrenes, Liparisphenanthrenes A-C (1-3), along with three known ones were obtained from the ethanolic extract of Liparis nervosa (Orchidaceae) by bioactivity-guided fractionation. Their structures were elucidated on the basis of extensive spectroscopic analysis. All the compounds obtained were tested in vitro for cytotoxic activities against stomach (HGC-27) and colon (HT-29) cancer cell lines. 1, 4 and 5 showed potent cytotoxicities to HGC-27 cell line with IC50 values of 8.21-9.95µmol/L, and 1 and 5 also exhibited potent cytotoxic activities to HT-29 cell line with IC50 values of 8.53-9.27µmol/L.