RESUMEN
In this study, the sulfated polysaccharide (SPs) was isolated from Spirulina platensis. The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7⯱â¯0.30%, 21.3⯱â¯0.87%, 7.1⯱â¯0.15% and 7.9⯱â¯0.4% respectively. The elemental analysis confirmed the presence of carbon (18.01⯱â¯0.10%), hydrogen (1.83⯱â¯0.02%) and nitrogen (3.43⯱â¯0.01%). The monosaccharide composition and molecular weight of SPs were analyzed by high-performance liquid chromatography and size exclusion chromatography respectively. The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016â¯kDa. Further, the characterization of SPs was done by UV-visible spectroscopy, X-ray diffraction, FT-IR, 1H NMR and 13C NMR analysis. The obtained SPs exhibited potent antioxidant activity in DPPH (76.45⯱â¯0.49%), reducing power (absorbance: 1.3⯱â¯0.02), hydrogen peroxide scavenging (66.3⯱â¯1.16%), hydroxyl scavenging (68.6⯱â¯3.2%), nitric oxide (81.36⯱â¯1.85%) and total antioxidant (absorbance:1.66⯱â¯0.02) activities at 5â¯mg/ml. In addition, SPs revealed the highest antibacterial efficacy against the pathogenic bacteria Vibrio vulnificus in disc diffusion, agar bioassay and protein leakage assays at 100⯵g/ml. Furthermore, the supplementation of 2% SPs through a feed to the Danio rerio fish enhances the growth and reproductive performances. This finding confirmed that the isolated SPs from S. platensis possess pharmaceutical as well as nutritional properties.