RESUMEN
Two new triterpenoid estersaponins (1, 2) were isolated from the leaves of Pittosporum tobira 'Variegata' (Thunb.) W. T. Aiton, along with one known saponin (3) and one known flavonoid glycoside (4). Their structures were established by different spectroscopic methods including 1D and 2D NMR, UV, as well as ESI-MS analysis. The investigated 80% aqueous methanol extract showed significant dose dependent inhibition of acetic acid induced abdominal writhing in mice. The n-butanol fraction exerted moderate antimicrobial activity against Staphylococcus aureus and Escherichia coli. In addition, it showed in vitro antioxidant activity with IC50 value (7.3 µg/ml) lower than that of the positive control ascorbic acid (11.2 µg/ml), using DPPH free radical scavenging activity method. Evaluation of its in vitro cytotoxicity showed strong activity against breast carcinoma (MCF-7), hepatocellular carcinoma (HepG2), and colon carcinoma (HCT) cell lines.
Asunto(s)
Flavonoides/farmacología , Extractos Vegetales/farmacología , Rosales/química , Saponinas/farmacología , Triterpenos/farmacología , Analgésicos/aislamiento & purificación , Analgésicos/farmacología , Animales , Antibacterianos/aislamiento & purificación , Antibacterianos/farmacología , Antineoplásicos Fitogénicos/aislamiento & purificación , Antineoplásicos Fitogénicos/farmacología , Línea Celular Tumoral , Escherichia coli/efectos de los fármacos , Flavonoides/aislamiento & purificación , Depuradores de Radicales Libres/aislamiento & purificación , Depuradores de Radicales Libres/farmacología , Humanos , Masculino , Ratones , Estructura Molecular , Hojas de la Planta/química , Saponinas/aislamiento & purificación , Staphylococcus aureus/efectos de los fármacos , Triterpenos/aislamiento & purificaciónRESUMEN
A new monodesmosidic spirostanol saponin, along with three known saponins was isolated from Agave macroacantha Zucc leaves. The structure of the new saponin was established as hecogenin-3-O-alpha-L-rhamnopyranosyl (1-->4) beta-D-xylopyranosyl (1-->3)[beta-D-glucopyranosyl (1-->2)] beta-D-glucopyranosyl (1-->4) beta-D-galactopyranoside. The (1)H and (13)C resonances of the four compounds were assigned using a combination of 1D and 2D NMR techniques including (1)H, (13)C, COSY, TOCSY, ROESY, HSQC and HMBC NMR and confirmed by mass spectrometry.
Asunto(s)
Agave/química , Oligosacáridos/aislamiento & purificación , Extractos Vegetales/química , Saponinas/aislamiento & purificación , Espirostanos/aislamiento & purificación , Estructura Molecular , Oligosacáridos/química , Hojas de la Planta , Saponinas/química , Espirostanos/químicaRESUMEN
Fifteen new bidesmosidic triterpenoid saponins (1-15) were isolated from a methanol extract of the leaves of Mimusops laurifolia. Their structures were established using one- and two-dimensional NMR spectroscopy and mass spectrometry and determined to be bidesmosides of protobassic acid (2-4, 11, 12, and 15) and of 16alpha-hydroxyprotobassic acid (1, 5-10, 13, and 14).