RESUMEN
BACKGROUND AND OBJECTIVE: Hyptis suaveolens is an aromatic plant used in traditional medicine in Burkina Faso for management of various diseases including wounds and inflammatory diseases. Thus, the objective of this work was to characterize the chemical composition, antioxidant and cytotoxic activity of Essential Oil (EO) of H. suaveolens from Burkina Faso on cultured cancer cells. MATERIALS AND METHODS: The chemical composition of EO was determined by GC/FID and GC/MS analysis and the antioxidant activity was evaluated through inhibition of DPPH radicals and ABTS +⢠radical cations. The cytotoxic activity in prostate cancer cells (LNCaP) and cervical cancer cells (HeLa) of EO was evaluated by MTT assay and effect on cells cycle by flow cytometry analysis. RESULTS: A total of 58 compounds were identified in the EO of H. suaveolens of which the major compounds identified are Sabinene 14.03%, ß-Pinene 5.92%, Limonene 4.40%, Eucalyptol 12.78%, Trans-Oxide of Linalol 5.43%, ß-Caryophyllene 11.27%, Germacrene-D 3.04% and Bicyclogermacrene 8.08%. The EO of H. suaveolens showed antioxidant activity and concentration dependent antiproliferative activities with G0/G1 arrest on LNCaP and HeLa cells. CONCLUSIONS: This work help to justify some uses of H. suaveolens in traditional medicine in Burkina Faso and also, presents a promising new application for the essential oil of H. suaveolens in prostate and cervical cancer research.
Asunto(s)
Antioxidantes/farmacología , Hyptis/metabolismo , Neoplasias de la Próstata/metabolismo , Neoplasias del Cuello Uterino/metabolismo , Antioxidantes/química , Burkina Faso , Cationes , Línea Celular Tumoral , Femenino , Citometría de Flujo , Radicales Libres , Cromatografía de Gases y Espectrometría de Masas , Células HeLa , Humanos , Técnicas In Vitro , Concentración 50 Inhibidora , Masculino , Monoterpenos , Aceites Volátiles/farmacología , Extractos Vegetales/farmacología , Sesquiterpenos , Sales de Tetrazolio/farmacología , Tiazoles/farmacologíaRESUMEN
Objectives Natural products commonly used in traditional medicine, such as essential oils (EOs), are attractive sources for the development of molecules with anti-proliferative activities for future treatment of human cancers, e.g., prostate and cervical cancer. In this study, the chemical composition of the EO from Cymbopogon nardus was characterized, as well as its antioxidativeproperties and anti-inflammatory and antiproliferative activities on LNCaP cells derived from prostate cancer. Methods The chemical composition of the EO was determined by GC/FID and GC/MS analyses. The antioxidative properties were assessed using DPPH radical scavenging assay and ABTS+⢠radical cation decolorization assay, and the anti-inflammatory capacity was determined by the inhibition of the lipoxygenase activity. Antiproliferative activity was evaluated by MTT assay. Results Collectively, our data show that the major constituents of C. nardus EO are citronellal (33.06 %), geraniol (28.40 %), nerol (10.94 %), elemol (5.25 %) and delta-elemene (4.09 %). C. nardus EO shows modest antioxidant and anti-inflammatory activity compared to the standard galic acid. C. nardus EO exhibits the best antiproliferative activity on the prostate cancer cell line LNCaP with an IC50 of 58.0 ± 7.9 µg/mL, acting through the induction of the cell cycle arrest. Conclusions This study has determined that C. nardus EO efficiently triggers cytotoxicity and pens a new field of investigation regarding the putative use of this EO in vivo.
