Cicca acida L.: phytochemistry and pharmacological studies.

OBJECTIVES: Cicca acida L., is reported with traditional and pharmacological uses, and a good number of pure compounds have been isolated from its different parts. But published information is sporadic and fragmentary in nature. Therefore, it is imperative to have a comprehensive account of all of its medicinal potentialities with critical analysis. In this review, the traditional reports, phytochemical and pharmacology studies associated with Cicca acida have been compiled to figure out the net possibilities for its application in modern medicine. KEY FINDINGS: Careful scrutiny reveals that the plant possesses a huge range of medicinal properties, but published report analysis suggests that the plant is effectively used as antibacterial, hepatoprotective, anticonceptive and antidiabetic purposes. The published papers revealed the bioactivity of only 04 compounds. This indicates that a good number of isolated major compounds of this plant are yet to be pharmacologically investigated. CONCLUSION: Intensive study of Cicca acida showed that despite of its plentiful isolated molecules, the effort leading to final product stage seems to be less. Therefore, the plant and its compounds need concentrated effort towards establishment of its therapeutic potentialities.

Ethnomedicinal plants used by traditional healers of North Tripura district, Tripura, North East India.

ETHNOPHARMACOLOGICAL RELEVANCE: Information about ethnomedicinal plants used by traditional healers in Tripura, India is very much limited. As there is rapid erosion of traditional knowledge in the state, needs has arisen to document the diversity of medicinal plants, their preparation and associated diseases. AIM OF THE STUDY: Attempts have been made to explore the herbal medicines used by the traditional healers belonging to the various communities in North Tripura district of Tripura, India and document the associated traditional knowledge on the utilisation of medicinal plants. MATERIALS AND METHODS: The study was based on ethnomedicinal field survey covering a period of 1 year from February 2012 to February 2013. The ethnomedicinal information was collected by using semi-structured questionnaires from different healers and knowledge holders. Collected data were analysed through informant consensus factor, Index of Specialisation and Relative frequency of citation to determine culturally significant plants. RESULTS: A total of 75 species of plants under 68 genera belonging to 43 families were collected during the study for the treatment of 15 disease categories. Leaves were the most frequently used plant parts and most of the medicines were prepared in the form of extract and administered orally. FIC values of the present study indicated that there was a high agreement in the use of plants in the treatment of digestive system disorders and respiratory system disorders among the healers. Plants having high ISu are Justicia adhatoda, Pajanelia longifolia, Catharanthus roseus etc. CONCLUSION: The present study highlighted certain species having higher priority indices for further phytochemical investigation. Counselling of traditional health practitioners should be taken into consideration in order to smooth continuation and extension of traditional medical knowledge and practice for ensuring safe and effective therapy.

Antidiabetic plants used among the ethnic communities of Unakoti district of Tripura, India.

ETHNOPHARMACOLOGICAL RELEVANCE: A large group of ethnic communities living in Unakoti district of Tripura, India is still dependent on traditional herbal remedies for treatment of diabetes. Valuable information collected from these communities in the present investigation is important in maintaining their indigenous knowledge of folklore medicine. METHODS: Systematic and extensive field surveys were conducted during 2011-2013 among the ethnic inhabitants of Unokati district, Tripura, India covering all the seasons to collect information on their traditional herbal medication system for treatment of diabetes. Obtained data were analysed through fidelity level (FL), use value (UV) and relative frequency of citation (RFC) to authenticate the uniqueness of the species being used for diabetes treatment. RESULTS: In this current study a total of 39 medicinal plant species belonging to 37 genera and 28 families were presented, used by the traditional healers of Unakoti district, Tripura, India for diabetes treatment. FL, UV and RFC values of collected plants for the selected study area ranges between 06% and 100%, 0.07% and 2.64% and 0.02% and 0.51% respectively. Out of 39 collected plants, 11, 5 and 3 plant species have showed significant (<50%) FL, UV and RFC values respectively. CONCLUSION: Like many other ethnic communities of the world, inhabitants of Unakoti district depend on a traditional medication system to treat diabetes. Documented floras are locally available and need proper further pharmacological validation to endorse their traditional use in a modern health care system. This will help in the development of effective herbal antidiabetic medicines in near future.

Indigenous knowledge of medicinal plants used by the Reang tribe of Tripura state of India.

