RESUMEN
The content of pro- and anti-inflammatory cytokines in the hypothalamus, hippocampus, and blood serum of C57Bl/6J mice with depressive-like behavior induced by 20-day social stress was analyzed in 4 h after immune stimulation with LPS (250 µg/kg). These animals are characterized by a tendency to an increase in the blood content of IL-6 and a decrease in the level of IL-10. Changes in cytokine content in the brain of mice with depressive-like state developed under these conditions were observed only in the hippocampus: the levels of IL-1ß, IL-6, TNFα, and IL-10 increased and the content of IFNγ decreased in comparison the corresponding parameters in the controls (not exposed to social stress) and aggressive animals. No changes in the levels of IL-2, IL-4, and IL-17 were revealed in the hypothalamus and hippocampus.
Asunto(s)
Citocinas/metabolismo , Depresión/metabolismo , Hipocampo/metabolismo , Hipotálamo/metabolismo , Animales , Interleucina-10/metabolismo , Interleucina-1beta/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
We studied activity of lysosomal cysteine proteases, cathepsins B and L, in brain structures (frontal cortex, caudate nucleus, hippocampus, and hypothalamus) of C57Bl/6J mice with aggressive and depressive-like behavior formed under conditions of chronic social stress (repeated experience of victories and defeats within 20 days). Mice with depressive-like behavior showed increased activity of cathepsin Ð in the hypothalamus and nucleus caudatus and increased activity of cathepsin L in the hippocampus compared to control animals not subjected to agonistic confrontations. In mice with aggressive behavior, protease activity in the studied brain structures was not changed. In 4 h after immune system activation with LPS (250 µg/kg), cathepsin L activity in the hippocampus of control mice increased in comparison with mice receiving saline. In contrast to control animals, LPS caused a decrease in activity of the enzyme in the caudate nucleus and frontal cortex of aggressive mice and in the hippocampus of mice with depressive-like behavior.
Asunto(s)
Agresión/psicología , Conducta Agonística , Catepsina B/metabolismo , Catepsina L/metabolismo , Depresión/enzimología , Estrés Psicológico/enzimología , Animales , Núcleo Caudado/efectos de los fármacos , Núcleo Caudado/enzimología , Núcleo Caudado/inmunología , Núcleo Caudado/fisiopatología , Depresión/inmunología , Depresión/fisiopatología , Lóbulo Frontal/efectos de los fármacos , Lóbulo Frontal/enzimología , Lóbulo Frontal/inmunología , Lóbulo Frontal/fisiopatología , Hipocampo/efectos de los fármacos , Hipocampo/enzimología , Hipocampo/inmunología , Hipocampo/fisiopatología , Hipotálamo/efectos de los fármacos , Hipotálamo/enzimología , Hipotálamo/inmunología , Hipotálamo/fisiopatología , Lipopolisacáridos/farmacología , Masculino , Ratones , Ratones Endogámicos C57BL , Especificidad de Órganos , Estrés Psicológico/inmunología , Estrés Psicológico/fisiopatologíaRESUMEN
The effect of chronic treatment with antidepressant drugs fluoxetine (20 mg/kg) and imipramine (25 mg/kg) on the number of antibody-producing cells and the main T cell subpopulations in ASC mice characterized by genetic predisposition to depression-like states was studied at the peak of the SE-induced immune response (5×10(8)). Fluoxetine produced an immunostimulatory effect manifested in an increase in the relative and absolute number of IgM antibody-producing cells in the spleen and index of immunoreactivity (CD4/CD8). Administration of fl uoxetine to parental mouse strains without depression (CBA and AKR) had no effect (CBA) or reduced the immune response. The CD4/CD8 ratio did not increase under these conditions. Imipramine was ineffective in the correction of immune reactions in a depression-like state.