Asunto(s)
Antiinflamatorios/farmacología , Antioxidantes/farmacología , Proliferación Celular/efectos de los fármacos , Cymbopogon/química , Aceites Volátiles/farmacología , Monoterpenos Acíclicos/análisis , Monoterpenos Acíclicos/farmacología , Aldehídos/análisis , Aldehídos/farmacología , Línea Celular Tumoral , Ionización de Llama/instrumentación , Cromatografía de Gases y Espectrometría de Masas/instrumentación , Humanos , Medicina Tradicional , Aceites Volátiles/química , Hojas de la Planta/química , Sesquiterpenos/análisis , Sesquiterpenos/farmacologíaRESUMEN
This research highlights the chemical composition, antioxidant, anti-inflammatory and anti-proliferative activities of essential oils from leaves of Ocimum basilicum, Ocimum americanum, Hyptis spicigera, Lippia multiflora, Ageratum conyzoides, Eucalyptus camaldulensis and Zingiber officinale. Essential oils were analyzed by gas chromatography-mass spectrometry and gas chromatography-flame ionization detector. Major constituents were α-terpineol (59.78%) and ß-caryophyllene (10.54%) for Ocimum basilicum; 1, 8-cineol (31.22%), camphor (12.730%), α-pinene (6.87%) and trans α-bergamotene (5.32%) for Ocimum americanum; ß-caryophyllene (21%), α-pinene (20.11%), sabinene (10.26%), ß-pinene (9.22%) and α-phellandrene (7.03%) for Hyptis spicigera; p-cymene (25.27%), ß-caryophyllene (12.70%), thymol (11.88), γ-terpinene (9.17%) and thymyle acetate (7.64%) for Lippia multiflora; precocene (82.10%)for Ageratum conyzoides; eucalyptol (59.55%), α-pinene (9.17%) and limonene (8.76%) for Eucalyptus camaldulensis; arcurcumene (16.67%), camphene (12.70%), zingiberene (8.40%), ß-bisabolene (7.83%) and ß-sesquiphellandrène (5.34%) for Zingiber officinale. Antioxidant activities were examined using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. O. basilicum and L. multiflora exhibited the highest antioxidant activity in DPPH and ABTS tests, respectively. Anti-inflammatory properties were evaluated by measuring the inhibition of lipoxygenase activity and essential oil of Z. officinale was the most active. Anti-proliferative effect was assayed by the measurement of MTT on LNCaP and PC-3 prostate cancer cell lines, and SF-763 and SF-767 glioblastoma cell lines. Essential oils from A. conyzoides and L. multiflora were the most active on LNCaP and PC-3 cell lines, respectively. The SF-767 glioblastoma cell line was the most sensitive to O. basilicum and L. multiflora EOs while essential oil of A. conyzoides showed the highest activity on SF-763 cells. Altogether these results justify the use of these plants in traditional medicine in Burkina Faso and open a new field of investigation in the characterization of the molecules involved in anti-proliferative processes.
Asunto(s)
Antiinflamatorios/química , Antiinflamatorios/farmacología , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/farmacología , Aceites Volátiles/química , Aceites Volátiles/farmacología , Burkina Faso , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Humanos , Aceites de Plantas/química , Aceites de Plantas/farmacologíaRESUMEN
Essential oils extracted from dried leaves of Cupressus dupreziana A. Camus, an endemic species in the Tassili n'Ajjer (Central Sahara of Algeria), were analyzed by gas chomatography coupled to mass spectrometry (GC-MS). Analyses were carried out on 164 trees of 26 natural populations in order to determine the intra-specific variability. Thirty-two terpenoids were identified, the major ones being alpha-pinene (11.5-44.2), delta3-carene (5.7-31.7) and germacrene-D (15.7-54.1). The terpenoid markers used made it possible to determine the individual patterns of chemotypic variability. This variability confirmed that genetic factors were not responsible for the decrease in the number of this species, the main reason probably being the Tassili n'Ajjer desertification.
Asunto(s)
Cupressus/química , Aceites Volátiles/análisis , Cromatografía de Gases y Espectrometría de Masas , Análisis de Componente PrincipalRESUMEN
Different parts of Uvaria ovata (Dunals) A, U. anonoides Baker f. and U. tortilis A. Chev were collected from Ivory Coast, in Toumodi (center), Agboville (south-east) and Sikensi (south), respectively. The essential oils, obtained by hydrodistillation using a Clevenger-type apparatus, were investigated by CG and CG/MS. The proportion of the chromatographed constituents identified varied from 92.5% to 98.5%. For U. ovata, the root bark oil comprised mainly camphene (10.2%), beta-pinene (10.1%), epi-alpha-cadinol (13.2%) and intermedeol (9.7%), while the oil of the stem bark was dominated by epi-alpha-cadinol (27.3%), intermedeol (11.9%) and benzyl benzoate (13.4%). The oil of the leaves showed beta-caryophyllene (15.6%), germacrene D (24.2%) and benzyl benzoate (18.3%) as the most abundant constituents. The leaf oil of U. anonoides was rich in 2,5-dimethoxy-p-cymene (15.5%), bicyclogermacrene (21.3%) and benzyl benzoate (8.7%), while, gamma-terpinene (31.7%), beta-caryophyllene (23.9%) and germacrene D (15.8%) constituted the main components of the stem bark oil of U. tortilis.
Asunto(s)
Aceites Volátiles/química , Uvaria/química , Côte d'Ivoire , Corteza de la Planta/química , Hojas de la Planta/química , Especificidad de la EspecieRESUMEN
The hydrodistilled oils from the aerial parts of Genista ulicina Spach. and G. vepres Pomel., which are endemic to Algeria, were analyzed by gas chromatography-mass spectrometry (GC-MS). In the oil of G. ulicina, 41 compounds were identified representing 90.8% of the total oil, and in G. vepres, 61 compounds representing 84.5% of the total oil. The analyses showed that the major constituents of the oils were lauric acid (14.3%-8.5%), myristic acid (11.5%-5%), linoleic acid (3.1%-11.7%) and palmitic acid (18.6%-26.4%). Using a diffusion method, the oils showed significant antibacterial activities against Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923).