Asunto(s)
Arritmias Cardíacas/fisiopatología , Síndrome de Brugada/fisiopatología , Miocitos Cardíacos/metabolismo , Arritmias Cardíacas/genética , Síndrome de Brugada/genética , Evaluación Preclínica de Medicamentos/métodos , Evaluación Preclínica de Medicamentos/estadística & datos numéricos , Edición Génica/métodos , Edición Génica/estadística & datos numéricos , Variación Genética/genética , Variación Genética/fisiología , Humanos , Células Madre Pluripotentes Inducidas/metabolismoRESUMEN
In this paper, the antioxidant capacities and compositions of two commercialized tea products and extracts from Fuzhuan brick tea (FBT) were investigated using three HPLC methods comparing the retention times of injected standards. Principal component analysis and DPPH-spiking HPLC analysis were used to analyze correlation between antioxidant capacity and the compounds detected to screen which compounds contribute to antioxidant activity. Results indicated that all samples contained high amounts of polysaccharides, phenols, and flavonoids and had good antioxidant activity and a high level of correlation among them. Furthermore, gallic acid, epigallocatechin, epicatechin, and epigallocatechin gallate were screened and found to be stronger antioxidant candidates. In summary, the quality of the FBT extracts was not inferior to that of commercialized tea products, suggesting the feasibility that extracts may directly act as instant tea products.
Asunto(s)
Antioxidantes/análisis , Análisis de los Alimentos , Extractos Vegetales/análisis , Té/química , Catequina/análisis , Flavonoides/análisis , Ácido Gálico/análisis , Fenoles/análisis , Polisacáridos/análisisRESUMEN
A study about the ultrasonic extraction, antioxidant capacity and identification of phenolics from Semen Astragali Complanati was undertaken. The optimised extraction condition with an orthogonal experiment design of L9(3(4)) was 50°C, 30min and 15:1 (methanol solution:sample, mL:g). An extraction yield of 40.12±1.10mg gallic acid/g dry seed and the antioxidant capacity of 0.85±0.13mg dry extract/mg DPPH were obtained with the optimum condition, respectively. For each extraction set, the antioxidant capacity by 2,2-diphenyl-1-picrylhydrazyl radical assay highly correlated to its total phenolic content by Folin-Ciocalteu method. Twelve phenolic components obtained with the optimised extraction procedure were tentatively identified by Ultra Performance Liquid Chromatography coupled with Q-TOF Mass Spectrometer (UPLC-Q-TOF) according to their mass spectrometry, UV/vis spectrometry, and related literature reports. Furthermore, the ultrasonic effect on the particles' microstructure of Semen Astragali Complanati was also investigated by scanning electron microscopy (SEM) analysis.