ETHNOPHARMACOLOGICAL RELEVANCE: Traditional remedies used for the treatment of various ailments are considered to be very important in the primary health care of Reang people living in Tripura state of Northeast India. Novel information gathered from the present investigation is important in preserving folk indigenous knowledge of Reang tribe. METHODS: Systematic and exhaustive field surveys were conducted during 2003 to 2004 in Reang inhabited areas of Tripura state of Northeast India covering all the seasons, to gather information on medicinal herbs used by them in the treatment of various ailments. Information was collected from 55 traditional herbalists of different age through structured questionnaires and personal observations made during the field visit. The data obtained was analyzed through informant consensus factor (FIC) to determine the homogeneity of informant's knowledge on medicinal plants also the fidelity level (FL) to authenticate the uniqueness of a species to treat a particular ailment. RESULTS: In the present study a total of 125 medicinal plants species belonging to 116 genera and 59 families were presented, used for treating 42 different ailments. The major plant parts used are leaves and most of the remedies are suggested to take orally. The greatest parts of plants used for curing various ailments were found locally. The consensus analysis revealed that the fever and gastro-intestinal diseases have the highest informant consensus factor FIC of 0.79 followed by the dermatological problems (FIC 0.78). It is equal (FIC 0.77) for both general health problems and inflammation and pain while urinogenital problems showed relatively low levels of consensus (FIC 0.63). The level of informants' consent was high for most ailment categories indicating greater homogeneity among informants. In the present study we analyzed the disease categories to highlight some of the important plant species in terms of Fidelity level. Greater parts of the plant species achieve highest fidelity level, while only 4% acquire lower FL. The species with high citation and informant concurrence value are reasonably significant. Cyathea, a rare tree fern used for major cuts or wounds for immediate blood coagulation. Extensive local application may threaten the species if not judiciously managed. CONCLUSION: The traditional pharmacopoeia of the Reang ethnic group incorporates a myriad of diverse flora available locally. Traditional knowledge of the remedies is passed down through oral traditions without any written document. This traditional knowledge is however, currently threatened mainly due to acculturation and deforestation. Therefore, documenting medicinal plants and associated indigenous knowledge can be used as a basis for developing management plans for conservation and sustainable use of medicinal plants of the study area. In addition, findings of this study can be used as an ethnopharmacological basis for selecting plants for future phytochemical and pharmaceutical studies.

Neuroprotective potential of silymarin against CNS disorders: insight into the pathways and molecular mechanisms of action.

Silymarin, a C25 containing flavonoid from the plant Silybum marianum, has been the gold standard drug to treat liver disorders associated with alcohol consumption, acute and chronic viral hepatitis, and toxin-induced hepatic failures since its discovery in 1960. Apart from the hepatoprotective nature, which is mainly due to its antioxidant and tissue regenerative properties, Silymarin has recently been reported to be a putative neuroprotective agent against many neurologic diseases including Alzheimer's and Parkinson's diseases, and cerebral ischemia. Although the underlying neuroprotective mechanism of Silymarin is believed to be due to its capacity to inhibit oxidative stress in the brain, it also confers additional advantages by influencing pathways such as ß-amyloid aggregation, inflammatory mechanisms, cellular apoptotic machinery, and estrogenic receptor mediation. In this review, we have elucidated the possible neuroprotective effects of Silymarin and the underlying molecular events, and suggested future courses of action for its acceptance as a CNS drug for the treatment of neurodegenerative diseases.

Male contraceptive efficacy of Ricinus communis L. extract.

ETHNOPHARMACOLOGICAL RELEVANCE: Ricinus communis L. (Rc), of Euphorbiaceae family is a widespread plant in tropical regions and it is used in traditional medicines as an antifertility agent in India and different parts of the world. AIM OF THE STUDY: The aim of the present study is to revalidate the ethnobotanical knowledge by evaluating the activity of only crude stem bark extracts of Rc. In this study, effects of extracts on male contraceptive efficacy were experimented in vitro with human sperm sample. The work is based on primordial and contemporary therapeutic uses of this plant. MATERIALS AND METHODS: In this study, dose of petroleum ether extract, ethyl acetate extract, acetone extract and lyophilised aqueous extract of Rc were added to fresh human semen in 1:1 volumetric ratio. As the aqueous extract showed a promising result in 1:1 ratio, therefore, the Hypo-osmotic swelling test (HOS), Nuclear chromatin decondensation test (NCD) and Acrosomal status and function test (AFT) were also carried out with the aqueous extract of Rc. RESULTS: The sperm immobilisation effects of the extract appeared immediately in a dose-dependent manner when the samples were treated with four different extracts of this plant. At a concentration of 100mg/mL, 100% (p<0.001 and p<0.05) sperms lost their progressive motility. At a concentration of 300 mg/mL, 100% (p<0.001 and p<0.05) became immotile when treated with aqueous extract. There was 88% (p<0.001 and p<0.05) morphological deformities in sperm sample due the effect of aqueous extract when they were tested for HOS and 91% (p<0.05) sperms behaved against NCD as compared to control group. Also there was a distinct decline (p<0.05) in AFT with increase in dosage concentration. CONCLUSION: The findings of the study revealed that aqueous stem bark extract of the plant showed dose dependent loss of sperm motility by influencing the morphological deformation, blockage in nuclear envelope and distinct declination in acrosomal status of spermatozoa. This research, thus, opens up scope for future exploration of bark of the plant as commercial source of new male contraceptive.