Dual Engineering Interface-Driven Complementary Graphene Oxide-Protein Dimer Supramolecular Architecture Enables Nucleus Imaging and Therapy.

Seeking a versatile nanoplatform for multimodal nucleus imaging and therapy is a challenging task. General complementary bottom-up bionanotechnology for controlling a 3D supramolecular coassembly is proposed. The dual engineering interface proof-of-concept of the supramolecular architecture can be demonstrated via a genetically engineered protein dimer and plasmonically engineered graphene oxide (GO). Incorporation of anisotropic plasmonic nanoparticles as an intercalation layer among the GO 3D supramolecular architecture can provide covalent conjugation sites and simultaneously endow tunable optical properties of GO, ranging from the ultraviolet-to-near-infrared region. Interestingly, the precise design of a specific two-site mutation of the plasmid is favorable for giving an organized coassembly instead of random networks of GO, which contributes to giving continuous distinguishable enhanced Raman imaging for tracking cancer cells. Unexpectedly, penetration into the cell nucleus via the submicro 3D supramolecular coassembly exhibits an excellent nucleus therapeutic potential of cancer cells.

Effects of Fructus ligustri lucidi on the growth, cell integrity, and metabolic activity of the Microcystis aeruginosa.

Agricultural waste has been used in the treatment of cyanobacterial bloom because of its environmental friendly and cost-efficient characteristics. In this work, the effects of Fructus ligustri lucidi (FLL) on the growth inhibition, physiological properties, algicidal property, and cell ultrastructure of Microcystis aeruginosa were investigated for the first time. The alga was efficiently inhibited by FLL at the dosages from 0.25 to 4.0 g L(-1), and the Chl-a fluorescence and metabolic activity of cells also declined gradually. During 25 days incubation time, the inhibition ratio of 0.25 g L(-1) dosage increased from 8 to 68 %, the percentage of intact cells decreased from 94.4 to 59.8 %, the inhibition ratio of 2.0 and 4.0 g L(-1) dosages was nearly 100 %, and the cell membranes were completely broken. The results of Chl-a, propidium iodide (PI) staining, fluorescein diacetate (FDA) staining, and transmission electron microscopy (TEM) assays were consistent with that of growth inhibition tests. The new medium test with the PI staining test suggested that FLL may act as an algicidal agent which can inhibit the growth of M. aeruginosa in the acute time. Consequently, FLL could be an excellent choice in the treatment of eutrophic water.