RESUMEN
Identifying the active ingredient from plant extracts and establishing its safety and efficacy remains a challenge, so there is need to develop optimised Isolation and purification method to concentrate the natural product from plant resources. In the key content areas of Pharmaceutical, natural product Industry and food application. Asclepiadaceae perennial herb Calotropis gigantea has a long history of usage in folk medicine. The purpose of this study to isolate, purified and structure elucidation of the saponin compounds. As per the Literature study reported that, the saponin compounds remain a huge source for medicinal, are rich sources of chemical compounds having tremendous diversity with respect to structure, function and mechanism of action. In this study we selected Calotropis gigantea for the separation of the saponin. In the present study we successfully isolated, purified, elucidated the structure and identified the saponin compounds using HPLC and HR LC-MS techniques.